Experiment: R2A with 1-octen-3-ol 0.904549 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE40_RS10975 and ABIE40_RS10980 are separated by 238 nucleotides ABIE40_RS10980 and ABIE40_RS10985 are separated by 85 nucleotides
ABIE40_RS10975: ABIE40_RS10975 - glycoside hydrolase family 43 protein, at 2,126,363 to 2,127,976
_RS10975
ABIE40_RS10980: ABIE40_RS10980 - L-glyceraldehyde 3-phosphate reductase, at 2,128,215 to 2,129,246
_RS10980
ABIE40_RS10985: ABIE40_RS10985 - Gfo/Idh/MocA family oxidoreductase, at 2,129,332 to 2,130,465
_RS10985
Position (kb)
2128
2129
2130 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 2127.289 kb on - strand, within ABIE40_RS10975 at 2127.302 kb on - strand, within ABIE40_RS10975 at 2127.336 kb on + strand, within ABIE40_RS10975 at 2127.336 kb on + strand, within ABIE40_RS10975 at 2127.337 kb on - strand, within ABIE40_RS10975 at 2127.337 kb on - strand, within ABIE40_RS10975 at 2127.337 kb on - strand, within ABIE40_RS10975 at 2127.366 kb on + strand, within ABIE40_RS10975 at 2127.367 kb on - strand, within ABIE40_RS10975 at 2127.367 kb on - strand, within ABIE40_RS10975 at 2127.369 kb on + strand, within ABIE40_RS10975 at 2127.370 kb on - strand, within ABIE40_RS10975 at 2127.370 kb on - strand, within ABIE40_RS10975 at 2127.370 kb on - strand, within ABIE40_RS10975 at 2127.370 kb on - strand, within ABIE40_RS10975 at 2127.372 kb on + strand, within ABIE40_RS10975 at 2127.372 kb on + strand, within ABIE40_RS10975 at 2127.373 kb on - strand, within ABIE40_RS10975 at 2127.373 kb on - strand, within ABIE40_RS10975 at 2127.373 kb on - strand, within ABIE40_RS10975 at 2127.373 kb on - strand, within ABIE40_RS10975 at 2127.433 kb on + strand, within ABIE40_RS10975 at 2127.433 kb on + strand, within ABIE40_RS10975 at 2127.434 kb on - strand, within ABIE40_RS10975 at 2127.526 kb on - strand, within ABIE40_RS10975 at 2127.621 kb on + strand, within ABIE40_RS10975 at 2127.621 kb on + strand, within ABIE40_RS10975 at 2127.621 kb on + strand, within ABIE40_RS10975 at 2127.621 kb on + strand, within ABIE40_RS10975 at 2127.622 kb on - strand, within ABIE40_RS10975 at 2127.622 kb on - strand, within ABIE40_RS10975 at 2127.622 kb on - strand, within ABIE40_RS10975 at 2127.622 kb on - strand, within ABIE40_RS10975 at 2127.622 kb on - strand, within ABIE40_RS10975 at 2127.622 kb on - strand, within ABIE40_RS10975 at 2127.622 kb on - strand, within ABIE40_RS10975 at 2127.626 kb on - strand, within ABIE40_RS10975 at 2127.669 kb on + strand, within ABIE40_RS10975 at 2127.669 kb on + strand, within ABIE40_RS10975 at 2127.669 kb on + strand, within ABIE40_RS10975 at 2127.669 kb on + strand, within ABIE40_RS10975 at 2127.669 kb on + strand, within ABIE40_RS10975 at 2127.669 kb on + strand, within ABIE40_RS10975 at 2127.670 kb on - strand, within ABIE40_RS10975 at 2127.670 kb on - strand, within ABIE40_RS10975 at 2127.670 kb on - strand, within ABIE40_RS10975 at 2127.670 kb on - strand, within ABIE40_RS10975 at 2127.673 kb on + strand, within ABIE40_RS10975 at 2127.674 kb on - strand, within ABIE40_RS10975 at 2127.674 kb on - strand, within ABIE40_RS10975 at 2127.715 kb on + strand, within ABIE40_RS10975 at 2127.715 kb on + strand, within ABIE40_RS10975 at 2127.717 kb on + strand, within ABIE40_RS10975 at 2127.718 kb on - strand, within ABIE40_RS10975 at 2127.718 kb on - strand, within ABIE40_RS10975 at 2127.718 kb on - strand, within ABIE40_RS10975 at 2127.718 kb on - strand, within ABIE40_RS10975 at 2127.906 kb on + strand at 2127.906 kb on + strand at 2127.906 kb on + strand at 2127.906 kb on + strand at 2127.906 kb on + strand at 2127.906 kb on + strand at 2127.906 kb on + strand at 2127.906 kb on + strand at 2127.906 kb on + strand at 2127.907 kb on - strand at 2127.907 kb on - strand at 2127.907 kb on - strand at 2127.907 kb on - strand at 2127.907 kb on - strand at 2127.907 kb on - strand at 2127.907 kb on - strand at 2127.907 kb on - strand at 2127.909 kb on + strand at 2127.909 kb on + strand at 2127.909 kb on + strand at 2127.909 kb on + strand at 2127.909 kb on + strand at 2127.910 kb on - strand at 2127.910 kb on - strand at 2127.910 kb on - strand at 2127.910 kb on - strand at 2128.005 kb on + strand at 2128.005 kb on - strand at 2128.206 kb on - strand at 2128.267 kb on + strand at 2128.267 kb on + strand at 2128.267 kb on + strand at 2128.268 kb on - strand at 2128.299 kb on - strand at 2128.310 kb on + strand at 2128.311 kb on - strand at 2128.661 kb on - strand, within ABIE40_RS10980 at 2128.739 kb on - strand, within ABIE40_RS10980 at 2128.756 kb on + strand, within ABIE40_RS10980 at 2128.756 kb on + strand, within ABIE40_RS10980 at 2128.756 kb on + strand, within ABIE40_RS10980 at 2128.757 kb on - strand, within ABIE40_RS10980 at 2128.757 kb on - strand, within ABIE40_RS10980 at 2128.840 kb on + strand, within ABIE40_RS10980 at 2128.841 kb on - strand, within ABIE40_RS10980 at 2128.841 kb on - strand, within ABIE40_RS10980 at 2128.848 kb on - strand, within ABIE40_RS10980 at 2128.848 kb on - strand, within ABIE40_RS10980 at 2128.849 kb on - strand, within ABIE40_RS10980 at 2128.856 kb on - strand, within ABIE40_RS10980 at 2128.903 kb on + strand, within ABIE40_RS10980 at 2128.904 kb on - strand, within ABIE40_RS10980 at 2128.904 kb on - strand, within ABIE40_RS10980 at 2128.904 kb on - strand, within ABIE40_RS10980 at 2128.904 kb on - strand, within ABIE40_RS10980 at 2128.927 kb on + strand, within ABIE40_RS10980 at 2128.928 kb on - strand, within ABIE40_RS10980 at 2128.928 kb on - strand, within ABIE40_RS10980 at 2128.948 kb on + strand, within ABIE40_RS10980 at 2129.050 kb on + strand, within ABIE40_RS10980 at 2129.050 kb on + strand, within ABIE40_RS10980 at 2129.050 kb on + strand, within ABIE40_RS10980 at 2129.050 kb on + strand, within ABIE40_RS10980 at 2129.050 kb on + strand, within ABIE40_RS10980 at 2129.050 kb on + strand, within ABIE40_RS10980 at 2129.050 kb on + strand, within ABIE40_RS10980 at 2129.051 kb on - strand, within ABIE40_RS10980 at 2129.051 kb on - strand, within ABIE40_RS10980 at 2129.105 kb on + strand, within ABIE40_RS10980 at 2129.105 kb on + strand, within ABIE40_RS10980 at 2129.105 kb on + strand, within ABIE40_RS10980 at 2129.106 kb on - strand, within ABIE40_RS10980 at 2129.106 kb on - strand, within ABIE40_RS10980 at 2129.106 kb on - strand, within ABIE40_RS10980 at 2129.203 kb on + strand at 2129.203 kb on + strand at 2129.253 kb on - strand at 2129.253 kb on - strand at 2129.253 kb on - strand at 2129.253 kb on - strand at 2129.260 kb on + strand at 2129.298 kb on + strand at 2129.298 kb on + strand at 2129.299 kb on - strand at 2129.299 kb on - strand at 2129.299 kb on - strand at 2129.299 kb on - strand at 2129.299 kb on - strand at 2129.537 kb on + strand, within ABIE40_RS10985 at 2129.537 kb on + strand, within ABIE40_RS10985 at 2129.537 kb on + strand, within ABIE40_RS10985 at 2129.538 kb on - strand, within ABIE40_RS10985 at 2129.613 kb on + strand, within ABIE40_RS10985 at 2129.614 kb on - strand, within ABIE40_RS10985 at 2129.614 kb on - strand, within ABIE40_RS10985 at 2129.614 kb on - strand, within ABIE40_RS10985 at 2129.672 kb on + strand, within ABIE40_RS10985 at 2129.672 kb on + strand, within ABIE40_RS10985 at 2129.672 kb on + strand, within ABIE40_RS10985 at 2129.672 kb on + strand, within ABIE40_RS10985 at 2129.672 kb on + strand, within ABIE40_RS10985 at 2129.673 kb on - strand, within ABIE40_RS10985 at 2129.673 kb on - strand, within ABIE40_RS10985 at 2129.673 kb on - strand, within ABIE40_RS10985 at 2129.673 kb on - strand, within ABIE40_RS10985 at 2129.673 kb on - strand, within ABIE40_RS10985 at 2129.673 kb on - strand, within ABIE40_RS10985 at 2129.673 kb on - strand, within ABIE40_RS10985 at 2129.703 kb on + strand, within ABIE40_RS10985 at 2129.704 kb on - strand, within ABIE40_RS10985 at 2129.704 kb on - strand, within ABIE40_RS10985 at 2129.704 kb on - strand, within ABIE40_RS10985 at 2129.704 kb on - strand, within ABIE40_RS10985 at 2129.921 kb on + strand, within ABIE40_RS10985 at 2129.921 kb on + strand, within ABIE40_RS10985 at 2129.921 kb on + strand, within ABIE40_RS10985 at 2129.921 kb on + strand, within ABIE40_RS10985 at 2129.922 kb on - strand, within ABIE40_RS10985 at 2129.922 kb on - strand, within ABIE40_RS10985 at 2129.922 kb on - strand, within ABIE40_RS10985 at 2129.922 kb on - strand, within ABIE40_RS10985 at 2129.922 kb on - strand, within ABIE40_RS10985 at 2129.922 kb on - strand, within ABIE40_RS10985 at 2129.922 kb on - strand, within ABIE40_RS10985 at 2129.922 kb on - strand, within ABIE40_RS10985 at 2129.924 kb on + strand, within ABIE40_RS10985 at 2129.924 kb on + strand, within ABIE40_RS10985 at 2129.924 kb on + strand, within ABIE40_RS10985 at 2129.925 kb on - strand, within ABIE40_RS10985 at 2129.925 kb on - strand, within ABIE40_RS10985 at 2130.014 kb on + strand, within ABIE40_RS10985 at 2130.015 kb on - strand, within ABIE40_RS10985 at 2130.015 kb on - strand, within ABIE40_RS10985 at 2130.015 kb on - strand, within ABIE40_RS10985 at 2130.015 kb on - strand, within ABIE40_RS10985 at 2130.032 kb on - strand, within ABIE40_RS10985 at 2130.032 kb on - strand, within ABIE40_RS10985 at 2130.191 kb on + strand, within ABIE40_RS10985 at 2130.192 kb on - strand, within ABIE40_RS10985
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with 1-octen-3-ol 0.904549 mM remove 2,127,289 - ABIE40_RS10975 0.57 +4.3 2,127,302 - ABIE40_RS10975 0.58 +2.3 2,127,336 + ABIE40_RS10975 0.60 +0.0 2,127,336 + ABIE40_RS10975 0.60 +0.1 2,127,337 - ABIE40_RS10975 0.60 -2.0 2,127,337 - ABIE40_RS10975 0.60 -0.6 2,127,337 - ABIE40_RS10975 0.60 +0.3 2,127,366 + ABIE40_RS10975 0.62 -1.6 2,127,367 - ABIE40_RS10975 0.62 +0.2 2,127,367 - ABIE40_RS10975 0.62 -0.1 2,127,369 + ABIE40_RS10975 0.62 -1.0 2,127,370 - ABIE40_RS10975 0.62 -1.5 2,127,370 - ABIE40_RS10975 0.62 -1.8 2,127,370 - ABIE40_RS10975 0.62 -0.3 2,127,370 - ABIE40_RS10975 0.62 +3.0 2,127,372 + ABIE40_RS10975 0.63 +0.2 2,127,372 + ABIE40_RS10975 0.63 -0.3 2,127,373 - ABIE40_RS10975 0.63 -0.4 2,127,373 - ABIE40_RS10975 0.63 -0.7 2,127,373 - ABIE40_RS10975 0.63 -0.5 2,127,373 - ABIE40_RS10975 0.63 -1.2 2,127,433 + ABIE40_RS10975 0.66 -0.4 2,127,433 + ABIE40_RS10975 0.66 -0.4 2,127,434 - ABIE40_RS10975 0.66 -0.6 2,127,526 - ABIE40_RS10975 0.72 +2.0 2,127,621 + ABIE40_RS10975 0.78 -0.4 2,127,621 + ABIE40_RS10975 0.78 +0.7 2,127,621 + ABIE40_RS10975 0.78 -1.5 2,127,621 + ABIE40_RS10975 0.78 -3.1 2,127,622 - ABIE40_RS10975 0.78 -2.4 2,127,622 - ABIE40_RS10975 0.78 -0.4 2,127,622 - ABIE40_RS10975 0.78 +0.6 2,127,622 - ABIE40_RS10975 0.78 -0.4 2,127,622 - ABIE40_RS10975 0.78 -0.4 2,127,622 - ABIE40_RS10975 0.78 -1.4 2,127,622 - ABIE40_RS10975 0.78 -0.9 2,127,626 - ABIE40_RS10975 0.78 -1.2 2,127,669 + ABIE40_RS10975 0.81 -1.7 2,127,669 + ABIE40_RS10975 0.81 -1.5 2,127,669 + ABIE40_RS10975 0.81 -0.4 2,127,669 + ABIE40_RS10975 0.81 -0.2 2,127,669 + ABIE40_RS10975 0.81 -0.4 2,127,669 + ABIE40_RS10975 0.81 -2.1 2,127,670 - ABIE40_RS10975 0.81 +1.4 2,127,670 - ABIE40_RS10975 0.81 -1.0 2,127,670 - ABIE40_RS10975 0.81 +0.4 2,127,670 - ABIE40_RS10975 0.81 -3.0 2,127,673 + ABIE40_RS10975 0.81 -2.2 2,127,674 - ABIE40_RS10975 0.81 -0.8 2,127,674 - ABIE40_RS10975 0.81 +0.4 2,127,715 + ABIE40_RS10975 0.84 -0.7 2,127,715 + ABIE40_RS10975 0.84 -1.4 2,127,717 + ABIE40_RS10975 0.84 +0.7 2,127,718 - ABIE40_RS10975 0.84 -0.0 2,127,718 - ABIE40_RS10975 0.84 -1.7 2,127,718 - ABIE40_RS10975 0.84 -0.6 2,127,718 - ABIE40_RS10975 0.84 +0.9 2,127,906 + -0.3 2,127,906 + +0.0 2,127,906 + -0.2 2,127,906 + -0.6 2,127,906 + -0.4 2,127,906 + +0.8 2,127,906 + -3.2 2,127,906 + +0.2 2,127,906 + +1.8 2,127,907 - +0.4 2,127,907 - +0.1 2,127,907 - -2.9 2,127,907 - +3.5 2,127,907 - +1.0 2,127,907 - -0.3 2,127,907 - +0.4 2,127,907 - +1.0 2,127,909 + -0.1 2,127,909 + -0.9 2,127,909 + -0.4 2,127,909 + +0.6 2,127,909 + -0.2 2,127,910 - -0.4 2,127,910 - -1.2 2,127,910 - +0.4 2,127,910 - -2.4 2,128,005 + -0.7 2,128,005 - -1.4 2,128,206 - +1.5 2,128,267 + -0.0 2,128,267 + -1.1 2,128,267 + +2.4 2,128,268 - -0.2 2,128,299 - -0.7 2,128,310 + -0.6 2,128,311 - -0.5 2,128,661 - ABIE40_RS10980 0.43 -0.3 2,128,739 - ABIE40_RS10980 0.51 +0.5 2,128,756 + ABIE40_RS10980 0.52 +1.4 2,128,756 + ABIE40_RS10980 0.52 -0.3 2,128,756 + ABIE40_RS10980 0.52 -0.7 2,128,757 - ABIE40_RS10980 0.53 +0.3 2,128,757 - ABIE40_RS10980 0.53 -0.8 2,128,840 + ABIE40_RS10980 0.61 -0.6 2,128,841 - ABIE40_RS10980 0.61 -0.4 2,128,841 - ABIE40_RS10980 0.61 -0.0 2,128,848 - ABIE40_RS10980 0.61 -1.4 2,128,848 - ABIE40_RS10980 0.61 +0.9 2,128,849 - ABIE40_RS10980 0.61 -1.0 2,128,856 - ABIE40_RS10980 0.62 -0.5 2,128,903 + ABIE40_RS10980 0.67 -1.0 2,128,904 - ABIE40_RS10980 0.67 -3.2 2,128,904 - ABIE40_RS10980 0.67 +0.3 2,128,904 - ABIE40_RS10980 0.67 +0.4 2,128,904 - ABIE40_RS10980 0.67 -1.3 2,128,927 + ABIE40_RS10980 0.69 +1.1 2,128,928 - ABIE40_RS10980 0.69 +1.2 2,128,928 - ABIE40_RS10980 0.69 -2.7 2,128,948 + ABIE40_RS10980 0.71 -0.2 2,129,050 + ABIE40_RS10980 0.81 +0.6 2,129,050 + ABIE40_RS10980 0.81 +1.4 2,129,050 + ABIE40_RS10980 0.81 +1.0 2,129,050 + ABIE40_RS10980 0.81 +0.7 2,129,050 + ABIE40_RS10980 0.81 +0.6 2,129,050 + ABIE40_RS10980 0.81 +2.0 2,129,050 + ABIE40_RS10980 0.81 +0.7 2,129,051 - ABIE40_RS10980 0.81 -2.9 2,129,051 - ABIE40_RS10980 0.81 -3.6 2,129,105 + ABIE40_RS10980 0.86 -0.7 2,129,105 + ABIE40_RS10980 0.86 +0.3 2,129,105 + ABIE40_RS10980 0.86 +0.8 2,129,106 - ABIE40_RS10980 0.86 +1.6 2,129,106 - ABIE40_RS10980 0.86 -2.6 2,129,106 - ABIE40_RS10980 0.86 +0.7 2,129,203 + -0.0 2,129,203 + +0.6 2,129,253 - -2.0 2,129,253 - +0.1 2,129,253 - +0.4 2,129,253 - +0.2 2,129,260 + -0.1 2,129,298 + +0.6 2,129,298 + -2.7 2,129,299 - -2.7 2,129,299 - +2.4 2,129,299 - +1.0 2,129,299 - +0.6 2,129,299 - -0.2 2,129,537 + ABIE40_RS10985 0.18 +0.5 2,129,537 + ABIE40_RS10985 0.18 +1.0 2,129,537 + ABIE40_RS10985 0.18 -0.4 2,129,538 - ABIE40_RS10985 0.18 +0.8 2,129,613 + ABIE40_RS10985 0.25 -0.8 2,129,614 - ABIE40_RS10985 0.25 +0.1 2,129,614 - ABIE40_RS10985 0.25 +1.0 2,129,614 - ABIE40_RS10985 0.25 -1.0 2,129,672 + ABIE40_RS10985 0.30 -0.7 2,129,672 + ABIE40_RS10985 0.30 +0.3 2,129,672 + ABIE40_RS10985 0.30 -0.5 2,129,672 + ABIE40_RS10985 0.30 +0.9 2,129,672 + ABIE40_RS10985 0.30 +0.3 2,129,673 - ABIE40_RS10985 0.30 +0.2 2,129,673 - ABIE40_RS10985 0.30 -0.2 2,129,673 - ABIE40_RS10985 0.30 +1.0 2,129,673 - ABIE40_RS10985 0.30 -2.8 2,129,673 - ABIE40_RS10985 0.30 -2.5 2,129,673 - ABIE40_RS10985 0.30 +0.7 2,129,673 - ABIE40_RS10985 0.30 +0.8 2,129,703 + ABIE40_RS10985 0.33 +0.2 2,129,704 - ABIE40_RS10985 0.33 +0.2 2,129,704 - ABIE40_RS10985 0.33 +0.7 2,129,704 - ABIE40_RS10985 0.33 -0.0 2,129,704 - ABIE40_RS10985 0.33 +0.5 2,129,921 + ABIE40_RS10985 0.52 -1.0 2,129,921 + ABIE40_RS10985 0.52 +2.2 2,129,921 + ABIE40_RS10985 0.52 -1.7 2,129,921 + ABIE40_RS10985 0.52 -1.4 2,129,922 - ABIE40_RS10985 0.52 -0.2 2,129,922 - ABIE40_RS10985 0.52 -1.0 2,129,922 - ABIE40_RS10985 0.52 +1.0 2,129,922 - ABIE40_RS10985 0.52 +1.1 2,129,922 - ABIE40_RS10985 0.52 -1.6 2,129,922 - ABIE40_RS10985 0.52 +1.1 2,129,922 - ABIE40_RS10985 0.52 +0.6 2,129,922 - ABIE40_RS10985 0.52 -0.4 2,129,924 + ABIE40_RS10985 0.52 +0.3 2,129,924 + ABIE40_RS10985 0.52 +0.7 2,129,924 + ABIE40_RS10985 0.52 +1.9 2,129,925 - ABIE40_RS10985 0.52 +0.4 2,129,925 - ABIE40_RS10985 0.52 +0.5 2,130,014 + ABIE40_RS10985 0.60 +1.4 2,130,015 - ABIE40_RS10985 0.60 +0.5 2,130,015 - ABIE40_RS10985 0.60 -1.9 2,130,015 - ABIE40_RS10985 0.60 -1.9 2,130,015 - ABIE40_RS10985 0.60 -0.2 2,130,032 - ABIE40_RS10985 0.62 -0.7 2,130,032 - ABIE40_RS10985 0.62 -0.3 2,130,191 + ABIE40_RS10985 0.76 -3.2 2,130,192 - ABIE40_RS10985 0.76 +0.9
Or see this region's nucleotide sequence