Experiment: R2A with 1-octen-3-ol 0.904549 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE40_RS01775 and ABIE40_RS01780 are separated by 293 nucleotides ABIE40_RS01780 and ABIE40_RS01785 are separated by 36 nucleotides ABIE40_RS01785 and ABIE40_RS01790 overlap by 4 nucleotides ABIE40_RS01790 and ABIE40_RS01795 are separated by 53 nucleotides
ABIE40_RS01775: ABIE40_RS01775 - DNA mismatch repair protein MutS, at 349,426 to 352,152
_RS01775
ABIE40_RS01780: ABIE40_RS01780 - NADP-dependent malic enzyme, at 352,446 to 354,731
_RS01780
ABIE40_RS01785: ABIE40_RS01785 - type II toxin-antitoxin system RelE/ParE family toxin, at 354,768 to 355,058
_RS01785
ABIE40_RS01790: ABIE40_RS01790 - type II toxin-antitoxin system RelB/DinJ family antitoxin, at 355,055 to 355,360
_RS01790
ABIE40_RS01795: ABIE40_RS01795 - GNAT family N-acetyltransferase, at 355,414 to 356,289
_RS01795
Position (kb) 352
353
354
355 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 351.524 kb on + strand, within ABIE40_RS01775 at 351.524 kb on + strand, within ABIE40_RS01775 at 351.524 kb on + strand, within ABIE40_RS01775 at 351.524 kb on + strand, within ABIE40_RS01775 at 351.524 kb on + strand, within ABIE40_RS01775 at 351.524 kb on + strand, within ABIE40_RS01775 at 351.525 kb on - strand, within ABIE40_RS01775 at 351.525 kb on - strand, within ABIE40_RS01775 at 351.525 kb on - strand, within ABIE40_RS01775 at 351.525 kb on - strand, within ABIE40_RS01775 at 351.525 kb on - strand, within ABIE40_RS01775 at 351.526 kb on - strand, within ABIE40_RS01775 at 351.695 kb on + strand, within ABIE40_RS01775 at 351.695 kb on + strand, within ABIE40_RS01775 at 351.695 kb on + strand, within ABIE40_RS01775 at 351.695 kb on + strand, within ABIE40_RS01775 at 351.695 kb on + strand, within ABIE40_RS01775 at 351.695 kb on + strand, within ABIE40_RS01775 at 351.696 kb on - strand, within ABIE40_RS01775 at 351.696 kb on - strand, within ABIE40_RS01775 at 351.869 kb on + strand, within ABIE40_RS01775 at 351.870 kb on - strand, within ABIE40_RS01775 at 351.870 kb on - strand, within ABIE40_RS01775 at 351.996 kb on - strand at 352.014 kb on - strand at 352.190 kb on + strand at 352.205 kb on - strand at 352.206 kb on + strand at 352.206 kb on + strand at 352.206 kb on + strand at 352.207 kb on - strand at 352.207 kb on - strand at 352.207 kb on - strand at 352.207 kb on - strand at 352.207 kb on - strand at 352.220 kb on + strand at 352.221 kb on - strand at 352.221 kb on - strand at 352.265 kb on + strand at 352.265 kb on + strand at 352.265 kb on + strand at 352.266 kb on - strand at 352.339 kb on - strand at 352.345 kb on - strand at 352.389 kb on + strand at 352.390 kb on - strand at 352.390 kb on - strand at 352.391 kb on + strand at 352.391 kb on + strand at 352.391 kb on + strand at 352.391 kb on + strand at 352.391 kb on + strand at 352.391 kb on + strand at 352.391 kb on + strand at 352.392 kb on - strand at 352.392 kb on - strand at 352.392 kb on - strand at 352.392 kb on - strand at 352.392 kb on - strand at 352.445 kb on + strand at 352.445 kb on + strand at 352.445 kb on + strand at 352.445 kb on + strand at 352.445 kb on + strand at 352.446 kb on - strand at 352.446 kb on - strand at 352.446 kb on - strand at 352.446 kb on - strand at 352.446 kb on - strand at 352.446 kb on - strand at 352.446 kb on - strand at 352.590 kb on + strand at 352.591 kb on - strand at 352.591 kb on - strand at 352.647 kb on + strand at 352.653 kb on + strand at 352.653 kb on + strand at 352.653 kb on + strand at 352.886 kb on + strand, within ABIE40_RS01780 at 352.886 kb on + strand, within ABIE40_RS01780 at 352.886 kb on + strand, within ABIE40_RS01780 at 352.887 kb on - strand, within ABIE40_RS01780 at 352.940 kb on + strand, within ABIE40_RS01780 at 353.120 kb on + strand, within ABIE40_RS01780 at 353.120 kb on + strand, within ABIE40_RS01780 at 353.121 kb on - strand, within ABIE40_RS01780 at 353.121 kb on - strand, within ABIE40_RS01780 at 353.151 kb on + strand, within ABIE40_RS01780 at 353.151 kb on + strand, within ABIE40_RS01780 at 353.151 kb on + strand, within ABIE40_RS01780 at 353.151 kb on + strand, within ABIE40_RS01780 at 353.151 kb on + strand, within ABIE40_RS01780 at 353.151 kb on + strand, within ABIE40_RS01780 at 353.151 kb on + strand, within ABIE40_RS01780 at 353.152 kb on - strand, within ABIE40_RS01780 at 353.412 kb on + strand, within ABIE40_RS01780 at 353.448 kb on + strand, within ABIE40_RS01780 at 353.448 kb on + strand, within ABIE40_RS01780 at 353.449 kb on - strand, within ABIE40_RS01780 at 353.449 kb on - strand, within ABIE40_RS01780 at 353.449 kb on - strand, within ABIE40_RS01780 at 353.450 kb on + strand, within ABIE40_RS01780 at 353.450 kb on + strand, within ABIE40_RS01780 at 353.450 kb on + strand, within ABIE40_RS01780 at 353.450 kb on + strand, within ABIE40_RS01780 at 353.450 kb on + strand, within ABIE40_RS01780 at 353.450 kb on + strand, within ABIE40_RS01780 at 353.450 kb on + strand, within ABIE40_RS01780 at 353.451 kb on - strand, within ABIE40_RS01780 at 353.451 kb on - strand, within ABIE40_RS01780 at 353.604 kb on + strand, within ABIE40_RS01780 at 353.604 kb on + strand, within ABIE40_RS01780 at 353.604 kb on + strand, within ABIE40_RS01780 at 353.605 kb on - strand, within ABIE40_RS01780 at 353.605 kb on - strand, within ABIE40_RS01780 at 353.605 kb on - strand, within ABIE40_RS01780 at 353.605 kb on - strand, within ABIE40_RS01780 at 353.605 kb on - strand, within ABIE40_RS01780 at 353.635 kb on + strand, within ABIE40_RS01780 at 353.635 kb on + strand, within ABIE40_RS01780 at 353.636 kb on - strand, within ABIE40_RS01780 at 353.709 kb on - strand, within ABIE40_RS01780 at 353.775 kb on + strand, within ABIE40_RS01780 at 353.856 kb on + strand, within ABIE40_RS01780 at 353.856 kb on + strand, within ABIE40_RS01780 at 353.857 kb on - strand, within ABIE40_RS01780 at 353.857 kb on - strand, within ABIE40_RS01780 at 353.857 kb on - strand, within ABIE40_RS01780 at 353.857 kb on - strand, within ABIE40_RS01780 at 353.857 kb on - strand, within ABIE40_RS01780 at 353.997 kb on + strand, within ABIE40_RS01780 at 353.997 kb on + strand, within ABIE40_RS01780 at 353.997 kb on + strand, within ABIE40_RS01780 at 353.998 kb on - strand, within ABIE40_RS01780 at 353.998 kb on - strand, within ABIE40_RS01780 at 353.998 kb on - strand, within ABIE40_RS01780 at 353.998 kb on - strand, within ABIE40_RS01780 at 353.998 kb on - strand, within ABIE40_RS01780 at 353.998 kb on - strand, within ABIE40_RS01780 at 353.998 kb on - strand, within ABIE40_RS01780 at 353.998 kb on - strand, within ABIE40_RS01780 at 353.998 kb on - strand, within ABIE40_RS01780 at 353.999 kb on + strand, within ABIE40_RS01780 at 353.999 kb on + strand, within ABIE40_RS01780 at 353.999 kb on + strand, within ABIE40_RS01780 at 354.000 kb on - strand, within ABIE40_RS01780 at 354.000 kb on - strand, within ABIE40_RS01780 at 354.000 kb on - strand, within ABIE40_RS01780 at 354.000 kb on - strand, within ABIE40_RS01780 at 354.006 kb on + strand, within ABIE40_RS01780 at 354.007 kb on - strand, within ABIE40_RS01780 at 354.007 kb on - strand, within ABIE40_RS01780 at 354.007 kb on - strand, within ABIE40_RS01780 at 354.007 kb on - strand, within ABIE40_RS01780 at 354.007 kb on - strand, within ABIE40_RS01780 at 354.010 kb on + strand, within ABIE40_RS01780 at 354.012 kb on + strand, within ABIE40_RS01780 at 354.013 kb on - strand, within ABIE40_RS01780 at 354.037 kb on - strand, within ABIE40_RS01780 at 354.037 kb on - strand, within ABIE40_RS01780 at 354.147 kb on + strand, within ABIE40_RS01780 at 354.366 kb on + strand, within ABIE40_RS01780 at 354.366 kb on + strand, within ABIE40_RS01780 at 354.366 kb on + strand, within ABIE40_RS01780 at 354.456 kb on + strand, within ABIE40_RS01780 at 354.510 kb on + strand at 354.714 kb on + strand at 354.714 kb on + strand at 354.715 kb on - strand at 354.715 kb on - strand at 354.715 kb on - strand at 354.729 kb on + strand at 354.729 kb on + strand at 354.730 kb on - strand at 354.770 kb on - strand at 354.820 kb on - strand, within ABIE40_RS01785 at 354.847 kb on + strand, within ABIE40_RS01785 at 354.847 kb on + strand, within ABIE40_RS01785 at 354.847 kb on + strand, within ABIE40_RS01785 at 354.847 kb on + strand, within ABIE40_RS01785 at 354.847 kb on + strand, within ABIE40_RS01785 at 354.848 kb on - strand, within ABIE40_RS01785 at 354.848 kb on - strand, within ABIE40_RS01785 at 354.848 kb on - strand, within ABIE40_RS01785 at 354.848 kb on - strand, within ABIE40_RS01785 at 354.848 kb on - strand, within ABIE40_RS01785 at 354.848 kb on - strand, within ABIE40_RS01785 at 354.859 kb on + strand, within ABIE40_RS01785 at 354.859 kb on + strand, within ABIE40_RS01785 at 354.859 kb on + strand, within ABIE40_RS01785 at 354.859 kb on + strand, within ABIE40_RS01785 at 354.859 kb on + strand, within ABIE40_RS01785 at 354.860 kb on - strand, within ABIE40_RS01785 at 354.860 kb on - strand, within ABIE40_RS01785 at 355.036 kb on + strand at 355.131 kb on - strand, within ABIE40_RS01790 at 355.186 kb on + strand, within ABIE40_RS01790 at 355.187 kb on - strand, within ABIE40_RS01790 at 355.220 kb on + strand, within ABIE40_RS01790 at 355.221 kb on - strand, within ABIE40_RS01790 at 355.387 kb on + strand at 355.415 kb on + strand at 355.415 kb on + strand at 355.415 kb on + strand at 355.415 kb on + strand at 355.416 kb on - strand at 355.416 kb on - strand at 355.416 kb on - strand at 355.416 kb on - strand at 355.419 kb on + strand at 355.420 kb on - strand at 355.420 kb on - strand at 355.420 kb on - strand at 355.445 kb on + strand at 355.445 kb on + strand at 355.446 kb on - strand at 355.446 kb on - strand at 355.446 kb on - strand at 355.448 kb on + strand at 355.448 kb on + strand at 355.449 kb on - strand at 355.449 kb on - strand at 355.458 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with 1-octen-3-ol 0.904549 mM remove 351,524 + ABIE40_RS01775 0.77 +0.1 351,524 + ABIE40_RS01775 0.77 +0.8 351,524 + ABIE40_RS01775 0.77 +0.6 351,524 + ABIE40_RS01775 0.77 +0.1 351,524 + ABIE40_RS01775 0.77 -1.4 351,524 + ABIE40_RS01775 0.77 -0.0 351,525 - ABIE40_RS01775 0.77 -0.2 351,525 - ABIE40_RS01775 0.77 +0.6 351,525 - ABIE40_RS01775 0.77 -1.0 351,525 - ABIE40_RS01775 0.77 +0.0 351,525 - ABIE40_RS01775 0.77 +0.4 351,526 - ABIE40_RS01775 0.77 +0.7 351,695 + ABIE40_RS01775 0.83 -1.1 351,695 + ABIE40_RS01775 0.83 +0.3 351,695 + ABIE40_RS01775 0.83 -1.6 351,695 + ABIE40_RS01775 0.83 -1.3 351,695 + ABIE40_RS01775 0.83 -0.6 351,695 + ABIE40_RS01775 0.83 +0.4 351,696 - ABIE40_RS01775 0.83 +0.3 351,696 - ABIE40_RS01775 0.83 -0.4 351,869 + ABIE40_RS01775 0.90 +2.0 351,870 - ABIE40_RS01775 0.90 +0.4 351,870 - ABIE40_RS01775 0.90 +1.1 351,996 - +1.5 352,014 - -0.4 352,190 + -0.5 352,205 - -0.7 352,206 + +0.3 352,206 + -1.6 352,206 + +0.1 352,207 - +0.4 352,207 - -1.1 352,207 - +0.3 352,207 - +0.9 352,207 - +1.0 352,220 + -0.6 352,221 - -0.2 352,221 - +0.9 352,265 + +0.3 352,265 + -1.1 352,265 + +1.6 352,266 - -2.5 352,339 - -0.7 352,345 - -0.7 352,389 + +0.3 352,390 - -2.2 352,390 - +0.7 352,391 + +1.9 352,391 + +1.0 352,391 + +0.3 352,391 + +0.5 352,391 + -0.6 352,391 + +0.9 352,391 + +1.3 352,392 - -1.3 352,392 - -2.1 352,392 - -4.0 352,392 - -0.5 352,392 - +0.1 352,445 + +0.7 352,445 + -0.6 352,445 + +2.5 352,445 + -0.3 352,445 + +0.1 352,446 - -0.4 352,446 - -0.1 352,446 - -0.7 352,446 - +0.7 352,446 - +1.7 352,446 - +0.1 352,446 - -1.4 352,590 + -1.5 352,591 - -0.2 352,591 - +0.7 352,647 + +0.4 352,653 + -0.0 352,653 + -2.0 352,653 + -1.5 352,886 + ABIE40_RS01780 0.19 -1.6 352,886 + ABIE40_RS01780 0.19 +0.2 352,886 + ABIE40_RS01780 0.19 -1.0 352,887 - ABIE40_RS01780 0.19 -1.5 352,940 + ABIE40_RS01780 0.22 +0.1 353,120 + ABIE40_RS01780 0.29 +0.4 353,120 + ABIE40_RS01780 0.29 +1.1 353,121 - ABIE40_RS01780 0.30 +1.4 353,121 - ABIE40_RS01780 0.30 -0.8 353,151 + ABIE40_RS01780 0.31 +0.4 353,151 + ABIE40_RS01780 0.31 +0.4 353,151 + ABIE40_RS01780 0.31 -1.3 353,151 + ABIE40_RS01780 0.31 -0.4 353,151 + ABIE40_RS01780 0.31 -0.2 353,151 + ABIE40_RS01780 0.31 -0.4 353,151 + ABIE40_RS01780 0.31 -1.6 353,152 - ABIE40_RS01780 0.31 +0.6 353,412 + ABIE40_RS01780 0.42 -0.6 353,448 + ABIE40_RS01780 0.44 -1.4 353,448 + ABIE40_RS01780 0.44 -2.5 353,449 - ABIE40_RS01780 0.44 -0.3 353,449 - ABIE40_RS01780 0.44 +1.4 353,449 - ABIE40_RS01780 0.44 +0.2 353,450 + ABIE40_RS01780 0.44 -0.3 353,450 + ABIE40_RS01780 0.44 +0.3 353,450 + ABIE40_RS01780 0.44 -1.9 353,450 + ABIE40_RS01780 0.44 +0.1 353,450 + ABIE40_RS01780 0.44 -0.9 353,450 + ABIE40_RS01780 0.44 -0.2 353,450 + ABIE40_RS01780 0.44 +1.1 353,451 - ABIE40_RS01780 0.44 +1.2 353,451 - ABIE40_RS01780 0.44 +0.1 353,604 + ABIE40_RS01780 0.51 +1.2 353,604 + ABIE40_RS01780 0.51 -0.9 353,604 + ABIE40_RS01780 0.51 -0.6 353,605 - ABIE40_RS01780 0.51 -1.0 353,605 - ABIE40_RS01780 0.51 +0.5 353,605 - ABIE40_RS01780 0.51 +2.1 353,605 - ABIE40_RS01780 0.51 -0.3 353,605 - ABIE40_RS01780 0.51 +0.7 353,635 + ABIE40_RS01780 0.52 +1.4 353,635 + ABIE40_RS01780 0.52 +0.1 353,636 - ABIE40_RS01780 0.52 -0.4 353,709 - ABIE40_RS01780 0.55 -1.2 353,775 + ABIE40_RS01780 0.58 -3.3 353,856 + ABIE40_RS01780 0.62 -0.1 353,856 + ABIE40_RS01780 0.62 -0.5 353,857 - ABIE40_RS01780 0.62 -2.7 353,857 - ABIE40_RS01780 0.62 -0.2 353,857 - ABIE40_RS01780 0.62 -0.2 353,857 - ABIE40_RS01780 0.62 +1.3 353,857 - ABIE40_RS01780 0.62 +0.0 353,997 + ABIE40_RS01780 0.68 +1.7 353,997 + ABIE40_RS01780 0.68 +1.9 353,997 + ABIE40_RS01780 0.68 -1.1 353,998 - ABIE40_RS01780 0.68 +0.8 353,998 - ABIE40_RS01780 0.68 +1.0 353,998 - ABIE40_RS01780 0.68 +0.4 353,998 - ABIE40_RS01780 0.68 -1.0 353,998 - ABIE40_RS01780 0.68 +0.2 353,998 - ABIE40_RS01780 0.68 +0.7 353,998 - ABIE40_RS01780 0.68 +0.6 353,998 - ABIE40_RS01780 0.68 -1.1 353,998 - ABIE40_RS01780 0.68 +0.6 353,999 + ABIE40_RS01780 0.68 +0.9 353,999 + ABIE40_RS01780 0.68 -0.7 353,999 + ABIE40_RS01780 0.68 +1.6 354,000 - ABIE40_RS01780 0.68 +0.9 354,000 - ABIE40_RS01780 0.68 +0.3 354,000 - ABIE40_RS01780 0.68 +0.7 354,000 - ABIE40_RS01780 0.68 -1.2 354,006 + ABIE40_RS01780 0.68 +0.2 354,007 - ABIE40_RS01780 0.68 -0.0 354,007 - ABIE40_RS01780 0.68 -1.3 354,007 - ABIE40_RS01780 0.68 -0.1 354,007 - ABIE40_RS01780 0.68 +1.5 354,007 - ABIE40_RS01780 0.68 +0.5 354,010 + ABIE40_RS01780 0.68 -0.2 354,012 + ABIE40_RS01780 0.69 +0.7 354,013 - ABIE40_RS01780 0.69 +0.4 354,037 - ABIE40_RS01780 0.70 +1.5 354,037 - ABIE40_RS01780 0.70 +0.1 354,147 + ABIE40_RS01780 0.74 -0.7 354,366 + ABIE40_RS01780 0.84 +0.6 354,366 + ABIE40_RS01780 0.84 +0.7 354,366 + ABIE40_RS01780 0.84 +1.2 354,456 + ABIE40_RS01780 0.88 +0.3 354,510 + -0.1 354,714 + +0.6 354,714 + +0.3 354,715 - -1.0 354,715 - +0.6 354,715 - +0.2 354,729 + +0.3 354,729 + -0.2 354,730 - -0.4 354,770 - +0.8 354,820 - ABIE40_RS01785 0.18 +0.6 354,847 + ABIE40_RS01785 0.27 +2.4 354,847 + ABIE40_RS01785 0.27 +0.1 354,847 + ABIE40_RS01785 0.27 -0.2 354,847 + ABIE40_RS01785 0.27 -2.2 354,847 + ABIE40_RS01785 0.27 -0.4 354,848 - ABIE40_RS01785 0.27 +0.5 354,848 - ABIE40_RS01785 0.27 -0.4 354,848 - ABIE40_RS01785 0.27 +0.3 354,848 - ABIE40_RS01785 0.27 +2.5 354,848 - ABIE40_RS01785 0.27 +0.1 354,848 - ABIE40_RS01785 0.27 -0.3 354,859 + ABIE40_RS01785 0.31 +1.3 354,859 + ABIE40_RS01785 0.31 -0.6 354,859 + ABIE40_RS01785 0.31 +0.4 354,859 + ABIE40_RS01785 0.31 +0.0 354,859 + ABIE40_RS01785 0.31 -0.9 354,860 - ABIE40_RS01785 0.32 +0.1 354,860 - ABIE40_RS01785 0.32 -0.2 355,036 + -0.4 355,131 - ABIE40_RS01790 0.25 -0.6 355,186 + ABIE40_RS01790 0.43 +1.1 355,187 - ABIE40_RS01790 0.43 +0.7 355,220 + ABIE40_RS01790 0.54 -0.0 355,221 - ABIE40_RS01790 0.54 +0.6 355,387 + -1.8 355,415 + -0.2 355,415 + -0.6 355,415 + -0.9 355,415 + -0.6 355,416 - -0.4 355,416 - -0.1 355,416 - +0.1 355,416 - -0.5 355,419 + -0.0 355,420 - +0.5 355,420 - +0.3 355,420 - +0.0 355,445 + -1.1 355,445 + -0.1 355,446 - +1.2 355,446 - +1.0 355,446 - +0.3 355,448 + -0.6 355,448 + -2.3 355,449 - -0.6 355,449 - -0.6 355,458 - +0.3
Or see this region's nucleotide sequence