Experiment: R2A with 6-Pentyl-2H-pyran-2-one 0.716205166666667 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE40_RS27285 and ABIE40_RS27290 are separated by 182 nucleotides ABIE40_RS27290 and ABIE40_RS27295 are separated by 179 nucleotides ABIE40_RS27295 and ABIE40_RS27300 are separated by 5 nucleotides
ABIE40_RS27285: ABIE40_RS27285 - cold-shock protein, at 175,683 to 175,886
_RS27285
ABIE40_RS27290: ABIE40_RS27290 - hypothetical protein, at 176,069 to 176,521
_RS27290
ABIE40_RS27295: ABIE40_RS27295 - hypothetical protein, at 176,701 to 177,024
_RS27295
ABIE40_RS27300: ABIE40_RS27300 - YihY/virulence factor BrkB family protein, at 177,030 to 178,112
_RS27300
Position (kb)
176
177 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 175.171 kb on - strand at 175.224 kb on + strand at 175.225 kb on - strand at 175.268 kb on + strand at 175.269 kb on - strand at 175.283 kb on - strand at 175.297 kb on - strand at 175.297 kb on - strand at 175.301 kb on + strand at 175.301 kb on + strand at 175.302 kb on - strand at 175.366 kb on - strand at 175.386 kb on - strand at 175.460 kb on + strand at 175.460 kb on + strand at 175.461 kb on - strand at 175.461 kb on - strand at 175.567 kb on + strand at 175.567 kb on + strand at 175.567 kb on + strand at 175.567 kb on + strand at 175.643 kb on + strand at 175.644 kb on - strand at 175.652 kb on + strand at 175.668 kb on + strand at 175.824 kb on + strand, within ABIE40_RS27285 at 175.824 kb on + strand, within ABIE40_RS27285 at 175.825 kb on - strand, within ABIE40_RS27285 at 175.825 kb on - strand, within ABIE40_RS27285 at 175.844 kb on + strand, within ABIE40_RS27285 at 175.936 kb on + strand at 175.937 kb on - strand at 175.961 kb on + strand at 175.961 kb on + strand at 175.961 kb on + strand at 175.962 kb on - strand at 175.962 kb on - strand at 176.007 kb on + strand at 176.063 kb on + strand at 176.063 kb on + strand at 176.063 kb on + strand at 176.064 kb on - strand at 176.064 kb on - strand at 176.064 kb on - strand at 176.064 kb on - strand at 176.216 kb on + strand, within ABIE40_RS27290 at 176.216 kb on + strand, within ABIE40_RS27290 at 176.216 kb on + strand, within ABIE40_RS27290 at 176.217 kb on - strand, within ABIE40_RS27290 at 176.217 kb on - strand, within ABIE40_RS27290 at 176.346 kb on - strand, within ABIE40_RS27290 at 176.346 kb on - strand, within ABIE40_RS27290 at 176.413 kb on + strand, within ABIE40_RS27290 at 176.414 kb on - strand, within ABIE40_RS27290 at 176.421 kb on - strand, within ABIE40_RS27290 at 176.524 kb on - strand at 176.638 kb on + strand at 176.638 kb on + strand at 176.638 kb on + strand at 176.638 kb on + strand at 176.639 kb on - strand at 176.639 kb on - strand at 176.639 kb on - strand at 176.878 kb on + strand, within ABIE40_RS27295 at 176.878 kb on + strand, within ABIE40_RS27295 at 176.932 kb on - strand, within ABIE40_RS27295 at 177.019 kb on - strand at 177.150 kb on - strand, within ABIE40_RS27300 at 177.229 kb on + strand, within ABIE40_RS27300 at 177.229 kb on + strand, within ABIE40_RS27300 at 177.229 kb on + strand, within ABIE40_RS27300 at 177.229 kb on + strand, within ABIE40_RS27300 at 177.230 kb on - strand, within ABIE40_RS27300 at 177.230 kb on - strand, within ABIE40_RS27300 at 177.230 kb on - strand, within ABIE40_RS27300 at 177.242 kb on - strand, within ABIE40_RS27300 at 177.263 kb on - strand, within ABIE40_RS27300 at 177.417 kb on + strand, within ABIE40_RS27300 at 177.418 kb on - strand, within ABIE40_RS27300 at 177.460 kb on + strand, within ABIE40_RS27300 at 177.460 kb on + strand, within ABIE40_RS27300 at 177.461 kb on - strand, within ABIE40_RS27300 at 177.461 kb on - strand, within ABIE40_RS27300 at 177.461 kb on - strand, within ABIE40_RS27300
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with 6-Pentyl-2H-pyran-2-one 0.716205166666667 mM remove 175,171 - +2.5 175,224 + +3.8 175,225 - +2.1 175,268 + +1.9 175,269 - +1.2 175,283 - -0.7 175,297 - -0.3 175,297 - +0.5 175,301 + +0.2 175,301 + +2.5 175,302 - -0.0 175,366 - +0.9 175,386 - +1.0 175,460 + -0.1 175,460 + +2.9 175,461 - -2.1 175,461 - +1.6 175,567 + -1.1 175,567 + +0.7 175,567 + +2.9 175,567 + +0.2 175,643 + +2.5 175,644 - -0.3 175,652 + +1.4 175,668 + +1.1 175,824 + ABIE40_RS27285 0.69 -0.2 175,824 + ABIE40_RS27285 0.69 +0.5 175,825 - ABIE40_RS27285 0.70 +0.8 175,825 - ABIE40_RS27285 0.70 +0.2 175,844 + ABIE40_RS27285 0.79 +0.5 175,936 + -0.1 175,937 - +0.2 175,961 + +1.9 175,961 + +1.5 175,961 + +1.3 175,962 - -2.4 175,962 - -1.1 176,007 + +0.2 176,063 + -0.5 176,063 + +0.9 176,063 + +0.2 176,064 - +0.8 176,064 - +1.5 176,064 - -0.5 176,064 - +0.6 176,216 + ABIE40_RS27290 0.32 +0.3 176,216 + ABIE40_RS27290 0.32 +1.6 176,216 + ABIE40_RS27290 0.32 +0.6 176,217 - ABIE40_RS27290 0.33 -2.4 176,217 - ABIE40_RS27290 0.33 +1.6 176,346 - ABIE40_RS27290 0.61 +0.8 176,346 - ABIE40_RS27290 0.61 -0.3 176,413 + ABIE40_RS27290 0.76 +0.2 176,414 - ABIE40_RS27290 0.76 +0.6 176,421 - ABIE40_RS27290 0.78 +0.3 176,524 - +0.9 176,638 + -1.5 176,638 + -1.0 176,638 + +0.9 176,638 + -0.8 176,639 - -0.4 176,639 - +0.6 176,639 - -0.1 176,878 + ABIE40_RS27295 0.55 +0.9 176,878 + ABIE40_RS27295 0.55 -0.1 176,932 - ABIE40_RS27295 0.71 -0.5 177,019 - +1.5 177,150 - ABIE40_RS27300 0.11 -1.8 177,229 + ABIE40_RS27300 0.18 +2.2 177,229 + ABIE40_RS27300 0.18 +0.6 177,229 + ABIE40_RS27300 0.18 +0.5 177,229 + ABIE40_RS27300 0.18 +0.5 177,230 - ABIE40_RS27300 0.18 +2.0 177,230 - ABIE40_RS27300 0.18 +0.7 177,230 - ABIE40_RS27300 0.18 -1.3 177,242 - ABIE40_RS27300 0.20 +2.5 177,263 - ABIE40_RS27300 0.22 +2.2 177,417 + ABIE40_RS27300 0.36 +0.2 177,418 - ABIE40_RS27300 0.36 +0.2 177,460 + ABIE40_RS27300 0.40 +0.9 177,460 + ABIE40_RS27300 0.40 -2.8 177,461 - ABIE40_RS27300 0.40 +1.1 177,461 - ABIE40_RS27300 0.40 -1.3 177,461 - ABIE40_RS27300 0.40 -0.3
Or see this region's nucleotide sequence