Experiment: R2A with 6-Pentyl-2H-pyran-2-one 0.835572666666667 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE40_RS09705 and ABIE40_RS09710 overlap by 4 nucleotides ABIE40_RS09710 and ABIE40_RS09715 are separated by 4 nucleotides ABIE40_RS09715 and ABIE40_RS09720 are separated by 301 nucleotides ABIE40_RS09720 and ABIE40_RS09725 are separated by 26 nucleotides
ABIE40_RS09705: ABIE40_RS09705 - DUF1697 domain-containing protein, at 1,874,871 to 1,875,395
_RS09705
ABIE40_RS09710: ABIE40_RS09710 - membrane protein insertion efficiency factor YidD, at 1,875,392 to 1,875,787
_RS09710
ABIE40_RS09715: ABIE40_RS09715 - iron-sulfur cluster assembly scaffold protein, at 1,875,792 to 1,876,238
_RS09715
ABIE40_RS09720: ABIE40_RS09720 - GTP cyclohydrolase I FolE, at 1,876,540 to 1,877,154
_RS09720
ABIE40_RS09725: ABIE40_RS09725 - phosphoribosyl-AMP cyclohydrolase, at 1,877,181 to 1,877,633
_RS09725
Position (kb)
1875
1876
1877 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1874.833 kb on - strand at 1874.851 kb on - strand at 1874.851 kb on - strand at 1874.872 kb on + strand at 1874.873 kb on - strand at 1874.873 kb on - strand at 1874.876 kb on + strand at 1874.876 kb on + strand at 1874.876 kb on + strand at 1874.876 kb on + strand at 1874.877 kb on - strand at 1874.877 kb on - strand at 1875.037 kb on + strand, within ABIE40_RS09705 at 1875.038 kb on - strand, within ABIE40_RS09705 at 1875.173 kb on + strand, within ABIE40_RS09705 at 1875.174 kb on - strand, within ABIE40_RS09705 at 1875.174 kb on - strand, within ABIE40_RS09705 at 1875.174 kb on - strand, within ABIE40_RS09705 at 1875.260 kb on + strand, within ABIE40_RS09705 at 1875.260 kb on + strand, within ABIE40_RS09705 at 1875.261 kb on - strand, within ABIE40_RS09705 at 1875.261 kb on - strand, within ABIE40_RS09705 at 1875.261 kb on - strand, within ABIE40_RS09705 at 1875.261 kb on - strand, within ABIE40_RS09705 at 1875.261 kb on - strand, within ABIE40_RS09705 at 1875.263 kb on + strand, within ABIE40_RS09705 at 1875.263 kb on + strand, within ABIE40_RS09705 at 1875.263 kb on + strand, within ABIE40_RS09705 at 1875.264 kb on - strand, within ABIE40_RS09705 at 1875.264 kb on - strand, within ABIE40_RS09705 at 1875.385 kb on + strand at 1875.385 kb on + strand at 1875.385 kb on + strand at 1875.386 kb on - strand at 1875.386 kb on - strand at 1875.386 kb on - strand at 1875.386 kb on - strand at 1875.386 kb on - strand at 1875.421 kb on - strand at 1875.567 kb on + strand, within ABIE40_RS09710 at 1875.568 kb on - strand, within ABIE40_RS09710 at 1875.592 kb on + strand, within ABIE40_RS09710 at 1875.592 kb on + strand, within ABIE40_RS09710 at 1875.592 kb on + strand, within ABIE40_RS09710 at 1875.592 kb on + strand, within ABIE40_RS09710 at 1875.593 kb on - strand, within ABIE40_RS09710 at 1875.593 kb on - strand, within ABIE40_RS09710 at 1875.697 kb on - strand, within ABIE40_RS09710 at 1875.889 kb on + strand, within ABIE40_RS09715 at 1875.889 kb on + strand, within ABIE40_RS09715 at 1875.889 kb on + strand, within ABIE40_RS09715 at 1875.889 kb on + strand, within ABIE40_RS09715 at 1875.890 kb on - strand, within ABIE40_RS09715 at 1875.890 kb on - strand, within ABIE40_RS09715 at 1875.910 kb on + strand, within ABIE40_RS09715 at 1875.910 kb on + strand, within ABIE40_RS09715 at 1876.193 kb on + strand, within ABIE40_RS09715 at 1876.194 kb on - strand at 1876.225 kb on + strand at 1876.226 kb on - strand at 1876.289 kb on + strand at 1876.289 kb on + strand at 1876.289 kb on + strand at 1876.290 kb on - strand at 1876.290 kb on - strand at 1876.290 kb on - strand at 1876.294 kb on + strand at 1876.295 kb on - strand at 1876.325 kb on + strand at 1876.325 kb on + strand at 1876.326 kb on - strand at 1876.329 kb on + strand at 1876.329 kb on + strand at 1876.329 kb on + strand at 1876.329 kb on + strand at 1876.329 kb on + strand at 1876.330 kb on - strand at 1876.330 kb on - strand at 1876.330 kb on - strand at 1876.351 kb on - strand at 1876.351 kb on - strand at 1876.351 kb on - strand at 1876.352 kb on + strand at 1876.352 kb on + strand at 1876.352 kb on + strand at 1876.352 kb on + strand at 1876.352 kb on + strand at 1876.352 kb on + strand at 1876.352 kb on + strand at 1876.352 kb on + strand at 1876.352 kb on + strand at 1876.353 kb on - strand at 1876.353 kb on - strand at 1876.353 kb on - strand at 1876.353 kb on - strand at 1876.353 kb on - strand at 1876.353 kb on - strand at 1876.353 kb on - strand at 1876.356 kb on + strand at 1876.356 kb on + strand at 1876.356 kb on + strand at 1876.356 kb on + strand at 1876.356 kb on + strand at 1876.356 kb on + strand at 1876.356 kb on + strand at 1876.356 kb on + strand at 1876.356 kb on + strand at 1876.356 kb on + strand at 1876.356 kb on + strand at 1876.356 kb on + strand at 1876.356 kb on + strand at 1876.357 kb on - strand at 1876.357 kb on - strand at 1876.357 kb on - strand at 1876.482 kb on - strand at 1876.539 kb on + strand at 1876.540 kb on - strand at 1876.540 kb on - strand at 1876.554 kb on - strand at 1876.721 kb on - strand, within ABIE40_RS09720 at 1876.774 kb on + strand, within ABIE40_RS09720 at 1876.864 kb on + strand, within ABIE40_RS09720 at 1876.964 kb on + strand, within ABIE40_RS09720 at 1877.028 kb on + strand, within ABIE40_RS09720 at 1877.028 kb on + strand, within ABIE40_RS09720 at 1877.028 kb on + strand, within ABIE40_RS09720 at 1877.029 kb on - strand, within ABIE40_RS09720 at 1877.029 kb on - strand, within ABIE40_RS09720 at 1877.029 kb on - strand, within ABIE40_RS09720 at 1877.029 kb on - strand, within ABIE40_RS09720 at 1877.029 kb on - strand, within ABIE40_RS09720 at 1877.029 kb on - strand, within ABIE40_RS09720 at 1877.088 kb on + strand, within ABIE40_RS09720 at 1877.166 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with 6-Pentyl-2H-pyran-2-one 0.835572666666667 mM remove 1,874,833 - -2.5 1,874,851 - -1.9 1,874,851 - +0.6 1,874,872 + +1.4 1,874,873 - -0.8 1,874,873 - -2.0 1,874,876 + -0.5 1,874,876 + +1.4 1,874,876 + -0.2 1,874,876 + -0.3 1,874,877 - +0.8 1,874,877 - -0.4 1,875,037 + ABIE40_RS09705 0.32 -1.1 1,875,038 - ABIE40_RS09705 0.32 +0.4 1,875,173 + ABIE40_RS09705 0.58 +0.8 1,875,174 - ABIE40_RS09705 0.58 -0.9 1,875,174 - ABIE40_RS09705 0.58 -0.7 1,875,174 - ABIE40_RS09705 0.58 -1.5 1,875,260 + ABIE40_RS09705 0.74 +0.8 1,875,260 + ABIE40_RS09705 0.74 +1.4 1,875,261 - ABIE40_RS09705 0.74 -1.5 1,875,261 - ABIE40_RS09705 0.74 -1.6 1,875,261 - ABIE40_RS09705 0.74 -0.9 1,875,261 - ABIE40_RS09705 0.74 -0.3 1,875,261 - ABIE40_RS09705 0.74 +0.6 1,875,263 + ABIE40_RS09705 0.75 -2.2 1,875,263 + ABIE40_RS09705 0.75 -1.3 1,875,263 + ABIE40_RS09705 0.75 -0.1 1,875,264 - ABIE40_RS09705 0.75 -0.8 1,875,264 - ABIE40_RS09705 0.75 -1.5 1,875,385 + -1.7 1,875,385 + -0.4 1,875,385 + +0.0 1,875,386 - +0.6 1,875,386 - -0.9 1,875,386 - -1.2 1,875,386 - -0.7 1,875,386 - +0.9 1,875,421 - +0.1 1,875,567 + ABIE40_RS09710 0.44 -1.0 1,875,568 - ABIE40_RS09710 0.44 -0.2 1,875,592 + ABIE40_RS09710 0.51 -0.4 1,875,592 + ABIE40_RS09710 0.51 +1.4 1,875,592 + ABIE40_RS09710 0.51 -1.9 1,875,592 + ABIE40_RS09710 0.51 +1.4 1,875,593 - ABIE40_RS09710 0.51 +0.4 1,875,593 - ABIE40_RS09710 0.51 -1.7 1,875,697 - ABIE40_RS09710 0.77 +1.8 1,875,889 + ABIE40_RS09715 0.22 -0.4 1,875,889 + ABIE40_RS09715 0.22 -2.0 1,875,889 + ABIE40_RS09715 0.22 -0.7 1,875,889 + ABIE40_RS09715 0.22 -0.4 1,875,890 - ABIE40_RS09715 0.22 -0.3 1,875,890 - ABIE40_RS09715 0.22 -1.5 1,875,910 + ABIE40_RS09715 0.26 -1.4 1,875,910 + ABIE40_RS09715 0.26 +2.4 1,876,193 + ABIE40_RS09715 0.90 -1.1 1,876,194 - +0.3 1,876,225 + +2.4 1,876,226 - +1.2 1,876,289 + -0.7 1,876,289 + +0.3 1,876,289 + +0.2 1,876,290 - -0.1 1,876,290 - -0.1 1,876,290 - -2.2 1,876,294 + +0.6 1,876,295 - +0.7 1,876,325 + +1.9 1,876,325 + +0.1 1,876,326 - -0.2 1,876,329 + +0.2 1,876,329 + -0.7 1,876,329 + -1.3 1,876,329 + +0.1 1,876,329 + +1.2 1,876,330 - -0.4 1,876,330 - -0.6 1,876,330 - -2.4 1,876,351 - -1.6 1,876,351 - -1.1 1,876,351 - +0.1 1,876,352 + -0.3 1,876,352 + +0.8 1,876,352 + +0.1 1,876,352 + -1.7 1,876,352 + -0.8 1,876,352 + -0.5 1,876,352 + -0.9 1,876,352 + -0.5 1,876,352 + +0.2 1,876,353 - -0.7 1,876,353 - -0.1 1,876,353 - +1.6 1,876,353 - -2.4 1,876,353 - -0.5 1,876,353 - +0.8 1,876,353 - +0.0 1,876,356 + +0.7 1,876,356 + +0.1 1,876,356 + -0.3 1,876,356 + -0.4 1,876,356 + -0.3 1,876,356 + -0.4 1,876,356 + +0.1 1,876,356 + -0.4 1,876,356 + +0.7 1,876,356 + -0.5 1,876,356 + -0.7 1,876,356 + -0.8 1,876,356 + +0.5 1,876,357 - +2.0 1,876,357 - -1.4 1,876,357 - -1.8 1,876,482 - +0.2 1,876,539 + -0.5 1,876,540 - +1.8 1,876,540 - +1.0 1,876,554 - +0.3 1,876,721 - ABIE40_RS09720 0.29 +1.6 1,876,774 + ABIE40_RS09720 0.38 +1.4 1,876,864 + ABIE40_RS09720 0.53 -0.6 1,876,964 + ABIE40_RS09720 0.69 +0.6 1,877,028 + ABIE40_RS09720 0.79 -1.7 1,877,028 + ABIE40_RS09720 0.79 +2.0 1,877,028 + ABIE40_RS09720 0.79 +2.4 1,877,029 - ABIE40_RS09720 0.80 +0.8 1,877,029 - ABIE40_RS09720 0.80 -0.7 1,877,029 - ABIE40_RS09720 0.80 +0.1 1,877,029 - ABIE40_RS09720 0.80 +0.4 1,877,029 - ABIE40_RS09720 0.80 +1.9 1,877,029 - ABIE40_RS09720 0.80 +2.4 1,877,088 + ABIE40_RS09720 0.89 -0.9 1,877,166 - -0.1
Or see this region's nucleotide sequence