Experiment: R2A with 1-octen-3-ol 1.08545883333333 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE40_RS16070 and ABIE40_RS16075 are separated by 154 nucleotides ABIE40_RS16075 and ABIE40_RS16080 are separated by 111 nucleotides ABIE40_RS16080 and ABIE40_RS16085 are separated by 62 nucleotides
ABIE40_RS16070: ABIE40_RS16070 - 5-(carboxyamino)imidazole ribonucleotide synthase, at 3,182,752 to 3,183,816
_RS16070
ABIE40_RS16075: ABIE40_RS16075 - type B 50S ribosomal protein L36, at 3,183,971 to 3,184,096
_RS16075
ABIE40_RS16080: ABIE40_RS16080 - hypothetical protein, at 3,184,208 to 3,184,822
_RS16080
ABIE40_RS16085: ABIE40_RS16085 - alpha/beta hydrolase, at 3,184,885 to 3,185,898
_RS16085
Position (kb)
3184
3185 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3183.216 kb on - strand, within ABIE40_RS16070 at 3183.216 kb on - strand, within ABIE40_RS16070 at 3183.387 kb on + strand, within ABIE40_RS16070 at 3183.387 kb on + strand, within ABIE40_RS16070 at 3183.387 kb on + strand, within ABIE40_RS16070 at 3183.388 kb on - strand, within ABIE40_RS16070 at 3183.388 kb on - strand, within ABIE40_RS16070 at 3183.814 kb on + strand at 3183.888 kb on - strand at 3183.920 kb on + strand at 3183.921 kb on - strand at 3184.049 kb on + strand, within ABIE40_RS16075 at 3184.099 kb on + strand at 3184.099 kb on + strand at 3184.099 kb on + strand at 3184.204 kb on + strand at 3184.204 kb on + strand at 3184.205 kb on + strand at 3184.309 kb on + strand, within ABIE40_RS16080 at 3184.309 kb on + strand, within ABIE40_RS16080 at 3184.309 kb on + strand, within ABIE40_RS16080 at 3184.310 kb on - strand, within ABIE40_RS16080 at 3184.310 kb on - strand, within ABIE40_RS16080 at 3184.310 kb on - strand, within ABIE40_RS16080 at 3184.365 kb on - strand, within ABIE40_RS16080 at 3184.365 kb on - strand, within ABIE40_RS16080 at 3184.556 kb on + strand, within ABIE40_RS16080 at 3184.556 kb on + strand, within ABIE40_RS16080 at 3184.556 kb on + strand, within ABIE40_RS16080 at 3184.556 kb on + strand, within ABIE40_RS16080 at 3184.557 kb on - strand, within ABIE40_RS16080 at 3184.557 kb on - strand, within ABIE40_RS16080 at 3184.557 kb on - strand, within ABIE40_RS16080 at 3184.557 kb on - strand, within ABIE40_RS16080 at 3184.592 kb on + strand, within ABIE40_RS16080 at 3184.592 kb on + strand, within ABIE40_RS16080 at 3184.592 kb on + strand, within ABIE40_RS16080 at 3184.592 kb on + strand, within ABIE40_RS16080 at 3184.592 kb on + strand, within ABIE40_RS16080 at 3184.593 kb on - strand, within ABIE40_RS16080 at 3184.593 kb on - strand, within ABIE40_RS16080 at 3184.593 kb on - strand, within ABIE40_RS16080 at 3184.593 kb on - strand, within ABIE40_RS16080 at 3184.593 kb on - strand, within ABIE40_RS16080 at 3184.593 kb on - strand, within ABIE40_RS16080 at 3184.593 kb on - strand, within ABIE40_RS16080 at 3184.607 kb on + strand, within ABIE40_RS16080 at 3184.607 kb on + strand, within ABIE40_RS16080 at 3184.607 kb on + strand, within ABIE40_RS16080 at 3184.852 kb on + strand at 3184.852 kb on + strand at 3184.852 kb on + strand at 3184.853 kb on - strand at 3184.858 kb on + strand at 3184.859 kb on - strand at 3184.865 kb on + strand at 3184.865 kb on + strand at 3184.865 kb on + strand at 3184.865 kb on + strand at 3184.866 kb on - strand at 3184.866 kb on - strand at 3184.866 kb on - strand at 3184.866 kb on - strand at 3184.866 kb on - strand at 3184.866 kb on - strand at 3184.866 kb on - strand at 3184.945 kb on + strand at 3184.945 kb on + strand at 3184.945 kb on + strand at 3184.945 kb on + strand at 3184.945 kb on + strand at 3184.945 kb on + strand at 3184.946 kb on - strand at 3184.946 kb on - strand at 3184.946 kb on - strand at 3184.946 kb on - strand at 3184.946 kb on - strand at 3184.946 kb on - strand at 3185.071 kb on - strand, within ABIE40_RS16085 at 3185.235 kb on - strand, within ABIE40_RS16085 at 3185.235 kb on - strand, within ABIE40_RS16085 at 3185.281 kb on + strand, within ABIE40_RS16085 at 3185.392 kb on + strand, within ABIE40_RS16085 at 3185.392 kb on + strand, within ABIE40_RS16085 at 3185.393 kb on - strand, within ABIE40_RS16085 at 3185.395 kb on + strand, within ABIE40_RS16085 at 3185.395 kb on + strand, within ABIE40_RS16085 at 3185.395 kb on + strand, within ABIE40_RS16085 at 3185.395 kb on + strand, within ABIE40_RS16085 at 3185.395 kb on + strand, within ABIE40_RS16085 at 3185.395 kb on + strand, within ABIE40_RS16085 at 3185.396 kb on - strand, within ABIE40_RS16085 at 3185.396 kb on - strand, within ABIE40_RS16085 at 3185.396 kb on - strand, within ABIE40_RS16085 at 3185.521 kb on + strand, within ABIE40_RS16085 at 3185.521 kb on + strand, within ABIE40_RS16085 at 3185.522 kb on - strand, within ABIE40_RS16085 at 3185.522 kb on - strand, within ABIE40_RS16085 at 3185.522 kb on - strand, within ABIE40_RS16085 at 3185.522 kb on - strand, within ABIE40_RS16085 at 3185.522 kb on - strand, within ABIE40_RS16085 at 3185.523 kb on + strand, within ABIE40_RS16085 at 3185.524 kb on - strand, within ABIE40_RS16085 at 3185.563 kb on - strand, within ABIE40_RS16085 at 3185.569 kb on + strand, within ABIE40_RS16085 at 3185.569 kb on + strand, within ABIE40_RS16085 at 3185.570 kb on - strand, within ABIE40_RS16085 at 3185.570 kb on - strand, within ABIE40_RS16085 at 3185.570 kb on - strand, within ABIE40_RS16085 at 3185.605 kb on + strand, within ABIE40_RS16085 at 3185.606 kb on - strand, within ABIE40_RS16085 at 3185.606 kb on - strand, within ABIE40_RS16085 at 3185.660 kb on + strand, within ABIE40_RS16085 at 3185.660 kb on + strand, within ABIE40_RS16085 at 3185.661 kb on - strand, within ABIE40_RS16085 at 3185.661 kb on - strand, within ABIE40_RS16085 at 3185.661 kb on - strand, within ABIE40_RS16085 at 3185.661 kb on - strand, within ABIE40_RS16085 at 3185.661 kb on - strand, within ABIE40_RS16085 at 3185.773 kb on + strand, within ABIE40_RS16085 at 3185.773 kb on + strand, within ABIE40_RS16085 at 3185.774 kb on - strand, within ABIE40_RS16085 at 3185.774 kb on - strand, within ABIE40_RS16085 at 3185.789 kb on + strand, within ABIE40_RS16085 at 3185.790 kb on - strand, within ABIE40_RS16085 at 3185.790 kb on - strand, within ABIE40_RS16085 at 3185.790 kb on - strand, within ABIE40_RS16085
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with 1-octen-3-ol 1.08545883333333 mM remove 3,183,216 - ABIE40_RS16070 0.44 -1.0 3,183,216 - ABIE40_RS16070 0.44 +2.0 3,183,387 + ABIE40_RS16070 0.60 +1.0 3,183,387 + ABIE40_RS16070 0.60 -1.3 3,183,387 + ABIE40_RS16070 0.60 -0.3 3,183,388 - ABIE40_RS16070 0.60 +2.0 3,183,388 - ABIE40_RS16070 0.60 +0.4 3,183,814 + +0.4 3,183,888 - +1.0 3,183,920 + -1.2 3,183,921 - -0.1 3,184,049 + ABIE40_RS16075 0.62 -1.0 3,184,099 + -0.7 3,184,099 + +0.7 3,184,099 + -0.8 3,184,204 + -0.0 3,184,204 + -1.9 3,184,205 + -0.3 3,184,309 + ABIE40_RS16080 0.16 -2.1 3,184,309 + ABIE40_RS16080 0.16 -0.8 3,184,309 + ABIE40_RS16080 0.16 -0.1 3,184,310 - ABIE40_RS16080 0.17 +0.6 3,184,310 - ABIE40_RS16080 0.17 +0.4 3,184,310 - ABIE40_RS16080 0.17 -0.0 3,184,365 - ABIE40_RS16080 0.26 -1.3 3,184,365 - ABIE40_RS16080 0.26 +1.1 3,184,556 + ABIE40_RS16080 0.57 +0.4 3,184,556 + ABIE40_RS16080 0.57 +0.3 3,184,556 + ABIE40_RS16080 0.57 +1.7 3,184,556 + ABIE40_RS16080 0.57 -2.5 3,184,557 - ABIE40_RS16080 0.57 -1.7 3,184,557 - ABIE40_RS16080 0.57 +0.5 3,184,557 - ABIE40_RS16080 0.57 -0.5 3,184,557 - ABIE40_RS16080 0.57 -2.2 3,184,592 + ABIE40_RS16080 0.62 -1.9 3,184,592 + ABIE40_RS16080 0.62 -1.7 3,184,592 + ABIE40_RS16080 0.62 -0.3 3,184,592 + ABIE40_RS16080 0.62 -1.3 3,184,592 + ABIE40_RS16080 0.62 -2.5 3,184,593 - ABIE40_RS16080 0.63 +0.7 3,184,593 - ABIE40_RS16080 0.63 -1.9 3,184,593 - ABIE40_RS16080 0.63 -0.5 3,184,593 - ABIE40_RS16080 0.63 +0.8 3,184,593 - ABIE40_RS16080 0.63 +0.5 3,184,593 - ABIE40_RS16080 0.63 +0.4 3,184,593 - ABIE40_RS16080 0.63 +0.4 3,184,607 + ABIE40_RS16080 0.65 -0.8 3,184,607 + ABIE40_RS16080 0.65 -2.0 3,184,607 + ABIE40_RS16080 0.65 +0.4 3,184,852 + +0.2 3,184,852 + -2.6 3,184,852 + -1.0 3,184,853 - -0.0 3,184,858 + +0.1 3,184,859 - -0.9 3,184,865 + -0.1 3,184,865 + -1.5 3,184,865 + +0.8 3,184,865 + -2.3 3,184,866 - -1.5 3,184,866 - +0.3 3,184,866 - +0.6 3,184,866 - -0.3 3,184,866 - -1.3 3,184,866 - -1.2 3,184,866 - -0.1 3,184,945 + -2.8 3,184,945 + -0.6 3,184,945 + -0.3 3,184,945 + -0.2 3,184,945 + +0.4 3,184,945 + +0.4 3,184,946 - -0.2 3,184,946 - -2.0 3,184,946 - -0.6 3,184,946 - +0.1 3,184,946 - +2.0 3,184,946 - -1.9 3,185,071 - ABIE40_RS16085 0.18 -1.9 3,185,235 - ABIE40_RS16085 0.35 +1.5 3,185,235 - ABIE40_RS16085 0.35 +0.3 3,185,281 + ABIE40_RS16085 0.39 -0.8 3,185,392 + ABIE40_RS16085 0.50 +1.7 3,185,392 + ABIE40_RS16085 0.50 +0.6 3,185,393 - ABIE40_RS16085 0.50 -0.6 3,185,395 + ABIE40_RS16085 0.50 -1.9 3,185,395 + ABIE40_RS16085 0.50 -0.8 3,185,395 + ABIE40_RS16085 0.50 -2.2 3,185,395 + ABIE40_RS16085 0.50 +0.2 3,185,395 + ABIE40_RS16085 0.50 -0.6 3,185,395 + ABIE40_RS16085 0.50 -2.5 3,185,396 - ABIE40_RS16085 0.50 -0.0 3,185,396 - ABIE40_RS16085 0.50 +0.2 3,185,396 - ABIE40_RS16085 0.50 -0.2 3,185,521 + ABIE40_RS16085 0.63 -1.2 3,185,521 + ABIE40_RS16085 0.63 -1.3 3,185,522 - ABIE40_RS16085 0.63 -1.2 3,185,522 - ABIE40_RS16085 0.63 -0.8 3,185,522 - ABIE40_RS16085 0.63 -1.4 3,185,522 - ABIE40_RS16085 0.63 +0.3 3,185,522 - ABIE40_RS16085 0.63 +0.2 3,185,523 + ABIE40_RS16085 0.63 -1.5 3,185,524 - ABIE40_RS16085 0.63 +1.2 3,185,563 - ABIE40_RS16085 0.67 +0.2 3,185,569 + ABIE40_RS16085 0.67 -0.6 3,185,569 + ABIE40_RS16085 0.67 -1.2 3,185,570 - ABIE40_RS16085 0.68 -1.0 3,185,570 - ABIE40_RS16085 0.68 +1.0 3,185,570 - ABIE40_RS16085 0.68 -0.3 3,185,605 + ABIE40_RS16085 0.71 -1.8 3,185,606 - ABIE40_RS16085 0.71 -0.8 3,185,606 - ABIE40_RS16085 0.71 +0.1 3,185,660 + ABIE40_RS16085 0.76 +0.1 3,185,660 + ABIE40_RS16085 0.76 +0.5 3,185,661 - ABIE40_RS16085 0.77 +0.4 3,185,661 - ABIE40_RS16085 0.77 +0.5 3,185,661 - ABIE40_RS16085 0.77 +0.5 3,185,661 - ABIE40_RS16085 0.77 +0.0 3,185,661 - ABIE40_RS16085 0.77 -0.1 3,185,773 + ABIE40_RS16085 0.88 -0.0 3,185,773 + ABIE40_RS16085 0.88 -0.5 3,185,774 - ABIE40_RS16085 0.88 +1.5 3,185,774 - ABIE40_RS16085 0.88 -0.3 3,185,789 + ABIE40_RS16085 0.89 +0.3 3,185,790 - ABIE40_RS16085 0.89 +0.8 3,185,790 - ABIE40_RS16085 0.89 -2.6 3,185,790 - ABIE40_RS16085 0.89 -0.4
Or see this region's nucleotide sequence