Experiment: R2A with 1-octen-3-ol 0.904549 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE40_RS10345 and ABIE40_RS10350 are separated by 275 nucleotides ABIE40_RS10350 and ABIE40_RS10355 are separated by 66 nucleotides ABIE40_RS10355 and ABIE40_RS10360 overlap by 4 nucleotides
ABIE40_RS10345: ABIE40_RS10345 - RNA polymerase-binding protein DksA, at 1,993,912 to 1,994,331
_RS10345
ABIE40_RS10350: ABIE40_RS10350 - histidine phosphatase family protein, at 1,994,607 to 1,995,101
_RS10350
ABIE40_RS10355: ABIE40_RS10355 - YcjX family protein, at 1,995,168 to 1,996,643
_RS10355
ABIE40_RS10360: ABIE40_RS10360 - TIGR01620 family protein, at 1,996,640 to 1,997,722
_RS10360
Position (kb)
1995
1996
1997 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1994.195 kb on - strand, within ABIE40_RS10345 at 1994.195 kb on - strand, within ABIE40_RS10345 at 1994.195 kb on - strand, within ABIE40_RS10345 at 1994.195 kb on - strand, within ABIE40_RS10345 at 1994.215 kb on - strand, within ABIE40_RS10345 at 1994.215 kb on - strand, within ABIE40_RS10345 at 1994.256 kb on - strand, within ABIE40_RS10345 at 1994.303 kb on + strand at 1994.304 kb on - strand at 1994.369 kb on + strand at 1994.369 kb on + strand at 1994.370 kb on - strand at 1994.370 kb on - strand at 1994.370 kb on - strand at 1994.370 kb on - strand at 1994.370 kb on - strand at 1994.370 kb on - strand at 1994.412 kb on + strand at 1994.606 kb on + strand at 1994.607 kb on - strand at 1994.640 kb on + strand at 1994.641 kb on - strand at 1994.718 kb on + strand, within ABIE40_RS10350 at 1994.860 kb on - strand, within ABIE40_RS10350 at 1994.874 kb on + strand, within ABIE40_RS10350 at 1994.874 kb on + strand, within ABIE40_RS10350 at 1994.874 kb on + strand, within ABIE40_RS10350 at 1994.875 kb on - strand, within ABIE40_RS10350 at 1994.895 kb on + strand, within ABIE40_RS10350 at 1994.896 kb on - strand, within ABIE40_RS10350 at 1994.896 kb on - strand, within ABIE40_RS10350 at 1994.896 kb on - strand, within ABIE40_RS10350 at 1994.896 kb on - strand, within ABIE40_RS10350 at 1994.948 kb on + strand, within ABIE40_RS10350 at 1995.005 kb on + strand, within ABIE40_RS10350 at 1995.134 kb on + strand at 1995.134 kb on + strand at 1995.134 kb on + strand at 1995.134 kb on + strand at 1995.134 kb on + strand at 1995.134 kb on + strand at 1995.134 kb on + strand at 1995.134 kb on + strand at 1995.135 kb on - strand at 1995.135 kb on - strand at 1995.135 kb on - strand at 1995.135 kb on - strand at 1995.135 kb on - strand at 1995.135 kb on - strand at 1995.135 kb on - strand at 1995.135 kb on - strand at 1995.135 kb on - strand at 1995.135 kb on - strand at 1995.135 kb on - strand at 1995.135 kb on - strand at 1995.136 kb on + strand at 1995.137 kb on - strand at 1995.137 kb on - strand at 1995.137 kb on - strand at 1995.137 kb on - strand at 1995.519 kb on + strand, within ABIE40_RS10355 at 1995.519 kb on + strand, within ABIE40_RS10355 at 1995.585 kb on + strand, within ABIE40_RS10355 at 1995.585 kb on + strand, within ABIE40_RS10355 at 1995.636 kb on - strand, within ABIE40_RS10355 at 1995.650 kb on + strand, within ABIE40_RS10355 at 1995.650 kb on + strand, within ABIE40_RS10355 at 1995.650 kb on + strand, within ABIE40_RS10355 at 1995.650 kb on + strand, within ABIE40_RS10355 at 1995.651 kb on - strand, within ABIE40_RS10355 at 1995.783 kb on + strand, within ABIE40_RS10355 at 1995.954 kb on + strand, within ABIE40_RS10355 at 1995.963 kb on + strand, within ABIE40_RS10355 at 1995.963 kb on + strand, within ABIE40_RS10355 at 1995.963 kb on + strand, within ABIE40_RS10355 at 1995.964 kb on - strand, within ABIE40_RS10355 at 1995.964 kb on - strand, within ABIE40_RS10355 at 1995.964 kb on - strand, within ABIE40_RS10355 at 1995.964 kb on - strand, within ABIE40_RS10355 at 1995.964 kb on - strand, within ABIE40_RS10355 at 1995.964 kb on - strand, within ABIE40_RS10355 at 1995.965 kb on + strand, within ABIE40_RS10355 at 1995.965 kb on + strand, within ABIE40_RS10355 at 1995.965 kb on + strand, within ABIE40_RS10355 at 1995.965 kb on + strand, within ABIE40_RS10355 at 1995.965 kb on + strand, within ABIE40_RS10355 at 1995.965 kb on + strand, within ABIE40_RS10355 at 1995.965 kb on + strand, within ABIE40_RS10355 at 1995.965 kb on + strand, within ABIE40_RS10355 at 1995.965 kb on + strand, within ABIE40_RS10355 at 1995.965 kb on + strand, within ABIE40_RS10355 at 1995.965 kb on + strand, within ABIE40_RS10355 at 1995.966 kb on - strand, within ABIE40_RS10355 at 1995.966 kb on - strand, within ABIE40_RS10355 at 1995.966 kb on - strand, within ABIE40_RS10355 at 1995.966 kb on - strand, within ABIE40_RS10355 at 1995.966 kb on - strand, within ABIE40_RS10355 at 1995.966 kb on - strand, within ABIE40_RS10355 at 1995.966 kb on - strand, within ABIE40_RS10355 at 1995.966 kb on - strand, within ABIE40_RS10355 at 1995.966 kb on - strand, within ABIE40_RS10355 at 1995.966 kb on - strand, within ABIE40_RS10355 at 1995.966 kb on - strand, within ABIE40_RS10355 at 1996.593 kb on - strand at 1996.701 kb on - strand at 1996.825 kb on + strand, within ABIE40_RS10360 at 1996.979 kb on + strand, within ABIE40_RS10360 at 1996.990 kb on - strand, within ABIE40_RS10360 at 1997.366 kb on + strand, within ABIE40_RS10360 at 1997.366 kb on + strand, within ABIE40_RS10360 at 1997.367 kb on - strand, within ABIE40_RS10360 at 1997.367 kb on - strand, within ABIE40_RS10360 at 1997.375 kb on + strand, within ABIE40_RS10360 at 1997.414 kb on + strand, within ABIE40_RS10360 at 1997.415 kb on - strand, within ABIE40_RS10360
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with 1-octen-3-ol 0.904549 mM remove 1,994,195 - ABIE40_RS10345 0.67 -0.9 1,994,195 - ABIE40_RS10345 0.67 -0.8 1,994,195 - ABIE40_RS10345 0.67 -0.8 1,994,195 - ABIE40_RS10345 0.67 +3.1 1,994,215 - ABIE40_RS10345 0.72 +1.0 1,994,215 - ABIE40_RS10345 0.72 +3.3 1,994,256 - ABIE40_RS10345 0.82 -0.4 1,994,303 + -0.0 1,994,304 - +0.8 1,994,369 + +0.4 1,994,369 + -0.3 1,994,370 - +0.8 1,994,370 - +0.1 1,994,370 - +0.5 1,994,370 - +0.1 1,994,370 - +0.5 1,994,370 - +0.6 1,994,412 + +1.0 1,994,606 + +0.1 1,994,607 - +2.7 1,994,640 + +1.7 1,994,641 - -0.3 1,994,718 + ABIE40_RS10350 0.22 -0.6 1,994,860 - ABIE40_RS10350 0.51 -2.0 1,994,874 + ABIE40_RS10350 0.54 -0.8 1,994,874 + ABIE40_RS10350 0.54 -2.0 1,994,874 + ABIE40_RS10350 0.54 +0.4 1,994,875 - ABIE40_RS10350 0.54 -0.0 1,994,895 + ABIE40_RS10350 0.58 +0.8 1,994,896 - ABIE40_RS10350 0.58 +1.0 1,994,896 - ABIE40_RS10350 0.58 -0.0 1,994,896 - ABIE40_RS10350 0.58 -0.8 1,994,896 - ABIE40_RS10350 0.58 -0.3 1,994,948 + ABIE40_RS10350 0.69 -0.0 1,995,005 + ABIE40_RS10350 0.80 -1.3 1,995,134 + +2.0 1,995,134 + +2.0 1,995,134 + -2.2 1,995,134 + +1.0 1,995,134 + -0.7 1,995,134 + +0.4 1,995,134 + -3.4 1,995,134 + -1.5 1,995,135 - +0.3 1,995,135 - +0.4 1,995,135 - +1.2 1,995,135 - -1.2 1,995,135 - -1.2 1,995,135 - -1.5 1,995,135 - -0.3 1,995,135 - -0.9 1,995,135 - +0.7 1,995,135 - +0.7 1,995,135 - +0.4 1,995,135 - -0.6 1,995,136 + +3.6 1,995,137 - +1.0 1,995,137 - +0.5 1,995,137 - -0.0 1,995,137 - +0.5 1,995,519 + ABIE40_RS10355 0.24 +0.8 1,995,519 + ABIE40_RS10355 0.24 -0.2 1,995,585 + ABIE40_RS10355 0.28 -0.3 1,995,585 + ABIE40_RS10355 0.28 -0.5 1,995,636 - ABIE40_RS10355 0.32 -0.4 1,995,650 + ABIE40_RS10355 0.33 -1.9 1,995,650 + ABIE40_RS10355 0.33 +0.4 1,995,650 + ABIE40_RS10355 0.33 -1.3 1,995,650 + ABIE40_RS10355 0.33 +0.4 1,995,651 - ABIE40_RS10355 0.33 -1.4 1,995,783 + ABIE40_RS10355 0.42 -1.0 1,995,954 + ABIE40_RS10355 0.53 -1.5 1,995,963 + ABIE40_RS10355 0.54 -0.7 1,995,963 + ABIE40_RS10355 0.54 -0.7 1,995,963 + ABIE40_RS10355 0.54 -1.0 1,995,964 - ABIE40_RS10355 0.54 -0.0 1,995,964 - ABIE40_RS10355 0.54 -1.0 1,995,964 - ABIE40_RS10355 0.54 +0.0 1,995,964 - ABIE40_RS10355 0.54 -1.6 1,995,964 - ABIE40_RS10355 0.54 +0.4 1,995,964 - ABIE40_RS10355 0.54 -1.9 1,995,965 + ABIE40_RS10355 0.54 +0.1 1,995,965 + ABIE40_RS10355 0.54 +0.2 1,995,965 + ABIE40_RS10355 0.54 +0.5 1,995,965 + ABIE40_RS10355 0.54 -0.3 1,995,965 + ABIE40_RS10355 0.54 +0.4 1,995,965 + ABIE40_RS10355 0.54 -0.2 1,995,965 + ABIE40_RS10355 0.54 -0.3 1,995,965 + ABIE40_RS10355 0.54 -0.3 1,995,965 + ABIE40_RS10355 0.54 +0.5 1,995,965 + ABIE40_RS10355 0.54 -1.5 1,995,965 + ABIE40_RS10355 0.54 -0.1 1,995,966 - ABIE40_RS10355 0.54 +0.3 1,995,966 - ABIE40_RS10355 0.54 +2.7 1,995,966 - ABIE40_RS10355 0.54 -0.6 1,995,966 - ABIE40_RS10355 0.54 -0.0 1,995,966 - ABIE40_RS10355 0.54 +1.2 1,995,966 - ABIE40_RS10355 0.54 -1.0 1,995,966 - ABIE40_RS10355 0.54 -0.5 1,995,966 - ABIE40_RS10355 0.54 +0.2 1,995,966 - ABIE40_RS10355 0.54 +1.0 1,995,966 - ABIE40_RS10355 0.54 +0.6 1,995,966 - ABIE40_RS10355 0.54 -0.4 1,996,593 - +1.1 1,996,701 - +1.2 1,996,825 + ABIE40_RS10360 0.17 +0.5 1,996,979 + ABIE40_RS10360 0.31 -1.2 1,996,990 - ABIE40_RS10360 0.32 +0.3 1,997,366 + ABIE40_RS10360 0.67 +0.4 1,997,366 + ABIE40_RS10360 0.67 -0.3 1,997,367 - ABIE40_RS10360 0.67 -0.2 1,997,367 - ABIE40_RS10360 0.67 +1.6 1,997,375 + ABIE40_RS10360 0.68 +0.6 1,997,414 + ABIE40_RS10360 0.71 -0.1 1,997,415 - ABIE40_RS10360 0.72 -0.4
Or see this region's nucleotide sequence