Experiment: R2A with 3-octanone 5.3129 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE40_RS25510 and ABIE40_RS25515 are separated by 83 nucleotides ABIE40_RS25515 and ABIE40_RS25520 overlap by 4 nucleotides ABIE40_RS25520 and ABIE40_RS25525 are separated by 27 nucleotides
ABIE40_RS25510: ABIE40_RS25510 - sugar ABC transporter substrate-binding protein, at 1,276,601 to 1,277,839
_RS25510
ABIE40_RS25515: ABIE40_RS25515 - sugar ABC transporter permease, at 1,277,923 to 1,278,840
_RS25515
ABIE40_RS25520: ABIE40_RS25520 - carbohydrate ABC transporter permease, at 1,278,837 to 1,279,700
_RS25520
ABIE40_RS25525: ABIE40_RS25525 - glycoside hydrolase family 127 protein, at 1,279,728 to 1,281,656
_RS25525
Position (kb)
1277
1278
1279 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1276.923 kb on - strand, within ABIE40_RS25510 at 1276.964 kb on + strand, within ABIE40_RS25510 at 1276.964 kb on + strand, within ABIE40_RS25510 at 1276.979 kb on + strand, within ABIE40_RS25510 at 1276.979 kb on + strand, within ABIE40_RS25510 at 1276.979 kb on + strand, within ABIE40_RS25510 at 1276.979 kb on + strand, within ABIE40_RS25510 at 1276.979 kb on + strand, within ABIE40_RS25510 at 1277.033 kb on + strand, within ABIE40_RS25510 at 1277.033 kb on + strand, within ABIE40_RS25510 at 1277.033 kb on + strand, within ABIE40_RS25510 at 1277.033 kb on + strand, within ABIE40_RS25510 at 1277.034 kb on - strand, within ABIE40_RS25510 at 1277.129 kb on + strand, within ABIE40_RS25510 at 1277.129 kb on + strand, within ABIE40_RS25510 at 1277.129 kb on + strand, within ABIE40_RS25510 at 1277.130 kb on - strand, within ABIE40_RS25510 at 1277.130 kb on - strand, within ABIE40_RS25510 at 1277.130 kb on - strand, within ABIE40_RS25510 at 1277.130 kb on - strand, within ABIE40_RS25510 at 1277.138 kb on + strand, within ABIE40_RS25510 at 1277.139 kb on - strand, within ABIE40_RS25510 at 1277.213 kb on - strand, within ABIE40_RS25510 at 1277.248 kb on + strand, within ABIE40_RS25510 at 1277.279 kb on + strand, within ABIE40_RS25510 at 1277.279 kb on + strand, within ABIE40_RS25510 at 1277.280 kb on - strand, within ABIE40_RS25510 at 1277.280 kb on - strand, within ABIE40_RS25510 at 1277.280 kb on - strand, within ABIE40_RS25510 at 1277.492 kb on - strand, within ABIE40_RS25510 at 1277.549 kb on + strand, within ABIE40_RS25510 at 1277.549 kb on + strand, within ABIE40_RS25510 at 1277.549 kb on + strand, within ABIE40_RS25510 at 1277.550 kb on - strand, within ABIE40_RS25510 at 1277.550 kb on - strand, within ABIE40_RS25510 at 1277.550 kb on - strand, within ABIE40_RS25510 at 1277.550 kb on - strand, within ABIE40_RS25510 at 1277.551 kb on + strand, within ABIE40_RS25510 at 1277.551 kb on + strand, within ABIE40_RS25510 at 1277.551 kb on + strand, within ABIE40_RS25510 at 1277.551 kb on + strand, within ABIE40_RS25510 at 1277.551 kb on + strand, within ABIE40_RS25510 at 1277.551 kb on + strand, within ABIE40_RS25510 at 1277.551 kb on + strand, within ABIE40_RS25510 at 1277.551 kb on + strand, within ABIE40_RS25510 at 1277.551 kb on + strand, within ABIE40_RS25510 at 1277.552 kb on - strand, within ABIE40_RS25510 at 1277.552 kb on - strand, within ABIE40_RS25510 at 1277.552 kb on - strand, within ABIE40_RS25510 at 1277.552 kb on - strand, within ABIE40_RS25510 at 1277.552 kb on - strand, within ABIE40_RS25510 at 1277.555 kb on + strand, within ABIE40_RS25510 at 1277.555 kb on + strand, within ABIE40_RS25510 at 1277.555 kb on + strand, within ABIE40_RS25510 at 1277.556 kb on - strand, within ABIE40_RS25510 at 1277.588 kb on + strand, within ABIE40_RS25510 at 1277.588 kb on + strand, within ABIE40_RS25510 at 1277.588 kb on + strand, within ABIE40_RS25510 at 1277.589 kb on - strand, within ABIE40_RS25510 at 1277.589 kb on - strand, within ABIE40_RS25510 at 1277.693 kb on + strand, within ABIE40_RS25510 at 1277.695 kb on + strand, within ABIE40_RS25510 at 1277.695 kb on + strand, within ABIE40_RS25510 at 1277.695 kb on + strand, within ABIE40_RS25510 at 1277.695 kb on + strand, within ABIE40_RS25510 at 1277.696 kb on - strand, within ABIE40_RS25510 at 1277.696 kb on - strand, within ABIE40_RS25510 at 1277.837 kb on + strand at 1277.837 kb on + strand at 1277.998 kb on + strand at 1277.998 kb on + strand at 1277.999 kb on - strand at 1277.999 kb on - strand at 1277.999 kb on - strand at 1277.999 kb on - strand at 1277.999 kb on - strand at 1278.075 kb on + strand, within ABIE40_RS25515 at 1278.075 kb on + strand, within ABIE40_RS25515 at 1278.075 kb on + strand, within ABIE40_RS25515 at 1278.121 kb on + strand, within ABIE40_RS25515 at 1278.121 kb on + strand, within ABIE40_RS25515 at 1278.121 kb on + strand, within ABIE40_RS25515 at 1278.121 kb on + strand, within ABIE40_RS25515 at 1278.122 kb on - strand, within ABIE40_RS25515 at 1278.122 kb on - strand, within ABIE40_RS25515 at 1278.122 kb on - strand, within ABIE40_RS25515 at 1278.122 kb on - strand, within ABIE40_RS25515 at 1278.178 kb on + strand, within ABIE40_RS25515 at 1278.178 kb on + strand, within ABIE40_RS25515 at 1278.179 kb on - strand, within ABIE40_RS25515 at 1278.179 kb on - strand, within ABIE40_RS25515 at 1278.179 kb on - strand, within ABIE40_RS25515 at 1278.265 kb on + strand, within ABIE40_RS25515 at 1278.265 kb on + strand, within ABIE40_RS25515 at 1278.265 kb on + strand, within ABIE40_RS25515 at 1278.266 kb on - strand, within ABIE40_RS25515 at 1278.266 kb on - strand, within ABIE40_RS25515 at 1278.266 kb on - strand, within ABIE40_RS25515 at 1278.403 kb on - strand, within ABIE40_RS25515 at 1278.655 kb on + strand, within ABIE40_RS25515 at 1278.655 kb on + strand, within ABIE40_RS25515 at 1278.656 kb on - strand, within ABIE40_RS25515 at 1278.703 kb on + strand, within ABIE40_RS25515 at 1278.703 kb on + strand, within ABIE40_RS25515 at 1278.703 kb on + strand, within ABIE40_RS25515 at 1278.703 kb on + strand, within ABIE40_RS25515 at 1278.704 kb on - strand, within ABIE40_RS25515 at 1278.704 kb on - strand, within ABIE40_RS25515 at 1278.704 kb on - strand, within ABIE40_RS25515 at 1278.706 kb on + strand, within ABIE40_RS25515 at 1278.706 kb on + strand, within ABIE40_RS25515 at 1278.706 kb on + strand, within ABIE40_RS25515 at 1278.706 kb on + strand, within ABIE40_RS25515 at 1278.706 kb on + strand, within ABIE40_RS25515 at 1278.707 kb on + strand, within ABIE40_RS25515 at 1278.707 kb on - strand, within ABIE40_RS25515 at 1278.707 kb on - strand, within ABIE40_RS25515 at 1278.707 kb on - strand, within ABIE40_RS25515 at 1278.707 kb on - strand, within ABIE40_RS25515 at 1278.746 kb on - strand, within ABIE40_RS25515 at 1278.818 kb on - strand at 1278.891 kb on + strand at 1278.892 kb on - strand at 1279.032 kb on + strand, within ABIE40_RS25520 at 1279.032 kb on + strand, within ABIE40_RS25520 at 1279.032 kb on + strand, within ABIE40_RS25520 at 1279.032 kb on + strand, within ABIE40_RS25520 at 1279.032 kb on + strand, within ABIE40_RS25520 at 1279.032 kb on + strand, within ABIE40_RS25520 at 1279.032 kb on + strand, within ABIE40_RS25520 at 1279.033 kb on - strand, within ABIE40_RS25520 at 1279.033 kb on - strand, within ABIE40_RS25520 at 1279.033 kb on - strand, within ABIE40_RS25520 at 1279.047 kb on - strand, within ABIE40_RS25520 at 1279.047 kb on - strand, within ABIE40_RS25520 at 1279.299 kb on + strand, within ABIE40_RS25520 at 1279.299 kb on + strand, within ABIE40_RS25520 at 1279.299 kb on + strand, within ABIE40_RS25520 at 1279.533 kb on + strand, within ABIE40_RS25520 at 1279.533 kb on + strand, within ABIE40_RS25520 at 1279.533 kb on + strand, within ABIE40_RS25520 at 1279.533 kb on + strand, within ABIE40_RS25520 at 1279.534 kb on - strand, within ABIE40_RS25520 at 1279.534 kb on - strand, within ABIE40_RS25520 at 1279.534 kb on - strand, within ABIE40_RS25520 at 1279.662 kb on + strand at 1279.662 kb on + strand at 1279.662 kb on + strand at 1279.663 kb on - strand at 1279.665 kb on + strand at 1279.665 kb on + strand at 1279.665 kb on + strand at 1279.665 kb on + strand at 1279.666 kb on - strand at 1279.666 kb on - strand at 1279.666 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with 3-octanone 5.3129 mM remove 1,276,923 - ABIE40_RS25510 0.26 -1.6 1,276,964 + ABIE40_RS25510 0.29 -0.7 1,276,964 + ABIE40_RS25510 0.29 +0.3 1,276,979 + ABIE40_RS25510 0.31 -1.0 1,276,979 + ABIE40_RS25510 0.31 +1.1 1,276,979 + ABIE40_RS25510 0.31 -1.2 1,276,979 + ABIE40_RS25510 0.31 +0.5 1,276,979 + ABIE40_RS25510 0.31 -1.8 1,277,033 + ABIE40_RS25510 0.35 -1.7 1,277,033 + ABIE40_RS25510 0.35 -0.1 1,277,033 + ABIE40_RS25510 0.35 +0.0 1,277,033 + ABIE40_RS25510 0.35 -0.1 1,277,034 - ABIE40_RS25510 0.35 -0.8 1,277,129 + ABIE40_RS25510 0.43 -0.1 1,277,129 + ABIE40_RS25510 0.43 +0.7 1,277,129 + ABIE40_RS25510 0.43 -1.1 1,277,130 - ABIE40_RS25510 0.43 +0.4 1,277,130 - ABIE40_RS25510 0.43 -0.5 1,277,130 - ABIE40_RS25510 0.43 -2.3 1,277,130 - ABIE40_RS25510 0.43 +0.3 1,277,138 + ABIE40_RS25510 0.43 -0.2 1,277,139 - ABIE40_RS25510 0.43 -1.2 1,277,213 - ABIE40_RS25510 0.49 -1.8 1,277,248 + ABIE40_RS25510 0.52 -0.3 1,277,279 + ABIE40_RS25510 0.55 +0.1 1,277,279 + ABIE40_RS25510 0.55 -0.3 1,277,280 - ABIE40_RS25510 0.55 -0.2 1,277,280 - ABIE40_RS25510 0.55 +0.3 1,277,280 - ABIE40_RS25510 0.55 +0.2 1,277,492 - ABIE40_RS25510 0.72 -1.2 1,277,549 + ABIE40_RS25510 0.77 -0.6 1,277,549 + ABIE40_RS25510 0.77 -2.0 1,277,549 + ABIE40_RS25510 0.77 +0.5 1,277,550 - ABIE40_RS25510 0.77 -1.9 1,277,550 - ABIE40_RS25510 0.77 -0.3 1,277,550 - ABIE40_RS25510 0.77 +1.3 1,277,550 - ABIE40_RS25510 0.77 +1.6 1,277,551 + ABIE40_RS25510 0.77 -0.9 1,277,551 + ABIE40_RS25510 0.77 +1.1 1,277,551 + ABIE40_RS25510 0.77 +0.2 1,277,551 + ABIE40_RS25510 0.77 +2.3 1,277,551 + ABIE40_RS25510 0.77 +0.3 1,277,551 + ABIE40_RS25510 0.77 -1.6 1,277,551 + ABIE40_RS25510 0.77 -0.3 1,277,551 + ABIE40_RS25510 0.77 -2.4 1,277,551 + ABIE40_RS25510 0.77 -0.7 1,277,552 - ABIE40_RS25510 0.77 +0.7 1,277,552 - ABIE40_RS25510 0.77 -0.6 1,277,552 - ABIE40_RS25510 0.77 -2.1 1,277,552 - ABIE40_RS25510 0.77 +2.7 1,277,552 - ABIE40_RS25510 0.77 -0.5 1,277,555 + ABIE40_RS25510 0.77 +0.7 1,277,555 + ABIE40_RS25510 0.77 -0.5 1,277,555 + ABIE40_RS25510 0.77 -0.2 1,277,556 - ABIE40_RS25510 0.77 +0.6 1,277,588 + ABIE40_RS25510 0.80 +0.1 1,277,588 + ABIE40_RS25510 0.80 +1.4 1,277,588 + ABIE40_RS25510 0.80 +1.4 1,277,589 - ABIE40_RS25510 0.80 +0.2 1,277,589 - ABIE40_RS25510 0.80 +1.5 1,277,693 + ABIE40_RS25510 0.88 -0.7 1,277,695 + ABIE40_RS25510 0.88 -0.4 1,277,695 + ABIE40_RS25510 0.88 -0.0 1,277,695 + ABIE40_RS25510 0.88 +0.2 1,277,695 + ABIE40_RS25510 0.88 +1.3 1,277,696 - ABIE40_RS25510 0.88 +0.5 1,277,696 - ABIE40_RS25510 0.88 -2.1 1,277,837 + +0.5 1,277,837 + -0.9 1,277,998 + +0.1 1,277,998 + +0.3 1,277,999 - +1.0 1,277,999 - +0.0 1,277,999 - +2.1 1,277,999 - -0.2 1,277,999 - -0.7 1,278,075 + ABIE40_RS25515 0.17 -1.7 1,278,075 + ABIE40_RS25515 0.17 +1.1 1,278,075 + ABIE40_RS25515 0.17 -0.7 1,278,121 + ABIE40_RS25515 0.22 -0.9 1,278,121 + ABIE40_RS25515 0.22 -2.8 1,278,121 + ABIE40_RS25515 0.22 -3.0 1,278,121 + ABIE40_RS25515 0.22 -1.7 1,278,122 - ABIE40_RS25515 0.22 -2.7 1,278,122 - ABIE40_RS25515 0.22 +0.2 1,278,122 - ABIE40_RS25515 0.22 -0.4 1,278,122 - ABIE40_RS25515 0.22 -0.2 1,278,178 + ABIE40_RS25515 0.28 -0.2 1,278,178 + ABIE40_RS25515 0.28 -2.1 1,278,179 - ABIE40_RS25515 0.28 -1.2 1,278,179 - ABIE40_RS25515 0.28 -0.2 1,278,179 - ABIE40_RS25515 0.28 +0.0 1,278,265 + ABIE40_RS25515 0.37 +2.1 1,278,265 + ABIE40_RS25515 0.37 -1.1 1,278,265 + ABIE40_RS25515 0.37 -1.4 1,278,266 - ABIE40_RS25515 0.37 -0.9 1,278,266 - ABIE40_RS25515 0.37 -0.3 1,278,266 - ABIE40_RS25515 0.37 -0.7 1,278,403 - ABIE40_RS25515 0.52 -0.0 1,278,655 + ABIE40_RS25515 0.80 -1.4 1,278,655 + ABIE40_RS25515 0.80 -1.1 1,278,656 - ABIE40_RS25515 0.80 -1.0 1,278,703 + ABIE40_RS25515 0.85 +1.1 1,278,703 + ABIE40_RS25515 0.85 -0.2 1,278,703 + ABIE40_RS25515 0.85 -0.5 1,278,703 + ABIE40_RS25515 0.85 +0.5 1,278,704 - ABIE40_RS25515 0.85 -2.0 1,278,704 - ABIE40_RS25515 0.85 -2.1 1,278,704 - ABIE40_RS25515 0.85 -2.3 1,278,706 + ABIE40_RS25515 0.85 +0.1 1,278,706 + ABIE40_RS25515 0.85 -0.2 1,278,706 + ABIE40_RS25515 0.85 +0.2 1,278,706 + ABIE40_RS25515 0.85 +0.1 1,278,706 + ABIE40_RS25515 0.85 -0.5 1,278,707 + ABIE40_RS25515 0.85 +0.5 1,278,707 - ABIE40_RS25515 0.85 -2.6 1,278,707 - ABIE40_RS25515 0.85 +1.2 1,278,707 - ABIE40_RS25515 0.85 -1.9 1,278,707 - ABIE40_RS25515 0.85 -0.3 1,278,746 - ABIE40_RS25515 0.90 +0.1 1,278,818 - -0.0 1,278,891 + -0.4 1,278,892 - -0.8 1,279,032 + ABIE40_RS25520 0.23 +0.4 1,279,032 + ABIE40_RS25520 0.23 -0.8 1,279,032 + ABIE40_RS25520 0.23 -0.1 1,279,032 + ABIE40_RS25520 0.23 -0.9 1,279,032 + ABIE40_RS25520 0.23 +0.8 1,279,032 + ABIE40_RS25520 0.23 -1.3 1,279,032 + ABIE40_RS25520 0.23 +0.0 1,279,033 - ABIE40_RS25520 0.23 -1.5 1,279,033 - ABIE40_RS25520 0.23 -0.0 1,279,033 - ABIE40_RS25520 0.23 -0.1 1,279,047 - ABIE40_RS25520 0.24 +0.5 1,279,047 - ABIE40_RS25520 0.24 +0.6 1,279,299 + ABIE40_RS25520 0.53 +0.2 1,279,299 + ABIE40_RS25520 0.53 -0.2 1,279,299 + ABIE40_RS25520 0.53 +0.2 1,279,533 + ABIE40_RS25520 0.81 -0.8 1,279,533 + ABIE40_RS25520 0.81 +1.2 1,279,533 + ABIE40_RS25520 0.81 -0.6 1,279,533 + ABIE40_RS25520 0.81 +1.4 1,279,534 - ABIE40_RS25520 0.81 -0.2 1,279,534 - ABIE40_RS25520 0.81 -0.7 1,279,534 - ABIE40_RS25520 0.81 -2.4 1,279,662 + -0.5 1,279,662 + -0.7 1,279,662 + +1.5 1,279,663 - +1.5 1,279,665 + +0.5 1,279,665 + +1.1 1,279,665 + -0.3 1,279,665 + -1.5 1,279,666 - -1.1 1,279,666 - -0.9 1,279,666 - -1.2
Or see this region's nucleotide sequence