Experiment: R2A with 6-Pentyl-2H-pyran-2-one 0.716205166666667 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE40_RS03515 and ABIE40_RS03520 are separated by 151 nucleotides ABIE40_RS03520 and ABIE40_RS03525 are separated by 14 nucleotides ABIE40_RS03525 and ABIE40_RS03530 are separated by 63 nucleotides ABIE40_RS03530 and ABIE40_RS03535 are separated by 260 nucleotides
ABIE40_RS03515: ABIE40_RS03515 - glutamate--cysteine ligase, at 697,161 to 698,534
_RS03515
ABIE40_RS03520: ABIE40_RS03520 - 16S rRNA (uracil(1498)-N(3))-methyltransferase, at 698,686 to 699,423
_RS03520
ABIE40_RS03525: ABIE40_RS03525 - inorganic phosphate transporter, at 699,438 to 700,937
_RS03525
ABIE40_RS03530: ABIE40_RS03530 - NUDIX hydrolase, at 701,001 to 701,513
_RS03530
ABIE40_RS03535: ABIE40_RS03535 - LysR substrate-binding domain-containing protein, at 701,774 to 702,655
_RS03535
Position (kb) 699
700
701 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 698.542 kb on + strand at 698.550 kb on - strand at 698.550 kb on - strand at 698.550 kb on - strand at 698.550 kb on - strand at 698.550 kb on - strand at 698.550 kb on - strand at 698.595 kb on + strand at 698.595 kb on + strand at 698.596 kb on - strand at 698.596 kb on - strand at 698.611 kb on + strand at 698.611 kb on + strand at 698.611 kb on + strand at 698.612 kb on - strand at 698.675 kb on + strand at 698.676 kb on - strand at 698.688 kb on - strand at 698.921 kb on + strand, within ABIE40_RS03520 at 698.921 kb on + strand, within ABIE40_RS03520 at 698.921 kb on + strand, within ABIE40_RS03520 at 698.921 kb on + strand, within ABIE40_RS03520 at 698.921 kb on + strand, within ABIE40_RS03520 at 698.922 kb on - strand, within ABIE40_RS03520 at 698.922 kb on - strand, within ABIE40_RS03520 at 698.922 kb on - strand, within ABIE40_RS03520 at 698.922 kb on - strand, within ABIE40_RS03520 at 698.922 kb on - strand, within ABIE40_RS03520 at 699.135 kb on - strand, within ABIE40_RS03520 at 699.135 kb on - strand, within ABIE40_RS03520 at 699.135 kb on - strand, within ABIE40_RS03520 at 699.170 kb on + strand, within ABIE40_RS03520 at 699.171 kb on - strand, within ABIE40_RS03520 at 699.171 kb on - strand, within ABIE40_RS03520 at 699.171 kb on - strand, within ABIE40_RS03520 at 699.312 kb on + strand, within ABIE40_RS03520 at 699.312 kb on + strand, within ABIE40_RS03520 at 699.313 kb on - strand, within ABIE40_RS03520 at 699.313 kb on - strand, within ABIE40_RS03520 at 699.313 kb on - strand, within ABIE40_RS03520 at 699.332 kb on + strand, within ABIE40_RS03520 at 699.332 kb on + strand, within ABIE40_RS03520 at 699.336 kb on + strand, within ABIE40_RS03520 at 699.336 kb on + strand, within ABIE40_RS03520 at 699.336 kb on + strand, within ABIE40_RS03520 at 699.336 kb on + strand, within ABIE40_RS03520 at 699.336 kb on + strand, within ABIE40_RS03520 at 699.336 kb on + strand, within ABIE40_RS03520 at 699.336 kb on + strand, within ABIE40_RS03520 at 699.336 kb on + strand, within ABIE40_RS03520 at 699.337 kb on - strand, within ABIE40_RS03520 at 699.337 kb on - strand, within ABIE40_RS03520 at 699.337 kb on - strand, within ABIE40_RS03520 at 699.337 kb on - strand, within ABIE40_RS03520 at 699.337 kb on - strand, within ABIE40_RS03520 at 699.337 kb on - strand, within ABIE40_RS03520 at 699.337 kb on - strand, within ABIE40_RS03520 at 699.337 kb on - strand, within ABIE40_RS03520 at 699.337 kb on - strand, within ABIE40_RS03520 at 699.337 kb on - strand, within ABIE40_RS03520 at 699.337 kb on - strand, within ABIE40_RS03520 at 699.338 kb on + strand, within ABIE40_RS03520 at 699.338 kb on + strand, within ABIE40_RS03520 at 699.338 kb on + strand, within ABIE40_RS03520 at 699.338 kb on + strand, within ABIE40_RS03520 at 699.339 kb on - strand, within ABIE40_RS03520 at 699.339 kb on - strand, within ABIE40_RS03520 at 699.339 kb on - strand, within ABIE40_RS03520 at 699.458 kb on + strand at 699.458 kb on + strand at 699.466 kb on + strand at 699.466 kb on + strand at 699.626 kb on + strand, within ABIE40_RS03525 at 699.626 kb on + strand, within ABIE40_RS03525 at 699.627 kb on - strand, within ABIE40_RS03525 at 699.627 kb on - strand, within ABIE40_RS03525 at 699.627 kb on - strand, within ABIE40_RS03525 at 699.627 kb on - strand, within ABIE40_RS03525 at 699.628 kb on + strand, within ABIE40_RS03525 at 699.628 kb on + strand, within ABIE40_RS03525 at 699.629 kb on - strand, within ABIE40_RS03525 at 699.629 kb on - strand, within ABIE40_RS03525 at 699.629 kb on - strand, within ABIE40_RS03525 at 699.658 kb on + strand, within ABIE40_RS03525 at 699.659 kb on - strand, within ABIE40_RS03525 at 699.659 kb on - strand, within ABIE40_RS03525 at 699.659 kb on - strand, within ABIE40_RS03525 at 699.704 kb on + strand, within ABIE40_RS03525 at 699.704 kb on + strand, within ABIE40_RS03525 at 699.704 kb on + strand, within ABIE40_RS03525 at 699.705 kb on - strand, within ABIE40_RS03525 at 699.863 kb on + strand, within ABIE40_RS03525 at 699.863 kb on + strand, within ABIE40_RS03525 at 700.192 kb on + strand, within ABIE40_RS03525 at 700.192 kb on + strand, within ABIE40_RS03525 at 700.192 kb on + strand, within ABIE40_RS03525 at 700.193 kb on - strand, within ABIE40_RS03525 at 700.193 kb on - strand, within ABIE40_RS03525 at 700.193 kb on - strand, within ABIE40_RS03525 at 700.199 kb on + strand, within ABIE40_RS03525 at 700.270 kb on + strand, within ABIE40_RS03525 at 700.270 kb on + strand, within ABIE40_RS03525 at 700.270 kb on + strand, within ABIE40_RS03525 at 700.270 kb on + strand, within ABIE40_RS03525 at 700.271 kb on - strand, within ABIE40_RS03525 at 700.271 kb on - strand, within ABIE40_RS03525 at 700.706 kb on + strand, within ABIE40_RS03525 at 700.718 kb on + strand, within ABIE40_RS03525 at 700.718 kb on + strand, within ABIE40_RS03525 at 700.718 kb on + strand, within ABIE40_RS03525 at 700.719 kb on - strand, within ABIE40_RS03525 at 700.719 kb on - strand, within ABIE40_RS03525 at 700.719 kb on - strand, within ABIE40_RS03525 at 700.719 kb on - strand, within ABIE40_RS03525 at 700.719 kb on - strand, within ABIE40_RS03525 at 700.719 kb on - strand, within ABIE40_RS03525 at 700.720 kb on + strand, within ABIE40_RS03525 at 700.721 kb on - strand, within ABIE40_RS03525 at 700.721 kb on - strand, within ABIE40_RS03525 at 700.721 kb on - strand, within ABIE40_RS03525 at 700.721 kb on - strand, within ABIE40_RS03525 at 700.721 kb on - strand, within ABIE40_RS03525 at 700.721 kb on - strand, within ABIE40_RS03525 at 700.777 kb on + strand, within ABIE40_RS03525 at 700.777 kb on + strand, within ABIE40_RS03525 at 700.777 kb on + strand, within ABIE40_RS03525 at 700.777 kb on + strand, within ABIE40_RS03525 at 700.777 kb on + strand, within ABIE40_RS03525 at 700.778 kb on - strand, within ABIE40_RS03525 at 700.778 kb on - strand, within ABIE40_RS03525 at 700.778 kb on - strand, within ABIE40_RS03525 at 700.877 kb on + strand at 700.877 kb on + strand at 700.877 kb on + strand at 700.877 kb on + strand at 700.878 kb on - strand at 700.878 kb on - strand at 700.878 kb on - strand at 700.878 kb on - strand at 700.878 kb on - strand at 700.878 kb on - strand at 700.879 kb on + strand at 700.879 kb on + strand at 700.879 kb on + strand at 700.879 kb on + strand at 700.879 kb on + strand at 700.879 kb on + strand at 700.880 kb on - strand at 700.880 kb on - strand at 700.880 kb on - strand at 700.880 kb on - strand at 700.880 kb on - strand at 700.880 kb on - strand at 700.880 kb on - strand at 700.880 kb on - strand at 700.880 kb on - strand at 700.880 kb on - strand at 700.880 kb on - strand at 700.880 kb on - strand at 700.880 kb on - strand at 700.880 kb on - strand at 700.914 kb on - strand at 700.965 kb on - strand at 700.965 kb on - strand at 700.980 kb on - strand at 700.980 kb on - strand at 700.980 kb on - strand at 701.051 kb on + strand at 701.191 kb on + strand, within ABIE40_RS03530 at 701.191 kb on + strand, within ABIE40_RS03530 at 701.191 kb on + strand, within ABIE40_RS03530 at 701.191 kb on + strand, within ABIE40_RS03530 at 701.191 kb on + strand, within ABIE40_RS03530 at 701.192 kb on - strand, within ABIE40_RS03530 at 701.192 kb on - strand, within ABIE40_RS03530 at 701.243 kb on - strand, within ABIE40_RS03530 at 701.248 kb on + strand, within ABIE40_RS03530 at 701.249 kb on - strand, within ABIE40_RS03530 at 701.249 kb on - strand, within ABIE40_RS03530 at 701.249 kb on - strand, within ABIE40_RS03530 at 701.299 kb on + strand, within ABIE40_RS03530 at 701.300 kb on - strand, within ABIE40_RS03530 at 701.324 kb on - strand, within ABIE40_RS03530 at 701.431 kb on + strand, within ABIE40_RS03530 at 701.431 kb on + strand, within ABIE40_RS03530 at 701.446 kb on + strand, within ABIE40_RS03530 at 701.446 kb on + strand, within ABIE40_RS03530 at 701.447 kb on - strand, within ABIE40_RS03530 at 701.447 kb on - strand, within ABIE40_RS03530 at 701.447 kb on - strand, within ABIE40_RS03530 at 701.447 kb on - strand, within ABIE40_RS03530 at 701.447 kb on - strand, within ABIE40_RS03530 at 701.493 kb on + strand at 701.557 kb on + strand at 701.558 kb on - strand at 701.558 kb on - strand at 701.596 kb on + strand at 701.596 kb on + strand at 701.597 kb on - strand at 701.597 kb on - strand at 701.624 kb on + strand at 701.624 kb on + strand at 701.625 kb on - strand at 701.625 kb on - strand at 701.625 kb on - strand at 701.830 kb on + strand at 701.830 kb on + strand at 701.831 kb on - strand at 701.831 kb on - strand at 701.831 kb on - strand at 701.906 kb on + strand, within ABIE40_RS03535 at 701.906 kb on + strand, within ABIE40_RS03535 at 701.906 kb on + strand, within ABIE40_RS03535
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with 6-Pentyl-2H-pyran-2-one 0.716205166666667 mM remove 698,542 + -1.3 698,550 - +1.0 698,550 - -0.1 698,550 - -0.8 698,550 - +0.5 698,550 - -1.0 698,550 - +1.8 698,595 + -0.2 698,595 + +1.6 698,596 - -1.7 698,596 - -1.4 698,611 + +0.9 698,611 + -0.7 698,611 + -2.5 698,612 - -2.1 698,675 + -1.1 698,676 - -1.1 698,688 - +2.2 698,921 + ABIE40_RS03520 0.32 -1.5 698,921 + ABIE40_RS03520 0.32 +2.3 698,921 + ABIE40_RS03520 0.32 +0.1 698,921 + ABIE40_RS03520 0.32 -0.2 698,921 + ABIE40_RS03520 0.32 -0.0 698,922 - ABIE40_RS03520 0.32 +1.3 698,922 - ABIE40_RS03520 0.32 +1.6 698,922 - ABIE40_RS03520 0.32 +0.7 698,922 - ABIE40_RS03520 0.32 +0.5 698,922 - ABIE40_RS03520 0.32 +0.6 699,135 - ABIE40_RS03520 0.61 +2.3 699,135 - ABIE40_RS03520 0.61 +0.5 699,135 - ABIE40_RS03520 0.61 +0.2 699,170 + ABIE40_RS03520 0.66 -0.4 699,171 - ABIE40_RS03520 0.66 +0.8 699,171 - ABIE40_RS03520 0.66 -2.0 699,171 - ABIE40_RS03520 0.66 +0.9 699,312 + ABIE40_RS03520 0.85 +0.2 699,312 + ABIE40_RS03520 0.85 +0.5 699,313 - ABIE40_RS03520 0.85 -0.1 699,313 - ABIE40_RS03520 0.85 +2.7 699,313 - ABIE40_RS03520 0.85 +1.3 699,332 + ABIE40_RS03520 0.88 -0.3 699,332 + ABIE40_RS03520 0.88 +0.3 699,336 + ABIE40_RS03520 0.88 +1.2 699,336 + ABIE40_RS03520 0.88 +1.1 699,336 + ABIE40_RS03520 0.88 +0.5 699,336 + ABIE40_RS03520 0.88 +0.3 699,336 + ABIE40_RS03520 0.88 +1.6 699,336 + ABIE40_RS03520 0.88 +1.5 699,336 + ABIE40_RS03520 0.88 +0.2 699,336 + ABIE40_RS03520 0.88 +0.1 699,337 - ABIE40_RS03520 0.88 +0.3 699,337 - ABIE40_RS03520 0.88 +1.6 699,337 - ABIE40_RS03520 0.88 +0.6 699,337 - ABIE40_RS03520 0.88 +0.9 699,337 - ABIE40_RS03520 0.88 +1.5 699,337 - ABIE40_RS03520 0.88 +0.9 699,337 - ABIE40_RS03520 0.88 +0.2 699,337 - ABIE40_RS03520 0.88 -0.3 699,337 - ABIE40_RS03520 0.88 -0.4 699,337 - ABIE40_RS03520 0.88 +0.7 699,337 - ABIE40_RS03520 0.88 +0.3 699,338 + ABIE40_RS03520 0.88 -0.3 699,338 + ABIE40_RS03520 0.88 +1.8 699,338 + ABIE40_RS03520 0.88 +0.7 699,338 + ABIE40_RS03520 0.88 +1.1 699,339 - ABIE40_RS03520 0.88 +0.2 699,339 - ABIE40_RS03520 0.88 -1.8 699,339 - ABIE40_RS03520 0.88 +0.5 699,458 + -0.2 699,458 + +1.6 699,466 + -0.3 699,466 + -1.6 699,626 + ABIE40_RS03525 0.13 +0.2 699,626 + ABIE40_RS03525 0.13 +0.9 699,627 - ABIE40_RS03525 0.13 +1.4 699,627 - ABIE40_RS03525 0.13 -0.7 699,627 - ABIE40_RS03525 0.13 +0.0 699,627 - ABIE40_RS03525 0.13 -1.2 699,628 + ABIE40_RS03525 0.13 +1.7 699,628 + ABIE40_RS03525 0.13 -0.0 699,629 - ABIE40_RS03525 0.13 +0.4 699,629 - ABIE40_RS03525 0.13 -1.7 699,629 - ABIE40_RS03525 0.13 -0.2 699,658 + ABIE40_RS03525 0.15 +0.4 699,659 - ABIE40_RS03525 0.15 +0.3 699,659 - ABIE40_RS03525 0.15 -1.6 699,659 - ABIE40_RS03525 0.15 -0.5 699,704 + ABIE40_RS03525 0.18 -2.2 699,704 + ABIE40_RS03525 0.18 -0.1 699,704 + ABIE40_RS03525 0.18 +0.4 699,705 - ABIE40_RS03525 0.18 -0.1 699,863 + ABIE40_RS03525 0.28 -1.3 699,863 + ABIE40_RS03525 0.28 +0.3 700,192 + ABIE40_RS03525 0.50 -1.4 700,192 + ABIE40_RS03525 0.50 -0.5 700,192 + ABIE40_RS03525 0.50 -0.4 700,193 - ABIE40_RS03525 0.50 -0.3 700,193 - ABIE40_RS03525 0.50 -0.1 700,193 - ABIE40_RS03525 0.50 +1.7 700,199 + ABIE40_RS03525 0.51 +2.1 700,270 + ABIE40_RS03525 0.55 -2.4 700,270 + ABIE40_RS03525 0.55 +0.5 700,270 + ABIE40_RS03525 0.55 -2.2 700,270 + ABIE40_RS03525 0.55 -0.2 700,271 - ABIE40_RS03525 0.56 +0.7 700,271 - ABIE40_RS03525 0.56 -1.1 700,706 + ABIE40_RS03525 0.85 +2.2 700,718 + ABIE40_RS03525 0.85 -0.4 700,718 + ABIE40_RS03525 0.85 +0.1 700,718 + ABIE40_RS03525 0.85 -1.2 700,719 - ABIE40_RS03525 0.85 +1.5 700,719 - ABIE40_RS03525 0.85 +0.0 700,719 - ABIE40_RS03525 0.85 +0.4 700,719 - ABIE40_RS03525 0.85 +0.8 700,719 - ABIE40_RS03525 0.85 +0.5 700,719 - ABIE40_RS03525 0.85 +0.5 700,720 + ABIE40_RS03525 0.85 +0.0 700,721 - ABIE40_RS03525 0.86 +0.3 700,721 - ABIE40_RS03525 0.86 +0.9 700,721 - ABIE40_RS03525 0.86 +0.3 700,721 - ABIE40_RS03525 0.86 +0.8 700,721 - ABIE40_RS03525 0.86 +0.2 700,721 - ABIE40_RS03525 0.86 +0.2 700,777 + ABIE40_RS03525 0.89 +1.0 700,777 + ABIE40_RS03525 0.89 -0.4 700,777 + ABIE40_RS03525 0.89 -0.6 700,777 + ABIE40_RS03525 0.89 -0.0 700,777 + ABIE40_RS03525 0.89 -1.4 700,778 - ABIE40_RS03525 0.89 +0.4 700,778 - ABIE40_RS03525 0.89 +0.1 700,778 - ABIE40_RS03525 0.89 +0.8 700,877 + +0.0 700,877 + +1.6 700,877 + +0.1 700,877 + +0.8 700,878 - +0.4 700,878 - -0.3 700,878 - -1.1 700,878 - +0.9 700,878 - +0.5 700,878 - -0.0 700,879 + -0.5 700,879 + +0.3 700,879 + +1.2 700,879 + -1.5 700,879 + -0.5 700,879 + +0.5 700,880 - -0.1 700,880 - +0.3 700,880 - +0.5 700,880 - -0.2 700,880 - +1.5 700,880 - -0.5 700,880 - -0.6 700,880 - +0.5 700,880 - +0.1 700,880 - -0.1 700,880 - +0.8 700,880 - +0.2 700,880 - +1.1 700,880 - +0.3 700,914 - +0.4 700,965 - +0.5 700,965 - +1.2 700,980 - +0.5 700,980 - +0.1 700,980 - +1.0 701,051 + -0.1 701,191 + ABIE40_RS03530 0.37 +0.8 701,191 + ABIE40_RS03530 0.37 +0.1 701,191 + ABIE40_RS03530 0.37 -0.4 701,191 + ABIE40_RS03530 0.37 +0.4 701,191 + ABIE40_RS03530 0.37 -0.0 701,192 - ABIE40_RS03530 0.37 +0.5 701,192 - ABIE40_RS03530 0.37 +0.0 701,243 - ABIE40_RS03530 0.47 -1.1 701,248 + ABIE40_RS03530 0.48 +0.2 701,249 - ABIE40_RS03530 0.48 -1.2 701,249 - ABIE40_RS03530 0.48 +0.9 701,249 - ABIE40_RS03530 0.48 +1.0 701,299 + ABIE40_RS03530 0.58 -0.7 701,300 - ABIE40_RS03530 0.58 +0.6 701,324 - ABIE40_RS03530 0.63 +0.7 701,431 + ABIE40_RS03530 0.84 -0.6 701,431 + ABIE40_RS03530 0.84 -1.1 701,446 + ABIE40_RS03530 0.87 -0.7 701,446 + ABIE40_RS03530 0.87 +0.4 701,447 - ABIE40_RS03530 0.87 -0.4 701,447 - ABIE40_RS03530 0.87 +2.0 701,447 - ABIE40_RS03530 0.87 +1.9 701,447 - ABIE40_RS03530 0.87 +0.9 701,447 - ABIE40_RS03530 0.87 +0.3 701,493 + +0.7 701,557 + -0.2 701,558 - -3.0 701,558 - -1.2 701,596 + -1.0 701,596 + +0.9 701,597 - -1.3 701,597 - -0.9 701,624 + +0.1 701,624 + +1.6 701,625 - -0.1 701,625 - -2.6 701,625 - -2.1 701,830 + +1.0 701,830 + +1.9 701,831 - -2.1 701,831 - -1.7 701,831 - +0.9 701,906 + ABIE40_RS03535 0.15 +0.6 701,906 + ABIE40_RS03535 0.15 +0.5 701,906 + ABIE40_RS03535 0.15 +0.5
Or see this region's nucleotide sequence