Experiment: R2A with 1-octen-3-ol 0.904549 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE40_RS08355 and ABIE40_RS08360 are separated by 146 nucleotides ABIE40_RS08360 and ABIE40_RS08365 are separated by 56 nucleotides ABIE40_RS08365 and ABIE40_RS08370 are separated by 200 nucleotides ABIE40_RS08370 and ABIE40_RS08375 are separated by 148 nucleotides ABIE40_RS08375 and ABIE40_RS08380 are separated by 29 nucleotides ABIE40_RS08380 and ABIE40_RS08385 are separated by 69 nucleotides
ABIE40_RS08355: ABIE40_RS08355 - DUF47 domain-containing protein, at 1,607,166 to 1,607,807
_RS08355
ABIE40_RS08360: ABIE40_RS08360 - hypothetical protein, at 1,607,954 to 1,608,121
_RS08360
ABIE40_RS08365: ABIE40_RS08365 - hypothetical protein, at 1,608,178 to 1,608,411
_RS08365
ABIE40_RS08370: ABIE40_RS08370 - tRNA-Cys, at 1,608,612 to 1,608,685
_RS08370
ABIE40_RS08375: ABIE40_RS08375 - hypothetical protein, at 1,608,834 to 1,609,067
_RS08375
ABIE40_RS08380: ABIE40_RS08380 - hypothetical protein, at 1,609,097 to 1,609,240
_RS08380
ABIE40_RS08385: ABIE40_RS08385 - hypothetical protein, at 1,609,310 to 1,609,534
_RS08385
Position (kb)
1608
1609 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1607.245 kb on + strand, within ABIE40_RS08355 at 1607.246 kb on - strand, within ABIE40_RS08355 at 1607.246 kb on - strand, within ABIE40_RS08355 at 1607.246 kb on - strand, within ABIE40_RS08355 at 1607.246 kb on - strand, within ABIE40_RS08355 at 1607.246 kb on - strand, within ABIE40_RS08355 at 1607.246 kb on - strand, within ABIE40_RS08355 at 1607.264 kb on + strand, within ABIE40_RS08355 at 1607.264 kb on + strand, within ABIE40_RS08355 at 1607.264 kb on + strand, within ABIE40_RS08355 at 1607.265 kb on - strand, within ABIE40_RS08355 at 1607.265 kb on - strand, within ABIE40_RS08355 at 1607.266 kb on + strand, within ABIE40_RS08355 at 1607.266 kb on + strand, within ABIE40_RS08355 at 1607.266 kb on + strand, within ABIE40_RS08355 at 1607.266 kb on + strand, within ABIE40_RS08355 at 1607.267 kb on - strand, within ABIE40_RS08355 at 1607.267 kb on - strand, within ABIE40_RS08355 at 1607.267 kb on - strand, within ABIE40_RS08355 at 1607.267 kb on - strand, within ABIE40_RS08355 at 1607.267 kb on - strand, within ABIE40_RS08355 at 1607.267 kb on - strand, within ABIE40_RS08355 at 1607.267 kb on - strand, within ABIE40_RS08355 at 1607.267 kb on - strand, within ABIE40_RS08355 at 1607.267 kb on - strand, within ABIE40_RS08355 at 1607.267 kb on - strand, within ABIE40_RS08355 at 1607.267 kb on - strand, within ABIE40_RS08355 at 1607.324 kb on - strand, within ABIE40_RS08355 at 1607.324 kb on - strand, within ABIE40_RS08355 at 1607.324 kb on - strand, within ABIE40_RS08355 at 1607.631 kb on - strand, within ABIE40_RS08355 at 1607.633 kb on + strand, within ABIE40_RS08355 at 1607.633 kb on + strand, within ABIE40_RS08355 at 1607.633 kb on + strand, within ABIE40_RS08355 at 1607.633 kb on + strand, within ABIE40_RS08355 at 1607.634 kb on - strand, within ABIE40_RS08355 at 1607.634 kb on - strand, within ABIE40_RS08355 at 1607.634 kb on - strand, within ABIE40_RS08355 at 1607.802 kb on + strand at 1607.802 kb on + strand at 1607.802 kb on + strand at 1607.802 kb on + strand at 1607.802 kb on + strand at 1607.803 kb on - strand at 1607.803 kb on - strand at 1607.867 kb on + strand at 1608.121 kb on + strand at 1608.122 kb on - strand at 1608.237 kb on - strand, within ABIE40_RS08365 at 1608.261 kb on - strand, within ABIE40_RS08365 at 1608.293 kb on + strand, within ABIE40_RS08365 at 1608.294 kb on - strand, within ABIE40_RS08365 at 1608.360 kb on - strand, within ABIE40_RS08365 at 1608.471 kb on - strand at 1608.545 kb on + strand at 1608.546 kb on - strand at 1608.576 kb on - strand at 1608.825 kb on + strand at 1608.826 kb on - strand at 1608.831 kb on + strand at 1608.831 kb on + strand at 1608.832 kb on - strand at 1608.832 kb on - strand at 1608.832 kb on - strand at 1608.832 kb on - strand at 1608.834 kb on - strand at 1608.834 kb on - strand at 1608.842 kb on + strand at 1608.886 kb on + strand, within ABIE40_RS08375 at 1608.886 kb on + strand, within ABIE40_RS08375 at 1608.886 kb on + strand, within ABIE40_RS08375 at 1608.886 kb on + strand, within ABIE40_RS08375 at 1608.886 kb on + strand, within ABIE40_RS08375 at 1608.886 kb on + strand, within ABIE40_RS08375 at 1608.886 kb on + strand, within ABIE40_RS08375 at 1608.886 kb on + strand, within ABIE40_RS08375 at 1608.886 kb on + strand, within ABIE40_RS08375 at 1608.886 kb on + strand, within ABIE40_RS08375 at 1608.886 kb on + strand, within ABIE40_RS08375 at 1608.886 kb on + strand, within ABIE40_RS08375 at 1608.886 kb on + strand, within ABIE40_RS08375 at 1608.887 kb on - strand, within ABIE40_RS08375 at 1608.887 kb on - strand, within ABIE40_RS08375 at 1608.887 kb on - strand, within ABIE40_RS08375 at 1608.887 kb on - strand, within ABIE40_RS08375 at 1608.887 kb on - strand, within ABIE40_RS08375 at 1608.887 kb on - strand, within ABIE40_RS08375 at 1608.887 kb on - strand, within ABIE40_RS08375 at 1608.887 kb on - strand, within ABIE40_RS08375 at 1608.910 kb on - strand, within ABIE40_RS08375 at 1609.013 kb on + strand, within ABIE40_RS08375 at 1609.013 kb on + strand, within ABIE40_RS08375 at 1609.268 kb on + strand at 1609.269 kb on - strand at 1609.273 kb on + strand at 1609.273 kb on + strand at 1609.273 kb on + strand at 1609.273 kb on + strand at 1609.273 kb on + strand at 1609.273 kb on + strand at 1609.273 kb on + strand at 1609.273 kb on + strand at 1609.273 kb on + strand at 1609.273 kb on + strand at 1609.274 kb on - strand at 1609.274 kb on - strand at 1609.274 kb on - strand at 1609.274 kb on - strand at 1609.336 kb on - strand, within ABIE40_RS08385 at 1609.341 kb on + strand, within ABIE40_RS08385 at 1609.341 kb on + strand, within ABIE40_RS08385 at 1609.341 kb on + strand, within ABIE40_RS08385 at 1609.341 kb on + strand, within ABIE40_RS08385 at 1609.342 kb on - strand, within ABIE40_RS08385 at 1609.342 kb on - strand, within ABIE40_RS08385 at 1609.401 kb on + strand, within ABIE40_RS08385
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with 1-octen-3-ol 0.904549 mM remove 1,607,245 + ABIE40_RS08355 0.12 +0.8 1,607,246 - ABIE40_RS08355 0.12 -1.4 1,607,246 - ABIE40_RS08355 0.12 -0.4 1,607,246 - ABIE40_RS08355 0.12 -0.2 1,607,246 - ABIE40_RS08355 0.12 -1.1 1,607,246 - ABIE40_RS08355 0.12 -0.9 1,607,246 - ABIE40_RS08355 0.12 -0.5 1,607,264 + ABIE40_RS08355 0.15 +0.4 1,607,264 + ABIE40_RS08355 0.15 +0.5 1,607,264 + ABIE40_RS08355 0.15 +0.1 1,607,265 - ABIE40_RS08355 0.15 +1.6 1,607,265 - ABIE40_RS08355 0.15 -1.4 1,607,266 + ABIE40_RS08355 0.16 -0.9 1,607,266 + ABIE40_RS08355 0.16 +0.1 1,607,266 + ABIE40_RS08355 0.16 -2.7 1,607,266 + ABIE40_RS08355 0.16 -1.2 1,607,267 - ABIE40_RS08355 0.16 +0.4 1,607,267 - ABIE40_RS08355 0.16 -0.8 1,607,267 - ABIE40_RS08355 0.16 -0.2 1,607,267 - ABIE40_RS08355 0.16 +0.4 1,607,267 - ABIE40_RS08355 0.16 +0.4 1,607,267 - ABIE40_RS08355 0.16 -1.0 1,607,267 - ABIE40_RS08355 0.16 +0.4 1,607,267 - ABIE40_RS08355 0.16 -1.3 1,607,267 - ABIE40_RS08355 0.16 +0.4 1,607,267 - ABIE40_RS08355 0.16 -1.0 1,607,267 - ABIE40_RS08355 0.16 -0.2 1,607,324 - ABIE40_RS08355 0.25 -2.7 1,607,324 - ABIE40_RS08355 0.25 -0.5 1,607,324 - ABIE40_RS08355 0.25 -0.4 1,607,631 - ABIE40_RS08355 0.72 -0.9 1,607,633 + ABIE40_RS08355 0.73 +0.1 1,607,633 + ABIE40_RS08355 0.73 +1.0 1,607,633 + ABIE40_RS08355 0.73 +0.5 1,607,633 + ABIE40_RS08355 0.73 -0.8 1,607,634 - ABIE40_RS08355 0.73 +1.4 1,607,634 - ABIE40_RS08355 0.73 +0.4 1,607,634 - ABIE40_RS08355 0.73 -0.2 1,607,802 + -2.7 1,607,802 + -0.9 1,607,802 + +1.0 1,607,802 + -1.0 1,607,802 + +0.8 1,607,803 - +0.1 1,607,803 - +0.0 1,607,867 + -0.5 1,608,121 + -0.9 1,608,122 - -0.0 1,608,237 - ABIE40_RS08365 0.25 -1.0 1,608,261 - ABIE40_RS08365 0.35 -1.2 1,608,293 + ABIE40_RS08365 0.49 -3.8 1,608,294 - ABIE40_RS08365 0.50 +1.1 1,608,360 - ABIE40_RS08365 0.78 -1.5 1,608,471 - -2.6 1,608,545 + +2.0 1,608,546 - +1.0 1,608,576 - -3.0 1,608,825 + -0.2 1,608,826 - -0.0 1,608,831 + +1.1 1,608,831 + -0.4 1,608,832 - +0.4 1,608,832 - +1.2 1,608,832 - -0.3 1,608,832 - +0.2 1,608,834 - -0.9 1,608,834 - +0.6 1,608,842 + +0.1 1,608,886 + ABIE40_RS08375 0.22 +0.2 1,608,886 + ABIE40_RS08375 0.22 -0.7 1,608,886 + ABIE40_RS08375 0.22 +0.7 1,608,886 + ABIE40_RS08375 0.22 -1.0 1,608,886 + ABIE40_RS08375 0.22 -1.9 1,608,886 + ABIE40_RS08375 0.22 +0.8 1,608,886 + ABIE40_RS08375 0.22 -1.3 1,608,886 + ABIE40_RS08375 0.22 -0.2 1,608,886 + ABIE40_RS08375 0.22 -0.5 1,608,886 + ABIE40_RS08375 0.22 -0.4 1,608,886 + ABIE40_RS08375 0.22 -0.2 1,608,886 + ABIE40_RS08375 0.22 -0.2 1,608,886 + ABIE40_RS08375 0.22 -0.4 1,608,887 - ABIE40_RS08375 0.23 +1.2 1,608,887 - ABIE40_RS08375 0.23 +0.1 1,608,887 - ABIE40_RS08375 0.23 -1.4 1,608,887 - ABIE40_RS08375 0.23 +0.9 1,608,887 - ABIE40_RS08375 0.23 +2.0 1,608,887 - ABIE40_RS08375 0.23 -1.7 1,608,887 - ABIE40_RS08375 0.23 -2.6 1,608,887 - ABIE40_RS08375 0.23 -0.5 1,608,910 - ABIE40_RS08375 0.32 +0.3 1,609,013 + ABIE40_RS08375 0.76 -1.6 1,609,013 + ABIE40_RS08375 0.76 +1.2 1,609,268 + -0.9 1,609,269 - +1.3 1,609,273 + +0.5 1,609,273 + +1.0 1,609,273 + -0.3 1,609,273 + -0.3 1,609,273 + -1.3 1,609,273 + -0.9 1,609,273 + -1.0 1,609,273 + +0.2 1,609,273 + -3.1 1,609,273 + -1.6 1,609,274 - +0.2 1,609,274 - +0.2 1,609,274 - +0.2 1,609,274 - -0.7 1,609,336 - ABIE40_RS08385 0.12 +0.1 1,609,341 + ABIE40_RS08385 0.14 +0.7 1,609,341 + ABIE40_RS08385 0.14 -1.5 1,609,341 + ABIE40_RS08385 0.14 -0.1 1,609,341 + ABIE40_RS08385 0.14 -0.9 1,609,342 - ABIE40_RS08385 0.14 +0.7 1,609,342 - ABIE40_RS08385 0.14 -0.4 1,609,401 + ABIE40_RS08385 0.40 -1.0
Or see this region's nucleotide sequence