Experiment: R2A with 6-Pentyl-2H-pyran-2-one 0.835572666666667 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE40_RS10445 and ABIE40_RS10450 are separated by 213 nucleotides ABIE40_RS10450 and ABIE40_RS10455 are separated by 126 nucleotides
ABIE40_RS10445: ABIE40_RS10445 - UDP-2,3-diacylglucosamine diphosphatase, at 2,017,541 to 2,018,344
_RS10445
ABIE40_RS10450: ABIE40_RS10450 - NADP-dependent malic enzyme, at 2,018,558 to 2,020,870
_RS10450
ABIE40_RS10455: ABIE40_RS10455 - DUF2865 domain-containing protein, at 2,020,997 to 2,022,094
_RS10455
Position (kb)
2018
2019
2020
2021 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2017.636 kb on + strand, within ABIE40_RS10445 at 2017.695 kb on + strand, within ABIE40_RS10445 at 2017.696 kb on - strand, within ABIE40_RS10445 at 2017.696 kb on - strand, within ABIE40_RS10445 at 2017.735 kb on + strand, within ABIE40_RS10445 at 2017.857 kb on - strand, within ABIE40_RS10445 at 2017.923 kb on + strand, within ABIE40_RS10445 at 2017.923 kb on + strand, within ABIE40_RS10445 at 2017.924 kb on - strand, within ABIE40_RS10445 at 2017.960 kb on + strand, within ABIE40_RS10445 at 2017.961 kb on - strand, within ABIE40_RS10445 at 2018.002 kb on - strand, within ABIE40_RS10445 at 2018.002 kb on - strand, within ABIE40_RS10445 at 2018.109 kb on + strand, within ABIE40_RS10445 at 2018.109 kb on + strand, within ABIE40_RS10445 at 2018.109 kb on + strand, within ABIE40_RS10445 at 2018.109 kb on + strand, within ABIE40_RS10445 at 2018.109 kb on + strand, within ABIE40_RS10445 at 2018.109 kb on + strand, within ABIE40_RS10445 at 2018.109 kb on + strand, within ABIE40_RS10445 at 2018.110 kb on - strand, within ABIE40_RS10445 at 2018.110 kb on - strand, within ABIE40_RS10445 at 2018.110 kb on - strand, within ABIE40_RS10445 at 2018.110 kb on - strand, within ABIE40_RS10445 at 2018.110 kb on - strand, within ABIE40_RS10445 at 2018.110 kb on - strand, within ABIE40_RS10445 at 2018.110 kb on - strand, within ABIE40_RS10445 at 2018.168 kb on + strand, within ABIE40_RS10445 at 2018.168 kb on + strand, within ABIE40_RS10445 at 2018.169 kb on - strand, within ABIE40_RS10445 at 2018.169 kb on - strand, within ABIE40_RS10445 at 2018.182 kb on - strand, within ABIE40_RS10445 at 2018.237 kb on - strand, within ABIE40_RS10445 at 2018.237 kb on - strand, within ABIE40_RS10445 at 2018.250 kb on + strand, within ABIE40_RS10445 at 2018.251 kb on - strand, within ABIE40_RS10445 at 2018.338 kb on + strand at 2018.344 kb on + strand at 2018.344 kb on + strand at 2018.345 kb on - strand at 2018.345 kb on - strand at 2018.347 kb on + strand at 2018.348 kb on - strand at 2018.348 kb on - strand at 2018.348 kb on - strand at 2018.350 kb on - strand at 2018.512 kb on + strand at 2018.563 kb on + strand at 2018.774 kb on + strand at 2018.774 kb on + strand at 2018.775 kb on - strand at 2018.775 kb on - strand at 2018.776 kb on + strand at 2018.776 kb on + strand at 2018.776 kb on + strand at 2018.905 kb on + strand, within ABIE40_RS10450 at 2018.906 kb on - strand, within ABIE40_RS10450 at 2018.992 kb on + strand, within ABIE40_RS10450 at 2019.314 kb on + strand, within ABIE40_RS10450 at 2019.314 kb on + strand, within ABIE40_RS10450 at 2019.315 kb on - strand, within ABIE40_RS10450 at 2019.725 kb on + strand, within ABIE40_RS10450 at 2019.726 kb on - strand, within ABIE40_RS10450 at 2019.726 kb on - strand, within ABIE40_RS10450 at 2019.726 kb on - strand, within ABIE40_RS10450 at 2019.839 kb on + strand, within ABIE40_RS10450 at 2019.840 kb on - strand, within ABIE40_RS10450 at 2020.124 kb on + strand, within ABIE40_RS10450 at 2020.124 kb on + strand, within ABIE40_RS10450 at 2020.124 kb on + strand, within ABIE40_RS10450 at 2020.137 kb on - strand, within ABIE40_RS10450 at 2020.137 kb on - strand, within ABIE40_RS10450 at 2020.137 kb on - strand, within ABIE40_RS10450 at 2020.195 kb on + strand, within ABIE40_RS10450 at 2020.195 kb on + strand, within ABIE40_RS10450 at 2020.196 kb on - strand, within ABIE40_RS10450 at 2020.196 kb on - strand, within ABIE40_RS10450 at 2020.275 kb on - strand, within ABIE40_RS10450 at 2020.391 kb on + strand, within ABIE40_RS10450 at 2020.391 kb on + strand, within ABIE40_RS10450 at 2020.392 kb on - strand, within ABIE40_RS10450 at 2020.392 kb on - strand, within ABIE40_RS10450 at 2020.392 kb on - strand, within ABIE40_RS10450 at 2020.392 kb on - strand, within ABIE40_RS10450 at 2020.789 kb on + strand at 2020.789 kb on + strand at 2020.789 kb on + strand at 2020.790 kb on - strand at 2020.790 kb on - strand at 2020.897 kb on + strand at 2020.899 kb on + strand at 2020.934 kb on - strand at 2020.978 kb on + strand at 2020.978 kb on + strand at 2020.978 kb on + strand at 2020.979 kb on - strand at 2020.979 kb on - strand at 2021.141 kb on + strand, within ABIE40_RS10455 at 2021.141 kb on + strand, within ABIE40_RS10455 at 2021.142 kb on - strand, within ABIE40_RS10455 at 2021.142 kb on - strand, within ABIE40_RS10455 at 2021.142 kb on - strand, within ABIE40_RS10455 at 2021.306 kb on + strand, within ABIE40_RS10455 at 2021.306 kb on + strand, within ABIE40_RS10455 at 2021.306 kb on + strand, within ABIE40_RS10455 at 2021.307 kb on - strand, within ABIE40_RS10455 at 2021.483 kb on - strand, within ABIE40_RS10455 at 2021.483 kb on - strand, within ABIE40_RS10455 at 2021.483 kb on - strand, within ABIE40_RS10455 at 2021.525 kb on + strand, within ABIE40_RS10455 at 2021.525 kb on + strand, within ABIE40_RS10455 at 2021.526 kb on - strand, within ABIE40_RS10455 at 2021.690 kb on + strand, within ABIE40_RS10455 at 2021.690 kb on + strand, within ABIE40_RS10455 at 2021.691 kb on - strand, within ABIE40_RS10455 at 2021.722 kb on + strand, within ABIE40_RS10455 at 2021.722 kb on + strand, within ABIE40_RS10455 at 2021.722 kb on + strand, within ABIE40_RS10455 at 2021.723 kb on - strand, within ABIE40_RS10455 at 2021.723 kb on - strand, within ABIE40_RS10455 at 2021.723 kb on - strand, within ABIE40_RS10455 at 2021.723 kb on - strand, within ABIE40_RS10455 at 2021.723 kb on - strand, within ABIE40_RS10455 at 2021.723 kb on - strand, within ABIE40_RS10455 at 2021.778 kb on - strand, within ABIE40_RS10455 at 2021.831 kb on + strand, within ABIE40_RS10455 at 2021.832 kb on - strand, within ABIE40_RS10455 at 2021.832 kb on - strand, within ABIE40_RS10455 at 2021.834 kb on + strand, within ABIE40_RS10455 at 2021.834 kb on + strand, within ABIE40_RS10455 at 2021.835 kb on - strand, within ABIE40_RS10455 at 2021.835 kb on - strand, within ABIE40_RS10455
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with 6-Pentyl-2H-pyran-2-one 0.835572666666667 mM remove 2,017,636 + ABIE40_RS10445 0.12 -1.6 2,017,695 + ABIE40_RS10445 0.19 +1.1 2,017,696 - ABIE40_RS10445 0.19 +0.1 2,017,696 - ABIE40_RS10445 0.19 -0.4 2,017,735 + ABIE40_RS10445 0.24 -0.4 2,017,857 - ABIE40_RS10445 0.39 +1.9 2,017,923 + ABIE40_RS10445 0.48 +1.2 2,017,923 + ABIE40_RS10445 0.48 -0.6 2,017,924 - ABIE40_RS10445 0.48 +0.9 2,017,960 + ABIE40_RS10445 0.52 +0.6 2,017,961 - ABIE40_RS10445 0.52 -0.7 2,018,002 - ABIE40_RS10445 0.57 +0.9 2,018,002 - ABIE40_RS10445 0.57 +1.1 2,018,109 + ABIE40_RS10445 0.71 -0.8 2,018,109 + ABIE40_RS10445 0.71 -1.0 2,018,109 + ABIE40_RS10445 0.71 -0.8 2,018,109 + ABIE40_RS10445 0.71 -0.1 2,018,109 + ABIE40_RS10445 0.71 -0.9 2,018,109 + ABIE40_RS10445 0.71 +2.0 2,018,109 + ABIE40_RS10445 0.71 +0.9 2,018,110 - ABIE40_RS10445 0.71 -0.8 2,018,110 - ABIE40_RS10445 0.71 +0.6 2,018,110 - ABIE40_RS10445 0.71 +0.8 2,018,110 - ABIE40_RS10445 0.71 +0.5 2,018,110 - ABIE40_RS10445 0.71 +0.4 2,018,110 - ABIE40_RS10445 0.71 -0.2 2,018,110 - ABIE40_RS10445 0.71 -0.9 2,018,168 + ABIE40_RS10445 0.78 -1.7 2,018,168 + ABIE40_RS10445 0.78 +0.5 2,018,169 - ABIE40_RS10445 0.78 +0.7 2,018,169 - ABIE40_RS10445 0.78 -2.0 2,018,182 - ABIE40_RS10445 0.80 -0.9 2,018,237 - ABIE40_RS10445 0.87 -0.5 2,018,237 - ABIE40_RS10445 0.87 -0.7 2,018,250 + ABIE40_RS10445 0.88 +0.5 2,018,251 - ABIE40_RS10445 0.88 -0.9 2,018,338 + +0.4 2,018,344 + -3.2 2,018,344 + +1.2 2,018,345 - -0.3 2,018,345 - +0.4 2,018,347 + -0.6 2,018,348 - +1.5 2,018,348 - -0.1 2,018,348 - -1.6 2,018,350 - -1.2 2,018,512 + -0.6 2,018,563 + -0.9 2,018,774 + -2.0 2,018,774 + +0.2 2,018,775 - +2.5 2,018,775 - +2.5 2,018,776 + +0.2 2,018,776 + +0.5 2,018,776 + -1.3 2,018,905 + ABIE40_RS10450 0.15 -1.4 2,018,906 - ABIE40_RS10450 0.15 -1.7 2,018,992 + ABIE40_RS10450 0.19 +0.2 2,019,314 + ABIE40_RS10450 0.33 +0.9 2,019,314 + ABIE40_RS10450 0.33 -1.1 2,019,315 - ABIE40_RS10450 0.33 -0.3 2,019,725 + ABIE40_RS10450 0.50 -1.4 2,019,726 - ABIE40_RS10450 0.50 -1.4 2,019,726 - ABIE40_RS10450 0.50 -2.1 2,019,726 - ABIE40_RS10450 0.50 -1.2 2,019,839 + ABIE40_RS10450 0.55 -2.2 2,019,840 - ABIE40_RS10450 0.55 -0.8 2,020,124 + ABIE40_RS10450 0.68 -0.8 2,020,124 + ABIE40_RS10450 0.68 -2.1 2,020,124 + ABIE40_RS10450 0.68 -2.5 2,020,137 - ABIE40_RS10450 0.68 -1.4 2,020,137 - ABIE40_RS10450 0.68 -0.3 2,020,137 - ABIE40_RS10450 0.68 -0.1 2,020,195 + ABIE40_RS10450 0.71 -0.2 2,020,195 + ABIE40_RS10450 0.71 -1.0 2,020,196 - ABIE40_RS10450 0.71 +0.9 2,020,196 - ABIE40_RS10450 0.71 -1.9 2,020,275 - ABIE40_RS10450 0.74 -0.8 2,020,391 + ABIE40_RS10450 0.79 +0.5 2,020,391 + ABIE40_RS10450 0.79 -1.7 2,020,392 - ABIE40_RS10450 0.79 -0.1 2,020,392 - ABIE40_RS10450 0.79 -1.6 2,020,392 - ABIE40_RS10450 0.79 -1.3 2,020,392 - ABIE40_RS10450 0.79 -0.9 2,020,789 + -0.3 2,020,789 + -1.4 2,020,789 + +0.0 2,020,790 - -0.7 2,020,790 - -0.7 2,020,897 + -0.8 2,020,899 + +0.8 2,020,934 - +0.7 2,020,978 + +0.5 2,020,978 + -0.1 2,020,978 + -0.0 2,020,979 - +0.5 2,020,979 - +0.7 2,021,141 + ABIE40_RS10455 0.13 -0.8 2,021,141 + ABIE40_RS10455 0.13 -0.4 2,021,142 - ABIE40_RS10455 0.13 -1.7 2,021,142 - ABIE40_RS10455 0.13 +0.1 2,021,142 - ABIE40_RS10455 0.13 +0.3 2,021,306 + ABIE40_RS10455 0.28 -1.7 2,021,306 + ABIE40_RS10455 0.28 -0.4 2,021,306 + ABIE40_RS10455 0.28 +0.9 2,021,307 - ABIE40_RS10455 0.28 -0.4 2,021,483 - ABIE40_RS10455 0.44 -1.3 2,021,483 - ABIE40_RS10455 0.44 -0.0 2,021,483 - ABIE40_RS10455 0.44 +1.2 2,021,525 + ABIE40_RS10455 0.48 -0.2 2,021,525 + ABIE40_RS10455 0.48 -0.5 2,021,526 - ABIE40_RS10455 0.48 +0.9 2,021,690 + ABIE40_RS10455 0.63 +1.1 2,021,690 + ABIE40_RS10455 0.63 +0.2 2,021,691 - ABIE40_RS10455 0.63 -1.2 2,021,722 + ABIE40_RS10455 0.66 +0.2 2,021,722 + ABIE40_RS10455 0.66 -1.2 2,021,722 + ABIE40_RS10455 0.66 -1.2 2,021,723 - ABIE40_RS10455 0.66 -0.7 2,021,723 - ABIE40_RS10455 0.66 -0.3 2,021,723 - ABIE40_RS10455 0.66 -0.1 2,021,723 - ABIE40_RS10455 0.66 +0.6 2,021,723 - ABIE40_RS10455 0.66 +0.1 2,021,723 - ABIE40_RS10455 0.66 +1.5 2,021,778 - ABIE40_RS10455 0.71 +1.0 2,021,831 + ABIE40_RS10455 0.76 +0.1 2,021,832 - ABIE40_RS10455 0.76 +0.0 2,021,832 - ABIE40_RS10455 0.76 +0.0 2,021,834 + ABIE40_RS10455 0.76 -0.2 2,021,834 + ABIE40_RS10455 0.76 +1.1 2,021,835 - ABIE40_RS10455 0.76 -0.4 2,021,835 - ABIE40_RS10455 0.76 +1.2
Or see this region's nucleotide sequence