Experiment: R2A with 1-octen-3-ol 1.08545883333333 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE40_RS06465 and ABIE40_RS06470 are separated by 113 nucleotides ABIE40_RS06470 and ABIE40_RS06475 overlap by 4 nucleotides ABIE40_RS06475 and ABIE40_RS06480 are separated by 65 nucleotides ABIE40_RS06480 and ABIE40_RS06485 are separated by 10 nucleotides
ABIE40_RS06465: ABIE40_RS06465 - nucleoside-diphosphate kinase, at 1,267,593 to 1,268,015
_RS06465
ABIE40_RS06470: ABIE40_RS06470 - hypothetical protein, at 1,268,129 to 1,268,638
_RS06470
ABIE40_RS06475: ABIE40_RS06475 - CGNR zinc finger domain-containing protein, at 1,268,635 to 1,269,180
_RS06475
ABIE40_RS06480: ABIE40_RS06480 - branched-chain amino acid ABC transporter permease, at 1,269,246 to 1,270,142
_RS06480
ABIE40_RS06485: ABIE40_RS06485 - molybdenum cofactor biosynthesis protein MoaE, at 1,270,153 to 1,270,608
_RS06485
Position (kb)
1268
1269
1270 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1268.102 kb on + strand at 1268.152 kb on + strand at 1268.153 kb on - strand at 1268.153 kb on - strand at 1268.153 kb on - strand at 1268.153 kb on - strand at 1268.160 kb on + strand at 1268.200 kb on + strand, within ABIE40_RS06470 at 1268.200 kb on + strand, within ABIE40_RS06470 at 1268.201 kb on - strand, within ABIE40_RS06470 at 1268.205 kb on + strand, within ABIE40_RS06470 at 1268.205 kb on + strand, within ABIE40_RS06470 at 1268.206 kb on - strand, within ABIE40_RS06470 at 1268.206 kb on - strand, within ABIE40_RS06470 at 1268.206 kb on - strand, within ABIE40_RS06470 at 1268.206 kb on - strand, within ABIE40_RS06470 at 1268.206 kb on - strand, within ABIE40_RS06470 at 1268.206 kb on - strand, within ABIE40_RS06470 at 1268.206 kb on - strand, within ABIE40_RS06470 at 1268.332 kb on - strand, within ABIE40_RS06470 at 1268.505 kb on + strand, within ABIE40_RS06470 at 1268.506 kb on - strand, within ABIE40_RS06470 at 1268.526 kb on + strand, within ABIE40_RS06470 at 1268.527 kb on - strand, within ABIE40_RS06470 at 1268.734 kb on - strand, within ABIE40_RS06475 at 1268.767 kb on + strand, within ABIE40_RS06475 at 1268.767 kb on + strand, within ABIE40_RS06475 at 1268.768 kb on - strand, within ABIE40_RS06475 at 1268.768 kb on - strand, within ABIE40_RS06475 at 1268.768 kb on - strand, within ABIE40_RS06475 at 1269.172 kb on - strand at 1269.205 kb on + strand at 1269.232 kb on + strand at 1269.232 kb on + strand at 1269.252 kb on + strand at 1269.252 kb on + strand at 1269.253 kb on - strand at 1269.253 kb on - strand at 1269.298 kb on - strand at 1269.298 kb on - strand at 1269.298 kb on - strand at 1269.353 kb on + strand, within ABIE40_RS06480 at 1269.361 kb on - strand, within ABIE40_RS06480 at 1269.361 kb on - strand, within ABIE40_RS06480 at 1269.418 kb on - strand, within ABIE40_RS06480 at 1269.419 kb on + strand, within ABIE40_RS06480 at 1269.419 kb on + strand, within ABIE40_RS06480 at 1269.420 kb on - strand, within ABIE40_RS06480 at 1269.481 kb on - strand, within ABIE40_RS06480 at 1269.481 kb on - strand, within ABIE40_RS06480 at 1269.548 kb on + strand, within ABIE40_RS06480 at 1269.548 kb on + strand, within ABIE40_RS06480 at 1269.548 kb on + strand, within ABIE40_RS06480 at 1269.549 kb on - strand, within ABIE40_RS06480 at 1269.762 kb on + strand, within ABIE40_RS06480 at 1269.763 kb on - strand, within ABIE40_RS06480 at 1269.763 kb on - strand, within ABIE40_RS06480 at 1269.763 kb on - strand, within ABIE40_RS06480 at 1269.764 kb on + strand, within ABIE40_RS06480 at 1269.765 kb on - strand, within ABIE40_RS06480 at 1269.858 kb on + strand, within ABIE40_RS06480 at 1269.858 kb on + strand, within ABIE40_RS06480 at 1269.858 kb on + strand, within ABIE40_RS06480 at 1269.859 kb on - strand, within ABIE40_RS06480 at 1269.908 kb on - strand, within ABIE40_RS06480 at 1269.909 kb on + strand, within ABIE40_RS06480 at 1269.909 kb on + strand, within ABIE40_RS06480 at 1269.909 kb on + strand, within ABIE40_RS06480 at 1269.910 kb on - strand, within ABIE40_RS06480 at 1269.910 kb on - strand, within ABIE40_RS06480 at 1269.910 kb on - strand, within ABIE40_RS06480 at 1269.910 kb on - strand, within ABIE40_RS06480 at 1269.910 kb on - strand, within ABIE40_RS06480 at 1269.912 kb on + strand, within ABIE40_RS06480 at 1269.912 kb on + strand, within ABIE40_RS06480 at 1270.053 kb on + strand at 1270.054 kb on - strand at 1270.054 kb on - strand at 1270.054 kb on - strand at 1270.136 kb on - strand at 1270.136 kb on - strand at 1270.140 kb on + strand at 1270.141 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with 1-octen-3-ol 1.08545883333333 mM remove 1,268,102 + -0.6 1,268,152 + -1.0 1,268,153 - +1.5 1,268,153 - +0.3 1,268,153 - +0.6 1,268,153 - +0.6 1,268,160 + +0.1 1,268,200 + ABIE40_RS06470 0.14 +1.0 1,268,200 + ABIE40_RS06470 0.14 +0.2 1,268,201 - ABIE40_RS06470 0.14 -0.5 1,268,205 + ABIE40_RS06470 0.15 +0.1 1,268,205 + ABIE40_RS06470 0.15 -3.9 1,268,206 - ABIE40_RS06470 0.15 +0.0 1,268,206 - ABIE40_RS06470 0.15 +1.2 1,268,206 - ABIE40_RS06470 0.15 -0.2 1,268,206 - ABIE40_RS06470 0.15 +1.3 1,268,206 - ABIE40_RS06470 0.15 -1.8 1,268,206 - ABIE40_RS06470 0.15 -0.2 1,268,206 - ABIE40_RS06470 0.15 -0.2 1,268,332 - ABIE40_RS06470 0.40 +0.3 1,268,505 + ABIE40_RS06470 0.74 -1.6 1,268,506 - ABIE40_RS06470 0.74 +1.5 1,268,526 + ABIE40_RS06470 0.78 -1.8 1,268,527 - ABIE40_RS06470 0.78 +2.0 1,268,734 - ABIE40_RS06475 0.18 +0.3 1,268,767 + ABIE40_RS06475 0.24 +1.0 1,268,767 + ABIE40_RS06475 0.24 +0.0 1,268,768 - ABIE40_RS06475 0.24 +0.8 1,268,768 - ABIE40_RS06475 0.24 +0.7 1,268,768 - ABIE40_RS06475 0.24 +0.6 1,269,172 - -1.7 1,269,205 + -2.2 1,269,232 + -0.0 1,269,232 + -1.0 1,269,252 + +0.8 1,269,252 + +0.5 1,269,253 - +0.1 1,269,253 - -1.4 1,269,298 - +2.0 1,269,298 - -0.2 1,269,298 - -0.7 1,269,353 + ABIE40_RS06480 0.12 +0.3 1,269,361 - ABIE40_RS06480 0.13 -1.4 1,269,361 - ABIE40_RS06480 0.13 +0.3 1,269,418 - ABIE40_RS06480 0.19 +0.0 1,269,419 + ABIE40_RS06480 0.19 -1.2 1,269,419 + ABIE40_RS06480 0.19 -0.2 1,269,420 - ABIE40_RS06480 0.19 +0.5 1,269,481 - ABIE40_RS06480 0.26 +0.3 1,269,481 - ABIE40_RS06480 0.26 +0.4 1,269,548 + ABIE40_RS06480 0.34 -0.1 1,269,548 + ABIE40_RS06480 0.34 -0.2 1,269,548 + ABIE40_RS06480 0.34 -0.1 1,269,549 - ABIE40_RS06480 0.34 -0.8 1,269,762 + ABIE40_RS06480 0.58 -1.4 1,269,763 - ABIE40_RS06480 0.58 +1.1 1,269,763 - ABIE40_RS06480 0.58 +0.1 1,269,763 - ABIE40_RS06480 0.58 -0.3 1,269,764 + ABIE40_RS06480 0.58 -1.0 1,269,765 - ABIE40_RS06480 0.58 +0.0 1,269,858 + ABIE40_RS06480 0.68 +0.1 1,269,858 + ABIE40_RS06480 0.68 +1.1 1,269,858 + ABIE40_RS06480 0.68 +0.0 1,269,859 - ABIE40_RS06480 0.68 -0.2 1,269,908 - ABIE40_RS06480 0.74 -0.2 1,269,909 + ABIE40_RS06480 0.74 -0.4 1,269,909 + ABIE40_RS06480 0.74 -1.4 1,269,909 + ABIE40_RS06480 0.74 -0.6 1,269,910 - ABIE40_RS06480 0.74 +0.0 1,269,910 - ABIE40_RS06480 0.74 -1.8 1,269,910 - ABIE40_RS06480 0.74 -0.4 1,269,910 - ABIE40_RS06480 0.74 -2.3 1,269,910 - ABIE40_RS06480 0.74 +0.4 1,269,912 + ABIE40_RS06480 0.74 -0.2 1,269,912 + ABIE40_RS06480 0.74 +0.6 1,270,053 + -0.1 1,270,054 - +0.7 1,270,054 - -0.7 1,270,054 - -1.1 1,270,136 - +0.2 1,270,136 - +2.0 1,270,140 + +1.2 1,270,141 - +0.6
Or see this region's nucleotide sequence