Experiment: R2A with 1-octen-3-ol 1.08545883333333 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE40_RS22905 and ABIE40_RS22910 are separated by 289 nucleotides ABIE40_RS22910 and ABIE40_RS22915 are separated by 390 nucleotides ABIE40_RS22915 and ABIE40_RS22920 are separated by 21 nucleotides
ABIE40_RS22905: ABIE40_RS22905 - 5S ribosomal RNA, at 728,247 to 728,361
_RS22905
ABIE40_RS22910: ABIE40_RS22910 - tRNA-Met, at 728,651 to 728,727
_RS22910
ABIE40_RS22915: ABIE40_RS22915 - MsnO8 family LLM class oxidoreductase, at 729,118 to 730,110
_RS22915
ABIE40_RS22920: ABIE40_RS22920 - LLM class flavin-dependent oxidoreductase, at 730,132 to 731,481
_RS22920
Position (kb)
729
730
731 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 728.588 kb on + strand at 728.594 kb on + strand at 728.594 kb on + strand at 728.614 kb on - strand at 728.783 kb on - strand at 728.786 kb on + strand at 728.786 kb on + strand at 728.786 kb on + strand at 728.787 kb on - strand at 728.787 kb on - strand at 728.787 kb on - strand at 728.990 kb on - strand at 728.990 kb on - strand at 729.124 kb on + strand at 729.124 kb on + strand at 729.124 kb on + strand at 729.124 kb on + strand at 729.124 kb on + strand at 729.125 kb on - strand at 729.125 kb on - strand at 729.125 kb on - strand at 729.125 kb on - strand at 729.125 kb on - strand at 729.229 kb on + strand, within ABIE40_RS22915 at 729.258 kb on + strand, within ABIE40_RS22915 at 729.258 kb on + strand, within ABIE40_RS22915 at 729.258 kb on + strand, within ABIE40_RS22915 at 729.259 kb on - strand, within ABIE40_RS22915 at 729.259 kb on - strand, within ABIE40_RS22915 at 729.259 kb on - strand, within ABIE40_RS22915 at 729.259 kb on - strand, within ABIE40_RS22915 at 729.259 kb on - strand, within ABIE40_RS22915 at 729.267 kb on + strand, within ABIE40_RS22915 at 729.304 kb on + strand, within ABIE40_RS22915 at 729.304 kb on + strand, within ABIE40_RS22915 at 729.304 kb on + strand, within ABIE40_RS22915 at 729.305 kb on - strand, within ABIE40_RS22915 at 729.351 kb on - strand, within ABIE40_RS22915 at 729.351 kb on - strand, within ABIE40_RS22915 at 729.351 kb on - strand, within ABIE40_RS22915 at 729.351 kb on - strand, within ABIE40_RS22915 at 729.351 kb on - strand, within ABIE40_RS22915 at 729.351 kb on - strand, within ABIE40_RS22915 at 729.364 kb on + strand, within ABIE40_RS22915 at 729.364 kb on + strand, within ABIE40_RS22915 at 729.364 kb on + strand, within ABIE40_RS22915 at 729.364 kb on + strand, within ABIE40_RS22915 at 729.364 kb on + strand, within ABIE40_RS22915 at 729.364 kb on + strand, within ABIE40_RS22915 at 729.365 kb on - strand, within ABIE40_RS22915 at 729.365 kb on - strand, within ABIE40_RS22915 at 729.365 kb on - strand, within ABIE40_RS22915 at 729.365 kb on - strand, within ABIE40_RS22915 at 729.365 kb on - strand, within ABIE40_RS22915 at 729.365 kb on - strand, within ABIE40_RS22915 at 729.366 kb on + strand, within ABIE40_RS22915 at 729.367 kb on - strand, within ABIE40_RS22915 at 729.367 kb on - strand, within ABIE40_RS22915 at 729.367 kb on - strand, within ABIE40_RS22915 at 729.367 kb on - strand, within ABIE40_RS22915 at 729.373 kb on + strand, within ABIE40_RS22915 at 729.373 kb on + strand, within ABIE40_RS22915 at 729.373 kb on + strand, within ABIE40_RS22915 at 729.374 kb on - strand, within ABIE40_RS22915 at 729.544 kb on + strand, within ABIE40_RS22915 at 729.545 kb on - strand, within ABIE40_RS22915 at 729.589 kb on - strand, within ABIE40_RS22915 at 729.922 kb on + strand, within ABIE40_RS22915 at 729.922 kb on + strand, within ABIE40_RS22915 at 729.923 kb on - strand, within ABIE40_RS22915 at 730.108 kb on + strand at 730.109 kb on - strand at 730.109 kb on - strand at 730.109 kb on - strand at 730.243 kb on + strand at 730.244 kb on - strand at 730.244 kb on - strand at 730.244 kb on - strand at 730.244 kb on - strand at 730.251 kb on + strand at 730.309 kb on + strand, within ABIE40_RS22920 at 730.309 kb on + strand, within ABIE40_RS22920 at 730.309 kb on + strand, within ABIE40_RS22920 at 730.314 kb on + strand, within ABIE40_RS22920 at 730.426 kb on + strand, within ABIE40_RS22920 at 730.426 kb on + strand, within ABIE40_RS22920 at 730.426 kb on + strand, within ABIE40_RS22920 at 730.426 kb on + strand, within ABIE40_RS22920 at 730.427 kb on - strand, within ABIE40_RS22920 at 730.427 kb on - strand, within ABIE40_RS22920 at 730.427 kb on - strand, within ABIE40_RS22920 at 730.537 kb on + strand, within ABIE40_RS22920 at 730.537 kb on + strand, within ABIE40_RS22920 at 730.538 kb on - strand, within ABIE40_RS22920 at 730.538 kb on - strand, within ABIE40_RS22920 at 730.538 kb on - strand, within ABIE40_RS22920 at 730.570 kb on + strand, within ABIE40_RS22920 at 730.588 kb on + strand, within ABIE40_RS22920 at 730.588 kb on + strand, within ABIE40_RS22920 at 730.588 kb on + strand, within ABIE40_RS22920 at 730.588 kb on + strand, within ABIE40_RS22920 at 730.588 kb on + strand, within ABIE40_RS22920 at 730.589 kb on - strand, within ABIE40_RS22920 at 730.589 kb on - strand, within ABIE40_RS22920 at 730.590 kb on + strand, within ABIE40_RS22920 at 730.590 kb on + strand, within ABIE40_RS22920 at 730.590 kb on + strand, within ABIE40_RS22920 at 730.591 kb on - strand, within ABIE40_RS22920 at 730.591 kb on - strand, within ABIE40_RS22920 at 730.663 kb on + strand, within ABIE40_RS22920 at 730.663 kb on + strand, within ABIE40_RS22920 at 730.664 kb on - strand, within ABIE40_RS22920 at 730.664 kb on - strand, within ABIE40_RS22920 at 730.867 kb on + strand, within ABIE40_RS22920 at 730.867 kb on + strand, within ABIE40_RS22920 at 730.867 kb on + strand, within ABIE40_RS22920 at 730.867 kb on + strand, within ABIE40_RS22920 at 730.867 kb on + strand, within ABIE40_RS22920 at 730.868 kb on - strand, within ABIE40_RS22920 at 730.868 kb on - strand, within ABIE40_RS22920 at 730.868 kb on - strand, within ABIE40_RS22920 at 730.927 kb on + strand, within ABIE40_RS22920 at 730.927 kb on + strand, within ABIE40_RS22920 at 730.981 kb on + strand, within ABIE40_RS22920 at 730.981 kb on + strand, within ABIE40_RS22920 at 730.982 kb on - strand, within ABIE40_RS22920 at 730.982 kb on - strand, within ABIE40_RS22920 at 731.005 kb on - strand, within ABIE40_RS22920 at 731.027 kb on - strand, within ABIE40_RS22920 at 731.066 kb on - strand, within ABIE40_RS22920 at 731.066 kb on - strand, within ABIE40_RS22920 at 731.072 kb on + strand, within ABIE40_RS22920
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with 1-octen-3-ol 1.08545883333333 mM remove 728,588 + +2.0 728,594 + -2.1 728,594 + -2.2 728,614 - -2.1 728,783 - +1.2 728,786 + -0.1 728,786 + +0.6 728,786 + -0.6 728,787 - -2.2 728,787 - -0.7 728,787 - +0.1 728,990 - +1.2 728,990 - +1.8 729,124 + +1.4 729,124 + -3.2 729,124 + -1.4 729,124 + -4.0 729,124 + -1.5 729,125 - +2.1 729,125 - -1.6 729,125 - -0.4 729,125 - -0.4 729,125 - -0.4 729,229 + ABIE40_RS22915 0.11 -0.1 729,258 + ABIE40_RS22915 0.14 -0.8 729,258 + ABIE40_RS22915 0.14 +1.5 729,258 + ABIE40_RS22915 0.14 +0.1 729,259 - ABIE40_RS22915 0.14 +2.2 729,259 - ABIE40_RS22915 0.14 -1.4 729,259 - ABIE40_RS22915 0.14 -2.8 729,259 - ABIE40_RS22915 0.14 -1.4 729,259 - ABIE40_RS22915 0.14 -2.0 729,267 + ABIE40_RS22915 0.15 -1.0 729,304 + ABIE40_RS22915 0.19 -2.6 729,304 + ABIE40_RS22915 0.19 +1.6 729,304 + ABIE40_RS22915 0.19 -0.8 729,305 - ABIE40_RS22915 0.19 +1.3 729,351 - ABIE40_RS22915 0.23 -0.5 729,351 - ABIE40_RS22915 0.23 -1.2 729,351 - ABIE40_RS22915 0.23 +1.7 729,351 - ABIE40_RS22915 0.23 -2.4 729,351 - ABIE40_RS22915 0.23 -0.1 729,351 - ABIE40_RS22915 0.23 -0.8 729,364 + ABIE40_RS22915 0.25 -1.8 729,364 + ABIE40_RS22915 0.25 +1.5 729,364 + ABIE40_RS22915 0.25 -0.4 729,364 + ABIE40_RS22915 0.25 +0.9 729,364 + ABIE40_RS22915 0.25 +1.0 729,364 + ABIE40_RS22915 0.25 -1.5 729,365 - ABIE40_RS22915 0.25 -2.9 729,365 - ABIE40_RS22915 0.25 +1.8 729,365 - ABIE40_RS22915 0.25 +0.2 729,365 - ABIE40_RS22915 0.25 +0.2 729,365 - ABIE40_RS22915 0.25 -3.1 729,365 - ABIE40_RS22915 0.25 +1.0 729,366 + ABIE40_RS22915 0.25 -0.1 729,367 - ABIE40_RS22915 0.25 -2.9 729,367 - ABIE40_RS22915 0.25 +0.5 729,367 - ABIE40_RS22915 0.25 -0.4 729,367 - ABIE40_RS22915 0.25 +2.1 729,373 + ABIE40_RS22915 0.26 -0.4 729,373 + ABIE40_RS22915 0.26 +0.8 729,373 + ABIE40_RS22915 0.26 -0.4 729,374 - ABIE40_RS22915 0.26 -1.7 729,544 + ABIE40_RS22915 0.43 +1.7 729,545 - ABIE40_RS22915 0.43 +1.5 729,589 - ABIE40_RS22915 0.47 -1.5 729,922 + ABIE40_RS22915 0.81 +0.8 729,922 + ABIE40_RS22915 0.81 -2.4 729,923 - ABIE40_RS22915 0.81 +1.1 730,108 + -0.1 730,109 - -0.3 730,109 - +0.4 730,109 - -1.0 730,243 + -1.2 730,244 - -1.1 730,244 - -0.1 730,244 - +0.4 730,244 - -0.6 730,251 + +1.3 730,309 + ABIE40_RS22920 0.13 -3.0 730,309 + ABIE40_RS22920 0.13 -0.4 730,309 + ABIE40_RS22920 0.13 +0.3 730,314 + ABIE40_RS22920 0.13 -3.8 730,426 + ABIE40_RS22920 0.22 +0.2 730,426 + ABIE40_RS22920 0.22 +2.1 730,426 + ABIE40_RS22920 0.22 -0.5 730,426 + ABIE40_RS22920 0.22 +1.1 730,427 - ABIE40_RS22920 0.22 -1.0 730,427 - ABIE40_RS22920 0.22 -0.6 730,427 - ABIE40_RS22920 0.22 -1.4 730,537 + ABIE40_RS22920 0.30 -0.0 730,537 + ABIE40_RS22920 0.30 -1.7 730,538 - ABIE40_RS22920 0.30 +1.4 730,538 - ABIE40_RS22920 0.30 -3.6 730,538 - ABIE40_RS22920 0.30 +1.2 730,570 + ABIE40_RS22920 0.32 -2.2 730,588 + ABIE40_RS22920 0.34 +1.2 730,588 + ABIE40_RS22920 0.34 +0.3 730,588 + ABIE40_RS22920 0.34 -0.3 730,588 + ABIE40_RS22920 0.34 -2.7 730,588 + ABIE40_RS22920 0.34 -1.5 730,589 - ABIE40_RS22920 0.34 -0.5 730,589 - ABIE40_RS22920 0.34 +0.9 730,590 + ABIE40_RS22920 0.34 +1.2 730,590 + ABIE40_RS22920 0.34 -2.0 730,590 + ABIE40_RS22920 0.34 +1.4 730,591 - ABIE40_RS22920 0.34 -1.1 730,591 - ABIE40_RS22920 0.34 -2.2 730,663 + ABIE40_RS22920 0.39 -0.2 730,663 + ABIE40_RS22920 0.39 +1.5 730,664 - ABIE40_RS22920 0.39 -3.0 730,664 - ABIE40_RS22920 0.39 -2.1 730,867 + ABIE40_RS22920 0.54 +1.2 730,867 + ABIE40_RS22920 0.54 +0.2 730,867 + ABIE40_RS22920 0.54 +0.5 730,867 + ABIE40_RS22920 0.54 -0.4 730,867 + ABIE40_RS22920 0.54 -3.4 730,868 - ABIE40_RS22920 0.55 -3.1 730,868 - ABIE40_RS22920 0.55 -0.6 730,868 - ABIE40_RS22920 0.55 -1.7 730,927 + ABIE40_RS22920 0.59 -0.8 730,927 + ABIE40_RS22920 0.59 -1.5 730,981 + ABIE40_RS22920 0.63 -2.5 730,981 + ABIE40_RS22920 0.63 -0.2 730,982 - ABIE40_RS22920 0.63 +2.2 730,982 - ABIE40_RS22920 0.63 -2.4 731,005 - ABIE40_RS22920 0.65 -2.0 731,027 - ABIE40_RS22920 0.66 +1.0 731,066 - ABIE40_RS22920 0.69 -3.1 731,066 - ABIE40_RS22920 0.69 -0.6 731,072 + ABIE40_RS22920 0.70 -2.5
Or see this region's nucleotide sequence