Experiment: R2A with 1-octen-3-ol 1.08545883333333 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE40_RS08715 and ABIE40_RS08720 are separated by 974 nucleotides ABIE40_RS08720 and ABIE40_RS08725 are separated by 82 nucleotides ABIE40_RS08725 and ABIE40_RS08730 are separated by 196 nucleotides
ABIE40_RS08715: ABIE40_RS08715 - IS21-like element helper ATPase IstB, at 1,684,399 to 1,685,211
_RS08715
ABIE40_RS08720: ABIE40_RS08720 - tRNA-Glu, at 1,686,186 to 1,686,248
_RS08720
ABIE40_RS08725: ABIE40_RS08725 - tRNA-Glu, at 1,686,331 to 1,686,405
_RS08725
ABIE40_RS08730: ABIE40_RS08730 - D-Ala-D-Ala carboxypeptidase family metallohydrolase, at 1,686,602 to 1,687,693
_RS08730
Position (kb)
1686
1687 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 1685.196 kb on - strand at 1685.209 kb on + strand at 1685.209 kb on + strand at 1685.209 kb on + strand at 1685.209 kb on + strand at 1685.209 kb on + strand at 1685.209 kb on + strand at 1685.209 kb on + strand at 1685.209 kb on + strand at 1685.210 kb on - strand at 1685.210 kb on - strand at 1685.210 kb on - strand at 1685.270 kb on + strand at 1685.270 kb on + strand at 1685.270 kb on + strand at 1685.270 kb on + strand at 1685.271 kb on - strand at 1685.271 kb on - strand at 1685.271 kb on - strand at 1685.271 kb on - strand at 1685.314 kb on + strand at 1685.315 kb on - strand at 1685.315 kb on - strand at 1685.354 kb on + strand at 1685.366 kb on + strand at 1685.366 kb on + strand at 1685.367 kb on - strand at 1685.395 kb on + strand at 1685.395 kb on + strand at 1685.395 kb on + strand at 1685.395 kb on + strand at 1685.396 kb on - strand at 1685.396 kb on - strand at 1685.548 kb on + strand at 1685.548 kb on + strand at 1685.548 kb on + strand at 1685.548 kb on + strand at 1685.549 kb on - strand at 1685.564 kb on - strand at 1685.588 kb on + strand at 1685.588 kb on + strand at 1685.589 kb on - strand at 1685.589 kb on - strand at 1685.589 kb on - strand at 1685.589 kb on - strand at 1685.623 kb on + strand at 1685.624 kb on - strand at 1685.624 kb on - strand at 1685.624 kb on - strand at 1685.624 kb on - strand at 1685.624 kb on - strand at 1685.624 kb on - strand at 1685.643 kb on + strand at 1685.643 kb on + strand at 1685.647 kb on - strand at 1685.677 kb on - strand at 1685.893 kb on + strand at 1685.999 kb on + strand at 1685.999 kb on + strand at 1686.000 kb on - strand at 1686.000 kb on - strand at 1686.000 kb on - strand at 1686.000 kb on - strand at 1686.049 kb on + strand at 1686.049 kb on + strand at 1686.049 kb on + strand at 1686.049 kb on + strand at 1686.050 kb on - strand at 1686.050 kb on - strand at 1686.111 kb on + strand at 1686.118 kb on + strand at 1686.118 kb on + strand at 1686.118 kb on + strand at 1686.119 kb on - strand at 1686.121 kb on + strand at 1686.122 kb on - strand at 1686.122 kb on - strand at 1686.173 kb on + strand at 1686.243 kb on + strand at 1686.244 kb on - strand at 1686.271 kb on - strand at 1686.271 kb on - strand at 1686.294 kb on + strand at 1686.295 kb on - strand at 1686.428 kb on + strand at 1686.428 kb on + strand at 1686.428 kb on + strand at 1686.525 kb on + strand at 1686.697 kb on + strand at 1686.705 kb on + strand at 1686.705 kb on + strand at 1686.705 kb on + strand at 1686.705 kb on + strand at 1686.705 kb on + strand at 1686.705 kb on + strand at 1686.705 kb on + strand at 1686.705 kb on + strand at 1686.706 kb on - strand at 1686.706 kb on - strand at 1686.706 kb on - strand at 1686.744 kb on + strand, within ABIE40_RS08730 at 1686.744 kb on + strand, within ABIE40_RS08730 at 1686.744 kb on + strand, within ABIE40_RS08730 at 1686.745 kb on - strand, within ABIE40_RS08730 at 1686.745 kb on - strand, within ABIE40_RS08730 at 1686.842 kb on - strand, within ABIE40_RS08730 at 1687.062 kb on + strand, within ABIE40_RS08730 at 1687.062 kb on + strand, within ABIE40_RS08730 at 1687.063 kb on - strand, within ABIE40_RS08730 at 1687.063 kb on - strand, within ABIE40_RS08730 at 1687.119 kb on + strand, within ABIE40_RS08730 at 1687.119 kb on + strand, within ABIE40_RS08730 at 1687.145 kb on - strand, within ABIE40_RS08730 at 1687.145 kb on - strand, within ABIE40_RS08730
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with 1-octen-3-ol 1.08545883333333 mM remove 1,685,196 - -1.5 1,685,209 + -0.6 1,685,209 + -0.1 1,685,209 + -0.4 1,685,209 + -3.3 1,685,209 + -0.6 1,685,209 + -0.8 1,685,209 + +1.8 1,685,209 + +2.4 1,685,210 - +2.1 1,685,210 - +1.4 1,685,210 - -4.0 1,685,270 + -0.9 1,685,270 + +0.9 1,685,270 + -1.5 1,685,270 + +1.9 1,685,271 - +0.6 1,685,271 - +2.1 1,685,271 - +2.4 1,685,271 - -2.5 1,685,314 + +1.4 1,685,315 - +0.7 1,685,315 - -1.4 1,685,354 + -1.4 1,685,366 + -2.6 1,685,366 + +1.9 1,685,367 - -0.8 1,685,395 + +0.1 1,685,395 + +0.8 1,685,395 + +0.2 1,685,395 + -0.2 1,685,396 - +0.3 1,685,396 - +1.7 1,685,548 + -2.8 1,685,548 + +0.1 1,685,548 + +1.5 1,685,548 + +2.1 1,685,549 - +1.4 1,685,564 - -0.5 1,685,588 + -0.9 1,685,588 + -0.9 1,685,589 - +0.3 1,685,589 - +0.4 1,685,589 - -0.6 1,685,589 - +0.5 1,685,623 + +2.4 1,685,624 - +2.3 1,685,624 - +2.4 1,685,624 - -2.3 1,685,624 - -1.9 1,685,624 - +2.4 1,685,624 - -1.9 1,685,643 + +0.4 1,685,643 + -1.4 1,685,647 - +0.8 1,685,677 - -1.2 1,685,893 + +2.4 1,685,999 + -2.4 1,685,999 + +0.5 1,686,000 - +2.0 1,686,000 - -2.1 1,686,000 - -1.8 1,686,000 - -0.4 1,686,049 + -1.7 1,686,049 + +0.5 1,686,049 + -0.6 1,686,049 + -2.3 1,686,050 - +0.3 1,686,050 - +0.8 1,686,111 + -2.2 1,686,118 + -2.3 1,686,118 + +0.7 1,686,118 + -0.7 1,686,119 - -2.4 1,686,121 + +0.4 1,686,122 - -0.8 1,686,122 - -2.1 1,686,173 + +1.1 1,686,243 + -0.6 1,686,244 - -3.5 1,686,271 - +0.0 1,686,271 - -1.9 1,686,294 + +0.4 1,686,295 - -1.2 1,686,428 + -1.3 1,686,428 + +3.8 1,686,428 + +0.8 1,686,525 + +0.9 1,686,697 + -1.1 1,686,705 + +0.3 1,686,705 + +0.4 1,686,705 + +0.7 1,686,705 + +0.7 1,686,705 + -1.7 1,686,705 + -2.4 1,686,705 + -1.6 1,686,705 + -0.7 1,686,706 - -2.0 1,686,706 - +0.7 1,686,706 - +0.2 1,686,744 + ABIE40_RS08730 0.13 -2.6 1,686,744 + ABIE40_RS08730 0.13 -0.3 1,686,744 + ABIE40_RS08730 0.13 +0.3 1,686,745 - ABIE40_RS08730 0.13 -2.5 1,686,745 - ABIE40_RS08730 0.13 +0.2 1,686,842 - ABIE40_RS08730 0.22 +1.9 1,687,062 + ABIE40_RS08730 0.42 +2.2 1,687,062 + ABIE40_RS08730 0.42 +1.6 1,687,063 - ABIE40_RS08730 0.42 +0.5 1,687,063 - ABIE40_RS08730 0.42 +1.3 1,687,119 + ABIE40_RS08730 0.47 +1.1 1,687,119 + ABIE40_RS08730 0.47 +0.7 1,687,145 - ABIE40_RS08730 0.50 +1.1 1,687,145 - ABIE40_RS08730 0.50 -1.8
Or see this region's nucleotide sequence