Experiment: R2A with 6-Pentyl-2H-pyran-2-one 0.835572666666667 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE40_RS10300 and ABIE40_RS10305 are separated by 126 nucleotides ABIE40_RS10305 and ABIE40_RS10310 are separated by 17 nucleotides ABIE40_RS10310 and ABIE40_RS10315 are separated by 32 nucleotides
ABIE40_RS10300: ABIE40_RS10300 - AraC family transcriptional regulator, at 1,981,523 to 1,982,374
_RS10300
ABIE40_RS10305: ABIE40_RS10305 - LysE family translocator, at 1,982,501 to 1,983,133
_RS10305
ABIE40_RS10310: ABIE40_RS10310 - STM3941 family protein, at 1,983,151 to 1,983,651
_RS10310
ABIE40_RS10315: ABIE40_RS10315 - alanine--tRNA ligase, at 1,983,684 to 1,986,344
_RS10315
Position (kb)
1983
1984 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1982.229 kb on + strand, within ABIE40_RS10300 at 1982.230 kb on - strand, within ABIE40_RS10300 at 1982.247 kb on + strand, within ABIE40_RS10300 at 1982.247 kb on + strand, within ABIE40_RS10300 at 1982.248 kb on - strand, within ABIE40_RS10300 at 1982.248 kb on - strand, within ABIE40_RS10300 at 1982.310 kb on + strand at 1982.310 kb on + strand at 1982.311 kb on - strand at 1982.311 kb on - strand at 1982.311 kb on - strand at 1982.311 kb on - strand at 1982.311 kb on - strand at 1982.311 kb on - strand at 1982.311 kb on - strand at 1982.311 kb on - strand at 1982.311 kb on - strand at 1982.314 kb on + strand at 1982.314 kb on + strand at 1982.315 kb on - strand at 1982.315 kb on - strand at 1982.315 kb on - strand at 1982.316 kb on + strand at 1982.316 kb on + strand at 1982.316 kb on + strand at 1982.316 kb on + strand at 1982.317 kb on - strand at 1982.317 kb on - strand at 1982.317 kb on - strand at 1982.414 kb on - strand at 1982.462 kb on + strand at 1982.463 kb on - strand at 1982.499 kb on + strand at 1982.630 kb on + strand, within ABIE40_RS10305 at 1982.630 kb on + strand, within ABIE40_RS10305 at 1982.630 kb on + strand, within ABIE40_RS10305 at 1982.631 kb on - strand, within ABIE40_RS10305 at 1982.631 kb on - strand, within ABIE40_RS10305 at 1982.631 kb on - strand, within ABIE40_RS10305 at 1982.655 kb on - strand, within ABIE40_RS10305 at 1982.655 kb on - strand, within ABIE40_RS10305 at 1982.718 kb on - strand, within ABIE40_RS10305 at 1982.748 kb on - strand, within ABIE40_RS10305 at 1982.748 kb on - strand, within ABIE40_RS10305 at 1982.757 kb on - strand, within ABIE40_RS10305 at 1982.757 kb on - strand, within ABIE40_RS10305 at 1982.757 kb on - strand, within ABIE40_RS10305 at 1982.765 kb on + strand, within ABIE40_RS10305 at 1982.766 kb on - strand, within ABIE40_RS10305 at 1982.766 kb on - strand, within ABIE40_RS10305 at 1982.766 kb on - strand, within ABIE40_RS10305 at 1982.955 kb on + strand, within ABIE40_RS10305 at 1982.955 kb on + strand, within ABIE40_RS10305 at 1982.955 kb on + strand, within ABIE40_RS10305 at 1982.955 kb on + strand, within ABIE40_RS10305 at 1982.955 kb on + strand, within ABIE40_RS10305 at 1982.956 kb on - strand, within ABIE40_RS10305 at 1982.956 kb on - strand, within ABIE40_RS10305 at 1982.956 kb on - strand, within ABIE40_RS10305 at 1983.024 kb on + strand, within ABIE40_RS10305 at 1983.024 kb on + strand, within ABIE40_RS10305 at 1983.024 kb on + strand, within ABIE40_RS10305 at 1983.025 kb on - strand, within ABIE40_RS10305 at 1983.025 kb on - strand, within ABIE40_RS10305 at 1983.072 kb on - strand at 1983.242 kb on + strand, within ABIE40_RS10310 at 1983.242 kb on + strand, within ABIE40_RS10310 at 1983.242 kb on + strand, within ABIE40_RS10310 at 1983.243 kb on - strand, within ABIE40_RS10310 at 1983.243 kb on - strand, within ABIE40_RS10310 at 1983.482 kb on - strand, within ABIE40_RS10310 at 1983.482 kb on - strand, within ABIE40_RS10310
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with 6-Pentyl-2H-pyran-2-one 0.835572666666667 mM remove 1,982,229 + ABIE40_RS10300 0.83 -0.8 1,982,230 - ABIE40_RS10300 0.83 -0.8 1,982,247 + ABIE40_RS10300 0.85 -0.5 1,982,247 + ABIE40_RS10300 0.85 +0.3 1,982,248 - ABIE40_RS10300 0.85 +1.2 1,982,248 - ABIE40_RS10300 0.85 +0.8 1,982,310 + +0.0 1,982,310 + -0.3 1,982,311 - -1.6 1,982,311 - -0.4 1,982,311 - -0.2 1,982,311 - +1.1 1,982,311 - +0.4 1,982,311 - -0.5 1,982,311 - +0.1 1,982,311 - +1.2 1,982,311 - -1.0 1,982,314 + +0.3 1,982,314 + -0.9 1,982,315 - +0.2 1,982,315 - -0.5 1,982,315 - -0.4 1,982,316 + -0.0 1,982,316 + -1.2 1,982,316 + -0.7 1,982,316 + -1.7 1,982,317 - +0.1 1,982,317 - -1.2 1,982,317 - +1.0 1,982,414 - +0.2 1,982,462 + +0.1 1,982,463 - +0.9 1,982,499 + +0.0 1,982,630 + ABIE40_RS10305 0.20 -0.9 1,982,630 + ABIE40_RS10305 0.20 +2.5 1,982,630 + ABIE40_RS10305 0.20 +0.2 1,982,631 - ABIE40_RS10305 0.21 -0.2 1,982,631 - ABIE40_RS10305 0.21 -0.1 1,982,631 - ABIE40_RS10305 0.21 -1.6 1,982,655 - ABIE40_RS10305 0.24 -0.7 1,982,655 - ABIE40_RS10305 0.24 +0.1 1,982,718 - ABIE40_RS10305 0.34 -0.7 1,982,748 - ABIE40_RS10305 0.39 -0.2 1,982,748 - ABIE40_RS10305 0.39 +1.5 1,982,757 - ABIE40_RS10305 0.40 -0.7 1,982,757 - ABIE40_RS10305 0.40 -0.5 1,982,757 - ABIE40_RS10305 0.40 -1.2 1,982,765 + ABIE40_RS10305 0.42 +2.5 1,982,766 - ABIE40_RS10305 0.42 -0.3 1,982,766 - ABIE40_RS10305 0.42 +0.5 1,982,766 - ABIE40_RS10305 0.42 +0.5 1,982,955 + ABIE40_RS10305 0.72 +0.1 1,982,955 + ABIE40_RS10305 0.72 +0.2 1,982,955 + ABIE40_RS10305 0.72 +0.9 1,982,955 + ABIE40_RS10305 0.72 +0.1 1,982,955 + ABIE40_RS10305 0.72 +1.1 1,982,956 - ABIE40_RS10305 0.72 -0.6 1,982,956 - ABIE40_RS10305 0.72 -0.1 1,982,956 - ABIE40_RS10305 0.72 -0.4 1,983,024 + ABIE40_RS10305 0.83 +1.1 1,983,024 + ABIE40_RS10305 0.83 +1.3 1,983,024 + ABIE40_RS10305 0.83 +0.4 1,983,025 - ABIE40_RS10305 0.83 -2.5 1,983,025 - ABIE40_RS10305 0.83 -1.1 1,983,072 - -2.3 1,983,242 + ABIE40_RS10310 0.18 -0.7 1,983,242 + ABIE40_RS10310 0.18 +0.9 1,983,242 + ABIE40_RS10310 0.18 +0.5 1,983,243 - ABIE40_RS10310 0.18 +2.0 1,983,243 - ABIE40_RS10310 0.18 +1.5 1,983,482 - ABIE40_RS10310 0.66 +0.0 1,983,482 - ABIE40_RS10310 0.66 +0.2
Or see this region's nucleotide sequence