Experiment: R2A with 6-Pentyl-2H-pyran-2-one 0.716205166666667 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE40_RS12020 and ABIE40_RS12025 overlap by 4 nucleotides ABIE40_RS12025 and ABIE40_RS12030 are separated by 16 nucleotides ABIE40_RS12030 and ABIE40_RS12035 overlap by 8 nucleotides ABIE40_RS12035 and ABIE40_RS12040 are separated by 78 nucleotides
ABIE40_RS12020: ABIE40_RS12020 - iron-containing alcohol dehydrogenase, at 2,341,817 to 2,342,968
_RS12020
ABIE40_RS12025: ABIE40_RS12025 - aldehyde dehydrogenase family protein, at 2,342,965 to 2,344,350
_RS12025
ABIE40_RS12030: ABIE40_RS12030 - type II toxin-antitoxin system VapC family toxin, at 2,344,367 to 2,344,780
_RS12030
ABIE40_RS12035: ABIE40_RS12035 - helix-turn-helix domain-containing protein, at 2,344,773 to 2,345,249
_RS12035
ABIE40_RS12040: ABIE40_RS12040 - glutamine synthetase family protein, at 2,345,328 to 2,346,695
_RS12040
Position (kb)
2342
2343
2344
2345 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2341.993 kb on - strand, within ABIE40_RS12020 at 2341.993 kb on - strand, within ABIE40_RS12020 at 2341.998 kb on + strand, within ABIE40_RS12020 at 2341.998 kb on + strand, within ABIE40_RS12020 at 2341.998 kb on + strand, within ABIE40_RS12020 at 2341.999 kb on - strand, within ABIE40_RS12020 at 2341.999 kb on - strand, within ABIE40_RS12020 at 2341.999 kb on - strand, within ABIE40_RS12020 at 2341.999 kb on - strand, within ABIE40_RS12020 at 2341.999 kb on - strand, within ABIE40_RS12020 at 2341.999 kb on - strand, within ABIE40_RS12020 at 2342.032 kb on - strand, within ABIE40_RS12020 at 2342.032 kb on - strand, within ABIE40_RS12020 at 2342.032 kb on - strand, within ABIE40_RS12020 at 2342.130 kb on + strand, within ABIE40_RS12020 at 2342.130 kb on + strand, within ABIE40_RS12020 at 2342.130 kb on + strand, within ABIE40_RS12020 at 2342.131 kb on - strand, within ABIE40_RS12020 at 2342.251 kb on + strand, within ABIE40_RS12020 at 2342.251 kb on + strand, within ABIE40_RS12020 at 2342.252 kb on - strand, within ABIE40_RS12020 at 2342.253 kb on + strand, within ABIE40_RS12020 at 2342.254 kb on - strand, within ABIE40_RS12020 at 2342.322 kb on + strand, within ABIE40_RS12020 at 2342.322 kb on + strand, within ABIE40_RS12020 at 2342.322 kb on + strand, within ABIE40_RS12020 at 2342.322 kb on + strand, within ABIE40_RS12020 at 2342.322 kb on + strand, within ABIE40_RS12020 at 2342.322 kb on + strand, within ABIE40_RS12020 at 2342.323 kb on - strand, within ABIE40_RS12020 at 2342.323 kb on - strand, within ABIE40_RS12020 at 2342.323 kb on - strand, within ABIE40_RS12020 at 2342.323 kb on - strand, within ABIE40_RS12020 at 2342.323 kb on - strand, within ABIE40_RS12020 at 2342.337 kb on + strand, within ABIE40_RS12020 at 2342.337 kb on + strand, within ABIE40_RS12020 at 2342.337 kb on + strand, within ABIE40_RS12020 at 2342.338 kb on - strand, within ABIE40_RS12020 at 2342.938 kb on - strand at 2342.953 kb on + strand at 2342.953 kb on + strand at 2342.953 kb on + strand at 2342.953 kb on + strand at 2342.954 kb on - strand at 2342.954 kb on - strand at 2342.954 kb on - strand at 2342.954 kb on - strand at 2342.954 kb on - strand at 2342.954 kb on - strand at 2342.954 kb on - strand at 2342.954 kb on - strand at 2342.954 kb on - strand at 2342.991 kb on - strand at 2343.188 kb on + strand, within ABIE40_RS12025 at 2343.189 kb on - strand, within ABIE40_RS12025 at 2343.189 kb on - strand, within ABIE40_RS12025 at 2343.324 kb on - strand, within ABIE40_RS12025 at 2343.482 kb on + strand, within ABIE40_RS12025 at 2343.483 kb on - strand, within ABIE40_RS12025 at 2343.483 kb on - strand, within ABIE40_RS12025 at 2343.483 kb on - strand, within ABIE40_RS12025 at 2343.484 kb on - strand, within ABIE40_RS12025 at 2343.524 kb on + strand, within ABIE40_RS12025 at 2343.524 kb on + strand, within ABIE40_RS12025 at 2343.524 kb on + strand, within ABIE40_RS12025 at 2343.525 kb on - strand, within ABIE40_RS12025 at 2343.542 kb on + strand, within ABIE40_RS12025 at 2343.542 kb on + strand, within ABIE40_RS12025 at 2343.542 kb on + strand, within ABIE40_RS12025 at 2343.542 kb on + strand, within ABIE40_RS12025 at 2343.542 kb on + strand, within ABIE40_RS12025 at 2343.542 kb on + strand, within ABIE40_RS12025 at 2343.543 kb on - strand, within ABIE40_RS12025 at 2343.543 kb on - strand, within ABIE40_RS12025 at 2343.543 kb on - strand, within ABIE40_RS12025 at 2343.543 kb on - strand, within ABIE40_RS12025 at 2343.581 kb on + strand, within ABIE40_RS12025 at 2343.582 kb on - strand, within ABIE40_RS12025 at 2343.582 kb on - strand, within ABIE40_RS12025 at 2343.582 kb on - strand, within ABIE40_RS12025 at 2343.603 kb on + strand, within ABIE40_RS12025 at 2343.641 kb on + strand, within ABIE40_RS12025 at 2343.641 kb on + strand, within ABIE40_RS12025 at 2343.642 kb on - strand, within ABIE40_RS12025 at 2343.642 kb on - strand, within ABIE40_RS12025 at 2343.900 kb on + strand, within ABIE40_RS12025 at 2343.947 kb on + strand, within ABIE40_RS12025 at 2343.947 kb on + strand, within ABIE40_RS12025 at 2343.947 kb on + strand, within ABIE40_RS12025 at 2343.948 kb on - strand, within ABIE40_RS12025 at 2343.948 kb on - strand, within ABIE40_RS12025 at 2343.948 kb on - strand, within ABIE40_RS12025 at 2343.948 kb on - strand, within ABIE40_RS12025 at 2343.953 kb on + strand, within ABIE40_RS12025 at 2343.953 kb on + strand, within ABIE40_RS12025 at 2343.954 kb on - strand, within ABIE40_RS12025 at 2344.002 kb on + strand, within ABIE40_RS12025 at 2344.003 kb on - strand, within ABIE40_RS12025 at 2344.073 kb on + strand, within ABIE40_RS12025 at 2344.073 kb on + strand, within ABIE40_RS12025 at 2344.100 kb on + strand, within ABIE40_RS12025 at 2344.100 kb on + strand, within ABIE40_RS12025 at 2344.307 kb on + strand at 2344.331 kb on - strand at 2344.342 kb on - strand at 2344.342 kb on - strand at 2344.342 kb on - strand at 2344.342 kb on - strand at 2344.344 kb on + strand at 2344.344 kb on + strand at 2344.344 kb on + strand at 2344.344 kb on + strand at 2344.345 kb on - strand at 2344.345 kb on - strand at 2344.345 kb on - strand at 2344.345 kb on - strand at 2344.345 kb on - strand at 2344.345 kb on - strand at 2344.345 kb on - strand at 2344.355 kb on + strand at 2344.355 kb on + strand at 2344.356 kb on - strand at 2344.369 kb on - strand at 2344.369 kb on - strand at 2344.404 kb on + strand at 2344.564 kb on + strand, within ABIE40_RS12030 at 2344.564 kb on + strand, within ABIE40_RS12030 at 2344.565 kb on - strand, within ABIE40_RS12030 at 2344.565 kb on - strand, within ABIE40_RS12030 at 2344.565 kb on - strand, within ABIE40_RS12030 at 2344.565 kb on - strand, within ABIE40_RS12030 at 2344.592 kb on - strand, within ABIE40_RS12030 at 2344.592 kb on - strand, within ABIE40_RS12030 at 2344.631 kb on + strand, within ABIE40_RS12030 at 2344.631 kb on + strand, within ABIE40_RS12030 at 2344.631 kb on + strand, within ABIE40_RS12030 at 2344.631 kb on + strand, within ABIE40_RS12030 at 2344.631 kb on + strand, within ABIE40_RS12030 at 2344.631 kb on + strand, within ABIE40_RS12030 at 2344.631 kb on + strand, within ABIE40_RS12030 at 2344.632 kb on - strand, within ABIE40_RS12030 at 2344.632 kb on - strand, within ABIE40_RS12030 at 2344.632 kb on - strand, within ABIE40_RS12030 at 2344.632 kb on - strand, within ABIE40_RS12030 at 2344.632 kb on - strand, within ABIE40_RS12030 at 2344.633 kb on + strand, within ABIE40_RS12030 at 2344.634 kb on - strand, within ABIE40_RS12030 at 2344.635 kb on - strand, within ABIE40_RS12030 at 2344.672 kb on + strand, within ABIE40_RS12030 at 2344.694 kb on - strand, within ABIE40_RS12030 at 2344.719 kb on - strand, within ABIE40_RS12030 at 2344.756 kb on + strand at 2344.756 kb on + strand at 2344.756 kb on + strand at 2344.757 kb on - strand at 2344.757 kb on - strand at 2344.757 kb on - strand at 2344.786 kb on + strand at 2344.793 kb on + strand at 2344.930 kb on + strand, within ABIE40_RS12035 at 2344.930 kb on + strand, within ABIE40_RS12035 at 2344.930 kb on + strand, within ABIE40_RS12035 at 2344.949 kb on + strand, within ABIE40_RS12035 at 2344.949 kb on + strand, within ABIE40_RS12035 at 2344.949 kb on + strand, within ABIE40_RS12035 at 2345.084 kb on + strand, within ABIE40_RS12035 at 2345.084 kb on + strand, within ABIE40_RS12035 at 2345.085 kb on - strand, within ABIE40_RS12035 at 2345.086 kb on + strand, within ABIE40_RS12035 at 2345.086 kb on + strand, within ABIE40_RS12035 at 2345.087 kb on - strand, within ABIE40_RS12035 at 2345.183 kb on + strand, within ABIE40_RS12035 at 2345.281 kb on - strand at 2345.281 kb on - strand at 2345.281 kb on - strand at 2345.285 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with 6-Pentyl-2H-pyran-2-one 0.716205166666667 mM remove 2,341,993 - ABIE40_RS12020 0.15 +0.7 2,341,993 - ABIE40_RS12020 0.15 -0.7 2,341,998 + ABIE40_RS12020 0.16 +1.6 2,341,998 + ABIE40_RS12020 0.16 +1.2 2,341,998 + ABIE40_RS12020 0.16 +0.5 2,341,999 - ABIE40_RS12020 0.16 -0.8 2,341,999 - ABIE40_RS12020 0.16 +1.2 2,341,999 - ABIE40_RS12020 0.16 +0.4 2,341,999 - ABIE40_RS12020 0.16 +0.6 2,341,999 - ABIE40_RS12020 0.16 +0.4 2,341,999 - ABIE40_RS12020 0.16 +0.9 2,342,032 - ABIE40_RS12020 0.19 +0.1 2,342,032 - ABIE40_RS12020 0.19 +0.8 2,342,032 - ABIE40_RS12020 0.19 +0.1 2,342,130 + ABIE40_RS12020 0.27 -0.0 2,342,130 + ABIE40_RS12020 0.27 -0.1 2,342,130 + ABIE40_RS12020 0.27 +0.4 2,342,131 - ABIE40_RS12020 0.27 +0.4 2,342,251 + ABIE40_RS12020 0.38 -0.8 2,342,251 + ABIE40_RS12020 0.38 +0.2 2,342,252 - ABIE40_RS12020 0.38 -0.1 2,342,253 + ABIE40_RS12020 0.38 +1.3 2,342,254 - ABIE40_RS12020 0.38 -0.5 2,342,322 + ABIE40_RS12020 0.44 +0.4 2,342,322 + ABIE40_RS12020 0.44 +0.2 2,342,322 + ABIE40_RS12020 0.44 +0.2 2,342,322 + ABIE40_RS12020 0.44 +0.6 2,342,322 + ABIE40_RS12020 0.44 +1.7 2,342,322 + ABIE40_RS12020 0.44 -0.2 2,342,323 - ABIE40_RS12020 0.44 -1.3 2,342,323 - ABIE40_RS12020 0.44 +0.7 2,342,323 - ABIE40_RS12020 0.44 +0.1 2,342,323 - ABIE40_RS12020 0.44 -1.7 2,342,323 - ABIE40_RS12020 0.44 +0.1 2,342,337 + ABIE40_RS12020 0.45 -1.0 2,342,337 + ABIE40_RS12020 0.45 +1.5 2,342,337 + ABIE40_RS12020 0.45 -0.4 2,342,338 - ABIE40_RS12020 0.45 +1.0 2,342,938 - +0.5 2,342,953 + -1.5 2,342,953 + +0.6 2,342,953 + -0.1 2,342,953 + -0.4 2,342,954 - -0.9 2,342,954 - -0.9 2,342,954 - +1.9 2,342,954 - +0.3 2,342,954 - +0.3 2,342,954 - -0.9 2,342,954 - -0.8 2,342,954 - -0.5 2,342,954 - +0.2 2,342,991 - -0.3 2,343,188 + ABIE40_RS12025 0.16 +0.6 2,343,189 - ABIE40_RS12025 0.16 -0.4 2,343,189 - ABIE40_RS12025 0.16 -0.6 2,343,324 - ABIE40_RS12025 0.26 +1.2 2,343,482 + ABIE40_RS12025 0.37 -0.9 2,343,483 - ABIE40_RS12025 0.37 -0.5 2,343,483 - ABIE40_RS12025 0.37 +0.9 2,343,483 - ABIE40_RS12025 0.37 -0.2 2,343,484 - ABIE40_RS12025 0.37 -1.2 2,343,524 + ABIE40_RS12025 0.40 -2.3 2,343,524 + ABIE40_RS12025 0.40 -2.0 2,343,524 + ABIE40_RS12025 0.40 -0.3 2,343,525 - ABIE40_RS12025 0.40 -0.3 2,343,542 + ABIE40_RS12025 0.42 -2.1 2,343,542 + ABIE40_RS12025 0.42 -1.1 2,343,542 + ABIE40_RS12025 0.42 -0.5 2,343,542 + ABIE40_RS12025 0.42 -0.7 2,343,542 + ABIE40_RS12025 0.42 +0.9 2,343,542 + ABIE40_RS12025 0.42 -0.4 2,343,543 - ABIE40_RS12025 0.42 +0.4 2,343,543 - ABIE40_RS12025 0.42 -0.3 2,343,543 - ABIE40_RS12025 0.42 -0.9 2,343,543 - ABIE40_RS12025 0.42 +0.4 2,343,581 + ABIE40_RS12025 0.44 -1.7 2,343,582 - ABIE40_RS12025 0.45 +0.6 2,343,582 - ABIE40_RS12025 0.45 +0.1 2,343,582 - ABIE40_RS12025 0.45 -0.6 2,343,603 + ABIE40_RS12025 0.46 -0.1 2,343,641 + ABIE40_RS12025 0.49 +0.4 2,343,641 + ABIE40_RS12025 0.49 +0.9 2,343,642 - ABIE40_RS12025 0.49 -0.1 2,343,642 - ABIE40_RS12025 0.49 -0.7 2,343,900 + ABIE40_RS12025 0.67 +0.9 2,343,947 + ABIE40_RS12025 0.71 +0.6 2,343,947 + ABIE40_RS12025 0.71 -0.0 2,343,947 + ABIE40_RS12025 0.71 -0.1 2,343,948 - ABIE40_RS12025 0.71 +1.2 2,343,948 - ABIE40_RS12025 0.71 -1.3 2,343,948 - ABIE40_RS12025 0.71 -0.4 2,343,948 - ABIE40_RS12025 0.71 +0.3 2,343,953 + ABIE40_RS12025 0.71 +0.7 2,343,953 + ABIE40_RS12025 0.71 +0.7 2,343,954 - ABIE40_RS12025 0.71 -1.1 2,344,002 + ABIE40_RS12025 0.75 -0.2 2,344,003 - ABIE40_RS12025 0.75 +0.3 2,344,073 + ABIE40_RS12025 0.80 +0.1 2,344,073 + ABIE40_RS12025 0.80 +0.1 2,344,100 + ABIE40_RS12025 0.82 -0.8 2,344,100 + ABIE40_RS12025 0.82 -0.7 2,344,307 + +1.1 2,344,331 - -0.8 2,344,342 - +0.0 2,344,342 - -0.7 2,344,342 - +0.3 2,344,342 - -0.4 2,344,344 + +0.4 2,344,344 + +0.9 2,344,344 + +0.7 2,344,344 + +1.0 2,344,345 - -0.0 2,344,345 - +0.7 2,344,345 - -1.2 2,344,345 - +2.5 2,344,345 - +0.4 2,344,345 - -1.4 2,344,345 - -0.4 2,344,355 + -0.7 2,344,355 + +0.0 2,344,356 - +0.2 2,344,369 - +0.1 2,344,369 - +0.3 2,344,404 + -0.5 2,344,564 + ABIE40_RS12030 0.48 +1.1 2,344,564 + ABIE40_RS12030 0.48 -0.5 2,344,565 - ABIE40_RS12030 0.48 +1.2 2,344,565 - ABIE40_RS12030 0.48 -0.3 2,344,565 - ABIE40_RS12030 0.48 -0.1 2,344,565 - ABIE40_RS12030 0.48 +0.2 2,344,592 - ABIE40_RS12030 0.54 +0.3 2,344,592 - ABIE40_RS12030 0.54 +1.5 2,344,631 + ABIE40_RS12030 0.64 -1.3 2,344,631 + ABIE40_RS12030 0.64 +0.1 2,344,631 + ABIE40_RS12030 0.64 +0.0 2,344,631 + ABIE40_RS12030 0.64 -0.5 2,344,631 + ABIE40_RS12030 0.64 +0.3 2,344,631 + ABIE40_RS12030 0.64 +0.1 2,344,631 + ABIE40_RS12030 0.64 +0.5 2,344,632 - ABIE40_RS12030 0.64 -0.1 2,344,632 - ABIE40_RS12030 0.64 +0.6 2,344,632 - ABIE40_RS12030 0.64 -0.4 2,344,632 - ABIE40_RS12030 0.64 +0.0 2,344,632 - ABIE40_RS12030 0.64 +0.6 2,344,633 + ABIE40_RS12030 0.64 -1.4 2,344,634 - ABIE40_RS12030 0.64 -0.7 2,344,635 - ABIE40_RS12030 0.65 -0.7 2,344,672 + ABIE40_RS12030 0.74 -0.7 2,344,694 - ABIE40_RS12030 0.79 +0.0 2,344,719 - ABIE40_RS12030 0.85 +1.6 2,344,756 + -0.2 2,344,756 + -0.4 2,344,756 + +0.9 2,344,757 - +0.4 2,344,757 - +0.4 2,344,757 - -0.4 2,344,786 + +0.3 2,344,793 + -0.4 2,344,930 + ABIE40_RS12035 0.33 +0.9 2,344,930 + ABIE40_RS12035 0.33 +0.1 2,344,930 + ABIE40_RS12035 0.33 +0.7 2,344,949 + ABIE40_RS12035 0.37 -1.9 2,344,949 + ABIE40_RS12035 0.37 -0.1 2,344,949 + ABIE40_RS12035 0.37 -0.7 2,345,084 + ABIE40_RS12035 0.65 +0.1 2,345,084 + ABIE40_RS12035 0.65 -0.0 2,345,085 - ABIE40_RS12035 0.65 -2.1 2,345,086 + ABIE40_RS12035 0.66 -0.6 2,345,086 + ABIE40_RS12035 0.66 -0.2 2,345,087 - ABIE40_RS12035 0.66 +0.3 2,345,183 + ABIE40_RS12035 0.86 -1.1 2,345,281 - +0.5 2,345,281 - +0.7 2,345,281 - -0.7 2,345,285 + -0.8
Or see this region's nucleotide sequence