Experiment: R2A with 3-octanone 5.3129 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE40_RS06050 and ABIE40_RS06055 are separated by 165 nucleotides ABIE40_RS06055 and ABIE40_RS06060 are separated by 160 nucleotides ABIE40_RS06060 and ABIE40_RS06065 are separated by 25 nucleotides ABIE40_RS06065 and ABIE40_RS06070 are separated by 115 nucleotides
ABIE40_RS06050: ABIE40_RS06050 - bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase, at 1,190,386 to 1,192,620
_RS06050
ABIE40_RS06055: ABIE40_RS06055 - DUF3563 family protein, at 1,192,786 to 1,192,935
_RS06055
ABIE40_RS06060: ABIE40_RS06060 - DUF2062 domain-containing protein, at 1,193,096 to 1,193,686
_RS06060
ABIE40_RS06065: ABIE40_RS06065 - holo-ACP synthase, at 1,193,712 to 1,194,116
_RS06065
ABIE40_RS06070: ABIE40_RS06070 - signal peptidase I, at 1,194,232 to 1,194,975
_RS06070
Position (kb)
1193
1194 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1192.636 kb on + strand at 1192.637 kb on - strand at 1192.706 kb on + strand at 1192.710 kb on - strand at 1192.721 kb on - strand at 1192.721 kb on - strand at 1192.741 kb on - strand at 1192.785 kb on + strand at 1192.797 kb on + strand at 1192.871 kb on - strand, within ABIE40_RS06055 at 1192.891 kb on + strand, within ABIE40_RS06055 at 1192.892 kb on - strand, within ABIE40_RS06055 at 1192.979 kb on + strand at 1192.979 kb on + strand at 1192.980 kb on - strand at 1192.996 kb on + strand at 1192.997 kb on - strand at 1193.084 kb on + strand at 1193.085 kb on - strand at 1193.085 kb on - strand at 1193.088 kb on + strand at 1193.088 kb on + strand at 1193.315 kb on + strand, within ABIE40_RS06060 at 1193.315 kb on + strand, within ABIE40_RS06060 at 1193.315 kb on + strand, within ABIE40_RS06060 at 1193.316 kb on - strand, within ABIE40_RS06060 at 1193.316 kb on - strand, within ABIE40_RS06060 at 1193.381 kb on + strand, within ABIE40_RS06060 at 1193.382 kb on - strand, within ABIE40_RS06060 at 1193.382 kb on - strand, within ABIE40_RS06060 at 1193.441 kb on - strand, within ABIE40_RS06060 at 1193.588 kb on + strand, within ABIE40_RS06060 at 1193.588 kb on + strand, within ABIE40_RS06060 at 1193.588 kb on + strand, within ABIE40_RS06060 at 1193.589 kb on - strand, within ABIE40_RS06060 at 1194.138 kb on + strand at 1194.138 kb on + strand at 1194.139 kb on - strand at 1194.139 kb on - strand at 1194.189 kb on + strand at 1194.189 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with 3-octanone 5.3129 mM remove 1,192,636 + -1.1 1,192,637 - +0.1 1,192,706 + -3.1 1,192,710 - -1.9 1,192,721 - -0.1 1,192,721 - -0.4 1,192,741 - -0.7 1,192,785 + +1.2 1,192,797 + -1.7 1,192,871 - ABIE40_RS06055 0.57 +1.9 1,192,891 + ABIE40_RS06055 0.70 -1.2 1,192,892 - ABIE40_RS06055 0.71 +0.9 1,192,979 + +0.9 1,192,979 + -1.3 1,192,980 - +0.3 1,192,996 + -1.4 1,192,997 - -0.4 1,193,084 + +0.1 1,193,085 - -0.7 1,193,085 - -1.2 1,193,088 + +0.5 1,193,088 + +1.0 1,193,315 + ABIE40_RS06060 0.37 +1.1 1,193,315 + ABIE40_RS06060 0.37 +1.5 1,193,315 + ABIE40_RS06060 0.37 +0.9 1,193,316 - ABIE40_RS06060 0.37 -0.5 1,193,316 - ABIE40_RS06060 0.37 -2.3 1,193,381 + ABIE40_RS06060 0.48 +1.9 1,193,382 - ABIE40_RS06060 0.48 -0.9 1,193,382 - ABIE40_RS06060 0.48 -0.8 1,193,441 - ABIE40_RS06060 0.58 +0.3 1,193,588 + ABIE40_RS06060 0.83 -0.5 1,193,588 + ABIE40_RS06060 0.83 -0.0 1,193,588 + ABIE40_RS06060 0.83 -1.2 1,193,589 - ABIE40_RS06060 0.83 -0.0 1,194,138 + -0.8 1,194,138 + -1.3 1,194,139 - +0.3 1,194,139 - +0.9 1,194,189 + -1.3 1,194,189 + -1.4
Or see this region's nucleotide sequence