Experiment: R2A with 3-octanone 5.3129 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE40_RS02395 and ABIE40_RS02400 are separated by 11 nucleotides ABIE40_RS02400 and ABIE40_RS02405 are separated by 104 nucleotides ABIE40_RS02405 and ABIE40_RS02410 are separated by 90 nucleotides ABIE40_RS02410 and ABIE40_RS02415 are separated by 116 nucleotides ABIE40_RS02415 and ABIE40_RS02420 overlap by 1 nucleotides
ABIE40_RS02395: ABIE40_RS02395 - thermonuclease family protein, at 475,869 to 476,606
_RS02395
ABIE40_RS02400: ABIE40_RS02400 - helix-turn-helix transcriptional regulator, at 476,618 to 477,283
_RS02400
ABIE40_RS02405: ABIE40_RS02405 - response regulator transcription factor, at 477,388 to 478,035
_RS02405
ABIE40_RS02410: ABIE40_RS02410 - hypothetical protein, at 478,126 to 478,524
_RS02410
ABIE40_RS02415: ABIE40_RS02415 - DUF952 domain-containing protein, at 478,641 to 478,991
_RS02415
ABIE40_RS02420: ABIE40_RS02420 - quinone-dependent dihydroorotate dehydrogenase, at 478,991 to 480,079
_RS02420
Position (kb) 477
478
479 Strain fitness (log2 ratio) -2
-1
0
1
2 at 476.471 kb on + strand, within ABIE40_RS02395 at 477.352 kb on - strand at 477.352 kb on - strand at 477.352 kb on - strand at 477.352 kb on - strand at 477.516 kb on - strand, within ABIE40_RS02405 at 477.642 kb on - strand, within ABIE40_RS02405 at 477.809 kb on + strand, within ABIE40_RS02405 at 477.810 kb on - strand, within ABIE40_RS02405 at 478.074 kb on + strand at 478.075 kb on - strand at 478.109 kb on + strand at 478.109 kb on + strand at 478.109 kb on + strand at 478.110 kb on - strand at 478.110 kb on - strand at 478.110 kb on - strand at 478.110 kb on - strand at 478.110 kb on - strand at 478.110 kb on - strand at 478.110 kb on - strand at 478.110 kb on - strand at 478.110 kb on - strand at 478.110 kb on - strand at 478.111 kb on + strand at 478.111 kb on + strand at 478.111 kb on + strand at 478.111 kb on + strand at 478.111 kb on + strand at 478.111 kb on + strand at 478.111 kb on + strand at 478.111 kb on + strand at 478.111 kb on + strand at 478.112 kb on - strand at 478.112 kb on - strand at 478.112 kb on - strand at 478.112 kb on - strand at 478.112 kb on - strand at 478.112 kb on - strand at 478.112 kb on - strand at 478.112 kb on - strand at 478.112 kb on - strand at 478.112 kb on - strand at 478.117 kb on + strand at 478.117 kb on + strand at 478.117 kb on + strand at 478.118 kb on - strand at 478.118 kb on - strand at 478.119 kb on + strand at 478.119 kb on + strand at 478.119 kb on + strand at 478.120 kb on - strand at 478.120 kb on - strand at 478.120 kb on - strand at 478.323 kb on - strand, within ABIE40_RS02410 at 478.662 kb on + strand at 478.662 kb on + strand at 478.794 kb on + strand, within ABIE40_RS02415 at 478.902 kb on + strand, within ABIE40_RS02415 at 478.970 kb on + strand at 478.985 kb on + strand at 478.985 kb on + strand at 478.985 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with 3-octanone 5.3129 mM remove 476,471 + ABIE40_RS02395 0.82 -1.1 477,352 - +0.5 477,352 - -1.1 477,352 - -0.8 477,352 - +2.0 477,516 - ABIE40_RS02405 0.20 -0.9 477,642 - ABIE40_RS02405 0.39 -1.1 477,809 + ABIE40_RS02405 0.65 -0.2 477,810 - ABIE40_RS02405 0.65 -0.7 478,074 + -0.4 478,075 - +0.0 478,109 + -1.7 478,109 + -0.8 478,109 + -1.9 478,110 - +1.4 478,110 - +1.8 478,110 - +0.3 478,110 - +0.4 478,110 - +0.4 478,110 - -0.2 478,110 - +1.2 478,110 - +0.6 478,110 - +0.7 478,110 - +0.8 478,111 + +0.8 478,111 + -0.5 478,111 + -1.4 478,111 + -1.0 478,111 + +0.2 478,111 + -1.2 478,111 + -0.1 478,111 + -0.0 478,111 + +0.4 478,112 - +0.5 478,112 - +0.6 478,112 - -1.0 478,112 - -0.3 478,112 - -0.0 478,112 - -0.4 478,112 - +1.1 478,112 - -1.1 478,112 - +0.2 478,112 - +1.0 478,117 + -0.8 478,117 + -0.5 478,117 + -0.3 478,118 - +0.2 478,118 - -0.7 478,119 + -1.3 478,119 + -0.5 478,119 + -1.2 478,120 - +0.2 478,120 - -1.9 478,120 - +0.6 478,323 - ABIE40_RS02410 0.49 -0.2 478,662 + -1.6 478,662 + +1.2 478,794 + ABIE40_RS02415 0.44 -0.2 478,902 + ABIE40_RS02415 0.74 -0.2 478,970 + +0.3 478,985 + -1.2 478,985 + +1.1 478,985 + -0.5
Or see this region's nucleotide sequence