Experiment: R2A with 3-octanone 5.3129 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE40_RS27325 and ABIE40_RS27330 are separated by 104 nucleotides ABIE40_RS27330 and ABIE40_RS27335 are separated by 4 nucleotides ABIE40_RS27335 and ABIE40_RS27340 are separated by 141 nucleotides
ABIE40_RS27325: ABIE40_RS27325 - hypothetical protein, at 180,188 to 180,544
_RS27325
ABIE40_RS27330: ABIE40_RS27330 - alpha/beta hydrolase, at 180,649 to 181,518
_RS27330
ABIE40_RS27335: ABIE40_RS27335 - hypothetical protein, at 181,523 to 181,732
_RS27335
ABIE40_RS27340: ABIE40_RS27340 - pyridoxamine 5'-phosphate oxidase family protein, at 181,874 to 182,320
_RS27340
Position (kb) 181
182 Strain fitness (log2 ratio) -2
-1
0
1
2 at 180.538 kb on + strand at 180.538 kb on + strand at 180.538 kb on + strand at 180.538 kb on + strand at 180.539 kb on - strand at 180.539 kb on - strand at 180.539 kb on - strand at 180.545 kb on - strand at 180.545 kb on - strand at 180.610 kb on + strand at 180.611 kb on - strand at 180.770 kb on - strand, within ABIE40_RS27330 at 180.788 kb on + strand, within ABIE40_RS27330 at 180.872 kb on - strand, within ABIE40_RS27330 at 180.880 kb on - strand, within ABIE40_RS27330 at 180.904 kb on - strand, within ABIE40_RS27330 at 180.954 kb on + strand, within ABIE40_RS27330 at 180.965 kb on + strand, within ABIE40_RS27330 at 180.982 kb on + strand, within ABIE40_RS27330 at 180.983 kb on - strand, within ABIE40_RS27330 at 180.983 kb on - strand, within ABIE40_RS27330 at 180.983 kb on - strand, within ABIE40_RS27330 at 180.983 kb on - strand, within ABIE40_RS27330 at 181.073 kb on + strand, within ABIE40_RS27330 at 181.242 kb on + strand, within ABIE40_RS27330 at 181.266 kb on + strand, within ABIE40_RS27330 at 181.319 kb on + strand, within ABIE40_RS27330 at 181.319 kb on + strand, within ABIE40_RS27330 at 181.319 kb on + strand, within ABIE40_RS27330 at 181.320 kb on - strand, within ABIE40_RS27330 at 181.320 kb on - strand, within ABIE40_RS27330 at 181.486 kb on + strand at 181.487 kb on - strand at 181.625 kb on - strand, within ABIE40_RS27335 at 181.625 kb on - strand, within ABIE40_RS27335 at 181.658 kb on - strand, within ABIE40_RS27335 at 181.838 kb on + strand at 181.839 kb on - strand at 181.839 kb on - strand at 181.842 kb on + strand at 181.842 kb on + strand at 181.842 kb on + strand at 181.842 kb on + strand at 181.843 kb on - strand at 181.843 kb on - strand at 181.843 kb on - strand at 181.844 kb on + strand at 181.844 kb on + strand at 181.845 kb on - strand at 181.845 kb on - strand at 181.981 kb on + strand, within ABIE40_RS27340 at 182.001 kb on - strand, within ABIE40_RS27340 at 182.001 kb on - strand, within ABIE40_RS27340 at 182.027 kb on + strand, within ABIE40_RS27340 at 182.027 kb on + strand, within ABIE40_RS27340 at 182.028 kb on - strand, within ABIE40_RS27340 at 182.065 kb on - strand, within ABIE40_RS27340 at 182.065 kb on - strand, within ABIE40_RS27340 at 182.244 kb on + strand, within ABIE40_RS27340 at 182.244 kb on + strand, within ABIE40_RS27340 at 182.244 kb on + strand, within ABIE40_RS27340 at 182.245 kb on - strand, within ABIE40_RS27340 at 182.245 kb on - strand, within ABIE40_RS27340 at 182.264 kb on - strand, within ABIE40_RS27340 at 182.271 kb on - strand, within ABIE40_RS27340 at 182.301 kb on + strand at 182.302 kb on - strand at 182.319 kb on - strand at 182.319 kb on - strand at 182.341 kb on + strand at 182.341 kb on + strand at 182.342 kb on - strand at 182.342 kb on - strand at 182.410 kb on + strand at 182.410 kb on + strand at 182.540 kb on + strand at 182.540 kb on + strand at 182.540 kb on + strand at 182.541 kb on - strand at 182.635 kb on - strand at 182.659 kb on + strand at 182.714 kb on - strand at 182.727 kb on + strand at 182.728 kb on - strand at 182.728 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with 3-octanone 5.3129 mM remove 180,538 + -0.6 180,538 + +1.0 180,538 + +0.3 180,538 + -2.3 180,539 - +1.0 180,539 - +1.7 180,539 - +0.2 180,545 - +0.7 180,545 - +0.7 180,610 + -0.5 180,611 - +0.3 180,770 - ABIE40_RS27330 0.14 -0.6 180,788 + ABIE40_RS27330 0.16 +1.8 180,872 - ABIE40_RS27330 0.26 +0.1 180,880 - ABIE40_RS27330 0.27 +0.2 180,904 - ABIE40_RS27330 0.29 +2.6 180,954 + ABIE40_RS27330 0.35 -0.1 180,965 + ABIE40_RS27330 0.36 +0.2 180,982 + ABIE40_RS27330 0.38 +0.3 180,983 - ABIE40_RS27330 0.38 -0.1 180,983 - ABIE40_RS27330 0.38 -2.0 180,983 - ABIE40_RS27330 0.38 +0.2 180,983 - ABIE40_RS27330 0.38 +1.2 181,073 + ABIE40_RS27330 0.49 +1.5 181,242 + ABIE40_RS27330 0.68 +0.7 181,266 + ABIE40_RS27330 0.71 -0.6 181,319 + ABIE40_RS27330 0.77 -1.2 181,319 + ABIE40_RS27330 0.77 +2.2 181,319 + ABIE40_RS27330 0.77 +2.2 181,320 - ABIE40_RS27330 0.77 -1.5 181,320 - ABIE40_RS27330 0.77 -0.1 181,486 + -1.2 181,487 - +1.6 181,625 - ABIE40_RS27335 0.49 -0.1 181,625 - ABIE40_RS27335 0.49 -0.1 181,658 - ABIE40_RS27335 0.64 -1.3 181,838 + +2.1 181,839 - -0.8 181,839 - -1.6 181,842 + +0.7 181,842 + -1.5 181,842 + +1.5 181,842 + -0.7 181,843 - +2.2 181,843 - +0.9 181,843 - +0.2 181,844 + -1.2 181,844 + +2.7 181,845 - -0.1 181,845 - -0.6 181,981 + ABIE40_RS27340 0.24 -0.5 182,001 - ABIE40_RS27340 0.28 +2.2 182,001 - ABIE40_RS27340 0.28 -2.4 182,027 + ABIE40_RS27340 0.34 +0.5 182,027 + ABIE40_RS27340 0.34 +0.9 182,028 - ABIE40_RS27340 0.34 +1.2 182,065 - ABIE40_RS27340 0.43 -1.6 182,065 - ABIE40_RS27340 0.43 +0.7 182,244 + ABIE40_RS27340 0.83 -1.0 182,244 + ABIE40_RS27340 0.83 -0.6 182,244 + ABIE40_RS27340 0.83 -0.1 182,245 - ABIE40_RS27340 0.83 +1.2 182,245 - ABIE40_RS27340 0.83 +0.4 182,264 - ABIE40_RS27340 0.87 +0.7 182,271 - ABIE40_RS27340 0.89 -0.7 182,301 + +0.9 182,302 - -1.0 182,319 - +0.7 182,319 - -0.7 182,341 + +0.2 182,341 + +1.7 182,342 - +0.9 182,342 - +0.4 182,410 + -0.5 182,410 + +1.8 182,540 + -0.6 182,540 + -1.2 182,540 + +0.9 182,541 - +2.2 182,635 - -1.8 182,659 + -0.6 182,714 - -0.9 182,727 + -0.5 182,728 - -0.7 182,728 - +1.2
Or see this region's nucleotide sequence