Experiment: R2A with 1-octen-3-ol 1.08545883333333 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE40_RS27135 and ABIE40_RS27140 are separated by 69 nucleotides ABIE40_RS27140 and ABIE40_RS27145 are separated by 108 nucleotides ABIE40_RS27145 and ABIE40_RS27150 are separated by 61 nucleotides
ABIE40_RS27135: ABIE40_RS27135 - CHAD domain-containing protein, at 145,724 to 147,178
_RS27135
ABIE40_RS27140: ABIE40_RS27140 - DUF2934 domain-containing protein, at 147,248 to 147,652
_RS27140
ABIE40_RS27145: ABIE40_RS27145 - hypothetical protein, at 147,761 to 148,021
_RS27145
ABIE40_RS27150: ABIE40_RS27150 - NUDIX hydrolase, at 148,083 to 148,550
_RS27150
Position (kb) 147
148
149 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 146.909 kb on + strand, within ABIE40_RS27135 at 146.910 kb on - strand, within ABIE40_RS27135 at 146.965 kb on + strand, within ABIE40_RS27135 at 146.965 kb on + strand, within ABIE40_RS27135 at 146.966 kb on - strand, within ABIE40_RS27135 at 147.092 kb on + strand at 147.092 kb on + strand at 147.092 kb on + strand at 147.093 kb on - strand at 147.177 kb on - strand at 147.203 kb on + strand at 147.204 kb on - strand at 147.204 kb on - strand at 147.338 kb on - strand, within ABIE40_RS27140 at 147.440 kb on - strand, within ABIE40_RS27140 at 147.442 kb on - strand, within ABIE40_RS27140 at 147.505 kb on + strand, within ABIE40_RS27140 at 147.505 kb on + strand, within ABIE40_RS27140 at 147.505 kb on + strand, within ABIE40_RS27140 at 147.505 kb on + strand, within ABIE40_RS27140 at 147.505 kb on + strand, within ABIE40_RS27140 at 147.506 kb on - strand, within ABIE40_RS27140 at 147.506 kb on - strand, within ABIE40_RS27140 at 147.506 kb on - strand, within ABIE40_RS27140 at 147.506 kb on - strand, within ABIE40_RS27140 at 147.506 kb on - strand, within ABIE40_RS27140 at 147.593 kb on - strand, within ABIE40_RS27140 at 147.593 kb on - strand, within ABIE40_RS27140 at 147.602 kb on + strand, within ABIE40_RS27140 at 147.706 kb on + strand at 147.707 kb on - strand at 147.707 kb on - strand at 147.734 kb on - strand at 147.790 kb on + strand, within ABIE40_RS27145 at 147.790 kb on + strand, within ABIE40_RS27145 at 147.790 kb on + strand, within ABIE40_RS27145 at 147.790 kb on + strand, within ABIE40_RS27145 at 147.791 kb on - strand, within ABIE40_RS27145 at 147.791 kb on - strand, within ABIE40_RS27145 at 147.911 kb on + strand, within ABIE40_RS27145 at 147.911 kb on + strand, within ABIE40_RS27145 at 147.912 kb on - strand, within ABIE40_RS27145 at 147.960 kb on + strand, within ABIE40_RS27145 at 147.986 kb on - strand, within ABIE40_RS27145 at 148.093 kb on + strand at 148.094 kb on - strand at 148.094 kb on - strand at 148.097 kb on + strand at 148.097 kb on + strand at 148.097 kb on + strand at 148.097 kb on + strand at 148.098 kb on - strand at 148.098 kb on - strand at 148.098 kb on - strand at 148.141 kb on + strand, within ABIE40_RS27150 at 148.141 kb on + strand, within ABIE40_RS27150 at 148.141 kb on + strand, within ABIE40_RS27150 at 148.141 kb on + strand, within ABIE40_RS27150 at 148.142 kb on - strand, within ABIE40_RS27150 at 148.185 kb on - strand, within ABIE40_RS27150 at 148.329 kb on + strand, within ABIE40_RS27150 at 148.353 kb on - strand, within ABIE40_RS27150 at 148.380 kb on + strand, within ABIE40_RS27150 at 148.380 kb on + strand, within ABIE40_RS27150 at 148.380 kb on + strand, within ABIE40_RS27150 at 148.381 kb on - strand, within ABIE40_RS27150 at 148.381 kb on - strand, within ABIE40_RS27150 at 148.386 kb on - strand, within ABIE40_RS27150 at 148.441 kb on + strand, within ABIE40_RS27150 at 148.441 kb on + strand, within ABIE40_RS27150 at 148.442 kb on - strand, within ABIE40_RS27150 at 148.481 kb on - strand, within ABIE40_RS27150 at 148.556 kb on + strand at 148.556 kb on + strand at 148.557 kb on - strand at 148.561 kb on - strand at 148.561 kb on - strand at 148.678 kb on + strand at 148.678 kb on + strand at 148.752 kb on + strand at 148.752 kb on + strand at 148.923 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with 1-octen-3-ol 1.08545883333333 mM remove 146,909 + ABIE40_RS27135 0.81 -1.1 146,910 - ABIE40_RS27135 0.82 -1.2 146,965 + ABIE40_RS27135 0.85 -1.0 146,965 + ABIE40_RS27135 0.85 -0.4 146,966 - ABIE40_RS27135 0.85 -1.3 147,092 + -0.9 147,092 + -0.0 147,092 + +3.5 147,093 - -2.7 147,177 - -1.2 147,203 + -2.5 147,204 - +0.3 147,204 - -1.5 147,338 - ABIE40_RS27140 0.22 +2.6 147,440 - ABIE40_RS27140 0.47 -1.3 147,442 - ABIE40_RS27140 0.48 +1.0 147,505 + ABIE40_RS27140 0.63 -1.7 147,505 + ABIE40_RS27140 0.63 +2.0 147,505 + ABIE40_RS27140 0.63 -1.9 147,505 + ABIE40_RS27140 0.63 +1.3 147,505 + ABIE40_RS27140 0.63 -2.7 147,506 - ABIE40_RS27140 0.64 +1.6 147,506 - ABIE40_RS27140 0.64 -0.9 147,506 - ABIE40_RS27140 0.64 +0.3 147,506 - ABIE40_RS27140 0.64 +1.5 147,506 - ABIE40_RS27140 0.64 -1.8 147,593 - ABIE40_RS27140 0.85 -2.7 147,593 - ABIE40_RS27140 0.85 -0.7 147,602 + ABIE40_RS27140 0.87 -1.7 147,706 + +0.7 147,707 - -0.0 147,707 - -0.8 147,734 - +1.0 147,790 + ABIE40_RS27145 0.11 -2.0 147,790 + ABIE40_RS27145 0.11 +0.2 147,790 + ABIE40_RS27145 0.11 -0.9 147,790 + ABIE40_RS27145 0.11 +0.6 147,791 - ABIE40_RS27145 0.11 -1.2 147,791 - ABIE40_RS27145 0.11 +2.7 147,911 + ABIE40_RS27145 0.57 -1.2 147,911 + ABIE40_RS27145 0.57 -2.0 147,912 - ABIE40_RS27145 0.58 +1.0 147,960 + ABIE40_RS27145 0.76 -1.0 147,986 - ABIE40_RS27145 0.86 -2.6 148,093 + +1.1 148,094 - +2.6 148,094 - -1.0 148,097 + +1.4 148,097 + -0.2 148,097 + -0.2 148,097 + -3.1 148,098 - +2.1 148,098 - +0.2 148,098 - +1.6 148,141 + ABIE40_RS27150 0.12 +1.1 148,141 + ABIE40_RS27150 0.12 -1.3 148,141 + ABIE40_RS27150 0.12 -0.6 148,141 + ABIE40_RS27150 0.12 +1.4 148,142 - ABIE40_RS27150 0.13 -0.0 148,185 - ABIE40_RS27150 0.22 -0.9 148,329 + ABIE40_RS27150 0.53 +1.0 148,353 - ABIE40_RS27150 0.58 +2.6 148,380 + ABIE40_RS27150 0.63 +1.1 148,380 + ABIE40_RS27150 0.63 +1.0 148,380 + ABIE40_RS27150 0.63 +1.0 148,381 - ABIE40_RS27150 0.64 -1.7 148,381 - ABIE40_RS27150 0.64 +1.2 148,386 - ABIE40_RS27150 0.65 -1.1 148,441 + ABIE40_RS27150 0.76 +0.3 148,441 + ABIE40_RS27150 0.76 +2.6 148,442 - ABIE40_RS27150 0.77 -2.2 148,481 - ABIE40_RS27150 0.85 +2.6 148,556 + +2.0 148,556 + +3.6 148,557 - -2.8 148,561 - -0.6 148,561 - +0.3 148,678 + +2.9 148,678 + -0.2 148,752 + +0.3 148,752 + -2.9 148,923 + +2.6
Or see this region's nucleotide sequence