Experiment: R2A with 6-Pentyl-2H-pyran-2-one 0.835572666666667 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE40_RS29640 and ABIE40_RS29645 are separated by 10 nucleotides ABIE40_RS29645 and ABIE40_RS29650 are separated by 43 nucleotides
ABIE40_RS29640: ABIE40_RS29640 - dihydroorotase family protein, at 624,840 to 626,174
_RS29640
ABIE40_RS29645: ABIE40_RS29645 - SDR family oxidoreductase, at 626,185 to 626,958
_RS29645
ABIE40_RS29650: ABIE40_RS29650 - SDR family oxidoreductase, at 627,002 to 627,787
_RS29650
Position (kb)
626
627 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 625.407 kb on - strand, within ABIE40_RS29640 at 625.563 kb on - strand, within ABIE40_RS29640 at 625.563 kb on - strand, within ABIE40_RS29640 at 625.642 kb on - strand, within ABIE40_RS29640 at 625.766 kb on + strand, within ABIE40_RS29640 at 625.766 kb on + strand, within ABIE40_RS29640 at 625.766 kb on + strand, within ABIE40_RS29640 at 625.766 kb on + strand, within ABIE40_RS29640 at 625.766 kb on + strand, within ABIE40_RS29640 at 625.766 kb on + strand, within ABIE40_RS29640 at 625.766 kb on + strand, within ABIE40_RS29640 at 625.936 kb on + strand, within ABIE40_RS29640 at 625.937 kb on - strand, within ABIE40_RS29640 at 625.981 kb on + strand, within ABIE40_RS29640 at 625.981 kb on + strand, within ABIE40_RS29640 at 625.982 kb on - strand, within ABIE40_RS29640 at 625.995 kb on - strand, within ABIE40_RS29640 at 626.048 kb on + strand at 626.049 kb on - strand at 626.049 kb on - strand at 626.049 kb on - strand at 626.049 kb on - strand at 626.049 kb on - strand at 626.049 kb on - strand at 626.169 kb on + strand at 626.169 kb on + strand at 626.170 kb on - strand at 626.172 kb on + strand at 626.172 kb on + strand at 626.173 kb on - strand at 626.184 kb on + strand at 626.184 kb on + strand at 626.185 kb on - strand at 626.185 kb on - strand at 626.185 kb on - strand at 626.185 kb on - strand at 626.185 kb on - strand at 626.192 kb on + strand at 626.192 kb on + strand at 626.255 kb on + strand at 626.255 kb on + strand at 626.256 kb on - strand at 626.260 kb on + strand at 626.261 kb on - strand at 626.384 kb on + strand, within ABIE40_RS29645 at 626.384 kb on + strand, within ABIE40_RS29645 at 626.471 kb on + strand, within ABIE40_RS29645 at 626.471 kb on + strand, within ABIE40_RS29645 at 626.471 kb on + strand, within ABIE40_RS29645 at 626.472 kb on - strand, within ABIE40_RS29645 at 626.472 kb on - strand, within ABIE40_RS29645 at 626.472 kb on - strand, within ABIE40_RS29645 at 626.473 kb on + strand, within ABIE40_RS29645 at 626.473 kb on + strand, within ABIE40_RS29645 at 626.475 kb on + strand, within ABIE40_RS29645 at 626.475 kb on + strand, within ABIE40_RS29645 at 626.475 kb on + strand, within ABIE40_RS29645 at 626.476 kb on - strand, within ABIE40_RS29645 at 626.476 kb on - strand, within ABIE40_RS29645 at 626.476 kb on - strand, within ABIE40_RS29645 at 626.551 kb on + strand, within ABIE40_RS29645 at 626.552 kb on - strand, within ABIE40_RS29645 at 626.552 kb on - strand, within ABIE40_RS29645 at 626.736 kb on + strand, within ABIE40_RS29645 at 626.736 kb on + strand, within ABIE40_RS29645 at 626.818 kb on + strand, within ABIE40_RS29645 at 626.978 kb on - strand at 626.984 kb on + strand at 626.985 kb on - strand at 627.036 kb on + strand at 627.419 kb on - strand, within ABIE40_RS29650 at 627.419 kb on - strand, within ABIE40_RS29650 at 627.584 kb on - strand, within ABIE40_RS29650 at 627.675 kb on - strand, within ABIE40_RS29650 at 627.692 kb on + strand, within ABIE40_RS29650 at 627.693 kb on - strand, within ABIE40_RS29650 at 627.693 kb on - strand, within ABIE40_RS29650 at 627.734 kb on + strand at 627.734 kb on + strand at 627.735 kb on - strand at 627.794 kb on + strand at 627.794 kb on + strand at 627.865 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with 6-Pentyl-2H-pyran-2-one 0.835572666666667 mM remove 625,407 - ABIE40_RS29640 0.42 +2.1 625,563 - ABIE40_RS29640 0.54 +0.5 625,563 - ABIE40_RS29640 0.54 +0.8 625,642 - ABIE40_RS29640 0.60 -1.0 625,766 + ABIE40_RS29640 0.69 +1.0 625,766 + ABIE40_RS29640 0.69 -1.4 625,766 + ABIE40_RS29640 0.69 +1.6 625,766 + ABIE40_RS29640 0.69 -0.9 625,766 + ABIE40_RS29640 0.69 -0.8 625,766 + ABIE40_RS29640 0.69 +1.1 625,766 + ABIE40_RS29640 0.69 -0.8 625,936 + ABIE40_RS29640 0.82 -0.2 625,937 - ABIE40_RS29640 0.82 -0.7 625,981 + ABIE40_RS29640 0.85 -0.2 625,981 + ABIE40_RS29640 0.85 -0.2 625,982 - ABIE40_RS29640 0.86 -0.7 625,995 - ABIE40_RS29640 0.87 -1.1 626,048 + +1.1 626,049 - +0.1 626,049 - -0.2 626,049 - +0.6 626,049 - +0.7 626,049 - +1.6 626,049 - -1.2 626,169 + +0.3 626,169 + +0.4 626,170 - -2.3 626,172 + -0.3 626,172 + -0.3 626,173 - -0.8 626,184 + +1.1 626,184 + -0.5 626,185 - +0.4 626,185 - +0.1 626,185 - +2.1 626,185 - -1.2 626,185 - -1.6 626,192 + +0.3 626,192 + -3.0 626,255 + -1.6 626,255 + +0.8 626,256 - -2.4 626,260 + -0.9 626,261 - -1.7 626,384 + ABIE40_RS29645 0.26 -0.6 626,384 + ABIE40_RS29645 0.26 -0.4 626,471 + ABIE40_RS29645 0.37 -1.4 626,471 + ABIE40_RS29645 0.37 +2.1 626,471 + ABIE40_RS29645 0.37 +0.4 626,472 - ABIE40_RS29645 0.37 -1.4 626,472 - ABIE40_RS29645 0.37 -0.2 626,472 - ABIE40_RS29645 0.37 +0.1 626,473 + ABIE40_RS29645 0.37 -0.4 626,473 + ABIE40_RS29645 0.37 +0.6 626,475 + ABIE40_RS29645 0.37 +0.2 626,475 + ABIE40_RS29645 0.37 +0.8 626,475 + ABIE40_RS29645 0.37 -1.8 626,476 - ABIE40_RS29645 0.38 +1.1 626,476 - ABIE40_RS29645 0.38 -2.7 626,476 - ABIE40_RS29645 0.38 +0.9 626,551 + ABIE40_RS29645 0.47 -0.9 626,552 - ABIE40_RS29645 0.47 +0.9 626,552 - ABIE40_RS29645 0.47 -1.8 626,736 + ABIE40_RS29645 0.71 +2.1 626,736 + ABIE40_RS29645 0.71 -0.3 626,818 + ABIE40_RS29645 0.82 +1.1 626,978 - -2.1 626,984 + +1.3 626,985 - +1.4 627,036 + -0.3 627,419 - ABIE40_RS29650 0.53 -1.4 627,419 - ABIE40_RS29650 0.53 +0.3 627,584 - ABIE40_RS29650 0.74 +2.1 627,675 - ABIE40_RS29650 0.86 +0.8 627,692 + ABIE40_RS29650 0.88 +2.6 627,693 - ABIE40_RS29650 0.88 -1.2 627,693 - ABIE40_RS29650 0.88 -0.4 627,734 + -1.6 627,734 + -0.4 627,735 - -0.9 627,794 + -0.8 627,794 + +0.1 627,865 - -0.8
Or see this region's nucleotide sequence