Experiment: R2A with 6-Pentyl-2H-pyran-2-one 1.00268716666667 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE40_RS05105 and ABIE40_RS05110 are separated by 116 nucleotides ABIE40_RS05110 and ABIE40_RS05115 are separated by 11 nucleotides
ABIE40_RS05105: ABIE40_RS05105 - class I SAM-dependent methyltransferase, at 995,884 to 996,690
_RS05105
ABIE40_RS05110: ABIE40_RS05110 - EAL domain-containing protein, at 996,807 to 998,399
_RS05110
ABIE40_RS05115: ABIE40_RS05115 - DUF1003 domain-containing protein, at 998,411 to 999,316
_RS05115
Position (kb)
996
997
998
999 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 995.853 kb on + strand at 995.854 kb on - strand at 995.856 kb on + strand at 995.856 kb on + strand at 995.856 kb on + strand at 995.857 kb on - strand at 995.857 kb on - strand at 995.857 kb on - strand at 995.866 kb on + strand at 995.866 kb on + strand at 995.867 kb on - strand at 995.867 kb on - strand at 995.867 kb on - strand at 995.893 kb on + strand at 995.893 kb on + strand at 995.950 kb on + strand at 995.950 kb on + strand at 995.950 kb on + strand at 995.987 kb on + strand, within ABIE40_RS05105 at 996.096 kb on + strand, within ABIE40_RS05105 at 996.512 kb on - strand, within ABIE40_RS05105 at 996.512 kb on - strand, within ABIE40_RS05105 at 996.512 kb on - strand, within ABIE40_RS05105 at 996.751 kb on + strand at 996.751 kb on + strand at 996.751 kb on + strand at 996.763 kb on + strand at 996.835 kb on + strand at 996.835 kb on + strand at 996.835 kb on + strand at 996.835 kb on + strand at 996.835 kb on + strand at 996.836 kb on - strand at 996.836 kb on - strand at 996.836 kb on - strand at 996.838 kb on + strand at 996.838 kb on + strand at 996.839 kb on - strand at 996.869 kb on + strand at 996.870 kb on - strand at 996.870 kb on - strand at 997.076 kb on + strand, within ABIE40_RS05110 at 997.076 kb on + strand, within ABIE40_RS05110 at 997.076 kb on + strand, within ABIE40_RS05110 at 997.076 kb on + strand, within ABIE40_RS05110 at 997.077 kb on - strand, within ABIE40_RS05110 at 997.077 kb on - strand, within ABIE40_RS05110 at 997.077 kb on - strand, within ABIE40_RS05110 at 997.176 kb on + strand, within ABIE40_RS05110 at 997.176 kb on + strand, within ABIE40_RS05110 at 997.176 kb on + strand, within ABIE40_RS05110 at 997.176 kb on + strand, within ABIE40_RS05110 at 997.177 kb on - strand, within ABIE40_RS05110 at 997.500 kb on + strand, within ABIE40_RS05110 at 997.500 kb on + strand, within ABIE40_RS05110 at 997.501 kb on - strand, within ABIE40_RS05110 at 997.501 kb on - strand, within ABIE40_RS05110 at 997.501 kb on - strand, within ABIE40_RS05110 at 997.581 kb on + strand, within ABIE40_RS05110 at 997.581 kb on + strand, within ABIE40_RS05110 at 997.582 kb on - strand, within ABIE40_RS05110 at 997.582 kb on - strand, within ABIE40_RS05110 at 997.582 kb on - strand, within ABIE40_RS05110 at 997.582 kb on - strand, within ABIE40_RS05110 at 997.657 kb on - strand, within ABIE40_RS05110 at 998.077 kb on - strand, within ABIE40_RS05110 at 998.091 kb on + strand, within ABIE40_RS05110 at 998.091 kb on + strand, within ABIE40_RS05110 at 998.092 kb on - strand, within ABIE40_RS05110 at 998.092 kb on - strand, within ABIE40_RS05110 at 998.092 kb on - strand, within ABIE40_RS05110 at 998.121 kb on + strand, within ABIE40_RS05110 at 998.259 kb on + strand at 998.259 kb on + strand at 998.416 kb on + strand at 998.416 kb on + strand at 998.416 kb on + strand at 998.417 kb on - strand at 998.417 kb on - strand at 998.417 kb on - strand at 998.646 kb on + strand, within ABIE40_RS05115 at 998.646 kb on + strand, within ABIE40_RS05115 at 998.646 kb on + strand, within ABIE40_RS05115 at 998.646 kb on + strand, within ABIE40_RS05115 at 998.647 kb on - strand, within ABIE40_RS05115 at 998.914 kb on - strand, within ABIE40_RS05115 at 999.023 kb on - strand, within ABIE40_RS05115 at 999.023 kb on - strand, within ABIE40_RS05115 at 999.024 kb on + strand, within ABIE40_RS05115 at 999.024 kb on + strand, within ABIE40_RS05115 at 999.024 kb on + strand, within ABIE40_RS05115 at 999.024 kb on + strand, within ABIE40_RS05115 at 999.024 kb on + strand, within ABIE40_RS05115 at 999.025 kb on - strand, within ABIE40_RS05115 at 999.025 kb on - strand, within ABIE40_RS05115 at 999.025 kb on - strand, within ABIE40_RS05115 at 999.025 kb on - strand, within ABIE40_RS05115 at 999.025 kb on - strand, within ABIE40_RS05115 at 999.025 kb on - strand, within ABIE40_RS05115 at 999.025 kb on - strand, within ABIE40_RS05115 at 999.036 kb on + strand, within ABIE40_RS05115 at 999.037 kb on - strand, within ABIE40_RS05115 at 999.061 kb on + strand, within ABIE40_RS05115 at 999.061 kb on + strand, within ABIE40_RS05115 at 999.061 kb on + strand, within ABIE40_RS05115 at 999.061 kb on + strand, within ABIE40_RS05115 at 999.061 kb on + strand, within ABIE40_RS05115 at 999.061 kb on + strand, within ABIE40_RS05115 at 999.061 kb on + strand, within ABIE40_RS05115 at 999.061 kb on + strand, within ABIE40_RS05115 at 999.061 kb on + strand, within ABIE40_RS05115 at 999.061 kb on + strand, within ABIE40_RS05115 at 999.062 kb on - strand, within ABIE40_RS05115 at 999.062 kb on - strand, within ABIE40_RS05115 at 999.062 kb on - strand, within ABIE40_RS05115 at 999.062 kb on - strand, within ABIE40_RS05115 at 999.062 kb on - strand, within ABIE40_RS05115 at 999.063 kb on + strand, within ABIE40_RS05115 at 999.063 kb on + strand, within ABIE40_RS05115 at 999.063 kb on + strand, within ABIE40_RS05115 at 999.063 kb on + strand, within ABIE40_RS05115 at 999.063 kb on + strand, within ABIE40_RS05115 at 999.063 kb on + strand, within ABIE40_RS05115 at 999.063 kb on + strand, within ABIE40_RS05115 at 999.063 kb on + strand, within ABIE40_RS05115 at 999.063 kb on + strand, within ABIE40_RS05115 at 999.063 kb on + strand, within ABIE40_RS05115 at 999.064 kb on - strand, within ABIE40_RS05115 at 999.064 kb on - strand, within ABIE40_RS05115 at 999.064 kb on - strand, within ABIE40_RS05115 at 999.064 kb on - strand, within ABIE40_RS05115 at 999.064 kb on - strand, within ABIE40_RS05115 at 999.064 kb on - strand, within ABIE40_RS05115 at 999.147 kb on + strand, within ABIE40_RS05115 at 999.225 kb on + strand, within ABIE40_RS05115 at 999.225 kb on + strand, within ABIE40_RS05115 at 999.226 kb on - strand at 999.349 kb on + strand at 999.349 kb on + strand at 999.349 kb on + strand at 999.349 kb on + strand at 999.349 kb on + strand at 999.349 kb on + strand at 999.349 kb on + strand at 999.350 kb on - strand at 999.350 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with 6-Pentyl-2H-pyran-2-one 1.00268716666667 mM remove 995,853 + +0.2 995,854 - +2.0 995,856 + -2.4 995,856 + -0.5 995,856 + +0.4 995,857 - +0.8 995,857 - +1.0 995,857 - -0.4 995,866 + -0.4 995,866 + +1.0 995,867 - -0.5 995,867 - -0.4 995,867 - -1.1 995,893 + -2.0 995,893 + -0.7 995,950 + +0.4 995,950 + +1.1 995,950 + +0.8 995,987 + ABIE40_RS05105 0.13 -1.2 996,096 + ABIE40_RS05105 0.26 -0.6 996,512 - ABIE40_RS05105 0.78 -0.5 996,512 - ABIE40_RS05105 0.78 -0.3 996,512 - ABIE40_RS05105 0.78 -0.0 996,751 + -0.5 996,751 + -0.6 996,751 + -0.9 996,763 + -0.9 996,835 + -0.5 996,835 + -0.2 996,835 + -0.7 996,835 + +1.0 996,835 + -0.4 996,836 - -1.4 996,836 - -1.2 996,836 - -0.0 996,838 + +1.1 996,838 + -0.6 996,839 - -3.2 996,869 + -1.3 996,870 - +1.1 996,870 - -0.0 997,076 + ABIE40_RS05110 0.17 -0.4 997,076 + ABIE40_RS05110 0.17 -0.8 997,076 + ABIE40_RS05110 0.17 +0.6 997,076 + ABIE40_RS05110 0.17 -0.3 997,077 - ABIE40_RS05110 0.17 -3.4 997,077 - ABIE40_RS05110 0.17 -0.6 997,077 - ABIE40_RS05110 0.17 +1.6 997,176 + ABIE40_RS05110 0.23 +1.1 997,176 + ABIE40_RS05110 0.23 +1.1 997,176 + ABIE40_RS05110 0.23 -0.6 997,176 + ABIE40_RS05110 0.23 -1.6 997,177 - ABIE40_RS05110 0.23 +0.4 997,500 + ABIE40_RS05110 0.44 -0.0 997,500 + ABIE40_RS05110 0.44 +0.8 997,501 - ABIE40_RS05110 0.44 -0.7 997,501 - ABIE40_RS05110 0.44 +0.0 997,501 - ABIE40_RS05110 0.44 -0.6 997,581 + ABIE40_RS05110 0.49 -0.8 997,581 + ABIE40_RS05110 0.49 -1.2 997,582 - ABIE40_RS05110 0.49 +1.8 997,582 - ABIE40_RS05110 0.49 +0.6 997,582 - ABIE40_RS05110 0.49 -0.0 997,582 - ABIE40_RS05110 0.49 +0.6 997,657 - ABIE40_RS05110 0.53 +0.4 998,077 - ABIE40_RS05110 0.80 -0.7 998,091 + ABIE40_RS05110 0.81 -0.3 998,091 + ABIE40_RS05110 0.81 +0.2 998,092 - ABIE40_RS05110 0.81 +0.8 998,092 - ABIE40_RS05110 0.81 +0.3 998,092 - ABIE40_RS05110 0.81 -2.9 998,121 + ABIE40_RS05110 0.82 +0.2 998,259 + +1.1 998,259 + -3.6 998,416 + +0.4 998,416 + -1.4 998,416 + -0.5 998,417 - +0.3 998,417 - -0.5 998,417 - -0.5 998,646 + ABIE40_RS05115 0.26 +0.1 998,646 + ABIE40_RS05115 0.26 +0.1 998,646 + ABIE40_RS05115 0.26 -0.3 998,646 + ABIE40_RS05115 0.26 +1.8 998,647 - ABIE40_RS05115 0.26 +1.4 998,914 - ABIE40_RS05115 0.56 +0.2 999,023 - ABIE40_RS05115 0.68 +0.4 999,023 - ABIE40_RS05115 0.68 +3.0 999,024 + ABIE40_RS05115 0.68 -0.5 999,024 + ABIE40_RS05115 0.68 +0.4 999,024 + ABIE40_RS05115 0.68 -1.1 999,024 + ABIE40_RS05115 0.68 +1.1 999,024 + ABIE40_RS05115 0.68 -0.2 999,025 - ABIE40_RS05115 0.68 -1.3 999,025 - ABIE40_RS05115 0.68 -3.3 999,025 - ABIE40_RS05115 0.68 +1.2 999,025 - ABIE40_RS05115 0.68 -0.1 999,025 - ABIE40_RS05115 0.68 -0.6 999,025 - ABIE40_RS05115 0.68 -0.9 999,025 - ABIE40_RS05115 0.68 -1.4 999,036 + ABIE40_RS05115 0.69 -0.0 999,037 - ABIE40_RS05115 0.69 -2.1 999,061 + ABIE40_RS05115 0.72 -0.8 999,061 + ABIE40_RS05115 0.72 -0.6 999,061 + ABIE40_RS05115 0.72 +1.0 999,061 + ABIE40_RS05115 0.72 -1.4 999,061 + ABIE40_RS05115 0.72 -0.4 999,061 + ABIE40_RS05115 0.72 -2.9 999,061 + ABIE40_RS05115 0.72 -0.0 999,061 + ABIE40_RS05115 0.72 -0.7 999,061 + ABIE40_RS05115 0.72 -0.7 999,061 + ABIE40_RS05115 0.72 -0.8 999,062 - ABIE40_RS05115 0.72 +0.4 999,062 - ABIE40_RS05115 0.72 -0.7 999,062 - ABIE40_RS05115 0.72 +0.3 999,062 - ABIE40_RS05115 0.72 -0.4 999,062 - ABIE40_RS05115 0.72 -0.1 999,063 + ABIE40_RS05115 0.72 +0.1 999,063 + ABIE40_RS05115 0.72 -0.3 999,063 + ABIE40_RS05115 0.72 -1.4 999,063 + ABIE40_RS05115 0.72 +1.5 999,063 + ABIE40_RS05115 0.72 -0.1 999,063 + ABIE40_RS05115 0.72 -1.3 999,063 + ABIE40_RS05115 0.72 +0.5 999,063 + ABIE40_RS05115 0.72 -1.1 999,063 + ABIE40_RS05115 0.72 +1.8 999,063 + ABIE40_RS05115 0.72 -0.6 999,064 - ABIE40_RS05115 0.72 -0.0 999,064 - ABIE40_RS05115 0.72 +1.0 999,064 - ABIE40_RS05115 0.72 -0.5 999,064 - ABIE40_RS05115 0.72 -1.4 999,064 - ABIE40_RS05115 0.72 +0.2 999,064 - ABIE40_RS05115 0.72 -0.0 999,147 + ABIE40_RS05115 0.81 -3.0 999,225 + ABIE40_RS05115 0.90 +0.1 999,225 + ABIE40_RS05115 0.90 -2.1 999,226 - -0.6 999,349 + -0.5 999,349 + -0.6 999,349 + -0.9 999,349 + +0.1 999,349 + -0.0 999,349 + -1.8 999,349 + -1.7 999,350 - -2.8 999,350 - -0.9
Or see this region's nucleotide sequence