Experiment: R2A with 6-Pentyl-2H-pyran-2-one 0.835572666666667 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE40_RS18550 and ABIE40_RS18555 overlap by 4 nucleotides ABIE40_RS18555 and ABIE40_RS18560 are separated by 150 nucleotides ABIE40_RS18560 and ABIE40_RS18565 are separated by 54 nucleotides
ABIE40_RS18550: ABIE40_RS18550 - lysine-2,3-aminomutase-like protein, at 3,709,643 to 3,710,695
_RS18550
ABIE40_RS18555: ABIE40_RS18555 - EF-P lysine aminoacylase EpmA, at 3,710,692 to 3,711,756
_RS18555
ABIE40_RS18560: ABIE40_RS18560 - elongation factor P, at 3,711,907 to 3,712,476
_RS18560
ABIE40_RS18565: ABIE40_RS18565 - SIS domain-containing protein, at 3,712,531 to 3,713,259
_RS18565
Position (kb)
3710
3711
3712 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3709.772 kb on - strand, within ABIE40_RS18550 at 3709.773 kb on + strand, within ABIE40_RS18550 at 3709.773 kb on + strand, within ABIE40_RS18550 at 3709.773 kb on + strand, within ABIE40_RS18550 at 3709.774 kb on - strand, within ABIE40_RS18550 at 3709.799 kb on - strand, within ABIE40_RS18550 at 3709.890 kb on + strand, within ABIE40_RS18550 at 3709.890 kb on + strand, within ABIE40_RS18550 at 3709.890 kb on + strand, within ABIE40_RS18550 at 3709.890 kb on + strand, within ABIE40_RS18550 at 3709.894 kb on - strand, within ABIE40_RS18550 at 3710.077 kb on - strand, within ABIE40_RS18550 at 3710.077 kb on - strand, within ABIE40_RS18550 at 3710.288 kb on - strand, within ABIE40_RS18550 at 3710.289 kb on + strand, within ABIE40_RS18550 at 3710.289 kb on + strand, within ABIE40_RS18550 at 3710.289 kb on + strand, within ABIE40_RS18550 at 3710.290 kb on - strand, within ABIE40_RS18550 at 3710.290 kb on - strand, within ABIE40_RS18550 at 3710.516 kb on + strand, within ABIE40_RS18550 at 3710.517 kb on - strand, within ABIE40_RS18550 at 3710.690 kb on - strand at 3710.690 kb on - strand at 3710.743 kb on - strand at 3710.855 kb on + strand, within ABIE40_RS18555 at 3710.855 kb on + strand, within ABIE40_RS18555 at 3710.855 kb on + strand, within ABIE40_RS18555 at 3710.855 kb on + strand, within ABIE40_RS18555 at 3710.856 kb on - strand, within ABIE40_RS18555 at 3710.957 kb on + strand, within ABIE40_RS18555 at 3710.957 kb on + strand, within ABIE40_RS18555 at 3710.958 kb on - strand, within ABIE40_RS18555 at 3710.958 kb on - strand, within ABIE40_RS18555 at 3710.958 kb on - strand, within ABIE40_RS18555 at 3710.958 kb on - strand, within ABIE40_RS18555 at 3710.958 kb on - strand, within ABIE40_RS18555 at 3711.114 kb on + strand, within ABIE40_RS18555 at 3711.114 kb on + strand, within ABIE40_RS18555 at 3711.115 kb on - strand, within ABIE40_RS18555 at 3711.290 kb on + strand, within ABIE40_RS18555 at 3711.291 kb on - strand, within ABIE40_RS18555 at 3711.366 kb on - strand, within ABIE40_RS18555 at 3711.366 kb on - strand, within ABIE40_RS18555 at 3711.366 kb on - strand, within ABIE40_RS18555 at 3711.521 kb on + strand, within ABIE40_RS18555 at 3711.521 kb on + strand, within ABIE40_RS18555 at 3711.522 kb on - strand, within ABIE40_RS18555 at 3711.522 kb on - strand, within ABIE40_RS18555 at 3711.522 kb on - strand, within ABIE40_RS18555 at 3711.522 kb on - strand, within ABIE40_RS18555 at 3711.605 kb on - strand, within ABIE40_RS18555 at 3711.647 kb on + strand, within ABIE40_RS18555 at 3711.816 kb on + strand at 3711.816 kb on + strand at 3711.816 kb on + strand at 3711.816 kb on + strand at 3711.816 kb on + strand at 3711.873 kb on + strand at 3712.613 kb on + strand, within ABIE40_RS18565 at 3712.614 kb on - strand, within ABIE40_RS18565 at 3712.614 kb on - strand, within ABIE40_RS18565 at 3712.614 kb on - strand, within ABIE40_RS18565
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with 6-Pentyl-2H-pyran-2-one 0.835572666666667 mM remove 3,709,772 - ABIE40_RS18550 0.12 -2.3 3,709,773 + ABIE40_RS18550 0.12 -0.2 3,709,773 + ABIE40_RS18550 0.12 -0.1 3,709,773 + ABIE40_RS18550 0.12 +0.7 3,709,774 - ABIE40_RS18550 0.12 -1.9 3,709,799 - ABIE40_RS18550 0.15 +0.6 3,709,890 + ABIE40_RS18550 0.23 -1.4 3,709,890 + ABIE40_RS18550 0.23 -2.2 3,709,890 + ABIE40_RS18550 0.23 +1.2 3,709,890 + ABIE40_RS18550 0.23 -1.3 3,709,894 - ABIE40_RS18550 0.24 -1.0 3,710,077 - ABIE40_RS18550 0.41 +0.3 3,710,077 - ABIE40_RS18550 0.41 +0.0 3,710,288 - ABIE40_RS18550 0.61 -0.9 3,710,289 + ABIE40_RS18550 0.61 -1.5 3,710,289 + ABIE40_RS18550 0.61 -1.4 3,710,289 + ABIE40_RS18550 0.61 -0.7 3,710,290 - ABIE40_RS18550 0.61 -0.1 3,710,290 - ABIE40_RS18550 0.61 -0.4 3,710,516 + ABIE40_RS18550 0.83 -0.9 3,710,517 - ABIE40_RS18550 0.83 -0.9 3,710,690 - -1.1 3,710,690 - -1.9 3,710,743 - +0.3 3,710,855 + ABIE40_RS18555 0.15 +0.3 3,710,855 + ABIE40_RS18555 0.15 +0.9 3,710,855 + ABIE40_RS18555 0.15 +0.9 3,710,855 + ABIE40_RS18555 0.15 +1.9 3,710,856 - ABIE40_RS18555 0.15 -1.4 3,710,957 + ABIE40_RS18555 0.25 -1.5 3,710,957 + ABIE40_RS18555 0.25 -0.4 3,710,958 - ABIE40_RS18555 0.25 +1.9 3,710,958 - ABIE40_RS18555 0.25 +0.3 3,710,958 - ABIE40_RS18555 0.25 +0.3 3,710,958 - ABIE40_RS18555 0.25 -1.1 3,710,958 - ABIE40_RS18555 0.25 -1.1 3,711,114 + ABIE40_RS18555 0.40 +0.9 3,711,114 + ABIE40_RS18555 0.40 +0.3 3,711,115 - ABIE40_RS18555 0.40 -1.3 3,711,290 + ABIE40_RS18555 0.56 -0.1 3,711,291 - ABIE40_RS18555 0.56 +1.9 3,711,366 - ABIE40_RS18555 0.63 -2.0 3,711,366 - ABIE40_RS18555 0.63 -0.7 3,711,366 - ABIE40_RS18555 0.63 -0.7 3,711,521 + ABIE40_RS18555 0.78 +1.9 3,711,521 + ABIE40_RS18555 0.78 +1.9 3,711,522 - ABIE40_RS18555 0.78 -0.1 3,711,522 - ABIE40_RS18555 0.78 -0.1 3,711,522 - ABIE40_RS18555 0.78 -2.2 3,711,522 - ABIE40_RS18555 0.78 +0.3 3,711,605 - ABIE40_RS18555 0.86 -3.0 3,711,647 + ABIE40_RS18555 0.90 -0.9 3,711,816 + +1.6 3,711,816 + -0.9 3,711,816 + -1.0 3,711,816 + -0.9 3,711,816 + -0.4 3,711,873 + +0.3 3,712,613 + ABIE40_RS18565 0.11 +0.3 3,712,614 - ABIE40_RS18565 0.11 +0.2 3,712,614 - ABIE40_RS18565 0.11 +0.1 3,712,614 - ABIE40_RS18565 0.11 +0.8
Or see this region's nucleotide sequence