Experiment: R2A with 3-octanone 5.3129 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE40_RS06765 and ABIE40_RS06770 are separated by 190 nucleotides ABIE40_RS06770 and ABIE40_RS06775 are separated by 10 nucleotides ABIE40_RS06775 and ABIE40_RS06780 are separated by 103 nucleotides
ABIE40_RS06765: ABIE40_RS06765 - outer membrane protein assembly factor BamE, at 1,326,517 to 1,327,041
_RS06765
ABIE40_RS06770: ABIE40_RS06770 - ubiquinol-cytochrome C chaperone family protein, at 1,327,232 to 1,327,821
_RS06770
ABIE40_RS06775: ABIE40_RS06775 - DUF177 domain-containing protein, at 1,327,832 to 1,328,386
_RS06775
ABIE40_RS06780: ABIE40_RS06780 - phosphate acyltransferase PlsX, at 1,328,490 to 1,329,536
_RS06780
Position (kb)
1327
1328
1329 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1327.384 kb on + strand, within ABIE40_RS06770 at 1327.384 kb on + strand, within ABIE40_RS06770 at 1327.538 kb on - strand, within ABIE40_RS06770 at 1327.609 kb on + strand, within ABIE40_RS06770 at 1327.610 kb on - strand, within ABIE40_RS06770 at 1327.627 kb on + strand, within ABIE40_RS06770 at 1327.784 kb on + strand at 1327.785 kb on - strand at 1327.865 kb on + strand at 1327.865 kb on + strand at 1327.865 kb on + strand at 1327.866 kb on - strand at 1327.885 kb on + strand at 1327.885 kb on + strand at 1327.886 kb on - strand at 1327.914 kb on + strand, within ABIE40_RS06775 at 1327.914 kb on + strand, within ABIE40_RS06775 at 1327.937 kb on - strand, within ABIE40_RS06775 at 1328.021 kb on - strand, within ABIE40_RS06775 at 1328.315 kb on - strand, within ABIE40_RS06775 at 1328.315 kb on - strand, within ABIE40_RS06775 at 1328.388 kb on + strand at 1328.399 kb on + strand at 1328.419 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with 3-octanone 5.3129 mM remove 1,327,384 + ABIE40_RS06770 0.26 +1.2 1,327,384 + ABIE40_RS06770 0.26 +0.4 1,327,538 - ABIE40_RS06770 0.52 +0.3 1,327,609 + ABIE40_RS06770 0.64 -0.9 1,327,610 - ABIE40_RS06770 0.64 -0.3 1,327,627 + ABIE40_RS06770 0.67 -0.3 1,327,784 + -0.5 1,327,785 - -2.3 1,327,865 + +0.6 1,327,865 + +0.6 1,327,865 + -1.8 1,327,866 - -1.0 1,327,885 + -0.1 1,327,885 + -2.5 1,327,886 - -0.8 1,327,914 + ABIE40_RS06775 0.15 -1.6 1,327,914 + ABIE40_RS06775 0.15 +2.9 1,327,937 - ABIE40_RS06775 0.19 -1.5 1,328,021 - ABIE40_RS06775 0.34 -1.3 1,328,315 - ABIE40_RS06775 0.87 -1.0 1,328,315 - ABIE40_RS06775 0.87 -0.3 1,328,388 + -1.7 1,328,399 + -0.1 1,328,419 + -0.8
Or see this region's nucleotide sequence