Experiment: R2A with 3-octanone 5.3129 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE40_RS27675 and ABIE40_RS27680 are separated by 447 nucleotides ABIE40_RS27680 and ABIE40_RS27685 are separated by 250 nucleotides ABIE40_RS27685 and ABIE40_RS27690 are separated by 92 nucleotides
ABIE40_RS27675: ABIE40_RS27675 - hypothetical protein, at 247,475 to 247,714
_RS27675
ABIE40_RS27680: ABIE40_RS27680 - hypothetical protein, at 248,162 to 248,389
_RS27680
ABIE40_RS27685: ABIE40_RS27685 - hypothetical protein, at 248,640 to 248,927
_RS27685
ABIE40_RS27690: ABIE40_RS27690 - Hsp20 family protein, at 249,020 to 249,487
_RS27690
Position (kb) 248
249 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 247.162 kb on + strand at 247.201 kb on + strand at 247.202 kb on - strand at 247.214 kb on + strand at 247.214 kb on + strand at 247.214 kb on + strand at 247.214 kb on + strand at 247.215 kb on - strand at 247.215 kb on - strand at 247.260 kb on + strand at 247.260 kb on + strand at 247.261 kb on - strand at 247.264 kb on + strand at 247.264 kb on + strand at 247.264 kb on + strand at 247.264 kb on + strand at 247.264 kb on + strand at 247.264 kb on + strand at 247.265 kb on - strand at 247.265 kb on - strand at 247.271 kb on + strand at 247.386 kb on - strand at 247.386 kb on - strand at 247.438 kb on - strand at 247.438 kb on - strand at 247.521 kb on + strand, within ABIE40_RS27675 at 247.522 kb on - strand, within ABIE40_RS27675 at 247.522 kb on - strand, within ABIE40_RS27675 at 247.542 kb on + strand, within ABIE40_RS27675 at 247.542 kb on + strand, within ABIE40_RS27675 at 247.542 kb on + strand, within ABIE40_RS27675 at 247.543 kb on - strand, within ABIE40_RS27675 at 247.543 kb on - strand, within ABIE40_RS27675 at 247.566 kb on + strand, within ABIE40_RS27675 at 247.567 kb on - strand, within ABIE40_RS27675 at 247.567 kb on - strand, within ABIE40_RS27675 at 247.581 kb on + strand, within ABIE40_RS27675 at 247.695 kb on + strand at 247.704 kb on + strand at 247.705 kb on - strand at 247.705 kb on - strand at 247.708 kb on + strand at 247.708 kb on + strand at 247.708 kb on + strand at 247.708 kb on + strand at 247.709 kb on - strand at 247.743 kb on + strand at 247.744 kb on - strand at 247.882 kb on + strand at 247.882 kb on + strand at 247.883 kb on - strand at 247.885 kb on + strand at 247.886 kb on - strand at 247.896 kb on + strand at 247.896 kb on + strand at 247.896 kb on + strand at 247.897 kb on - strand at 247.913 kb on - strand at 247.927 kb on + strand at 247.927 kb on + strand at 247.954 kb on + strand at 247.955 kb on - strand at 248.012 kb on + strand at 248.012 kb on + strand at 248.012 kb on + strand at 248.012 kb on + strand at 248.013 kb on - strand at 248.013 kb on - strand at 248.013 kb on - strand at 248.013 kb on - strand at 248.013 kb on - strand at 248.013 kb on - strand at 248.072 kb on + strand at 248.073 kb on - strand at 248.073 kb on - strand at 248.073 kb on - strand at 248.099 kb on + strand at 248.100 kb on - strand at 248.148 kb on + strand at 248.245 kb on + strand, within ABIE40_RS27680 at 248.246 kb on - strand, within ABIE40_RS27680 at 248.246 kb on - strand, within ABIE40_RS27680 at 248.527 kb on + strand at 248.546 kb on - strand at 248.546 kb on - strand at 248.627 kb on - strand at 248.666 kb on + strand at 248.667 kb on - strand at 248.667 kb on - strand at 248.697 kb on - strand, within ABIE40_RS27685 at 248.817 kb on - strand, within ABIE40_RS27685 at 248.977 kb on + strand at 248.978 kb on - strand at 248.978 kb on - strand at 248.979 kb on + strand at 248.980 kb on - strand at 249.009 kb on + strand at 249.022 kb on - strand at 249.022 kb on - strand at 249.118 kb on + strand, within ABIE40_RS27690 at 249.118 kb on + strand, within ABIE40_RS27690 at 249.170 kb on + strand, within ABIE40_RS27690 at 249.171 kb on - strand, within ABIE40_RS27690 at 249.171 kb on - strand, within ABIE40_RS27690 at 249.171 kb on - strand, within ABIE40_RS27690 at 249.227 kb on + strand, within ABIE40_RS27690 at 249.292 kb on + strand, within ABIE40_RS27690 at 249.335 kb on + strand, within ABIE40_RS27690 at 249.343 kb on - strand, within ABIE40_RS27690 at 249.372 kb on + strand, within ABIE40_RS27690 at 249.373 kb on - strand, within ABIE40_RS27690
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with 3-octanone 5.3129 mM remove 247,162 + +1.0 247,201 + -0.3 247,202 - -1.0 247,214 + +0.4 247,214 + -1.4 247,214 + +1.9 247,214 + -0.7 247,215 - +2.2 247,215 - +2.2 247,260 + -0.8 247,260 + +1.0 247,261 - +0.6 247,264 + -0.1 247,264 + +0.6 247,264 + -0.1 247,264 + +2.2 247,264 + -0.1 247,264 + +1.2 247,265 - +1.2 247,265 - +0.6 247,271 + -1.1 247,386 - +0.5 247,386 - +2.6 247,438 - -0.4 247,438 - -1.7 247,521 + ABIE40_RS27675 0.19 +1.8 247,522 - ABIE40_RS27675 0.20 -2.5 247,522 - ABIE40_RS27675 0.20 +0.2 247,542 + ABIE40_RS27675 0.28 -3.4 247,542 + ABIE40_RS27675 0.28 +2.2 247,542 + ABIE40_RS27675 0.28 -1.2 247,543 - ABIE40_RS27675 0.28 -0.1 247,543 - ABIE40_RS27675 0.28 +0.2 247,566 + ABIE40_RS27675 0.38 +2.2 247,567 - ABIE40_RS27675 0.38 -1.6 247,567 - ABIE40_RS27675 0.38 +0.2 247,581 + ABIE40_RS27675 0.44 -0.7 247,695 + -0.4 247,704 + -1.3 247,705 - -1.6 247,705 - +1.2 247,708 + -1.3 247,708 + +1.5 247,708 + -1.9 247,708 + +0.6 247,709 - -0.4 247,743 + +0.7 247,744 - -1.5 247,882 + +0.6 247,882 + -1.0 247,883 - -1.1 247,885 + -1.1 247,886 - -1.4 247,896 + -0.3 247,896 + +1.2 247,896 + -0.1 247,897 - -1.1 247,913 - +0.6 247,927 + -0.4 247,927 + -0.7 247,954 + -0.7 247,955 - -0.4 248,012 + +3.2 248,012 + -1.9 248,012 + -0.2 248,012 + +0.4 248,013 - +0.8 248,013 - -0.6 248,013 - -2.3 248,013 - -0.3 248,013 - +2.2 248,013 - +2.5 248,072 + -0.5 248,073 - +0.4 248,073 - -0.6 248,073 - -0.4 248,099 + +2.8 248,100 - +2.8 248,148 + -0.2 248,245 + ABIE40_RS27680 0.36 -1.4 248,246 - ABIE40_RS27680 0.37 +1.2 248,246 - ABIE40_RS27680 0.37 -2.5 248,527 + -0.8 248,546 - -1.0 248,546 - +1.5 248,627 - -0.9 248,666 + -1.0 248,667 - -1.2 248,667 - +1.4 248,697 - ABIE40_RS27685 0.20 +0.8 248,817 - ABIE40_RS27685 0.61 +2.2 248,977 + -0.3 248,978 - +1.0 248,978 - +0.2 248,979 + +2.2 248,980 - +0.8 249,009 + +1.2 249,022 - -0.4 249,022 - +0.2 249,118 + ABIE40_RS27690 0.21 -1.0 249,118 + ABIE40_RS27690 0.21 +2.2 249,170 + ABIE40_RS27690 0.32 -1.3 249,171 - ABIE40_RS27690 0.32 +1.2 249,171 - ABIE40_RS27690 0.32 -1.4 249,171 - ABIE40_RS27690 0.32 +0.9 249,227 + ABIE40_RS27690 0.44 -0.4 249,292 + ABIE40_RS27690 0.58 +0.5 249,335 + ABIE40_RS27690 0.67 -1.5 249,343 - ABIE40_RS27690 0.69 +0.7 249,372 + ABIE40_RS27690 0.75 +2.2 249,373 - ABIE40_RS27690 0.75 +1.2
Or see this region's nucleotide sequence