Experiment: R2A with 1-octen-3-ol 0.904549 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE40_RS14700 and ABIE40_RS14705 overlap by 1 nucleotides ABIE40_RS14705 and ABIE40_RS14710 overlap by 1 nucleotides ABIE40_RS14710 and ABIE40_RS14715 are separated by 5 nucleotides
ABIE40_RS14700: ABIE40_RS14700 - ABC transporter ATP-binding protein, at 2,880,235 to 2,880,963
_RS14700
ABIE40_RS14705: ABIE40_RS14705 - ABC transporter ATP-binding protein, at 2,880,963 to 2,881,826
_RS14705
ABIE40_RS14710: ABIE40_RS14710 - high-affinity branched-chain amino acid ABC transporter permease LivM, at 2,881,826 to 2,883,217
_RS14710
ABIE40_RS14715: ABIE40_RS14715 - branched-chain amino acid ABC transporter permease LivH, at 2,883,223 to 2,884,125
_RS14715
Position (kb)
2881
2882
2883
2884 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 2880.909 kb on + strand at 2880.917 kb on + strand at 2880.920 kb on + strand at 2880.920 kb on + strand at 2880.920 kb on + strand at 2880.920 kb on + strand at 2880.920 kb on + strand at 2880.920 kb on + strand at 2880.921 kb on - strand at 2880.921 kb on - strand at 2880.921 kb on - strand at 2880.921 kb on - strand at 2880.921 kb on - strand at 2880.921 kb on - strand at 2880.981 kb on + strand at 2880.981 kb on + strand at 2880.981 kb on + strand at 2880.982 kb on - strand at 2881.027 kb on + strand at 2881.094 kb on - strand, within ABIE40_RS14705 at 2881.312 kb on + strand, within ABIE40_RS14705 at 2881.444 kb on + strand, within ABIE40_RS14705 at 2881.536 kb on - strand, within ABIE40_RS14705 at 2881.591 kb on + strand, within ABIE40_RS14705 at 2881.591 kb on + strand, within ABIE40_RS14705 at 2881.591 kb on + strand, within ABIE40_RS14705 at 2881.591 kb on + strand, within ABIE40_RS14705 at 2881.591 kb on + strand, within ABIE40_RS14705 at 2881.592 kb on - strand, within ABIE40_RS14705 at 2881.592 kb on - strand, within ABIE40_RS14705 at 2881.592 kb on - strand, within ABIE40_RS14705 at 2881.642 kb on + strand, within ABIE40_RS14705 at 2881.642 kb on + strand, within ABIE40_RS14705 at 2881.643 kb on - strand, within ABIE40_RS14705 at 2881.643 kb on - strand, within ABIE40_RS14705 at 2881.709 kb on + strand, within ABIE40_RS14705 at 2881.709 kb on + strand, within ABIE40_RS14705 at 2881.796 kb on + strand at 2881.796 kb on + strand at 2881.796 kb on + strand at 2881.797 kb on - strand at 2881.797 kb on - strand at 2881.815 kb on + strand at 2881.816 kb on - strand at 2881.905 kb on - strand at 2882.331 kb on + strand, within ABIE40_RS14710 at 2882.331 kb on + strand, within ABIE40_RS14710 at 2882.331 kb on + strand, within ABIE40_RS14710 at 2882.332 kb on - strand, within ABIE40_RS14710 at 2882.332 kb on - strand, within ABIE40_RS14710 at 2882.332 kb on - strand, within ABIE40_RS14710 at 2882.332 kb on - strand, within ABIE40_RS14710 at 2882.332 kb on - strand, within ABIE40_RS14710 at 2882.364 kb on + strand, within ABIE40_RS14710 at 2882.365 kb on - strand, within ABIE40_RS14710 at 2882.365 kb on - strand, within ABIE40_RS14710 at 2882.365 kb on - strand, within ABIE40_RS14710 at 2882.365 kb on - strand, within ABIE40_RS14710 at 2882.382 kb on + strand, within ABIE40_RS14710 at 2882.383 kb on - strand, within ABIE40_RS14710 at 2882.383 kb on - strand, within ABIE40_RS14710 at 2882.383 kb on - strand, within ABIE40_RS14710 at 2882.385 kb on + strand, within ABIE40_RS14710 at 2882.473 kb on + strand, within ABIE40_RS14710 at 2882.482 kb on + strand, within ABIE40_RS14710 at 2882.491 kb on + strand, within ABIE40_RS14710 at 2882.491 kb on + strand, within ABIE40_RS14710 at 2882.492 kb on - strand, within ABIE40_RS14710 at 2882.492 kb on - strand, within ABIE40_RS14710 at 2882.492 kb on - strand, within ABIE40_RS14710 at 2882.492 kb on - strand, within ABIE40_RS14710 at 2882.568 kb on + strand, within ABIE40_RS14710 at 2882.670 kb on + strand, within ABIE40_RS14710 at 2882.671 kb on - strand, within ABIE40_RS14710 at 2882.688 kb on + strand, within ABIE40_RS14710 at 2882.689 kb on - strand, within ABIE40_RS14710 at 2882.709 kb on + strand, within ABIE40_RS14710 at 2882.721 kb on + strand, within ABIE40_RS14710 at 2882.722 kb on - strand, within ABIE40_RS14710 at 2882.784 kb on + strand, within ABIE40_RS14710 at 2882.784 kb on + strand, within ABIE40_RS14710 at 2882.784 kb on + strand, within ABIE40_RS14710 at 2882.784 kb on + strand, within ABIE40_RS14710 at 2882.784 kb on + strand, within ABIE40_RS14710 at 2882.785 kb on - strand, within ABIE40_RS14710 at 2882.785 kb on - strand, within ABIE40_RS14710 at 2882.785 kb on - strand, within ABIE40_RS14710 at 2882.850 kb on + strand, within ABIE40_RS14710 at 2882.850 kb on + strand, within ABIE40_RS14710 at 2882.850 kb on + strand, within ABIE40_RS14710 at 2882.850 kb on + strand, within ABIE40_RS14710 at 2882.850 kb on + strand, within ABIE40_RS14710 at 2882.850 kb on + strand, within ABIE40_RS14710 at 2882.850 kb on + strand, within ABIE40_RS14710 at 2882.851 kb on - strand, within ABIE40_RS14710 at 2882.851 kb on - strand, within ABIE40_RS14710 at 2882.851 kb on - strand, within ABIE40_RS14710 at 2882.851 kb on - strand, within ABIE40_RS14710 at 2882.851 kb on - strand, within ABIE40_RS14710 at 2882.852 kb on - strand, within ABIE40_RS14710 at 2882.865 kb on + strand, within ABIE40_RS14710 at 2882.933 kb on - strand, within ABIE40_RS14710 at 2883.077 kb on - strand, within ABIE40_RS14710 at 2883.108 kb on + strand at 2883.108 kb on + strand at 2883.109 kb on - strand at 2883.109 kb on - strand at 2883.109 kb on - strand at 2883.109 kb on - strand at 2883.160 kb on + strand at 2883.224 kb on + strand at 2883.224 kb on + strand at 2883.224 kb on + strand at 2883.225 kb on - strand at 2883.225 kb on - strand at 2883.226 kb on + strand at 2883.226 kb on + strand at 2883.226 kb on + strand at 2883.226 kb on + strand at 2883.226 kb on + strand at 2883.226 kb on + strand at 2883.226 kb on + strand at 2883.227 kb on - strand at 2883.227 kb on - strand at 2883.314 kb on + strand, within ABIE40_RS14715 at 2883.314 kb on + strand, within ABIE40_RS14715 at 2883.315 kb on - strand, within ABIE40_RS14715 at 2883.315 kb on - strand, within ABIE40_RS14715 at 2883.329 kb on + strand, within ABIE40_RS14715 at 2883.330 kb on - strand, within ABIE40_RS14715 at 2883.470 kb on + strand, within ABIE40_RS14715 at 2883.471 kb on - strand, within ABIE40_RS14715 at 2883.473 kb on + strand, within ABIE40_RS14715 at 2883.473 kb on + strand, within ABIE40_RS14715 at 2883.473 kb on + strand, within ABIE40_RS14715 at 2883.473 kb on + strand, within ABIE40_RS14715 at 2883.473 kb on + strand, within ABIE40_RS14715 at 2883.474 kb on - strand, within ABIE40_RS14715 at 2883.474 kb on - strand, within ABIE40_RS14715 at 2883.474 kb on - strand, within ABIE40_RS14715 at 2883.474 kb on - strand, within ABIE40_RS14715 at 2883.474 kb on - strand, within ABIE40_RS14715 at 2883.474 kb on - strand, within ABIE40_RS14715 at 2883.489 kb on + strand, within ABIE40_RS14715 at 2883.619 kb on - strand, within ABIE40_RS14715 at 2883.632 kb on + strand, within ABIE40_RS14715 at 2883.632 kb on + strand, within ABIE40_RS14715 at 2883.633 kb on - strand, within ABIE40_RS14715 at 2883.633 kb on - strand, within ABIE40_RS14715 at 2883.709 kb on - strand, within ABIE40_RS14715 at 2883.712 kb on - strand, within ABIE40_RS14715 at 2883.712 kb on - strand, within ABIE40_RS14715 at 2883.712 kb on - strand, within ABIE40_RS14715 at 2883.713 kb on + strand, within ABIE40_RS14715 at 2883.713 kb on + strand, within ABIE40_RS14715 at 2883.713 kb on + strand, within ABIE40_RS14715 at 2883.714 kb on - strand, within ABIE40_RS14715 at 2883.725 kb on - strand, within ABIE40_RS14715 at 2883.849 kb on - strand, within ABIE40_RS14715 at 2883.849 kb on - strand, within ABIE40_RS14715 at 2883.928 kb on + strand, within ABIE40_RS14715 at 2884.040 kb on + strand at 2884.050 kb on + strand at 2884.050 kb on + strand at 2884.050 kb on + strand at 2884.051 kb on - strand at 2884.051 kb on - strand at 2884.051 kb on - strand at 2884.051 kb on - strand at 2884.051 kb on - strand at 2884.052 kb on + strand at 2884.053 kb on - strand at 2884.073 kb on + strand at 2884.073 kb on + strand at 2884.074 kb on - strand at 2884.074 kb on - strand at 2884.074 kb on - strand at 2884.114 kb on - strand at 2884.118 kb on + strand at 2884.118 kb on + strand at 2884.118 kb on + strand at 2884.118 kb on + strand at 2884.119 kb on - strand at 2884.119 kb on - strand at 2884.119 kb on - strand at 2884.125 kb on + strand at 2884.125 kb on + strand at 2884.125 kb on + strand at 2884.125 kb on + strand at 2884.125 kb on + strand at 2884.125 kb on + strand at 2884.125 kb on + strand at 2884.126 kb on - strand at 2884.126 kb on - strand at 2884.126 kb on - strand at 2884.126 kb on - strand at 2884.126 kb on - strand at 2884.126 kb on - strand at 2884.126 kb on - strand at 2884.126 kb on - strand at 2884.189 kb on + strand at 2884.216 kb on + strand at 2884.217 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with 1-octen-3-ol 0.904549 mM remove 2,880,909 + +1.7 2,880,917 + -0.1 2,880,920 + -0.8 2,880,920 + -0.2 2,880,920 + -0.8 2,880,920 + -1.0 2,880,920 + +1.2 2,880,920 + +0.3 2,880,921 - +0.5 2,880,921 - +0.5 2,880,921 - +0.2 2,880,921 - +0.8 2,880,921 - +0.2 2,880,921 - -0.0 2,880,981 + -1.8 2,880,981 + -0.4 2,880,981 + +0.1 2,880,982 - -0.9 2,881,027 + -1.3 2,881,094 - ABIE40_RS14705 0.15 +0.9 2,881,312 + ABIE40_RS14705 0.40 -0.2 2,881,444 + ABIE40_RS14705 0.56 -1.2 2,881,536 - ABIE40_RS14705 0.66 +1.4 2,881,591 + ABIE40_RS14705 0.73 +0.6 2,881,591 + ABIE40_RS14705 0.73 +1.6 2,881,591 + ABIE40_RS14705 0.73 -0.9 2,881,591 + ABIE40_RS14705 0.73 -0.3 2,881,591 + ABIE40_RS14705 0.73 +0.5 2,881,592 - ABIE40_RS14705 0.73 -0.8 2,881,592 - ABIE40_RS14705 0.73 -0.1 2,881,592 - ABIE40_RS14705 0.73 +2.8 2,881,642 + ABIE40_RS14705 0.79 -1.0 2,881,642 + ABIE40_RS14705 0.79 -0.3 2,881,643 - ABIE40_RS14705 0.79 -2.7 2,881,643 - ABIE40_RS14705 0.79 -0.2 2,881,709 + ABIE40_RS14705 0.86 -0.4 2,881,709 + ABIE40_RS14705 0.86 -1.1 2,881,796 + -0.8 2,881,796 + -0.7 2,881,796 + +2.2 2,881,797 - -1.0 2,881,797 - -2.0 2,881,815 + -1.7 2,881,816 - -1.8 2,881,905 - -0.7 2,882,331 + ABIE40_RS14710 0.36 -2.7 2,882,331 + ABIE40_RS14710 0.36 -2.8 2,882,331 + ABIE40_RS14710 0.36 -1.4 2,882,332 - ABIE40_RS14710 0.36 +0.2 2,882,332 - ABIE40_RS14710 0.36 -0.5 2,882,332 - ABIE40_RS14710 0.36 -0.2 2,882,332 - ABIE40_RS14710 0.36 +0.9 2,882,332 - ABIE40_RS14710 0.36 -0.8 2,882,364 + ABIE40_RS14710 0.39 +0.2 2,882,365 - ABIE40_RS14710 0.39 +1.5 2,882,365 - ABIE40_RS14710 0.39 -1.1 2,882,365 - ABIE40_RS14710 0.39 -0.5 2,882,365 - ABIE40_RS14710 0.39 -3.9 2,882,382 + ABIE40_RS14710 0.40 -0.9 2,882,383 - ABIE40_RS14710 0.40 -1.8 2,882,383 - ABIE40_RS14710 0.40 +0.4 2,882,383 - ABIE40_RS14710 0.40 +0.1 2,882,385 + ABIE40_RS14710 0.40 +1.1 2,882,473 + ABIE40_RS14710 0.46 +1.2 2,882,482 + ABIE40_RS14710 0.47 -1.7 2,882,491 + ABIE40_RS14710 0.48 -1.7 2,882,491 + ABIE40_RS14710 0.48 -0.3 2,882,492 - ABIE40_RS14710 0.48 -0.7 2,882,492 - ABIE40_RS14710 0.48 +0.5 2,882,492 - ABIE40_RS14710 0.48 +0.3 2,882,492 - ABIE40_RS14710 0.48 +0.6 2,882,568 + ABIE40_RS14710 0.53 +0.8 2,882,670 + ABIE40_RS14710 0.61 -0.5 2,882,671 - ABIE40_RS14710 0.61 +0.5 2,882,688 + ABIE40_RS14710 0.62 +0.5 2,882,689 - ABIE40_RS14710 0.62 -1.5 2,882,709 + ABIE40_RS14710 0.63 +0.1 2,882,721 + ABIE40_RS14710 0.64 -0.2 2,882,722 - ABIE40_RS14710 0.64 -1.4 2,882,784 + ABIE40_RS14710 0.69 +0.3 2,882,784 + ABIE40_RS14710 0.69 -1.7 2,882,784 + ABIE40_RS14710 0.69 +0.4 2,882,784 + ABIE40_RS14710 0.69 -2.3 2,882,784 + ABIE40_RS14710 0.69 -0.3 2,882,785 - ABIE40_RS14710 0.69 +0.8 2,882,785 - ABIE40_RS14710 0.69 +0.6 2,882,785 - ABIE40_RS14710 0.69 +2.3 2,882,850 + ABIE40_RS14710 0.74 +0.1 2,882,850 + ABIE40_RS14710 0.74 -2.8 2,882,850 + ABIE40_RS14710 0.74 +3.4 2,882,850 + ABIE40_RS14710 0.74 +0.6 2,882,850 + ABIE40_RS14710 0.74 +2.1 2,882,850 + ABIE40_RS14710 0.74 +0.4 2,882,850 + ABIE40_RS14710 0.74 -0.0 2,882,851 - ABIE40_RS14710 0.74 +1.3 2,882,851 - ABIE40_RS14710 0.74 -0.7 2,882,851 - ABIE40_RS14710 0.74 -2.8 2,882,851 - ABIE40_RS14710 0.74 -0.3 2,882,851 - ABIE40_RS14710 0.74 -1.4 2,882,852 - ABIE40_RS14710 0.74 -0.5 2,882,865 + ABIE40_RS14710 0.75 -1.3 2,882,933 - ABIE40_RS14710 0.80 -3.6 2,883,077 - ABIE40_RS14710 0.90 -1.0 2,883,108 + -1.1 2,883,108 + +0.4 2,883,109 - -0.2 2,883,109 - -2.3 2,883,109 - -3.8 2,883,109 - +0.8 2,883,160 + +0.2 2,883,224 + +0.2 2,883,224 + -1.1 2,883,224 + -1.5 2,883,225 - +0.1 2,883,225 - -1.0 2,883,226 + -0.9 2,883,226 + -3.7 2,883,226 + +0.1 2,883,226 + -1.4 2,883,226 + -1.6 2,883,226 + -0.7 2,883,226 + -0.6 2,883,227 - +0.6 2,883,227 - -1.6 2,883,314 + ABIE40_RS14715 0.10 -0.8 2,883,314 + ABIE40_RS14715 0.10 -0.3 2,883,315 - ABIE40_RS14715 0.10 +0.3 2,883,315 - ABIE40_RS14715 0.10 -1.4 2,883,329 + ABIE40_RS14715 0.12 -1.8 2,883,330 - ABIE40_RS14715 0.12 -0.9 2,883,470 + ABIE40_RS14715 0.27 +1.2 2,883,471 - ABIE40_RS14715 0.27 -0.1 2,883,473 + ABIE40_RS14715 0.28 +0.2 2,883,473 + ABIE40_RS14715 0.28 +0.2 2,883,473 + ABIE40_RS14715 0.28 +1.6 2,883,473 + ABIE40_RS14715 0.28 -1.7 2,883,473 + ABIE40_RS14715 0.28 -2.1 2,883,474 - ABIE40_RS14715 0.28 +0.5 2,883,474 - ABIE40_RS14715 0.28 -1.4 2,883,474 - ABIE40_RS14715 0.28 +0.9 2,883,474 - ABIE40_RS14715 0.28 +0.3 2,883,474 - ABIE40_RS14715 0.28 -0.7 2,883,474 - ABIE40_RS14715 0.28 +1.2 2,883,489 + ABIE40_RS14715 0.29 +0.7 2,883,619 - ABIE40_RS14715 0.44 -0.2 2,883,632 + ABIE40_RS14715 0.45 +0.4 2,883,632 + ABIE40_RS14715 0.45 -0.7 2,883,633 - ABIE40_RS14715 0.45 +0.2 2,883,633 - ABIE40_RS14715 0.45 +1.1 2,883,709 - ABIE40_RS14715 0.54 -0.5 2,883,712 - ABIE40_RS14715 0.54 -1.5 2,883,712 - ABIE40_RS14715 0.54 -0.3 2,883,712 - ABIE40_RS14715 0.54 +0.5 2,883,713 + ABIE40_RS14715 0.54 -0.5 2,883,713 + ABIE40_RS14715 0.54 -1.1 2,883,713 + ABIE40_RS14715 0.54 -0.5 2,883,714 - ABIE40_RS14715 0.54 +0.5 2,883,725 - ABIE40_RS14715 0.56 -2.9 2,883,849 - ABIE40_RS14715 0.69 -0.0 2,883,849 - ABIE40_RS14715 0.69 -3.9 2,883,928 + ABIE40_RS14715 0.78 -1.2 2,884,040 + -1.0 2,884,050 + -2.1 2,884,050 + -0.2 2,884,050 + -2.6 2,884,051 - +0.2 2,884,051 - -1.4 2,884,051 - -1.0 2,884,051 - -1.7 2,884,051 - -0.2 2,884,052 + -0.2 2,884,053 - +0.7 2,884,073 + -2.1 2,884,073 + +1.8 2,884,074 - -0.5 2,884,074 - +0.8 2,884,074 - -2.0 2,884,114 - -0.3 2,884,118 + -1.7 2,884,118 + +0.5 2,884,118 + -2.4 2,884,118 + +0.2 2,884,119 - -1.5 2,884,119 - -0.7 2,884,119 - -3.3 2,884,125 + -2.8 2,884,125 + -0.5 2,884,125 + -1.6 2,884,125 + -1.7 2,884,125 + -0.4 2,884,125 + -0.3 2,884,125 + +0.2 2,884,126 - -1.2 2,884,126 - +0.1 2,884,126 - +0.2 2,884,126 - -2.1 2,884,126 - -2.4 2,884,126 - -0.2 2,884,126 - -0.2 2,884,126 - -2.0 2,884,189 + -2.8 2,884,216 + +0.8 2,884,217 - +0.4
Or see this region's nucleotide sequence