Experiment: R2A with 1-octen-3-ol 1.08545883333333 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE40_RS08965 and ABIE40_RS08970 are separated by 32 nucleotides ABIE40_RS08970 and ABIE40_RS08975 are separated by 399 nucleotides
ABIE40_RS08965: ABIE40_RS08965 - lipid-A-disaccharide synthase, at 1,726,109 to 1,727,284
_RS08965
ABIE40_RS08970: ABIE40_RS08970 - citrate synthase, at 1,727,317 to 1,728,607
_RS08970
ABIE40_RS08975: ABIE40_RS08975 - ComEC/Rec2 family competence protein, at 1,729,007 to 1,731,325
_RS08975
Position (kb)
1727
1728
1729 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1727.339 kb on + strand at 1727.340 kb on - strand at 1727.340 kb on - strand at 1727.363 kb on + strand at 1727.363 kb on + strand at 1727.364 kb on - strand at 1727.364 kb on - strand at 1727.511 kb on + strand, within ABIE40_RS08970 at 1727.511 kb on + strand, within ABIE40_RS08970 at 1727.511 kb on + strand, within ABIE40_RS08970 at 1727.530 kb on - strand, within ABIE40_RS08970 at 1727.673 kb on + strand, within ABIE40_RS08970 at 1727.674 kb on - strand, within ABIE40_RS08970 at 1727.674 kb on - strand, within ABIE40_RS08970 at 1727.745 kb on + strand, within ABIE40_RS08970 at 1727.746 kb on - strand, within ABIE40_RS08970 at 1727.746 kb on - strand, within ABIE40_RS08970 at 1727.941 kb on + strand, within ABIE40_RS08970 at 1727.941 kb on + strand, within ABIE40_RS08970 at 1727.942 kb on - strand, within ABIE40_RS08970 at 1727.942 kb on - strand, within ABIE40_RS08970 at 1727.979 kb on + strand, within ABIE40_RS08970 at 1727.979 kb on + strand, within ABIE40_RS08970 at 1727.980 kb on - strand, within ABIE40_RS08970 at 1728.027 kb on + strand, within ABIE40_RS08970 at 1728.027 kb on + strand, within ABIE40_RS08970 at 1728.027 kb on + strand, within ABIE40_RS08970 at 1728.079 kb on - strand, within ABIE40_RS08970 at 1728.171 kb on + strand, within ABIE40_RS08970 at 1728.171 kb on + strand, within ABIE40_RS08970 at 1728.171 kb on + strand, within ABIE40_RS08970 at 1728.172 kb on - strand, within ABIE40_RS08970 at 1728.334 kb on - strand, within ABIE40_RS08970 at 1728.345 kb on + strand, within ABIE40_RS08970 at 1728.345 kb on + strand, within ABIE40_RS08970 at 1728.346 kb on - strand, within ABIE40_RS08970 at 1728.346 kb on - strand, within ABIE40_RS08970 at 1728.346 kb on - strand, within ABIE40_RS08970 at 1728.432 kb on + strand, within ABIE40_RS08970 at 1728.432 kb on + strand, within ABIE40_RS08970 at 1728.433 kb on - strand, within ABIE40_RS08970 at 1728.433 kb on - strand, within ABIE40_RS08970 at 1728.480 kb on + strand at 1728.480 kb on + strand at 1728.481 kb on - strand at 1728.481 kb on - strand at 1728.481 kb on - strand at 1728.481 kb on - strand at 1728.649 kb on - strand at 1728.649 kb on - strand at 1728.674 kb on + strand at 1728.674 kb on + strand at 1728.675 kb on - strand at 1728.675 kb on - strand at 1728.675 kb on - strand at 1728.675 kb on - strand at 1728.730 kb on - strand at 1728.740 kb on - strand at 1728.767 kb on + strand at 1728.833 kb on - strand at 1728.897 kb on - strand at 1728.983 kb on + strand at 1729.018 kb on - strand at 1729.192 kb on + strand at 1729.192 kb on + strand at 1729.192 kb on + strand at 1729.192 kb on + strand at 1729.193 kb on - strand at 1729.193 kb on - strand at 1729.193 kb on - strand at 1729.193 kb on - strand at 1729.194 kb on + strand at 1729.194 kb on + strand at 1729.194 kb on + strand at 1729.195 kb on - strand at 1729.195 kb on - strand at 1729.422 kb on - strand, within ABIE40_RS08975 at 1729.422 kb on - strand, within ABIE40_RS08975 at 1729.604 kb on + strand, within ABIE40_RS08975 at 1729.604 kb on + strand, within ABIE40_RS08975 at 1729.604 kb on + strand, within ABIE40_RS08975 at 1729.604 kb on + strand, within ABIE40_RS08975 at 1729.605 kb on - strand, within ABIE40_RS08975 at 1729.605 kb on - strand, within ABIE40_RS08975
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with 1-octen-3-ol 1.08545883333333 mM remove 1,727,339 + -3.7 1,727,340 - -0.7 1,727,340 - -0.2 1,727,363 + -0.7 1,727,363 + -3.1 1,727,364 - -0.0 1,727,364 - -0.6 1,727,511 + ABIE40_RS08970 0.15 -0.7 1,727,511 + ABIE40_RS08970 0.15 -1.3 1,727,511 + ABIE40_RS08970 0.15 -0.7 1,727,530 - ABIE40_RS08970 0.16 -2.9 1,727,673 + ABIE40_RS08970 0.28 -0.2 1,727,674 - ABIE40_RS08970 0.28 +0.6 1,727,674 - ABIE40_RS08970 0.28 -3.6 1,727,745 + ABIE40_RS08970 0.33 -1.2 1,727,746 - ABIE40_RS08970 0.33 -1.0 1,727,746 - ABIE40_RS08970 0.33 -0.9 1,727,941 + ABIE40_RS08970 0.48 -3.2 1,727,941 + ABIE40_RS08970 0.48 -0.7 1,727,942 - ABIE40_RS08970 0.48 -1.2 1,727,942 - ABIE40_RS08970 0.48 -0.4 1,727,979 + ABIE40_RS08970 0.51 -1.5 1,727,979 + ABIE40_RS08970 0.51 -1.6 1,727,980 - ABIE40_RS08970 0.51 -0.4 1,728,027 + ABIE40_RS08970 0.55 -3.2 1,728,027 + ABIE40_RS08970 0.55 -0.9 1,728,027 + ABIE40_RS08970 0.55 -0.4 1,728,079 - ABIE40_RS08970 0.59 -2.6 1,728,171 + ABIE40_RS08970 0.66 -2.7 1,728,171 + ABIE40_RS08970 0.66 -1.0 1,728,171 + ABIE40_RS08970 0.66 -1.5 1,728,172 - ABIE40_RS08970 0.66 -0.9 1,728,334 - ABIE40_RS08970 0.79 -0.9 1,728,345 + ABIE40_RS08970 0.80 -1.6 1,728,345 + ABIE40_RS08970 0.80 -2.2 1,728,346 - ABIE40_RS08970 0.80 -1.8 1,728,346 - ABIE40_RS08970 0.80 -0.4 1,728,346 - ABIE40_RS08970 0.80 -3.3 1,728,432 + ABIE40_RS08970 0.86 -2.5 1,728,432 + ABIE40_RS08970 0.86 -2.1 1,728,433 - ABIE40_RS08970 0.86 -1.1 1,728,433 - ABIE40_RS08970 0.86 -1.5 1,728,480 + -2.0 1,728,480 + -3.1 1,728,481 - +0.8 1,728,481 - -2.1 1,728,481 - -1.7 1,728,481 - -1.7 1,728,649 - +0.6 1,728,649 - +0.5 1,728,674 + -1.7 1,728,674 + -1.2 1,728,675 - -0.1 1,728,675 - -1.1 1,728,675 - +1.0 1,728,675 - -0.2 1,728,730 - +2.5 1,728,740 - +1.6 1,728,767 + +0.3 1,728,833 - +0.1 1,728,897 - -1.0 1,728,983 + +0.7 1,729,018 - -0.5 1,729,192 + -2.3 1,729,192 + +0.8 1,729,192 + -0.9 1,729,192 + -1.9 1,729,193 - -0.3 1,729,193 - +0.7 1,729,193 - +1.1 1,729,193 - +0.2 1,729,194 + -1.7 1,729,194 + +0.1 1,729,194 + -1.7 1,729,195 - +0.8 1,729,195 - -0.8 1,729,422 - ABIE40_RS08975 0.18 -2.0 1,729,422 - ABIE40_RS08975 0.18 -0.6 1,729,604 + ABIE40_RS08975 0.26 -0.9 1,729,604 + ABIE40_RS08975 0.26 +0.8 1,729,604 + ABIE40_RS08975 0.26 -0.3 1,729,604 + ABIE40_RS08975 0.26 +1.1 1,729,605 - ABIE40_RS08975 0.26 +0.6 1,729,605 - ABIE40_RS08975 0.26 +1.9
Or see this region's nucleotide sequence