Experiment: R2A with 3-octanone 5.3129 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE40_RS10225 and ABIE40_RS10230 are separated by 30 nucleotides ABIE40_RS10230 and ABIE40_RS10235 are separated by 59 nucleotides ABIE40_RS10235 and ABIE40_RS10240 are separated by 69 nucleotides ABIE40_RS10240 and ABIE40_RS10245 are separated by 198 nucleotides
ABIE40_RS10225: ABIE40_RS10225 - inositol monophosphatase family protein, at 1,967,158 to 1,967,982
_RS10225
ABIE40_RS10230: ABIE40_RS10230 - tRNA 2-thiocytidine(32) synthetase TtcA, at 1,968,013 to 1,968,897
_RS10230
ABIE40_RS10235: ABIE40_RS10235 - glutaminase, at 1,968,957 to 1,969,886
_RS10235
ABIE40_RS10240: ABIE40_RS10240 - 30S ribosomal protein S4, at 1,969,956 to 1,970,573
_RS10240
ABIE40_RS10245: ABIE40_RS10245 - MarR family transcriptional regulator, at 1,970,772 to 1,971,299
_RS10245
Position (kb)
1968
1969
1970 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1967.962 kb on + strand at 1967.962 kb on + strand at 1967.963 kb on - strand at 1968.014 kb on + strand at 1968.014 kb on + strand at 1968.014 kb on + strand at 1968.015 kb on - strand at 1968.015 kb on - strand at 1968.121 kb on - strand, within ABIE40_RS10230 at 1968.121 kb on - strand, within ABIE40_RS10230 at 1968.121 kb on - strand, within ABIE40_RS10230 at 1968.121 kb on - strand, within ABIE40_RS10230 at 1968.162 kb on + strand, within ABIE40_RS10230 at 1968.162 kb on + strand, within ABIE40_RS10230 at 1968.163 kb on - strand, within ABIE40_RS10230 at 1968.272 kb on + strand, within ABIE40_RS10230 at 1968.273 kb on - strand, within ABIE40_RS10230 at 1968.273 kb on - strand, within ABIE40_RS10230 at 1968.479 kb on + strand, within ABIE40_RS10230 at 1968.479 kb on + strand, within ABIE40_RS10230 at 1968.479 kb on + strand, within ABIE40_RS10230 at 1968.479 kb on + strand, within ABIE40_RS10230 at 1968.479 kb on + strand, within ABIE40_RS10230 at 1968.480 kb on - strand, within ABIE40_RS10230 at 1968.480 kb on - strand, within ABIE40_RS10230 at 1968.480 kb on - strand, within ABIE40_RS10230 at 1968.480 kb on - strand, within ABIE40_RS10230 at 1968.480 kb on - strand, within ABIE40_RS10230 at 1968.480 kb on - strand, within ABIE40_RS10230 at 1968.518 kb on + strand, within ABIE40_RS10230 at 1968.518 kb on + strand, within ABIE40_RS10230 at 1968.522 kb on + strand, within ABIE40_RS10230 at 1968.522 kb on + strand, within ABIE40_RS10230 at 1968.523 kb on - strand, within ABIE40_RS10230 at 1968.530 kb on + strand, within ABIE40_RS10230 at 1968.566 kb on + strand, within ABIE40_RS10230 at 1968.579 kb on + strand, within ABIE40_RS10230 at 1968.579 kb on + strand, within ABIE40_RS10230 at 1968.579 kb on + strand, within ABIE40_RS10230 at 1968.683 kb on + strand, within ABIE40_RS10230 at 1968.684 kb on - strand, within ABIE40_RS10230 at 1968.891 kb on + strand at 1968.891 kb on + strand at 1968.892 kb on - strand at 1968.892 kb on - strand at 1969.094 kb on + strand, within ABIE40_RS10235 at 1969.094 kb on + strand, within ABIE40_RS10235 at 1969.136 kb on - strand, within ABIE40_RS10235 at 1969.159 kb on + strand, within ABIE40_RS10235 at 1969.159 kb on + strand, within ABIE40_RS10235 at 1969.315 kb on + strand, within ABIE40_RS10235 at 1969.315 kb on + strand, within ABIE40_RS10235 at 1969.316 kb on - strand, within ABIE40_RS10235 at 1969.713 kb on + strand, within ABIE40_RS10235 at 1969.736 kb on - strand, within ABIE40_RS10235 at 1969.736 kb on - strand, within ABIE40_RS10235 at 1969.736 kb on - strand, within ABIE40_RS10235 at 1969.807 kb on + strand at 1969.807 kb on + strand at 1969.808 kb on - strand at 1969.852 kb on + strand at 1969.852 kb on + strand at 1969.852 kb on + strand at 1969.852 kb on + strand at 1969.852 kb on + strand at 1969.852 kb on + strand at 1969.852 kb on + strand at 1969.853 kb on - strand at 1969.853 kb on - strand at 1969.853 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with 3-octanone 5.3129 mM remove 1,967,962 + -2.4 1,967,962 + -2.7 1,967,963 - +0.3 1,968,014 + +0.1 1,968,014 + -2.0 1,968,014 + -2.0 1,968,015 - +0.1 1,968,015 - -0.7 1,968,121 - ABIE40_RS10230 0.12 -1.3 1,968,121 - ABIE40_RS10230 0.12 -0.0 1,968,121 - ABIE40_RS10230 0.12 +0.5 1,968,121 - ABIE40_RS10230 0.12 +0.4 1,968,162 + ABIE40_RS10230 0.17 +0.5 1,968,162 + ABIE40_RS10230 0.17 -0.4 1,968,163 - ABIE40_RS10230 0.17 -0.1 1,968,272 + ABIE40_RS10230 0.29 -0.0 1,968,273 - ABIE40_RS10230 0.29 +0.0 1,968,273 - ABIE40_RS10230 0.29 -0.1 1,968,479 + ABIE40_RS10230 0.53 +1.4 1,968,479 + ABIE40_RS10230 0.53 +0.8 1,968,479 + ABIE40_RS10230 0.53 -2.2 1,968,479 + ABIE40_RS10230 0.53 +0.6 1,968,479 + ABIE40_RS10230 0.53 +0.5 1,968,480 - ABIE40_RS10230 0.53 +0.4 1,968,480 - ABIE40_RS10230 0.53 +0.8 1,968,480 - ABIE40_RS10230 0.53 -0.8 1,968,480 - ABIE40_RS10230 0.53 +2.1 1,968,480 - ABIE40_RS10230 0.53 +1.1 1,968,480 - ABIE40_RS10230 0.53 +0.5 1,968,518 + ABIE40_RS10230 0.57 -2.3 1,968,518 + ABIE40_RS10230 0.57 +0.1 1,968,522 + ABIE40_RS10230 0.58 -0.9 1,968,522 + ABIE40_RS10230 0.58 +0.4 1,968,523 - ABIE40_RS10230 0.58 +1.1 1,968,530 + ABIE40_RS10230 0.58 -2.9 1,968,566 + ABIE40_RS10230 0.62 +0.1 1,968,579 + ABIE40_RS10230 0.64 +0.3 1,968,579 + ABIE40_RS10230 0.64 -0.0 1,968,579 + ABIE40_RS10230 0.64 -1.0 1,968,683 + ABIE40_RS10230 0.76 -0.2 1,968,684 - ABIE40_RS10230 0.76 -2.4 1,968,891 + -0.5 1,968,891 + +0.8 1,968,892 - +2.1 1,968,892 - -1.5 1,969,094 + ABIE40_RS10235 0.15 +0.4 1,969,094 + ABIE40_RS10235 0.15 -1.5 1,969,136 - ABIE40_RS10235 0.19 +0.8 1,969,159 + ABIE40_RS10235 0.22 -3.5 1,969,159 + ABIE40_RS10235 0.22 -0.5 1,969,315 + ABIE40_RS10235 0.38 +0.8 1,969,315 + ABIE40_RS10235 0.38 -1.1 1,969,316 - ABIE40_RS10235 0.39 -1.2 1,969,713 + ABIE40_RS10235 0.81 -0.3 1,969,736 - ABIE40_RS10235 0.84 -0.5 1,969,736 - ABIE40_RS10235 0.84 -2.6 1,969,736 - ABIE40_RS10235 0.84 -0.7 1,969,807 + -0.7 1,969,807 + +0.4 1,969,808 - +0.5 1,969,852 + +0.1 1,969,852 + +0.3 1,969,852 + +1.1 1,969,852 + -2.2 1,969,852 + +1.0 1,969,852 + +0.9 1,969,852 + +0.7 1,969,853 - +0.7 1,969,853 - -0.9 1,969,853 - -0.2
Or see this region's nucleotide sequence