Experiment: R2A with 3-octanone 5.3129 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE40_RS04295 and ABIE40_RS04300 are separated by 4 nucleotides ABIE40_RS04300 and ABIE40_RS04305 overlap by 4 nucleotides ABIE40_RS04305 and ABIE40_RS04310 are separated by 212 nucleotides
ABIE40_RS04295: ABIE40_RS04295 - DUF721 domain-containing protein, at 847,189 to 847,674
_RS04295
ABIE40_RS04300: ABIE40_RS04300 - type II toxin-antitoxin system VapC family toxin, at 847,679 to 848,098
_RS04300
ABIE40_RS04305: ABIE40_RS04305 - Arc family DNA-binding protein, at 848,095 to 848,334
_RS04305
ABIE40_RS04310: ABIE40_RS04310 - DsbA family protein, at 848,547 to 849,239
_RS04310
Position (kb) 848
849 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 847.097 kb on + strand at 847.119 kb on + strand at 847.119 kb on + strand at 847.119 kb on + strand at 847.119 kb on + strand at 847.119 kb on + strand at 847.119 kb on + strand at 847.119 kb on + strand at 847.120 kb on - strand at 847.120 kb on - strand at 847.120 kb on - strand at 847.121 kb on + strand at 847.121 kb on + strand at 847.121 kb on + strand at 847.121 kb on + strand at 847.122 kb on - strand at 847.122 kb on - strand at 847.122 kb on - strand at 847.195 kb on + strand at 847.680 kb on + strand at 847.680 kb on + strand at 847.680 kb on + strand at 847.680 kb on + strand at 847.680 kb on + strand at 847.680 kb on + strand at 847.681 kb on - strand at 847.681 kb on - strand at 847.998 kb on + strand, within ABIE40_RS04300 at 847.999 kb on - strand, within ABIE40_RS04300 at 847.999 kb on - strand, within ABIE40_RS04300 at 847.999 kb on - strand, within ABIE40_RS04300 at 847.999 kb on - strand, within ABIE40_RS04300 at 848.118 kb on + strand at 848.118 kb on + strand at 848.118 kb on + strand at 848.118 kb on + strand at 848.142 kb on + strand, within ABIE40_RS04305 at 848.142 kb on + strand, within ABIE40_RS04305 at 848.232 kb on + strand, within ABIE40_RS04305 at 848.235 kb on + strand, within ABIE40_RS04305 at 848.323 kb on + strand at 848.323 kb on + strand at 848.324 kb on - strand at 848.325 kb on + strand at 848.326 kb on - strand at 848.346 kb on + strand at 848.346 kb on + strand at 848.346 kb on + strand at 848.346 kb on + strand at 848.347 kb on - strand at 848.347 kb on - strand at 848.347 kb on - strand at 848.347 kb on - strand at 848.355 kb on + strand at 848.411 kb on + strand at 848.432 kb on + strand at 848.432 kb on + strand at 848.432 kb on + strand at 848.433 kb on - strand at 848.433 kb on - strand at 848.433 kb on - strand at 848.433 kb on - strand at 848.434 kb on + strand at 848.434 kb on + strand at 848.434 kb on + strand at 848.504 kb on + strand at 848.506 kb on + strand at 848.506 kb on + strand at 849.292 kb on + strand at 849.292 kb on + strand at 849.292 kb on + strand at 849.293 kb on - strand at 849.293 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with 3-octanone 5.3129 mM remove 847,097 + -0.5 847,119 + +0.4 847,119 + -0.4 847,119 + +1.8 847,119 + -2.7 847,119 + -1.1 847,119 + +1.4 847,119 + -0.8 847,120 - -2.2 847,120 - -0.9 847,120 - -1.8 847,121 + +0.0 847,121 + +0.2 847,121 + -1.3 847,121 + -0.8 847,122 - +0.8 847,122 - +1.2 847,122 - -0.8 847,195 + -0.2 847,680 + -1.3 847,680 + -1.4 847,680 + -1.4 847,680 + +1.0 847,680 + -0.3 847,680 + +1.4 847,681 - +0.6 847,681 - -1.3 847,998 + ABIE40_RS04300 0.76 -3.3 847,999 - ABIE40_RS04300 0.76 -0.4 847,999 - ABIE40_RS04300 0.76 -0.6 847,999 - ABIE40_RS04300 0.76 +2.0 847,999 - ABIE40_RS04300 0.76 -2.3 848,118 + -1.6 848,118 + +1.5 848,118 + +0.4 848,118 + -1.7 848,142 + ABIE40_RS04305 0.20 -1.8 848,142 + ABIE40_RS04305 0.20 -1.8 848,232 + ABIE40_RS04305 0.57 -0.5 848,235 + ABIE40_RS04305 0.58 -1.7 848,323 + +1.8 848,323 + -1.9 848,324 - -1.2 848,325 + -0.1 848,326 - +1.0 848,346 + -2.4 848,346 + -0.3 848,346 + -1.3 848,346 + +1.9 848,347 - -0.6 848,347 - -0.9 848,347 - -0.9 848,347 - -0.8 848,355 + -1.6 848,411 + -1.4 848,432 + -1.8 848,432 + -1.8 848,432 + -0.2 848,433 - -0.1 848,433 - +0.8 848,433 - -0.8 848,433 - +1.8 848,434 + -3.1 848,434 + +0.2 848,434 + -0.1 848,504 + -0.5 848,506 + -3.5 848,506 + -0.4 849,292 + -1.9 849,292 + -1.3 849,292 + +0.2 849,293 - -2.3 849,293 - -1.0
Or see this region's nucleotide sequence