Experiment: R2A with 1-octen-3-ol 1.08545883333333 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE40_RS01825 and ABIE40_RS01830 overlap by 9 nucleotides ABIE40_RS01830 and ABIE40_RS01835 are separated by 112 nucleotides ABIE40_RS01835 and ABIE40_RS01840 are separated by 29 nucleotides ABIE40_RS01840 and ABIE40_RS01845 are separated by 217 nucleotides ABIE40_RS01845 and ABIE40_RS01850 are separated by 15 nucleotides
ABIE40_RS01825: ABIE40_RS01825 - GNAT family N-acetyltransferase, at 361,761 to 362,207
_RS01825
ABIE40_RS01830: ABIE40_RS01830 - LapA family protein, at 362,199 to 362,531
_RS01830
ABIE40_RS01835: ABIE40_RS01835 - integration host factor subunit beta, at 362,644 to 362,943
_RS01835
ABIE40_RS01840: ABIE40_RS01840 - signal peptide peptidase SppA, at 362,973 to 363,923
_RS01840
ABIE40_RS01845: ABIE40_RS01845 - LPS export ABC transporter periplasmic protein LptC, at 364,141 to 364,806
_RS01845
ABIE40_RS01850: ABIE40_RS01850 - LptA/OstA family protein, at 364,822 to 365,436
_RS01850
Position (kb)
362
363
364 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3 at 361.989 kb on + strand, within ABIE40_RS01825 at 361.989 kb on + strand, within ABIE40_RS01825 at 361.989 kb on + strand, within ABIE40_RS01825 at 361.989 kb on + strand, within ABIE40_RS01825 at 361.989 kb on + strand, within ABIE40_RS01825 at 361.990 kb on - strand, within ABIE40_RS01825 at 361.990 kb on - strand, within ABIE40_RS01825 at 361.990 kb on - strand, within ABIE40_RS01825 at 361.990 kb on - strand, within ABIE40_RS01825 at 361.990 kb on - strand, within ABIE40_RS01825 at 361.990 kb on - strand, within ABIE40_RS01825 at 361.991 kb on + strand, within ABIE40_RS01825 at 362.067 kb on + strand, within ABIE40_RS01825 at 362.068 kb on - strand, within ABIE40_RS01825 at 362.068 kb on - strand, within ABIE40_RS01825 at 362.068 kb on - strand, within ABIE40_RS01825 at 362.068 kb on - strand, within ABIE40_RS01825 at 362.112 kb on + strand, within ABIE40_RS01825 at 362.112 kb on + strand, within ABIE40_RS01825 at 362.112 kb on + strand, within ABIE40_RS01825 at 362.112 kb on + strand, within ABIE40_RS01825 at 362.112 kb on + strand, within ABIE40_RS01825 at 362.112 kb on + strand, within ABIE40_RS01825 at 362.112 kb on + strand, within ABIE40_RS01825 at 362.112 kb on + strand, within ABIE40_RS01825 at 362.113 kb on - strand, within ABIE40_RS01825 at 362.113 kb on - strand, within ABIE40_RS01825 at 362.113 kb on - strand, within ABIE40_RS01825 at 362.113 kb on - strand, within ABIE40_RS01825 at 362.166 kb on + strand at 362.167 kb on - strand at 362.533 kb on + strand at 362.533 kb on + strand at 362.533 kb on + strand at 362.533 kb on + strand at 362.533 kb on + strand at 362.533 kb on + strand at 362.533 kb on + strand at 362.534 kb on - strand at 362.534 kb on - strand at 362.534 kb on - strand at 362.534 kb on - strand at 362.534 kb on - strand at 362.534 kb on - strand at 362.534 kb on - strand at 362.534 kb on - strand at 362.534 kb on - strand at 362.534 kb on - strand at 362.534 kb on - strand at 362.534 kb on - strand at 362.534 kb on - strand at 362.583 kb on + strand at 362.583 kb on + strand at 362.584 kb on - strand at 362.594 kb on + strand at 362.594 kb on + strand at 362.595 kb on - strand at 362.891 kb on + strand, within ABIE40_RS01835 at 362.918 kb on - strand at 362.956 kb on - strand at 363.184 kb on + strand, within ABIE40_RS01840 at 363.184 kb on + strand, within ABIE40_RS01840 at 363.253 kb on + strand, within ABIE40_RS01840 at 363.253 kb on + strand, within ABIE40_RS01840 at 363.253 kb on + strand, within ABIE40_RS01840 at 363.254 kb on - strand, within ABIE40_RS01840 at 363.254 kb on - strand, within ABIE40_RS01840 at 363.275 kb on + strand, within ABIE40_RS01840 at 363.275 kb on + strand, within ABIE40_RS01840 at 363.275 kb on + strand, within ABIE40_RS01840 at 363.275 kb on + strand, within ABIE40_RS01840 at 363.276 kb on - strand, within ABIE40_RS01840 at 363.276 kb on - strand, within ABIE40_RS01840 at 363.276 kb on - strand, within ABIE40_RS01840 at 363.334 kb on + strand, within ABIE40_RS01840 at 363.334 kb on + strand, within ABIE40_RS01840 at 363.334 kb on + strand, within ABIE40_RS01840 at 363.334 kb on + strand, within ABIE40_RS01840 at 363.334 kb on + strand, within ABIE40_RS01840 at 363.334 kb on + strand, within ABIE40_RS01840 at 363.334 kb on + strand, within ABIE40_RS01840 at 363.334 kb on + strand, within ABIE40_RS01840 at 363.334 kb on + strand, within ABIE40_RS01840 at 363.334 kb on + strand, within ABIE40_RS01840 at 363.335 kb on - strand, within ABIE40_RS01840 at 363.335 kb on - strand, within ABIE40_RS01840 at 363.335 kb on - strand, within ABIE40_RS01840 at 363.335 kb on - strand, within ABIE40_RS01840 at 363.335 kb on - strand, within ABIE40_RS01840 at 363.335 kb on - strand, within ABIE40_RS01840 at 363.335 kb on - strand, within ABIE40_RS01840 at 363.335 kb on - strand, within ABIE40_RS01840 at 363.383 kb on + strand, within ABIE40_RS01840 at 363.384 kb on - strand, within ABIE40_RS01840 at 363.441 kb on + strand, within ABIE40_RS01840 at 363.554 kb on + strand, within ABIE40_RS01840 at 363.554 kb on + strand, within ABIE40_RS01840 at 363.554 kb on + strand, within ABIE40_RS01840 at 363.554 kb on + strand, within ABIE40_RS01840 at 363.555 kb on - strand, within ABIE40_RS01840 at 363.555 kb on - strand, within ABIE40_RS01840 at 363.555 kb on - strand, within ABIE40_RS01840 at 363.555 kb on - strand, within ABIE40_RS01840 at 363.555 kb on - strand, within ABIE40_RS01840 at 363.555 kb on - strand, within ABIE40_RS01840 at 363.555 kb on - strand, within ABIE40_RS01840 at 363.555 kb on - strand, within ABIE40_RS01840 at 363.555 kb on - strand, within ABIE40_RS01840 at 363.555 kb on - strand, within ABIE40_RS01840 at 363.556 kb on + strand, within ABIE40_RS01840 at 363.557 kb on - strand, within ABIE40_RS01840 at 363.557 kb on - strand, within ABIE40_RS01840 at 363.557 kb on - strand, within ABIE40_RS01840 at 363.557 kb on - strand, within ABIE40_RS01840 at 363.557 kb on - strand, within ABIE40_RS01840 at 363.557 kb on - strand, within ABIE40_RS01840 at 363.578 kb on + strand, within ABIE40_RS01840 at 363.578 kb on + strand, within ABIE40_RS01840 at 363.578 kb on + strand, within ABIE40_RS01840 at 363.579 kb on - strand, within ABIE40_RS01840 at 363.649 kb on + strand, within ABIE40_RS01840 at 363.649 kb on + strand, within ABIE40_RS01840 at 363.650 kb on - strand, within ABIE40_RS01840 at 363.801 kb on - strand, within ABIE40_RS01840 at 363.821 kb on - strand, within ABIE40_RS01840 at 363.821 kb on - strand, within ABIE40_RS01840 at 363.821 kb on - strand, within ABIE40_RS01840 at 363.821 kb on - strand, within ABIE40_RS01840 at 363.923 kb on + strand at 363.923 kb on + strand at 363.923 kb on + strand at 363.923 kb on + strand at 363.923 kb on + strand at 363.923 kb on + strand at 363.923 kb on + strand at 363.924 kb on - strand at 363.924 kb on - strand at 363.924 kb on - strand at 363.924 kb on - strand at 363.924 kb on - strand at 363.970 kb on + strand at 363.970 kb on + strand at 363.970 kb on + strand at 363.971 kb on - strand at 363.971 kb on - strand at 364.058 kb on + strand at 364.059 kb on - strand at 364.059 kb on - strand at 364.063 kb on + strand at 364.063 kb on + strand at 364.063 kb on + strand at 364.063 kb on + strand at 364.066 kb on + strand at 364.066 kb on + strand at 364.096 kb on + strand at 364.103 kb on + strand at 364.103 kb on + strand at 364.103 kb on + strand at 364.103 kb on + strand at 364.105 kb on + strand at 364.107 kb on + strand at 364.107 kb on + strand at 364.107 kb on + strand at 364.107 kb on + strand at 364.107 kb on + strand at 364.107 kb on + strand at 364.107 kb on + strand at 364.107 kb on + strand at 364.107 kb on + strand at 364.107 kb on + strand at 364.107 kb on + strand at 364.804 kb on + strand at 364.804 kb on + strand at 364.804 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with 1-octen-3-ol 1.08545883333333 mM remove 361,989 + ABIE40_RS01825 0.51 -1.1 361,989 + ABIE40_RS01825 0.51 -1.6 361,989 + ABIE40_RS01825 0.51 -0.6 361,989 + ABIE40_RS01825 0.51 -2.5 361,989 + ABIE40_RS01825 0.51 +0.9 361,990 - ABIE40_RS01825 0.51 +0.3 361,990 - ABIE40_RS01825 0.51 -1.0 361,990 - ABIE40_RS01825 0.51 -4.8 361,990 - ABIE40_RS01825 0.51 -0.1 361,990 - ABIE40_RS01825 0.51 -0.2 361,990 - ABIE40_RS01825 0.51 -0.4 361,991 + ABIE40_RS01825 0.51 +1.3 362,067 + ABIE40_RS01825 0.68 +0.6 362,068 - ABIE40_RS01825 0.69 +2.2 362,068 - ABIE40_RS01825 0.69 +0.4 362,068 - ABIE40_RS01825 0.69 +0.0 362,068 - ABIE40_RS01825 0.69 -3.5 362,112 + ABIE40_RS01825 0.79 -1.2 362,112 + ABIE40_RS01825 0.79 -0.9 362,112 + ABIE40_RS01825 0.79 +0.1 362,112 + ABIE40_RS01825 0.79 -0.1 362,112 + ABIE40_RS01825 0.79 -2.5 362,112 + ABIE40_RS01825 0.79 -2.2 362,112 + ABIE40_RS01825 0.79 +0.5 362,112 + ABIE40_RS01825 0.79 -0.3 362,113 - ABIE40_RS01825 0.79 +1.6 362,113 - ABIE40_RS01825 0.79 +0.2 362,113 - ABIE40_RS01825 0.79 -0.1 362,113 - ABIE40_RS01825 0.79 -3.4 362,166 + -2.0 362,167 - -1.5 362,533 + -1.2 362,533 + -0.1 362,533 + +0.8 362,533 + -1.6 362,533 + -0.4 362,533 + -0.4 362,533 + +2.5 362,534 - +0.1 362,534 - -0.3 362,534 - -1.4 362,534 - -0.1 362,534 - -2.3 362,534 - -2.6 362,534 - +0.3 362,534 - +0.8 362,534 - +1.8 362,534 - -1.7 362,534 - -0.1 362,534 - +1.4 362,534 - -2.0 362,583 + +0.8 362,583 + -2.8 362,584 - -0.8 362,594 + -0.4 362,594 + +1.2 362,595 - -0.1 362,891 + ABIE40_RS01835 0.82 -1.5 362,918 - +3.0 362,956 - -2.5 363,184 + ABIE40_RS01840 0.22 +0.5 363,184 + ABIE40_RS01840 0.22 -0.9 363,253 + ABIE40_RS01840 0.29 +1.8 363,253 + ABIE40_RS01840 0.29 +0.0 363,253 + ABIE40_RS01840 0.29 -3.2 363,254 - ABIE40_RS01840 0.30 -0.4 363,254 - ABIE40_RS01840 0.30 -3.5 363,275 + ABIE40_RS01840 0.32 +0.6 363,275 + ABIE40_RS01840 0.32 -4.1 363,275 + ABIE40_RS01840 0.32 +0.9 363,275 + ABIE40_RS01840 0.32 +0.5 363,276 - ABIE40_RS01840 0.32 +0.6 363,276 - ABIE40_RS01840 0.32 -1.0 363,276 - ABIE40_RS01840 0.32 +1.3 363,334 + ABIE40_RS01840 0.38 -2.4 363,334 + ABIE40_RS01840 0.38 -1.5 363,334 + ABIE40_RS01840 0.38 -0.0 363,334 + ABIE40_RS01840 0.38 -2.8 363,334 + ABIE40_RS01840 0.38 -0.4 363,334 + ABIE40_RS01840 0.38 -0.2 363,334 + ABIE40_RS01840 0.38 +1.2 363,334 + ABIE40_RS01840 0.38 -3.5 363,334 + ABIE40_RS01840 0.38 +0.3 363,334 + ABIE40_RS01840 0.38 -1.1 363,335 - ABIE40_RS01840 0.38 +0.2 363,335 - ABIE40_RS01840 0.38 -0.1 363,335 - ABIE40_RS01840 0.38 -1.0 363,335 - ABIE40_RS01840 0.38 -0.6 363,335 - ABIE40_RS01840 0.38 -0.1 363,335 - ABIE40_RS01840 0.38 -1.6 363,335 - ABIE40_RS01840 0.38 -0.2 363,335 - ABIE40_RS01840 0.38 +0.7 363,383 + ABIE40_RS01840 0.43 +2.2 363,384 - ABIE40_RS01840 0.43 -1.9 363,441 + ABIE40_RS01840 0.49 -1.0 363,554 + ABIE40_RS01840 0.61 -3.1 363,554 + ABIE40_RS01840 0.61 -3.8 363,554 + ABIE40_RS01840 0.61 -0.4 363,554 + ABIE40_RS01840 0.61 +1.6 363,555 - ABIE40_RS01840 0.61 -2.0 363,555 - ABIE40_RS01840 0.61 -2.6 363,555 - ABIE40_RS01840 0.61 +1.0 363,555 - ABIE40_RS01840 0.61 -2.3 363,555 - ABIE40_RS01840 0.61 -0.1 363,555 - ABIE40_RS01840 0.61 -0.6 363,555 - ABIE40_RS01840 0.61 -2.9 363,555 - ABIE40_RS01840 0.61 -1.5 363,555 - ABIE40_RS01840 0.61 -1.0 363,555 - ABIE40_RS01840 0.61 -4.2 363,556 + ABIE40_RS01840 0.61 +1.1 363,557 - ABIE40_RS01840 0.61 -0.4 363,557 - ABIE40_RS01840 0.61 +0.6 363,557 - ABIE40_RS01840 0.61 +1.2 363,557 - ABIE40_RS01840 0.61 +0.2 363,557 - ABIE40_RS01840 0.61 -2.6 363,557 - ABIE40_RS01840 0.61 -1.7 363,578 + ABIE40_RS01840 0.64 -2.0 363,578 + ABIE40_RS01840 0.64 +0.2 363,578 + ABIE40_RS01840 0.64 -1.7 363,579 - ABIE40_RS01840 0.64 -1.1 363,649 + ABIE40_RS01840 0.71 -1.0 363,649 + ABIE40_RS01840 0.71 -0.2 363,650 - ABIE40_RS01840 0.71 -1.7 363,801 - ABIE40_RS01840 0.87 -0.2 363,821 - ABIE40_RS01840 0.89 -3.2 363,821 - ABIE40_RS01840 0.89 -2.4 363,821 - ABIE40_RS01840 0.89 +2.1 363,821 - ABIE40_RS01840 0.89 +1.5 363,923 + +0.4 363,923 + +2.9 363,923 + +1.5 363,923 + +0.2 363,923 + -0.4 363,923 + -2.4 363,923 + -2.0 363,924 - -3.1 363,924 - -4.1 363,924 - +1.2 363,924 - +0.2 363,924 - -1.7 363,970 + -0.4 363,970 + -0.4 363,970 + +0.1 363,971 - -1.7 363,971 - -0.5 364,058 + -2.8 364,059 - -2.0 364,059 - +0.2 364,063 + -1.7 364,063 + +1.6 364,063 + +0.7 364,063 + -1.2 364,066 + +1.2 364,066 + -2.4 364,096 + +1.5 364,103 + -2.4 364,103 + +2.2 364,103 + -0.1 364,103 + +0.8 364,105 + +2.5 364,107 + -1.8 364,107 + +0.2 364,107 + -0.2 364,107 + +0.5 364,107 + +0.4 364,107 + +1.0 364,107 + +1.9 364,107 + -2.4 364,107 + -3.9 364,107 + +0.6 364,107 + -0.2 364,804 + -0.9 364,804 + -2.5 364,804 + +2.5
Or see this region's nucleotide sequence