Experiment: R2A with 3-octanone 5.3129 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE40_RS09595 and ABIE40_RS09600 are separated by 6 nucleotides ABIE40_RS09600 and ABIE40_RS09605 are separated by 136 nucleotides ABIE40_RS09605 and ABIE40_RS09610 are separated by 84 nucleotides
ABIE40_RS09595: ABIE40_RS09595 - adenylate/guanylate cyclase domain-containing protein, at 1,851,631 to 1,853,493
_RS09595
ABIE40_RS09600: ABIE40_RS09600 - FecR family protein, at 1,853,500 to 1,854,021
_RS09600
ABIE40_RS09605: ABIE40_RS09605 - citrate synthase, at 1,854,158 to 1,855,294
_RS09605
ABIE40_RS09610: ABIE40_RS09610 - citrate synthase/methylcitrate synthase, at 1,855,379 to 1,856,467
_RS09610
Position (kb)
1854
1855
1856 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1853.277 kb on - strand, within ABIE40_RS09595 at 1853.476 kb on - strand at 1853.502 kb on - strand at 1853.755 kb on - strand, within ABIE40_RS09600 at 1853.875 kb on - strand, within ABIE40_RS09600 at 1854.064 kb on - strand at 1854.064 kb on - strand at 1854.064 kb on - strand at 1854.107 kb on + strand at 1854.107 kb on + strand at 1854.108 kb on - strand at 1854.108 kb on - strand at 1854.108 kb on - strand at 1854.108 kb on - strand at 1854.108 kb on - strand at 1854.108 kb on - strand at 1854.128 kb on + strand at 1854.189 kb on + strand at 1854.190 kb on - strand at 1854.586 kb on + strand, within ABIE40_RS09605 at 1854.587 kb on - strand, within ABIE40_RS09605 at 1854.587 kb on - strand, within ABIE40_RS09605 at 1854.814 kb on + strand, within ABIE40_RS09605 at 1854.815 kb on - strand, within ABIE40_RS09605 at 1854.815 kb on - strand, within ABIE40_RS09605 at 1854.815 kb on - strand, within ABIE40_RS09605 at 1854.815 kb on - strand, within ABIE40_RS09605 at 1854.830 kb on + strand, within ABIE40_RS09605 at 1854.830 kb on + strand, within ABIE40_RS09605 at 1854.830 kb on + strand, within ABIE40_RS09605 at 1854.831 kb on - strand, within ABIE40_RS09605 at 1854.831 kb on - strand, within ABIE40_RS09605 at 1855.026 kb on + strand, within ABIE40_RS09605 at 1855.026 kb on + strand, within ABIE40_RS09605 at 1855.027 kb on - strand, within ABIE40_RS09605 at 1855.027 kb on - strand, within ABIE40_RS09605 at 1855.029 kb on + strand, within ABIE40_RS09605 at 1855.029 kb on + strand, within ABIE40_RS09605 at 1855.030 kb on - strand, within ABIE40_RS09605 at 1855.167 kb on + strand, within ABIE40_RS09605 at 1855.167 kb on + strand, within ABIE40_RS09605 at 1855.168 kb on - strand, within ABIE40_RS09605 at 1855.168 kb on - strand, within ABIE40_RS09605 at 1855.210 kb on + strand at 1855.233 kb on + strand at 1855.233 kb on + strand at 1855.233 kb on + strand at 1855.233 kb on + strand at 1855.234 kb on - strand at 1855.355 kb on + strand at 1855.467 kb on + strand at 1855.499 kb on + strand, within ABIE40_RS09610 at 1855.499 kb on + strand, within ABIE40_RS09610 at 1855.499 kb on + strand, within ABIE40_RS09610 at 1855.499 kb on + strand, within ABIE40_RS09610 at 1855.499 kb on + strand, within ABIE40_RS09610 at 1855.499 kb on + strand, within ABIE40_RS09610 at 1855.499 kb on + strand, within ABIE40_RS09610 at 1855.500 kb on - strand, within ABIE40_RS09610 at 1855.500 kb on - strand, within ABIE40_RS09610 at 1855.500 kb on - strand, within ABIE40_RS09610 at 1855.871 kb on + strand, within ABIE40_RS09610 at 1855.871 kb on + strand, within ABIE40_RS09610 at 1855.871 kb on + strand, within ABIE40_RS09610 at 1855.872 kb on - strand, within ABIE40_RS09610 at 1855.872 kb on - strand, within ABIE40_RS09610 at 1855.872 kb on - strand, within ABIE40_RS09610 at 1855.872 kb on - strand, within ABIE40_RS09610 at 1856.026 kb on + strand, within ABIE40_RS09610 at 1856.027 kb on - strand, within ABIE40_RS09610 at 1856.123 kb on + strand, within ABIE40_RS09610 at 1856.123 kb on + strand, within ABIE40_RS09610 at 1856.124 kb on - strand, within ABIE40_RS09610 at 1856.124 kb on - strand, within ABIE40_RS09610 at 1856.207 kb on - strand, within ABIE40_RS09610
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with 3-octanone 5.3129 mM remove 1,853,277 - ABIE40_RS09595 0.88 +1.2 1,853,476 - -0.4 1,853,502 - +0.5 1,853,755 - ABIE40_RS09600 0.49 -0.5 1,853,875 - ABIE40_RS09600 0.72 -1.7 1,854,064 - -0.4 1,854,064 - +0.1 1,854,064 - +0.6 1,854,107 + -0.8 1,854,107 + -1.0 1,854,108 - +0.7 1,854,108 - -0.9 1,854,108 - -1.7 1,854,108 - -0.5 1,854,108 - +0.9 1,854,108 - +0.3 1,854,128 + -1.0 1,854,189 + -0.6 1,854,190 - +0.1 1,854,586 + ABIE40_RS09605 0.38 -2.3 1,854,587 - ABIE40_RS09605 0.38 +0.1 1,854,587 - ABIE40_RS09605 0.38 -0.1 1,854,814 + ABIE40_RS09605 0.58 +1.3 1,854,815 - ABIE40_RS09605 0.58 +0.2 1,854,815 - ABIE40_RS09605 0.58 +0.5 1,854,815 - ABIE40_RS09605 0.58 +1.4 1,854,815 - ABIE40_RS09605 0.58 +0.5 1,854,830 + ABIE40_RS09605 0.59 -0.8 1,854,830 + ABIE40_RS09605 0.59 +1.2 1,854,830 + ABIE40_RS09605 0.59 -1.6 1,854,831 - ABIE40_RS09605 0.59 -2.3 1,854,831 - ABIE40_RS09605 0.59 +0.5 1,855,026 + ABIE40_RS09605 0.76 -1.2 1,855,026 + ABIE40_RS09605 0.76 +0.7 1,855,027 - ABIE40_RS09605 0.76 +0.7 1,855,027 - ABIE40_RS09605 0.76 +0.2 1,855,029 + ABIE40_RS09605 0.77 -0.5 1,855,029 + ABIE40_RS09605 0.77 -0.9 1,855,030 - ABIE40_RS09605 0.77 -0.5 1,855,167 + ABIE40_RS09605 0.89 -1.4 1,855,167 + ABIE40_RS09605 0.89 -1.3 1,855,168 - ABIE40_RS09605 0.89 -1.1 1,855,168 - ABIE40_RS09605 0.89 +0.1 1,855,210 + -0.4 1,855,233 + -0.8 1,855,233 + +1.3 1,855,233 + -0.7 1,855,233 + +0.8 1,855,234 - +1.8 1,855,355 + +1.1 1,855,467 + +0.1 1,855,499 + ABIE40_RS09610 0.11 -0.7 1,855,499 + ABIE40_RS09610 0.11 -1.5 1,855,499 + ABIE40_RS09610 0.11 -1.2 1,855,499 + ABIE40_RS09610 0.11 -0.5 1,855,499 + ABIE40_RS09610 0.11 -2.5 1,855,499 + ABIE40_RS09610 0.11 -1.9 1,855,499 + ABIE40_RS09610 0.11 -1.2 1,855,500 - ABIE40_RS09610 0.11 +0.1 1,855,500 - ABIE40_RS09610 0.11 +1.9 1,855,500 - ABIE40_RS09610 0.11 +3.2 1,855,871 + ABIE40_RS09610 0.45 -0.2 1,855,871 + ABIE40_RS09610 0.45 -1.1 1,855,871 + ABIE40_RS09610 0.45 +1.6 1,855,872 - ABIE40_RS09610 0.45 +0.1 1,855,872 - ABIE40_RS09610 0.45 -0.2 1,855,872 - ABIE40_RS09610 0.45 +0.9 1,855,872 - ABIE40_RS09610 0.45 +0.3 1,856,026 + ABIE40_RS09610 0.59 -0.3 1,856,027 - ABIE40_RS09610 0.60 -0.5 1,856,123 + ABIE40_RS09610 0.68 +0.1 1,856,123 + ABIE40_RS09610 0.68 +1.1 1,856,124 - ABIE40_RS09610 0.68 +0.4 1,856,124 - ABIE40_RS09610 0.68 +1.2 1,856,207 - ABIE40_RS09610 0.76 +0.2
Or see this region's nucleotide sequence