Experiment: R2A with 1-octen-3-ol 0.904549 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE40_RS09460 and ABIE40_RS09465 overlap by 4 nucleotides ABIE40_RS09465 and ABIE40_RS09470 are separated by 221 nucleotides
ABIE40_RS09460: ABIE40_RS09460 - pantoate--beta-alanine ligase, at 1,825,934 to 1,826,809
_RS09460
ABIE40_RS09465: ABIE40_RS09465 - 3-methyl-2-oxobutanoate hydroxymethyltransferase, at 1,826,806 to 1,827,627
_RS09465
ABIE40_RS09470: ABIE40_RS09470 - ISNCY family transposase, at 1,827,849 to 1,829,222
_RS09470
Position (kb)
1826
1827
1828 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1825.838 kb on - strand at 1825.847 kb on + strand at 1825.847 kb on + strand at 1825.903 kb on + strand at 1825.932 kb on + strand at 1825.933 kb on - strand at 1825.933 kb on - strand at 1826.028 kb on - strand, within ABIE40_RS09460 at 1826.028 kb on - strand, within ABIE40_RS09460 at 1826.144 kb on + strand, within ABIE40_RS09460 at 1826.144 kb on + strand, within ABIE40_RS09460 at 1826.228 kb on + strand, within ABIE40_RS09460 at 1826.228 kb on + strand, within ABIE40_RS09460 at 1826.228 kb on + strand, within ABIE40_RS09460 at 1826.228 kb on + strand, within ABIE40_RS09460 at 1826.228 kb on + strand, within ABIE40_RS09460 at 1826.229 kb on - strand, within ABIE40_RS09460 at 1826.229 kb on - strand, within ABIE40_RS09460 at 1826.229 kb on - strand, within ABIE40_RS09460 at 1826.229 kb on - strand, within ABIE40_RS09460 at 1826.344 kb on + strand, within ABIE40_RS09460 at 1826.345 kb on - strand, within ABIE40_RS09460 at 1826.385 kb on - strand, within ABIE40_RS09460 at 1826.385 kb on - strand, within ABIE40_RS09460 at 1826.385 kb on - strand, within ABIE40_RS09460 at 1826.460 kb on + strand, within ABIE40_RS09460 at 1826.460 kb on + strand, within ABIE40_RS09460 at 1826.461 kb on - strand, within ABIE40_RS09460 at 1826.461 kb on - strand, within ABIE40_RS09460 at 1826.501 kb on + strand, within ABIE40_RS09460 at 1826.501 kb on + strand, within ABIE40_RS09460 at 1826.502 kb on - strand, within ABIE40_RS09460 at 1826.502 kb on - strand, within ABIE40_RS09460 at 1826.502 kb on - strand, within ABIE40_RS09460 at 1826.759 kb on - strand at 1826.759 kb on - strand at 1826.793 kb on + strand at 1826.793 kb on + strand at 1826.794 kb on - strand at 1826.893 kb on + strand, within ABIE40_RS09465 at 1826.893 kb on + strand, within ABIE40_RS09465 at 1826.893 kb on + strand, within ABIE40_RS09465 at 1826.893 kb on + strand, within ABIE40_RS09465 at 1826.894 kb on - strand, within ABIE40_RS09465 at 1826.894 kb on - strand, within ABIE40_RS09465 at 1826.894 kb on - strand, within ABIE40_RS09465 at 1826.895 kb on + strand, within ABIE40_RS09465 at 1826.895 kb on + strand, within ABIE40_RS09465 at 1826.896 kb on - strand, within ABIE40_RS09465 at 1826.896 kb on - strand, within ABIE40_RS09465 at 1826.972 kb on - strand, within ABIE40_RS09465 at 1827.085 kb on + strand, within ABIE40_RS09465 at 1827.085 kb on + strand, within ABIE40_RS09465 at 1827.086 kb on - strand, within ABIE40_RS09465 at 1827.232 kb on - strand, within ABIE40_RS09465 at 1827.411 kb on + strand, within ABIE40_RS09465 at 1827.563 kb on - strand at 1827.563 kb on - strand at 1827.657 kb on + strand at 1827.657 kb on + strand at 1827.657 kb on + strand at 1827.678 kb on + strand at 1827.679 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with 1-octen-3-ol 0.904549 mM remove 1,825,838 - -1.3 1,825,847 + -2.6 1,825,847 + +2.0 1,825,903 + -0.5 1,825,932 + +0.7 1,825,933 - -2.2 1,825,933 - -1.7 1,826,028 - ABIE40_RS09460 0.11 +0.3 1,826,028 - ABIE40_RS09460 0.11 -1.9 1,826,144 + ABIE40_RS09460 0.24 -0.6 1,826,144 + ABIE40_RS09460 0.24 +1.6 1,826,228 + ABIE40_RS09460 0.34 -2.0 1,826,228 + ABIE40_RS09460 0.34 -0.0 1,826,228 + ABIE40_RS09460 0.34 -0.9 1,826,228 + ABIE40_RS09460 0.34 -1.4 1,826,228 + ABIE40_RS09460 0.34 +1.2 1,826,229 - ABIE40_RS09460 0.34 -2.4 1,826,229 - ABIE40_RS09460 0.34 -1.6 1,826,229 - ABIE40_RS09460 0.34 +0.1 1,826,229 - ABIE40_RS09460 0.34 +0.0 1,826,344 + ABIE40_RS09460 0.47 -1.3 1,826,345 - ABIE40_RS09460 0.47 -2.4 1,826,385 - ABIE40_RS09460 0.51 -1.0 1,826,385 - ABIE40_RS09460 0.51 -0.6 1,826,385 - ABIE40_RS09460 0.51 -1.1 1,826,460 + ABIE40_RS09460 0.60 -0.4 1,826,460 + ABIE40_RS09460 0.60 -1.1 1,826,461 - ABIE40_RS09460 0.60 +0.0 1,826,461 - ABIE40_RS09460 0.60 -1.4 1,826,501 + ABIE40_RS09460 0.65 +0.4 1,826,501 + ABIE40_RS09460 0.65 -0.5 1,826,502 - ABIE40_RS09460 0.65 +0.4 1,826,502 - ABIE40_RS09460 0.65 +1.7 1,826,502 - ABIE40_RS09460 0.65 -0.6 1,826,759 - +1.6 1,826,759 - +1.0 1,826,793 + +0.0 1,826,793 + +1.1 1,826,794 - +1.0 1,826,893 + ABIE40_RS09465 0.11 -0.6 1,826,893 + ABIE40_RS09465 0.11 +0.9 1,826,893 + ABIE40_RS09465 0.11 +0.5 1,826,893 + ABIE40_RS09465 0.11 +0.9 1,826,894 - ABIE40_RS09465 0.11 -0.8 1,826,894 - ABIE40_RS09465 0.11 +2.5 1,826,894 - ABIE40_RS09465 0.11 -0.6 1,826,895 + ABIE40_RS09465 0.11 +1.0 1,826,895 + ABIE40_RS09465 0.11 -0.0 1,826,896 - ABIE40_RS09465 0.11 -2.1 1,826,896 - ABIE40_RS09465 0.11 +0.5 1,826,972 - ABIE40_RS09465 0.20 -2.1 1,827,085 + ABIE40_RS09465 0.34 +0.3 1,827,085 + ABIE40_RS09465 0.34 -0.0 1,827,086 - ABIE40_RS09465 0.34 +0.6 1,827,232 - ABIE40_RS09465 0.52 +0.0 1,827,411 + ABIE40_RS09465 0.74 -0.4 1,827,563 - +0.8 1,827,563 - -0.5 1,827,657 + -0.2 1,827,657 + -1.3 1,827,657 + -0.8 1,827,678 + -1.3 1,827,679 - -0.9
Or see this region's nucleotide sequence