Experiment: R2A with 6-Pentyl-2H-pyran-2-one 0.835572666666667 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE40_RS29910 and ABIE40_RS29915 overlap by 4 nucleotides ABIE40_RS29915 and ABIE40_RS29920 are separated by 500 nucleotides ABIE40_RS29920 and ABIE40_RS29925 overlap by 14 nucleotides
ABIE40_RS29910: ABIE40_RS29910 - hypothetical protein, at 674,123 to 674,458
_RS29910
ABIE40_RS29915: ABIE40_RS29915 - hypothetical protein, at 674,455 to 674,814
_RS29915
ABIE40_RS29920: ABIE40_RS29920 - type IV toxin-antitoxin system AbiEi family antitoxin, at 675,315 to 676,106
_RS29920
ABIE40_RS29925: ABIE40_RS29925 - nucleotidyl transferase AbiEii/AbiGii toxin family protein, at 676,093 to 677,010
_RS29925
Position (kb) 675
676
677 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 674.404 kb on - strand, within ABIE40_RS29910 at 674.404 kb on - strand, within ABIE40_RS29910 at 674.404 kb on - strand, within ABIE40_RS29910 at 674.404 kb on - strand, within ABIE40_RS29910 at 674.559 kb on + strand, within ABIE40_RS29915 at 674.559 kb on + strand, within ABIE40_RS29915 at 674.592 kb on + strand, within ABIE40_RS29915 at 674.592 kb on + strand, within ABIE40_RS29915 at 674.592 kb on + strand, within ABIE40_RS29915 at 674.593 kb on - strand, within ABIE40_RS29915 at 674.593 kb on - strand, within ABIE40_RS29915 at 674.593 kb on - strand, within ABIE40_RS29915 at 674.692 kb on + strand, within ABIE40_RS29915 at 674.693 kb on - strand, within ABIE40_RS29915 at 674.952 kb on + strand at 674.952 kb on + strand at 674.953 kb on - strand at 674.953 kb on - strand at 674.953 kb on - strand at 674.953 kb on - strand at 675.052 kb on - strand at 675.131 kb on - strand at 675.131 kb on - strand at 675.154 kb on - strand at 675.156 kb on - strand at 675.216 kb on + strand at 675.217 kb on - strand at 675.225 kb on + strand at 675.306 kb on + strand at 675.319 kb on + strand at 675.565 kb on + strand, within ABIE40_RS29920 at 675.565 kb on + strand, within ABIE40_RS29920 at 675.565 kb on + strand, within ABIE40_RS29920 at 675.565 kb on + strand, within ABIE40_RS29920 at 675.589 kb on + strand, within ABIE40_RS29920 at 675.589 kb on + strand, within ABIE40_RS29920 at 675.606 kb on + strand, within ABIE40_RS29920 at 675.606 kb on + strand, within ABIE40_RS29920 at 675.607 kb on - strand, within ABIE40_RS29920 at 675.642 kb on + strand, within ABIE40_RS29920 at 675.642 kb on + strand, within ABIE40_RS29920 at 675.642 kb on + strand, within ABIE40_RS29920 at 675.642 kb on + strand, within ABIE40_RS29920 at 675.642 kb on + strand, within ABIE40_RS29920 at 675.707 kb on + strand, within ABIE40_RS29920 at 675.707 kb on + strand, within ABIE40_RS29920 at 675.708 kb on - strand, within ABIE40_RS29920 at 675.865 kb on + strand, within ABIE40_RS29920 at 675.865 kb on + strand, within ABIE40_RS29920 at 676.065 kb on + strand at 676.065 kb on + strand at 676.066 kb on - strand at 676.066 kb on - strand at 676.209 kb on + strand, within ABIE40_RS29925 at 676.249 kb on + strand, within ABIE40_RS29925 at 676.250 kb on - strand, within ABIE40_RS29925 at 676.288 kb on - strand, within ABIE40_RS29925 at 676.397 kb on + strand, within ABIE40_RS29925 at 676.411 kb on + strand, within ABIE40_RS29925 at 676.412 kb on - strand, within ABIE40_RS29925 at 676.412 kb on - strand, within ABIE40_RS29925 at 676.412 kb on - strand, within ABIE40_RS29925 at 676.508 kb on - strand, within ABIE40_RS29925 at 676.661 kb on - strand, within ABIE40_RS29925 at 676.783 kb on + strand, within ABIE40_RS29925 at 676.783 kb on + strand, within ABIE40_RS29925 at 676.784 kb on - strand, within ABIE40_RS29925 at 676.803 kb on - strand, within ABIE40_RS29925 at 677.013 kb on + strand at 677.013 kb on + strand at 677.014 kb on - strand at 677.071 kb on + strand at 677.071 kb on + strand at 677.071 kb on + strand at 677.072 kb on - strand at 677.072 kb on - strand at 677.072 kb on - strand at 677.072 kb on - strand at 677.072 kb on - strand at 677.084 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with 6-Pentyl-2H-pyran-2-one 0.835572666666667 mM remove 674,404 - ABIE40_RS29910 0.84 +1.5 674,404 - ABIE40_RS29910 0.84 +1.5 674,404 - ABIE40_RS29910 0.84 -1.0 674,404 - ABIE40_RS29910 0.84 +2.5 674,559 + ABIE40_RS29915 0.29 +0.2 674,559 + ABIE40_RS29915 0.29 +1.0 674,592 + ABIE40_RS29915 0.38 +1.2 674,592 + ABIE40_RS29915 0.38 -1.8 674,592 + ABIE40_RS29915 0.38 -0.0 674,593 - ABIE40_RS29915 0.38 -0.8 674,593 - ABIE40_RS29915 0.38 -1.9 674,593 - ABIE40_RS29915 0.38 +1.5 674,692 + ABIE40_RS29915 0.66 -0.0 674,693 - ABIE40_RS29915 0.66 +1.3 674,952 + -2.8 674,952 + +0.2 674,953 - +2.5 674,953 - -0.6 674,953 - -1.0 674,953 - +0.1 675,052 - -0.6 675,131 - -1.7 675,131 - -1.3 675,154 - -0.7 675,156 - -0.5 675,216 + +2.0 675,217 - -1.0 675,225 + +0.2 675,306 + -0.3 675,319 + -0.6 675,565 + ABIE40_RS29920 0.32 +0.5 675,565 + ABIE40_RS29920 0.32 +0.6 675,565 + ABIE40_RS29920 0.32 +0.1 675,565 + ABIE40_RS29920 0.32 -0.8 675,589 + ABIE40_RS29920 0.35 +0.2 675,589 + ABIE40_RS29920 0.35 +1.1 675,606 + ABIE40_RS29920 0.37 +2.5 675,606 + ABIE40_RS29920 0.37 -0.3 675,607 - ABIE40_RS29920 0.37 -0.6 675,642 + ABIE40_RS29920 0.41 +0.5 675,642 + ABIE40_RS29920 0.41 -0.5 675,642 + ABIE40_RS29920 0.41 -0.6 675,642 + ABIE40_RS29920 0.41 +1.0 675,642 + ABIE40_RS29920 0.41 +0.6 675,707 + ABIE40_RS29920 0.49 +1.2 675,707 + ABIE40_RS29920 0.49 +0.7 675,708 - ABIE40_RS29920 0.50 +0.5 675,865 + ABIE40_RS29920 0.69 +0.1 675,865 + ABIE40_RS29920 0.69 +0.7 676,065 + -0.6 676,065 + -0.8 676,066 - -1.2 676,066 - -0.8 676,209 + ABIE40_RS29925 0.13 -1.2 676,249 + ABIE40_RS29925 0.17 +0.6 676,250 - ABIE40_RS29925 0.17 -2.1 676,288 - ABIE40_RS29925 0.21 +0.5 676,397 + ABIE40_RS29925 0.33 +1.3 676,411 + ABIE40_RS29925 0.35 +2.0 676,412 - ABIE40_RS29925 0.35 +1.5 676,412 - ABIE40_RS29925 0.35 +1.5 676,412 - ABIE40_RS29925 0.35 +2.5 676,508 - ABIE40_RS29925 0.45 -0.9 676,661 - ABIE40_RS29925 0.62 -0.0 676,783 + ABIE40_RS29925 0.75 +1.5 676,783 + ABIE40_RS29925 0.75 +2.0 676,784 - ABIE40_RS29925 0.75 +0.4 676,803 - ABIE40_RS29925 0.77 -1.0 677,013 + +1.0 677,013 + -1.3 677,014 - -0.3 677,071 + -1.8 677,071 + +0.3 677,071 + -1.0 677,072 - +2.0 677,072 - -0.3 677,072 - +0.7 677,072 - -1.3 677,072 - +1.1 677,084 - +1.5
Or see this region's nucleotide sequence