Experiment: R2A with 1-octen-3-ol 0.904549 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE40_RS04575 and ABIE40_RS04580 are separated by 149 nucleotides ABIE40_RS04580 and ABIE40_RS04585 overlap by 26 nucleotides ABIE40_RS04585 and ABIE40_RS04590 are separated by 120 nucleotides ABIE40_RS04590 and ABIE40_RS04595 are separated by 148 nucleotides
ABIE40_RS04575: ABIE40_RS04575 - asparaginase, at 900,339 to 901,343
_RS04575
ABIE40_RS04580: ABIE40_RS04580 - hypothetical protein, at 901,493 to 902,242
_RS04580
ABIE40_RS04585: ABIE40_RS04585 - DeoR/GlpR family DNA-binding transcription regulator, at 902,217 to 902,972
_RS04585
ABIE40_RS04590: ABIE40_RS04590 - DUF4406 domain-containing protein, at 903,093 to 903,458
_RS04590
ABIE40_RS04595: ABIE40_RS04595 - glutathione S-transferase N-terminal domain-containing protein, at 903,607 to 904,227
_RS04595
Position (kb)
902
903 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 901.366 kb on + strand at 901.366 kb on + strand at 901.367 kb on - strand at 901.367 kb on - strand at 901.367 kb on - strand at 901.367 kb on - strand at 901.393 kb on - strand at 901.402 kb on + strand at 901.402 kb on + strand at 901.402 kb on + strand at 901.402 kb on + strand at 901.402 kb on + strand at 901.403 kb on - strand at 901.403 kb on - strand at 901.403 kb on - strand at 901.403 kb on - strand at 901.447 kb on + strand at 901.447 kb on + strand at 901.447 kb on + strand at 901.447 kb on + strand at 901.447 kb on + strand at 901.448 kb on - strand at 901.474 kb on + strand at 901.474 kb on + strand at 901.474 kb on + strand at 901.475 kb on - strand at 901.475 kb on - strand at 901.475 kb on - strand at 901.492 kb on + strand at 901.492 kb on + strand at 901.492 kb on + strand at 901.493 kb on - strand at 901.493 kb on - strand at 901.493 kb on - strand at 901.493 kb on - strand at 901.493 kb on - strand at 901.509 kb on - strand at 901.509 kb on - strand at 901.509 kb on - strand at 901.568 kb on + strand, within ABIE40_RS04580 at 901.569 kb on - strand, within ABIE40_RS04580 at 901.569 kb on - strand, within ABIE40_RS04580 at 901.569 kb on - strand, within ABIE40_RS04580 at 901.569 kb on - strand, within ABIE40_RS04580 at 901.721 kb on + strand, within ABIE40_RS04580 at 901.722 kb on - strand, within ABIE40_RS04580 at 901.769 kb on + strand, within ABIE40_RS04580 at 901.769 kb on + strand, within ABIE40_RS04580 at 901.769 kb on + strand, within ABIE40_RS04580 at 901.770 kb on - strand, within ABIE40_RS04580 at 901.770 kb on - strand, within ABIE40_RS04580 at 901.770 kb on - strand, within ABIE40_RS04580 at 901.770 kb on - strand, within ABIE40_RS04580 at 901.770 kb on - strand, within ABIE40_RS04580 at 901.886 kb on + strand, within ABIE40_RS04580 at 901.886 kb on + strand, within ABIE40_RS04580 at 901.887 kb on - strand, within ABIE40_RS04580 at 901.974 kb on - strand, within ABIE40_RS04580 at 902.126 kb on + strand, within ABIE40_RS04580 at 902.126 kb on + strand, within ABIE40_RS04580 at 902.126 kb on + strand, within ABIE40_RS04580 at 902.126 kb on + strand, within ABIE40_RS04580 at 902.127 kb on - strand, within ABIE40_RS04580 at 902.127 kb on - strand, within ABIE40_RS04580 at 902.128 kb on + strand, within ABIE40_RS04580 at 902.128 kb on + strand, within ABIE40_RS04580 at 902.129 kb on - strand, within ABIE40_RS04580 at 902.129 kb on - strand, within ABIE40_RS04580 at 902.129 kb on - strand, within ABIE40_RS04580 at 902.368 kb on + strand, within ABIE40_RS04585 at 902.368 kb on + strand, within ABIE40_RS04585 at 902.368 kb on + strand, within ABIE40_RS04585 at 902.368 kb on + strand, within ABIE40_RS04585 at 902.368 kb on + strand, within ABIE40_RS04585 at 902.368 kb on + strand, within ABIE40_RS04585 at 902.369 kb on - strand, within ABIE40_RS04585 at 902.369 kb on - strand, within ABIE40_RS04585 at 902.369 kb on - strand, within ABIE40_RS04585 at 902.369 kb on - strand, within ABIE40_RS04585 at 902.369 kb on - strand, within ABIE40_RS04585 at 902.369 kb on - strand, within ABIE40_RS04585 at 902.369 kb on - strand, within ABIE40_RS04585 at 902.369 kb on - strand, within ABIE40_RS04585 at 902.369 kb on - strand, within ABIE40_RS04585 at 902.394 kb on - strand, within ABIE40_RS04585 at 902.543 kb on - strand, within ABIE40_RS04585 at 902.543 kb on - strand, within ABIE40_RS04585 at 902.543 kb on - strand, within ABIE40_RS04585 at 902.676 kb on - strand, within ABIE40_RS04585 at 902.870 kb on + strand, within ABIE40_RS04585 at 902.871 kb on - strand, within ABIE40_RS04585 at 903.186 kb on + strand, within ABIE40_RS04590 at 903.186 kb on + strand, within ABIE40_RS04590 at 903.186 kb on + strand, within ABIE40_RS04590 at 903.186 kb on + strand, within ABIE40_RS04590 at 903.186 kb on + strand, within ABIE40_RS04590 at 903.187 kb on - strand, within ABIE40_RS04590 at 903.300 kb on + strand, within ABIE40_RS04590 at 903.300 kb on + strand, within ABIE40_RS04590 at 903.301 kb on - strand, within ABIE40_RS04590 at 903.457 kb on - strand at 903.490 kb on + strand at 903.490 kb on + strand at 903.491 kb on - strand at 903.496 kb on + strand at 903.496 kb on + strand at 903.512 kb on + strand at 903.512 kb on + strand at 903.512 kb on + strand at 903.512 kb on + strand at 903.513 kb on - strand at 903.513 kb on - strand at 903.513 kb on - strand at 903.577 kb on + strand at 903.578 kb on - strand at 903.578 kb on - strand at 903.579 kb on + strand at 903.579 kb on + strand at 903.579 kb on + strand at 903.579 kb on + strand at 903.579 kb on + strand at 903.579 kb on + strand at 903.579 kb on + strand at 903.579 kb on + strand at 903.579 kb on + strand at 903.579 kb on + strand at 903.579 kb on + strand at 903.579 kb on + strand at 903.579 kb on + strand at 903.579 kb on + strand at 903.579 kb on + strand at 903.579 kb on + strand at 903.579 kb on + strand at 903.579 kb on + strand at 903.579 kb on + strand at 903.579 kb on + strand at 903.579 kb on + strand at 903.579 kb on + strand at 903.580 kb on - strand at 903.580 kb on - strand at 903.580 kb on - strand at 903.580 kb on - strand at 903.580 kb on - strand at 903.580 kb on - strand at 903.580 kb on - strand at 903.580 kb on - strand at 903.580 kb on - strand at 903.580 kb on - strand at 903.580 kb on - strand at 903.580 kb on - strand at 903.580 kb on - strand at 903.580 kb on - strand at 903.580 kb on - strand at 903.580 kb on - strand at 903.580 kb on - strand at 903.619 kb on + strand at 903.620 kb on - strand at 903.620 kb on - strand at 903.620 kb on - strand at 903.682 kb on + strand, within ABIE40_RS04595 at 903.733 kb on + strand, within ABIE40_RS04595 at 903.817 kb on + strand, within ABIE40_RS04595 at 903.817 kb on + strand, within ABIE40_RS04595 at 903.817 kb on + strand, within ABIE40_RS04595 at 903.818 kb on - strand, within ABIE40_RS04595 at 903.818 kb on - strand, within ABIE40_RS04595 at 903.818 kb on - strand, within ABIE40_RS04595 at 903.819 kb on + strand, within ABIE40_RS04595 at 903.819 kb on + strand, within ABIE40_RS04595 at 903.819 kb on + strand, within ABIE40_RS04595 at 903.820 kb on - strand, within ABIE40_RS04595 at 903.901 kb on + strand, within ABIE40_RS04595 at 903.901 kb on + strand, within ABIE40_RS04595 at 903.901 kb on + strand, within ABIE40_RS04595 at 903.902 kb on - strand, within ABIE40_RS04595 at 903.936 kb on + strand, within ABIE40_RS04595 at 903.936 kb on + strand, within ABIE40_RS04595
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with 1-octen-3-ol 0.904549 mM remove 901,366 + +0.4 901,366 + +0.6 901,367 - -0.2 901,367 - +0.1 901,367 - -0.1 901,367 - -1.0 901,393 - +0.5 901,402 + +0.0 901,402 + -1.3 901,402 + +0.6 901,402 + +0.1 901,402 + +0.1 901,403 - -2.0 901,403 - +0.4 901,403 - -0.4 901,403 - -0.2 901,447 + +1.8 901,447 + -3.0 901,447 + -0.3 901,447 + -0.7 901,447 + +0.0 901,448 - +0.5 901,474 + -1.1 901,474 + -1.5 901,474 + -0.4 901,475 - +0.2 901,475 - +1.1 901,475 - +0.6 901,492 + -0.6 901,492 + +0.4 901,492 + +0.8 901,493 - +0.0 901,493 - +0.2 901,493 - +0.4 901,493 - +0.3 901,493 - -0.3 901,509 - +1.5 901,509 - +0.9 901,509 - +1.1 901,568 + ABIE40_RS04580 0.10 -1.5 901,569 - ABIE40_RS04580 0.10 -2.0 901,569 - ABIE40_RS04580 0.10 -0.8 901,569 - ABIE40_RS04580 0.10 +0.1 901,569 - ABIE40_RS04580 0.10 +0.5 901,721 + ABIE40_RS04580 0.30 -0.3 901,722 - ABIE40_RS04580 0.31 -0.6 901,769 + ABIE40_RS04580 0.37 -0.4 901,769 + ABIE40_RS04580 0.37 -0.6 901,769 + ABIE40_RS04580 0.37 -0.5 901,770 - ABIE40_RS04580 0.37 -0.2 901,770 - ABIE40_RS04580 0.37 +0.4 901,770 - ABIE40_RS04580 0.37 +0.7 901,770 - ABIE40_RS04580 0.37 -0.7 901,770 - ABIE40_RS04580 0.37 -1.9 901,886 + ABIE40_RS04580 0.52 -0.2 901,886 + ABIE40_RS04580 0.52 -0.2 901,887 - ABIE40_RS04580 0.53 +0.3 901,974 - ABIE40_RS04580 0.64 +1.7 902,126 + ABIE40_RS04580 0.84 -1.6 902,126 + ABIE40_RS04580 0.84 -0.3 902,126 + ABIE40_RS04580 0.84 +0.1 902,126 + ABIE40_RS04580 0.84 +0.4 902,127 - ABIE40_RS04580 0.85 -0.2 902,127 - ABIE40_RS04580 0.85 -2.9 902,128 + ABIE40_RS04580 0.85 -0.2 902,128 + ABIE40_RS04580 0.85 -0.9 902,129 - ABIE40_RS04580 0.85 +0.5 902,129 - ABIE40_RS04580 0.85 +0.4 902,129 - ABIE40_RS04580 0.85 +0.8 902,368 + ABIE40_RS04585 0.20 -0.5 902,368 + ABIE40_RS04585 0.20 +0.2 902,368 + ABIE40_RS04585 0.20 +1.2 902,368 + ABIE40_RS04585 0.20 -0.3 902,368 + ABIE40_RS04585 0.20 +1.8 902,368 + ABIE40_RS04585 0.20 -2.0 902,369 - ABIE40_RS04585 0.20 -0.3 902,369 - ABIE40_RS04585 0.20 +0.9 902,369 - ABIE40_RS04585 0.20 -0.7 902,369 - ABIE40_RS04585 0.20 -1.3 902,369 - ABIE40_RS04585 0.20 -0.3 902,369 - ABIE40_RS04585 0.20 -0.4 902,369 - ABIE40_RS04585 0.20 -0.4 902,369 - ABIE40_RS04585 0.20 -0.8 902,369 - ABIE40_RS04585 0.20 -0.7 902,394 - ABIE40_RS04585 0.23 -0.7 902,543 - ABIE40_RS04585 0.43 +0.7 902,543 - ABIE40_RS04585 0.43 +0.2 902,543 - ABIE40_RS04585 0.43 -1.3 902,676 - ABIE40_RS04585 0.61 -0.2 902,870 + ABIE40_RS04585 0.86 -2.3 902,871 - ABIE40_RS04585 0.87 -0.6 903,186 + ABIE40_RS04590 0.25 -0.6 903,186 + ABIE40_RS04590 0.25 +0.5 903,186 + ABIE40_RS04590 0.25 -0.2 903,186 + ABIE40_RS04590 0.25 -0.0 903,186 + ABIE40_RS04590 0.25 -0.2 903,187 - ABIE40_RS04590 0.26 +0.6 903,300 + ABIE40_RS04590 0.57 +0.8 903,300 + ABIE40_RS04590 0.57 +0.7 903,301 - ABIE40_RS04590 0.57 -0.2 903,457 - +0.4 903,490 + -2.3 903,490 + -2.8 903,491 - -0.4 903,496 + +2.4 903,496 + -0.9 903,512 + +0.1 903,512 + +0.2 903,512 + -1.4 903,512 + -0.5 903,513 - +1.8 903,513 - +0.8 903,513 - +0.8 903,577 + +1.8 903,578 - +0.5 903,578 - +0.0 903,579 + -0.3 903,579 + +1.1 903,579 + +1.4 903,579 + -1.0 903,579 + -0.6 903,579 + -0.8 903,579 + -2.3 903,579 + -0.2 903,579 + -1.4 903,579 + -0.6 903,579 + -0.8 903,579 + +0.5 903,579 + -1.4 903,579 + -0.8 903,579 + +0.8 903,579 + -0.8 903,579 + -0.4 903,579 + -0.4 903,579 + -2.9 903,579 + +0.2 903,579 + +1.2 903,579 + +1.4 903,580 - -0.1 903,580 - +0.3 903,580 - +0.4 903,580 - +0.4 903,580 - +0.1 903,580 - -1.8 903,580 - +0.5 903,580 - -0.5 903,580 - -2.8 903,580 - -0.1 903,580 - +0.2 903,580 - +0.5 903,580 - +0.3 903,580 - +0.5 903,580 - +0.2 903,580 - +0.6 903,580 - +0.8 903,619 + -0.0 903,620 - +0.7 903,620 - +2.4 903,620 - +3.1 903,682 + ABIE40_RS04595 0.12 -1.8 903,733 + ABIE40_RS04595 0.20 +0.4 903,817 + ABIE40_RS04595 0.34 +1.2 903,817 + ABIE40_RS04595 0.34 +0.8 903,817 + ABIE40_RS04595 0.34 -2.3 903,818 - ABIE40_RS04595 0.34 -1.4 903,818 - ABIE40_RS04595 0.34 -0.8 903,818 - ABIE40_RS04595 0.34 +0.5 903,819 + ABIE40_RS04595 0.34 -0.3 903,819 + ABIE40_RS04595 0.34 -0.8 903,819 + ABIE40_RS04595 0.34 +1.8 903,820 - ABIE40_RS04595 0.34 -1.2 903,901 + ABIE40_RS04595 0.47 -2.3 903,901 + ABIE40_RS04595 0.47 -0.5 903,901 + ABIE40_RS04595 0.47 -2.6 903,902 - ABIE40_RS04595 0.48 +0.1 903,936 + ABIE40_RS04595 0.53 -0.7 903,936 + ABIE40_RS04595 0.53 +0.4
Or see this region's nucleotide sequence