Experiment: R2A with 3-octanone 5.3129 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE40_RS28210 and ABIE40_RS28215 are separated by 77 nucleotides ABIE40_RS28215 and ABIE40_RS28220 overlap by 21 nucleotides ABIE40_RS28220 and ABIE40_RS28225 are separated by 384 nucleotides
ABIE40_RS28210: ABIE40_RS28210 - SDR family oxidoreductase, at 352,658 to 353,563
_RS28210
ABIE40_RS28215: ABIE40_RS28215 - TetR/AcrR family transcriptional regulator, at 353,641 to 354,264
_RS28215
ABIE40_RS28220: ABIE40_RS28220 - AraC family transcriptional regulator, at 354,244 to 354,849
_RS28220
ABIE40_RS28225: ABIE40_RS28225 - SDR family oxidoreductase, at 355,234 to 355,968
_RS28225
Position (kb) 354
355 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 353.438 kb on + strand, within ABIE40_RS28210 at 353.819 kb on - strand, within ABIE40_RS28215 at 353.819 kb on - strand, within ABIE40_RS28215 at 353.819 kb on - strand, within ABIE40_RS28215 at 353.821 kb on + strand, within ABIE40_RS28215 at 353.821 kb on + strand, within ABIE40_RS28215 at 353.822 kb on - strand, within ABIE40_RS28215 at 353.822 kb on - strand, within ABIE40_RS28215 at 354.040 kb on + strand, within ABIE40_RS28215 at 354.041 kb on - strand, within ABIE40_RS28215 at 354.041 kb on - strand, within ABIE40_RS28215 at 354.065 kb on - strand, within ABIE40_RS28215 at 354.321 kb on + strand, within ABIE40_RS28220 at 354.321 kb on + strand, within ABIE40_RS28220 at 354.321 kb on + strand, within ABIE40_RS28220 at 354.321 kb on + strand, within ABIE40_RS28220 at 354.322 kb on - strand, within ABIE40_RS28220 at 354.322 kb on - strand, within ABIE40_RS28220 at 354.322 kb on - strand, within ABIE40_RS28220 at 354.322 kb on - strand, within ABIE40_RS28220 at 354.322 kb on - strand, within ABIE40_RS28220 at 354.323 kb on + strand, within ABIE40_RS28220 at 354.323 kb on + strand, within ABIE40_RS28220 at 354.323 kb on + strand, within ABIE40_RS28220 at 354.323 kb on + strand, within ABIE40_RS28220 at 354.323 kb on + strand, within ABIE40_RS28220 at 354.324 kb on - strand, within ABIE40_RS28220 at 354.324 kb on - strand, within ABIE40_RS28220 at 354.371 kb on - strand, within ABIE40_RS28220 at 354.391 kb on - strand, within ABIE40_RS28220 at 354.410 kb on + strand, within ABIE40_RS28220 at 354.411 kb on - strand, within ABIE40_RS28220 at 354.411 kb on - strand, within ABIE40_RS28220 at 354.625 kb on + strand, within ABIE40_RS28220 at 354.625 kb on + strand, within ABIE40_RS28220 at 354.626 kb on - strand, within ABIE40_RS28220 at 354.626 kb on - strand, within ABIE40_RS28220 at 354.670 kb on + strand, within ABIE40_RS28220 at 354.682 kb on + strand, within ABIE40_RS28220 at 354.720 kb on + strand, within ABIE40_RS28220 at 354.810 kb on + strand at 354.810 kb on + strand at 354.810 kb on + strand at 354.811 kb on - strand at 354.811 kb on - strand at 354.868 kb on - strand at 354.919 kb on - strand at 354.919 kb on - strand at 355.160 kb on + strand at 355.173 kb on + strand at 355.174 kb on - strand at 355.174 kb on - strand at 355.177 kb on + strand at 355.178 kb on - strand at 355.178 kb on - strand at 355.196 kb on + strand at 355.196 kb on + strand at 355.197 kb on - strand at 355.283 kb on + strand at 355.283 kb on + strand at 355.284 kb on - strand at 355.508 kb on + strand, within ABIE40_RS28225 at 355.508 kb on + strand, within ABIE40_RS28225 at 355.509 kb on - strand, within ABIE40_RS28225 at 355.693 kb on + strand, within ABIE40_RS28225 at 355.693 kb on + strand, within ABIE40_RS28225 at 355.785 kb on + strand, within ABIE40_RS28225 at 355.785 kb on + strand, within ABIE40_RS28225 at 355.785 kb on + strand, within ABIE40_RS28225 at 355.786 kb on - strand, within ABIE40_RS28225
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with 3-octanone 5.3129 mM remove 353,438 + ABIE40_RS28210 0.86 +0.1 353,819 - ABIE40_RS28215 0.29 -1.0 353,819 - ABIE40_RS28215 0.29 -1.0 353,819 - ABIE40_RS28215 0.29 -2.2 353,821 + ABIE40_RS28215 0.29 -1.2 353,821 + ABIE40_RS28215 0.29 -0.5 353,822 - ABIE40_RS28215 0.29 +1.2 353,822 - ABIE40_RS28215 0.29 -0.0 354,040 + ABIE40_RS28215 0.64 -1.5 354,041 - ABIE40_RS28215 0.64 -2.8 354,041 - ABIE40_RS28215 0.64 -1.0 354,065 - ABIE40_RS28215 0.68 -2.7 354,321 + ABIE40_RS28220 0.13 -1.2 354,321 + ABIE40_RS28220 0.13 -1.8 354,321 + ABIE40_RS28220 0.13 +0.1 354,321 + ABIE40_RS28220 0.13 -1.7 354,322 - ABIE40_RS28220 0.13 -0.5 354,322 - ABIE40_RS28220 0.13 +2.2 354,322 - ABIE40_RS28220 0.13 +1.2 354,322 - ABIE40_RS28220 0.13 +0.6 354,322 - ABIE40_RS28220 0.13 -0.5 354,323 + ABIE40_RS28220 0.13 -1.1 354,323 + ABIE40_RS28220 0.13 +0.6 354,323 + ABIE40_RS28220 0.13 +0.3 354,323 + ABIE40_RS28220 0.13 -2.2 354,323 + ABIE40_RS28220 0.13 -1.4 354,324 - ABIE40_RS28220 0.13 +0.6 354,324 - ABIE40_RS28220 0.13 +2.2 354,371 - ABIE40_RS28220 0.21 -1.0 354,391 - ABIE40_RS28220 0.24 +2.2 354,410 + ABIE40_RS28220 0.27 +1.2 354,411 - ABIE40_RS28220 0.28 -0.4 354,411 - ABIE40_RS28220 0.28 +0.2 354,625 + ABIE40_RS28220 0.63 -2.2 354,625 + ABIE40_RS28220 0.63 +0.6 354,626 - ABIE40_RS28220 0.63 -0.1 354,626 - ABIE40_RS28220 0.63 +0.2 354,670 + ABIE40_RS28220 0.70 -0.0 354,682 + ABIE40_RS28220 0.72 +0.2 354,720 + ABIE40_RS28220 0.79 -1.4 354,810 + +3.2 354,810 + +0.9 354,810 + -0.4 354,811 - -1.2 354,811 - -0.2 354,868 - -1.0 354,919 - -0.3 354,919 - +0.2 355,160 + +1.8 355,173 + +0.6 355,174 - +0.1 355,174 - +1.0 355,177 + +0.7 355,178 - +0.5 355,178 - -2.8 355,196 + +1.2 355,196 + +0.2 355,197 - -0.5 355,283 + +0.4 355,283 + +1.5 355,284 - -0.1 355,508 + ABIE40_RS28225 0.37 -1.7 355,508 + ABIE40_RS28225 0.37 -1.1 355,509 - ABIE40_RS28225 0.37 +1.2 355,693 + ABIE40_RS28225 0.62 -2.2 355,693 + ABIE40_RS28225 0.62 +2.6 355,785 + ABIE40_RS28225 0.75 +0.1 355,785 + ABIE40_RS28225 0.75 +0.7 355,785 + ABIE40_RS28225 0.75 +1.6 355,786 - ABIE40_RS28225 0.75 -0.0
Or see this region's nucleotide sequence