Experiment: R2A with 1-octen-3-ol 1.3568235 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE40_RS05370 and ABIE40_RS05375 are separated by 108 nucleotides ABIE40_RS05375 and ABIE40_RS05380 are separated by 4 nucleotides ABIE40_RS05380 and ABIE40_RS05385 overlap by 4 nucleotides
ABIE40_RS05370: ABIE40_RS05370 - polyprenyl synthetase family protein, at 1,051,707 to 1,052,723
_RS05370
ABIE40_RS05375: ABIE40_RS05375 - DUF2007 domain-containing protein, at 1,052,832 to 1,053,059
_RS05375
ABIE40_RS05380: ABIE40_RS05380 - methyltransferase, at 1,053,064 to 1,053,840
_RS05380
ABIE40_RS05385: ABIE40_RS05385 - Nramp family divalent metal transporter, at 1,053,837 to 1,055,177
_RS05385
Position (kb)
1052
1053
1054 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1052.761 kb on - strand at 1052.761 kb on - strand at 1052.801 kb on + strand at 1052.843 kb on + strand at 1052.843 kb on + strand at 1052.843 kb on + strand at 1052.844 kb on - strand at 1052.890 kb on + strand, within ABIE40_RS05375 at 1052.891 kb on - strand, within ABIE40_RS05375 at 1053.310 kb on + strand, within ABIE40_RS05380 at 1053.311 kb on - strand, within ABIE40_RS05380 at 1053.462 kb on + strand, within ABIE40_RS05380 at 1053.463 kb on - strand, within ABIE40_RS05380 at 1053.826 kb on + strand at 1053.826 kb on + strand at 1053.826 kb on + strand at 1053.826 kb on + strand at 1053.827 kb on - strand at 1053.827 kb on - strand at 1053.827 kb on - strand at 1053.827 kb on - strand at 1053.902 kb on - strand at 1054.055 kb on - strand, within ABIE40_RS05385
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with 1-octen-3-ol 1.3568235 mM remove 1,052,761 - -1.0 1,052,761 - +0.1 1,052,801 + +0.7 1,052,843 + -1.5 1,052,843 + +0.6 1,052,843 + -0.3 1,052,844 - -0.5 1,052,890 + ABIE40_RS05375 0.25 +0.8 1,052,891 - ABIE40_RS05375 0.26 -1.2 1,053,310 + ABIE40_RS05380 0.32 -1.9 1,053,311 - ABIE40_RS05380 0.32 -0.2 1,053,462 + ABIE40_RS05380 0.51 +0.9 1,053,463 - ABIE40_RS05380 0.51 +0.0 1,053,826 + +1.8 1,053,826 + +0.2 1,053,826 + -0.4 1,053,826 + -2.8 1,053,827 - -0.5 1,053,827 - +0.6 1,053,827 - +0.9 1,053,827 - -0.2 1,053,902 - +1.8 1,054,055 - ABIE40_RS05385 0.16 +0.8
Or see this region's nucleotide sequence