Experiment: R2A with 6-Pentyl-2H-pyran-2-one 0.716205166666667 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE40_RS18150 and ABIE40_RS18155 are separated by 114 nucleotides ABIE40_RS18155 and ABIE40_RS18160 overlap by 4 nucleotides ABIE40_RS18160 and ABIE40_RS18165 are separated by 84 nucleotides
ABIE40_RS18150: ABIE40_RS18150 - argininosuccinate synthase, at 3,629,796 to 3,631,019
_RS18150
ABIE40_RS18155: ABIE40_RS18155 - type II toxin-antitoxin system RelE/ParE family toxin, at 3,631,134 to 3,631,427
_RS18155
ABIE40_RS18160: ABIE40_RS18160 - type II toxin-antitoxin system ParD family antitoxin, at 3,631,424 to 3,631,666
_RS18160
ABIE40_RS18165: ABIE40_RS18165 - SDR family NAD(P)-dependent oxidoreductase, at 3,631,751 to 3,632,602
_RS18165
Position (kb)
3631
3632 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3631.040 kb on - strand at 3631.135 kb on + strand at 3631.136 kb on - strand at 3631.136 kb on - strand at 3631.181 kb on + strand, within ABIE40_RS18155 at 3631.181 kb on + strand, within ABIE40_RS18155 at 3631.182 kb on - strand, within ABIE40_RS18155 at 3631.184 kb on + strand, within ABIE40_RS18155 at 3631.184 kb on + strand, within ABIE40_RS18155 at 3631.184 kb on + strand, within ABIE40_RS18155 at 3631.185 kb on - strand, within ABIE40_RS18155 at 3631.185 kb on - strand, within ABIE40_RS18155 at 3631.185 kb on - strand, within ABIE40_RS18155 at 3631.185 kb on - strand, within ABIE40_RS18155 at 3631.185 kb on - strand, within ABIE40_RS18155 at 3631.185 kb on - strand, within ABIE40_RS18155 at 3631.185 kb on - strand, within ABIE40_RS18155 at 3631.243 kb on + strand, within ABIE40_RS18155 at 3631.243 kb on + strand, within ABIE40_RS18155 at 3631.243 kb on + strand, within ABIE40_RS18155 at 3631.244 kb on - strand, within ABIE40_RS18155 at 3631.244 kb on - strand, within ABIE40_RS18155 at 3631.327 kb on + strand, within ABIE40_RS18155 at 3631.327 kb on + strand, within ABIE40_RS18155 at 3631.327 kb on + strand, within ABIE40_RS18155 at 3631.328 kb on - strand, within ABIE40_RS18155 at 3631.328 kb on - strand, within ABIE40_RS18155 at 3631.328 kb on - strand, within ABIE40_RS18155 at 3631.328 kb on - strand, within ABIE40_RS18155 at 3631.328 kb on - strand, within ABIE40_RS18155 at 3631.330 kb on + strand, within ABIE40_RS18155 at 3631.330 kb on + strand, within ABIE40_RS18155 at 3631.330 kb on + strand, within ABIE40_RS18155 at 3631.331 kb on - strand, within ABIE40_RS18155 at 3631.331 kb on - strand, within ABIE40_RS18155 at 3631.331 kb on - strand, within ABIE40_RS18155 at 3631.355 kb on + strand, within ABIE40_RS18155 at 3631.355 kb on + strand, within ABIE40_RS18155 at 3631.355 kb on + strand, within ABIE40_RS18155 at 3631.356 kb on - strand, within ABIE40_RS18155 at 3631.356 kb on - strand, within ABIE40_RS18155 at 3631.356 kb on - strand, within ABIE40_RS18155 at 3631.356 kb on - strand, within ABIE40_RS18155 at 3631.366 kb on + strand, within ABIE40_RS18155 at 3631.367 kb on - strand, within ABIE40_RS18155 at 3631.372 kb on + strand, within ABIE40_RS18155 at 3631.372 kb on + strand, within ABIE40_RS18155 at 3631.372 kb on + strand, within ABIE40_RS18155 at 3631.372 kb on + strand, within ABIE40_RS18155 at 3631.372 kb on + strand, within ABIE40_RS18155 at 3631.373 kb on - strand, within ABIE40_RS18155 at 3631.373 kb on - strand, within ABIE40_RS18155 at 3631.373 kb on - strand, within ABIE40_RS18155 at 3631.373 kb on - strand, within ABIE40_RS18155 at 3631.373 kb on - strand, within ABIE40_RS18155 at 3631.373 kb on - strand, within ABIE40_RS18155 at 3631.373 kb on - strand, within ABIE40_RS18155 at 3631.374 kb on + strand, within ABIE40_RS18155 at 3631.374 kb on + strand, within ABIE40_RS18155 at 3631.374 kb on + strand, within ABIE40_RS18155 at 3631.374 kb on + strand, within ABIE40_RS18155 at 3631.375 kb on - strand, within ABIE40_RS18155 at 3631.375 kb on - strand, within ABIE40_RS18155 at 3631.454 kb on + strand, within ABIE40_RS18160 at 3631.590 kb on + strand, within ABIE40_RS18160 at 3631.590 kb on + strand, within ABIE40_RS18160 at 3631.590 kb on + strand, within ABIE40_RS18160 at 3631.590 kb on + strand, within ABIE40_RS18160 at 3631.590 kb on + strand, within ABIE40_RS18160 at 3631.590 kb on + strand, within ABIE40_RS18160 at 3631.591 kb on - strand, within ABIE40_RS18160 at 3631.591 kb on - strand, within ABIE40_RS18160 at 3631.591 kb on - strand, within ABIE40_RS18160 at 3631.591 kb on - strand, within ABIE40_RS18160 at 3631.591 kb on - strand, within ABIE40_RS18160 at 3631.591 kb on - strand, within ABIE40_RS18160 at 3631.591 kb on - strand, within ABIE40_RS18160 at 3631.591 kb on - strand, within ABIE40_RS18160 at 3631.591 kb on - strand, within ABIE40_RS18160 at 3631.591 kb on - strand, within ABIE40_RS18160 at 3631.703 kb on + strand at 3631.703 kb on + strand at 3631.703 kb on + strand at 3631.704 kb on - strand at 3631.704 kb on - strand at 3631.704 kb on - strand at 3631.709 kb on - strand at 3631.709 kb on - strand at 3631.768 kb on + strand at 3631.768 kb on + strand at 3631.769 kb on - strand at 3631.769 kb on - strand at 3631.771 kb on + strand at 3631.771 kb on + strand at 3631.772 kb on - strand at 3632.234 kb on + strand, within ABIE40_RS18165 at 3632.235 kb on - strand, within ABIE40_RS18165 at 3632.235 kb on - strand, within ABIE40_RS18165 at 3632.235 kb on - strand, within ABIE40_RS18165 at 3632.235 kb on - strand, within ABIE40_RS18165 at 3632.450 kb on + strand, within ABIE40_RS18165 at 3632.450 kb on + strand, within ABIE40_RS18165 at 3632.450 kb on + strand, within ABIE40_RS18165 at 3632.450 kb on + strand, within ABIE40_RS18165 at 3632.450 kb on + strand, within ABIE40_RS18165 at 3632.450 kb on + strand, within ABIE40_RS18165 at 3632.450 kb on + strand, within ABIE40_RS18165 at 3632.451 kb on - strand, within ABIE40_RS18165 at 3632.451 kb on - strand, within ABIE40_RS18165 at 3632.451 kb on - strand, within ABIE40_RS18165 at 3632.451 kb on - strand, within ABIE40_RS18165 at 3632.451 kb on - strand, within ABIE40_RS18165 at 3632.451 kb on - strand, within ABIE40_RS18165 at 3632.564 kb on + strand at 3632.565 kb on - strand at 3632.585 kb on + strand at 3632.585 kb on + strand at 3632.586 kb on - strand at 3632.600 kb on + strand at 3632.601 kb on - strand at 3632.601 kb on - strand at 3632.601 kb on - strand at 3632.640 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with 6-Pentyl-2H-pyran-2-one 0.716205166666667 mM remove 3,631,040 - +1.9 3,631,135 + -0.3 3,631,136 - -1.1 3,631,136 - +0.6 3,631,181 + ABIE40_RS18155 0.16 +1.5 3,631,181 + ABIE40_RS18155 0.16 +1.5 3,631,182 - ABIE40_RS18155 0.16 -2.3 3,631,184 + ABIE40_RS18155 0.17 -0.4 3,631,184 + ABIE40_RS18155 0.17 -0.7 3,631,184 + ABIE40_RS18155 0.17 -1.5 3,631,185 - ABIE40_RS18155 0.17 -1.1 3,631,185 - ABIE40_RS18155 0.17 -2.5 3,631,185 - ABIE40_RS18155 0.17 +2.1 3,631,185 - ABIE40_RS18155 0.17 -2.6 3,631,185 - ABIE40_RS18155 0.17 +0.7 3,631,185 - ABIE40_RS18155 0.17 -0.6 3,631,185 - ABIE40_RS18155 0.17 -0.9 3,631,243 + ABIE40_RS18155 0.37 -1.1 3,631,243 + ABIE40_RS18155 0.37 +1.1 3,631,243 + ABIE40_RS18155 0.37 +0.3 3,631,244 - ABIE40_RS18155 0.37 -0.1 3,631,244 - ABIE40_RS18155 0.37 -2.4 3,631,327 + ABIE40_RS18155 0.66 +1.3 3,631,327 + ABIE40_RS18155 0.66 -0.1 3,631,327 + ABIE40_RS18155 0.66 +0.9 3,631,328 - ABIE40_RS18155 0.66 -0.1 3,631,328 - ABIE40_RS18155 0.66 +0.9 3,631,328 - ABIE40_RS18155 0.66 +0.2 3,631,328 - ABIE40_RS18155 0.66 +1.9 3,631,328 - ABIE40_RS18155 0.66 -1.8 3,631,330 + ABIE40_RS18155 0.67 -0.7 3,631,330 + ABIE40_RS18155 0.67 +0.0 3,631,330 + ABIE40_RS18155 0.67 -0.5 3,631,331 - ABIE40_RS18155 0.67 -0.9 3,631,331 - ABIE40_RS18155 0.67 +0.7 3,631,331 - ABIE40_RS18155 0.67 -1.2 3,631,355 + ABIE40_RS18155 0.75 -1.3 3,631,355 + ABIE40_RS18155 0.75 -1.7 3,631,355 + ABIE40_RS18155 0.75 +0.4 3,631,356 - ABIE40_RS18155 0.76 +0.3 3,631,356 - ABIE40_RS18155 0.76 +0.5 3,631,356 - ABIE40_RS18155 0.76 -1.3 3,631,356 - ABIE40_RS18155 0.76 -0.4 3,631,366 + ABIE40_RS18155 0.79 +0.0 3,631,367 - ABIE40_RS18155 0.79 -0.6 3,631,372 + ABIE40_RS18155 0.81 -0.6 3,631,372 + ABIE40_RS18155 0.81 -1.4 3,631,372 + ABIE40_RS18155 0.81 +1.2 3,631,372 + ABIE40_RS18155 0.81 +1.9 3,631,372 + ABIE40_RS18155 0.81 -0.1 3,631,373 - ABIE40_RS18155 0.81 -1.4 3,631,373 - ABIE40_RS18155 0.81 +0.2 3,631,373 - ABIE40_RS18155 0.81 +0.7 3,631,373 - ABIE40_RS18155 0.81 -0.2 3,631,373 - ABIE40_RS18155 0.81 +0.7 3,631,373 - ABIE40_RS18155 0.81 -0.9 3,631,373 - ABIE40_RS18155 0.81 +0.8 3,631,374 + ABIE40_RS18155 0.82 -2.8 3,631,374 + ABIE40_RS18155 0.82 +0.9 3,631,374 + ABIE40_RS18155 0.82 +0.3 3,631,374 + ABIE40_RS18155 0.82 -2.4 3,631,375 - ABIE40_RS18155 0.82 +0.1 3,631,375 - ABIE40_RS18155 0.82 -0.1 3,631,454 + ABIE40_RS18160 0.12 +1.1 3,631,590 + ABIE40_RS18160 0.68 -2.0 3,631,590 + ABIE40_RS18160 0.68 +0.3 3,631,590 + ABIE40_RS18160 0.68 -0.2 3,631,590 + ABIE40_RS18160 0.68 -0.4 3,631,590 + ABIE40_RS18160 0.68 +0.0 3,631,590 + ABIE40_RS18160 0.68 -0.7 3,631,591 - ABIE40_RS18160 0.69 -0.8 3,631,591 - ABIE40_RS18160 0.69 +0.7 3,631,591 - ABIE40_RS18160 0.69 -1.1 3,631,591 - ABIE40_RS18160 0.69 -3.1 3,631,591 - ABIE40_RS18160 0.69 +1.0 3,631,591 - ABIE40_RS18160 0.69 -0.0 3,631,591 - ABIE40_RS18160 0.69 -1.1 3,631,591 - ABIE40_RS18160 0.69 -1.1 3,631,591 - ABIE40_RS18160 0.69 +0.9 3,631,591 - ABIE40_RS18160 0.69 -3.0 3,631,703 + -0.1 3,631,703 + +0.7 3,631,703 + -0.6 3,631,704 - -0.3 3,631,704 - +0.2 3,631,704 - -1.1 3,631,709 - -0.2 3,631,709 - +0.7 3,631,768 + +0.7 3,631,768 + -0.5 3,631,769 - -1.6 3,631,769 - -0.0 3,631,771 + -1.8 3,631,771 + -0.9 3,631,772 - -0.1 3,632,234 + ABIE40_RS18165 0.57 -0.4 3,632,235 - ABIE40_RS18165 0.57 +0.6 3,632,235 - ABIE40_RS18165 0.57 -0.5 3,632,235 - ABIE40_RS18165 0.57 +0.3 3,632,235 - ABIE40_RS18165 0.57 -1.1 3,632,450 + ABIE40_RS18165 0.82 +0.3 3,632,450 + ABIE40_RS18165 0.82 +0.7 3,632,450 + ABIE40_RS18165 0.82 -0.3 3,632,450 + ABIE40_RS18165 0.82 -0.7 3,632,450 + ABIE40_RS18165 0.82 -0.1 3,632,450 + ABIE40_RS18165 0.82 -2.3 3,632,450 + ABIE40_RS18165 0.82 +0.4 3,632,451 - ABIE40_RS18165 0.82 +1.1 3,632,451 - ABIE40_RS18165 0.82 +1.3 3,632,451 - ABIE40_RS18165 0.82 +1.3 3,632,451 - ABIE40_RS18165 0.82 -0.4 3,632,451 - ABIE40_RS18165 0.82 -2.6 3,632,451 - ABIE40_RS18165 0.82 -0.0 3,632,564 + -0.7 3,632,565 - -0.0 3,632,585 + +1.9 3,632,585 + +1.1 3,632,586 - -2.3 3,632,600 + -1.1 3,632,601 - -0.4 3,632,601 - +0.7 3,632,601 - -0.4 3,632,640 - +0.2
Or see this region's nucleotide sequence