Experiment: R2A with 1-octen-3-ol 0.904549 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE40_RS00355 and ABIE40_RS00360 are separated by 75 nucleotides ABIE40_RS00360 and ABIE40_RS00365 overlap by 26 nucleotides ABIE40_RS00365 and ABIE40_RS00370 are separated by 10 nucleotides ABIE40_RS00370 and ABIE40_RS00375 are separated by 103 nucleotides
ABIE40_RS00355: ABIE40_RS00355 - polyribonucleotide nucleotidyltransferase, at 72,252 to 74,390
_RS00355
ABIE40_RS00360: ABIE40_RS00360 - class I SAM-dependent methyltransferase, at 74,466 to 75,482
_RS00360
ABIE40_RS00365: ABIE40_RS00365 - type II toxin-antitoxin system HicB family antitoxin, at 75,457 to 75,882
_RS00365
ABIE40_RS00370: ABIE40_RS00370 - type II toxin-antitoxin system HicA family toxin, at 75,893 to 76,075
_RS00370
ABIE40_RS00375: ABIE40_RS00375 - enoyl-ACP reductase FabI, at 76,179 to 76,985
_RS00375
Position (kb) 74
75
76 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 74.389 kb on - strand at 74.612 kb on - strand, within ABIE40_RS00360 at 74.612 kb on - strand, within ABIE40_RS00360 at 74.688 kb on + strand, within ABIE40_RS00360 at 74.702 kb on - strand, within ABIE40_RS00360 at 74.741 kb on + strand, within ABIE40_RS00360 at 74.871 kb on + strand, within ABIE40_RS00360 at 74.872 kb on - strand, within ABIE40_RS00360 at 75.000 kb on - strand, within ABIE40_RS00360 at 75.084 kb on + strand, within ABIE40_RS00360 at 75.085 kb on - strand, within ABIE40_RS00360 at 75.138 kb on + strand, within ABIE40_RS00360 at 75.139 kb on - strand, within ABIE40_RS00360 at 75.139 kb on - strand, within ABIE40_RS00360 at 75.255 kb on + strand, within ABIE40_RS00360 at 75.255 kb on + strand, within ABIE40_RS00360 at 75.255 kb on + strand, within ABIE40_RS00360 at 75.255 kb on + strand, within ABIE40_RS00360 at 75.255 kb on + strand, within ABIE40_RS00360 at 75.256 kb on - strand, within ABIE40_RS00360 at 75.256 kb on - strand, within ABIE40_RS00360 at 75.256 kb on - strand, within ABIE40_RS00360 at 75.349 kb on + strand, within ABIE40_RS00360 at 75.391 kb on + strand at 75.392 kb on - strand at 75.392 kb on - strand at 75.396 kb on + strand at 75.396 kb on + strand at 75.396 kb on + strand at 75.397 kb on - strand at 75.397 kb on - strand at 75.481 kb on - strand at 75.481 kb on - strand at 75.488 kb on - strand at 75.583 kb on - strand, within ABIE40_RS00365 at 75.583 kb on - strand, within ABIE40_RS00365 at 75.583 kb on - strand, within ABIE40_RS00365 at 75.583 kb on - strand, within ABIE40_RS00365 at 75.641 kb on - strand, within ABIE40_RS00365 at 75.897 kb on - strand at 75.994 kb on + strand, within ABIE40_RS00370 at 76.041 kb on - strand, within ABIE40_RS00370 at 76.099 kb on - strand at 76.100 kb on + strand at 76.100 kb on + strand at 76.100 kb on + strand at 76.100 kb on + strand at 76.100 kb on + strand at 76.100 kb on + strand at 76.163 kb on + strand at 76.180 kb on + strand at 76.180 kb on + strand at 76.180 kb on + strand at 76.180 kb on + strand at 76.180 kb on + strand at 76.181 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with 1-octen-3-ol 0.904549 mM remove 74,389 - +0.6 74,612 - ABIE40_RS00360 0.14 -0.5 74,612 - ABIE40_RS00360 0.14 +1.0 74,688 + ABIE40_RS00360 0.22 -0.9 74,702 - ABIE40_RS00360 0.23 -0.0 74,741 + ABIE40_RS00360 0.27 +0.1 74,871 + ABIE40_RS00360 0.40 +0.2 74,872 - ABIE40_RS00360 0.40 +0.8 75,000 - ABIE40_RS00360 0.53 +0.5 75,084 + ABIE40_RS00360 0.61 -0.3 75,085 - ABIE40_RS00360 0.61 -0.1 75,138 + ABIE40_RS00360 0.66 -3.1 75,139 - ABIE40_RS00360 0.66 +0.2 75,139 - ABIE40_RS00360 0.66 +0.9 75,255 + ABIE40_RS00360 0.78 -0.1 75,255 + ABIE40_RS00360 0.78 +0.0 75,255 + ABIE40_RS00360 0.78 +0.3 75,255 + ABIE40_RS00360 0.78 +0.4 75,255 + ABIE40_RS00360 0.78 -0.1 75,256 - ABIE40_RS00360 0.78 +0.6 75,256 - ABIE40_RS00360 0.78 -0.8 75,256 - ABIE40_RS00360 0.78 -0.8 75,349 + ABIE40_RS00360 0.87 -0.5 75,391 + -0.6 75,392 - +1.0 75,392 - -1.1 75,396 + +0.4 75,396 + +0.4 75,396 + -1.0 75,397 - -0.5 75,397 - -0.1 75,481 - +1.0 75,481 - +0.8 75,488 - -2.5 75,583 - ABIE40_RS00365 0.30 -3.6 75,583 - ABIE40_RS00365 0.30 +0.4 75,583 - ABIE40_RS00365 0.30 +0.5 75,583 - ABIE40_RS00365 0.30 +0.2 75,641 - ABIE40_RS00365 0.43 -0.4 75,897 - +1.1 75,994 + ABIE40_RS00370 0.55 +1.1 76,041 - ABIE40_RS00370 0.81 -0.7 76,099 - -2.7 76,100 + -2.0 76,100 + -2.3 76,100 + -0.0 76,100 + +2.5 76,100 + -1.5 76,100 + +0.6 76,163 + -0.4 76,180 + -0.2 76,180 + +1.4 76,180 + +0.8 76,180 + +0.1 76,180 + -0.9 76,181 - +0.0
Or see this region's nucleotide sequence