Experiment: R2A with 6-Pentyl-2H-pyran-2-one 0.835572666666667 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE40_RS20490 and ABIE40_RS20495 are separated by 5 nucleotides ABIE40_RS20495 and ABIE40_RS20500 are separated by 12 nucleotides ABIE40_RS20500 and ABIE40_RS20505 are separated by 115 nucleotides ABIE40_RS20505 and ABIE40_RS20510 are separated by 138 nucleotides
ABIE40_RS20490: ABIE40_RS20490 - cytochrome ubiquinol oxidase subunit I, at 202,225 to 203,805
_RS20490
ABIE40_RS20495: ABIE40_RS20495 - cytochrome d ubiquinol oxidase subunit II, at 203,811 to 204,962
_RS20495
ABIE40_RS20500: ABIE40_RS20500 - cytochrome bd-I oxidase subunit CydX, at 204,975 to 205,085
_RS20500
ABIE40_RS20505: ABIE40_RS20505 - hypothetical protein, at 205,201 to 205,503
_RS20505
ABIE40_RS20510: ABIE40_RS20510 - TerC family protein, at 205,642 to 206,259
_RS20510
Position (kb)
203
204
205 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 202.844 kb on - strand, within ABIE40_RS20490 at 202.844 kb on - strand, within ABIE40_RS20490 at 202.844 kb on - strand, within ABIE40_RS20490 at 202.844 kb on - strand, within ABIE40_RS20490 at 202.955 kb on - strand, within ABIE40_RS20490 at 203.089 kb on - strand, within ABIE40_RS20490 at 203.089 kb on - strand, within ABIE40_RS20490 at 203.089 kb on - strand, within ABIE40_RS20490 at 203.200 kb on + strand, within ABIE40_RS20490 at 203.200 kb on + strand, within ABIE40_RS20490 at 203.200 kb on + strand, within ABIE40_RS20490 at 203.201 kb on - strand, within ABIE40_RS20490 at 203.201 kb on - strand, within ABIE40_RS20490 at 203.201 kb on - strand, within ABIE40_RS20490 at 203.296 kb on + strand, within ABIE40_RS20490 at 203.317 kb on + strand, within ABIE40_RS20490 at 203.317 kb on + strand, within ABIE40_RS20490 at 203.318 kb on - strand, within ABIE40_RS20490 at 203.318 kb on - strand, within ABIE40_RS20490 at 203.318 kb on - strand, within ABIE40_RS20490 at 203.494 kb on + strand, within ABIE40_RS20490 at 203.495 kb on - strand, within ABIE40_RS20490 at 203.495 kb on - strand, within ABIE40_RS20490 at 203.683 kb on + strand at 203.683 kb on + strand at 203.683 kb on + strand at 203.683 kb on + strand at 203.683 kb on + strand at 203.683 kb on + strand at 203.684 kb on - strand at 203.684 kb on - strand at 203.685 kb on + strand at 203.686 kb on - strand at 203.686 kb on - strand at 203.835 kb on + strand at 203.835 kb on + strand at 203.840 kb on + strand at 203.841 kb on - strand at 203.841 kb on - strand at 203.924 kb on + strand at 203.924 kb on + strand at 203.990 kb on + strand, within ABIE40_RS20495 at 204.060 kb on + strand, within ABIE40_RS20495 at 204.084 kb on + strand, within ABIE40_RS20495 at 204.084 kb on + strand, within ABIE40_RS20495 at 204.085 kb on - strand, within ABIE40_RS20495 at 204.141 kb on + strand, within ABIE40_RS20495 at 204.142 kb on - strand, within ABIE40_RS20495 at 204.158 kb on + strand, within ABIE40_RS20495 at 204.295 kb on - strand, within ABIE40_RS20495 at 204.327 kb on + strand, within ABIE40_RS20495 at 204.328 kb on - strand, within ABIE40_RS20495 at 204.432 kb on + strand, within ABIE40_RS20495 at 204.432 kb on + strand, within ABIE40_RS20495 at 204.515 kb on + strand, within ABIE40_RS20495 at 204.516 kb on - strand, within ABIE40_RS20495 at 204.516 kb on - strand, within ABIE40_RS20495 at 204.516 kb on - strand, within ABIE40_RS20495 at 204.516 kb on - strand, within ABIE40_RS20495 at 204.516 kb on - strand, within ABIE40_RS20495 at 204.516 kb on - strand, within ABIE40_RS20495 at 204.597 kb on + strand, within ABIE40_RS20495 at 204.598 kb on - strand, within ABIE40_RS20495 at 204.598 kb on - strand, within ABIE40_RS20495 at 204.606 kb on + strand, within ABIE40_RS20495 at 204.607 kb on - strand, within ABIE40_RS20495 at 204.607 kb on - strand, within ABIE40_RS20495 at 204.607 kb on - strand, within ABIE40_RS20495 at 204.607 kb on - strand, within ABIE40_RS20495 at 204.734 kb on + strand, within ABIE40_RS20495 at 204.735 kb on - strand, within ABIE40_RS20495 at 204.824 kb on + strand, within ABIE40_RS20495 at 204.824 kb on + strand, within ABIE40_RS20495 at 204.898 kb on - strand at 204.898 kb on - strand at 204.957 kb on + strand at 204.960 kb on + strand at 204.960 kb on + strand at 204.960 kb on + strand at 204.982 kb on - strand at 205.044 kb on + strand, within ABIE40_RS20500 at 205.045 kb on - strand, within ABIE40_RS20500 at 205.159 kb on - strand at 205.159 kb on - strand at 205.161 kb on + strand at 205.161 kb on + strand at 205.174 kb on + strand at 205.348 kb on + strand, within ABIE40_RS20505 at 205.348 kb on + strand, within ABIE40_RS20505 at 205.349 kb on - strand, within ABIE40_RS20505 at 205.419 kb on + strand, within ABIE40_RS20505 at 205.420 kb on - strand, within ABIE40_RS20505 at 205.501 kb on + strand at 205.502 kb on - strand at 205.593 kb on + strand at 205.593 kb on + strand at 205.594 kb on - strand at 205.610 kb on + strand at 205.611 kb on - strand at 205.788 kb on + strand, within ABIE40_RS20510 at 205.788 kb on + strand, within ABIE40_RS20510
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with 6-Pentyl-2H-pyran-2-one 0.835572666666667 mM remove 202,844 - ABIE40_RS20490 0.39 -2.6 202,844 - ABIE40_RS20490 0.39 -2.2 202,844 - ABIE40_RS20490 0.39 -0.3 202,844 - ABIE40_RS20490 0.39 -2.0 202,955 - ABIE40_RS20490 0.46 -0.1 203,089 - ABIE40_RS20490 0.55 +1.1 203,089 - ABIE40_RS20490 0.55 -0.7 203,089 - ABIE40_RS20490 0.55 -2.0 203,200 + ABIE40_RS20490 0.62 +0.1 203,200 + ABIE40_RS20490 0.62 +1.7 203,200 + ABIE40_RS20490 0.62 -0.9 203,201 - ABIE40_RS20490 0.62 -0.2 203,201 - ABIE40_RS20490 0.62 -0.5 203,201 - ABIE40_RS20490 0.62 -0.2 203,296 + ABIE40_RS20490 0.68 +1.1 203,317 + ABIE40_RS20490 0.69 -0.2 203,317 + ABIE40_RS20490 0.69 -1.6 203,318 - ABIE40_RS20490 0.69 +0.1 203,318 - ABIE40_RS20490 0.69 +0.9 203,318 - ABIE40_RS20490 0.69 +2.1 203,494 + ABIE40_RS20490 0.80 +3.1 203,495 - ABIE40_RS20490 0.80 -1.0 203,495 - ABIE40_RS20490 0.80 -0.2 203,683 + -2.0 203,683 + +0.9 203,683 + +0.3 203,683 + -1.5 203,683 + +2.1 203,683 + +0.6 203,684 - +2.4 203,684 - -1.8 203,685 + +1.7 203,686 - +0.1 203,686 - -0.5 203,835 + +1.6 203,835 + -0.6 203,840 + +0.5 203,841 - -0.1 203,841 - +0.6 203,924 + -0.6 203,924 + +0.1 203,990 + ABIE40_RS20495 0.16 +1.5 204,060 + ABIE40_RS20495 0.22 -0.2 204,084 + ABIE40_RS20495 0.24 +0.0 204,084 + ABIE40_RS20495 0.24 -1.7 204,085 - ABIE40_RS20495 0.24 +0.3 204,141 + ABIE40_RS20495 0.29 -0.3 204,142 - ABIE40_RS20495 0.29 -0.9 204,158 + ABIE40_RS20495 0.30 -0.5 204,295 - ABIE40_RS20495 0.42 -0.2 204,327 + ABIE40_RS20495 0.45 -1.5 204,328 - ABIE40_RS20495 0.45 +1.4 204,432 + ABIE40_RS20495 0.54 -1.1 204,432 + ABIE40_RS20495 0.54 +0.1 204,515 + ABIE40_RS20495 0.61 +0.8 204,516 - ABIE40_RS20495 0.61 -0.5 204,516 - ABIE40_RS20495 0.61 +1.0 204,516 - ABIE40_RS20495 0.61 -1.3 204,516 - ABIE40_RS20495 0.61 -0.9 204,516 - ABIE40_RS20495 0.61 -0.2 204,516 - ABIE40_RS20495 0.61 +0.3 204,597 + ABIE40_RS20495 0.68 -1.2 204,598 - ABIE40_RS20495 0.68 -2.9 204,598 - ABIE40_RS20495 0.68 +2.5 204,606 + ABIE40_RS20495 0.69 -1.5 204,607 - ABIE40_RS20495 0.69 +2.1 204,607 - ABIE40_RS20495 0.69 -0.3 204,607 - ABIE40_RS20495 0.69 -0.3 204,607 - ABIE40_RS20495 0.69 +0.6 204,734 + ABIE40_RS20495 0.80 +1.1 204,735 - ABIE40_RS20495 0.80 +4.3 204,824 + ABIE40_RS20495 0.88 +1.1 204,824 + ABIE40_RS20495 0.88 +1.3 204,898 - +0.5 204,898 - +1.0 204,957 + -0.9 204,960 + -0.1 204,960 + -0.5 204,960 + -0.3 204,982 - -0.2 205,044 + ABIE40_RS20500 0.62 +0.4 205,045 - ABIE40_RS20500 0.63 -1.7 205,159 - +1.5 205,159 - -1.1 205,161 + +2.1 205,161 + -1.0 205,174 + -0.2 205,348 + ABIE40_RS20505 0.49 +0.1 205,348 + ABIE40_RS20505 0.49 -1.0 205,349 - ABIE40_RS20505 0.49 -1.4 205,419 + ABIE40_RS20505 0.72 +2.9 205,420 - ABIE40_RS20505 0.72 +0.2 205,501 + -0.5 205,502 - +0.2 205,593 + +0.1 205,593 + +1.1 205,594 - -0.5 205,610 + -0.3 205,611 - +0.6 205,788 + ABIE40_RS20510 0.24 +0.1 205,788 + ABIE40_RS20510 0.24 -0.1
Or see this region's nucleotide sequence