Experiment: R2A with 6-Pentyl-2H-pyran-2-one 1.00268716666667 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE40_RS03420 and ABIE40_RS03425 are separated by 5 nucleotides ABIE40_RS03425 and ABIE40_RS03430 are separated by 164 nucleotides ABIE40_RS03430 and ABIE40_RS03435 are separated by 247 nucleotides
ABIE40_RS03420: ABIE40_RS03420 - GH25 family lysozyme, at 679,839 to 680,846
_RS03420
ABIE40_RS03425: ABIE40_RS03425 - hypothetical protein, at 680,852 to 681,769
_RS03425
ABIE40_RS03430: ABIE40_RS03430 - tRNA-Gln, at 681,934 to 682,007
_RS03430
ABIE40_RS03435: ABIE40_RS03435 - exopolyphosphatase, at 682,255 to 683,799
_RS03435
Position (kb) 680
681
682 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 679.879 kb on + strand at 679.880 kb on - strand at 680.160 kb on + strand, within ABIE40_RS03420 at 680.160 kb on + strand, within ABIE40_RS03420 at 680.160 kb on + strand, within ABIE40_RS03420 at 680.160 kb on + strand, within ABIE40_RS03420 at 680.160 kb on + strand, within ABIE40_RS03420 at 680.160 kb on + strand, within ABIE40_RS03420 at 680.160 kb on + strand, within ABIE40_RS03420 at 680.161 kb on - strand, within ABIE40_RS03420 at 680.161 kb on - strand, within ABIE40_RS03420 at 680.161 kb on - strand, within ABIE40_RS03420 at 680.161 kb on - strand, within ABIE40_RS03420 at 680.161 kb on - strand, within ABIE40_RS03420 at 680.161 kb on - strand, within ABIE40_RS03420 at 680.161 kb on - strand, within ABIE40_RS03420 at 680.161 kb on - strand, within ABIE40_RS03420 at 680.304 kb on + strand, within ABIE40_RS03420 at 680.304 kb on + strand, within ABIE40_RS03420 at 680.304 kb on + strand, within ABIE40_RS03420 at 680.305 kb on - strand, within ABIE40_RS03420 at 680.305 kb on - strand, within ABIE40_RS03420 at 680.305 kb on - strand, within ABIE40_RS03420 at 680.305 kb on - strand, within ABIE40_RS03420 at 680.306 kb on + strand, within ABIE40_RS03420 at 680.306 kb on + strand, within ABIE40_RS03420 at 680.306 kb on + strand, within ABIE40_RS03420 at 680.306 kb on + strand, within ABIE40_RS03420 at 680.306 kb on + strand, within ABIE40_RS03420 at 680.306 kb on + strand, within ABIE40_RS03420 at 680.306 kb on + strand, within ABIE40_RS03420 at 680.307 kb on - strand, within ABIE40_RS03420 at 680.307 kb on - strand, within ABIE40_RS03420 at 680.307 kb on - strand, within ABIE40_RS03420 at 680.398 kb on - strand, within ABIE40_RS03420 at 680.398 kb on - strand, within ABIE40_RS03420 at 680.409 kb on + strand, within ABIE40_RS03420 at 680.409 kb on + strand, within ABIE40_RS03420 at 680.410 kb on - strand, within ABIE40_RS03420 at 680.410 kb on - strand, within ABIE40_RS03420 at 680.613 kb on + strand, within ABIE40_RS03420 at 680.613 kb on + strand, within ABIE40_RS03420 at 680.614 kb on - strand, within ABIE40_RS03420 at 680.614 kb on - strand, within ABIE40_RS03420 at 680.614 kb on - strand, within ABIE40_RS03420 at 680.614 kb on - strand, within ABIE40_RS03420 at 680.632 kb on - strand, within ABIE40_RS03420 at 680.709 kb on + strand, within ABIE40_RS03420 at 680.853 kb on + strand at 680.853 kb on + strand at 680.853 kb on + strand at 680.854 kb on - strand at 680.854 kb on - strand at 680.855 kb on + strand at 680.855 kb on + strand at 680.855 kb on + strand at 680.855 kb on + strand at 680.855 kb on + strand at 680.855 kb on + strand at 680.855 kb on + strand at 680.855 kb on + strand at 680.855 kb on + strand at 680.855 kb on + strand at 680.855 kb on + strand at 680.855 kb on + strand at 680.855 kb on + strand at 680.855 kb on + strand at 680.856 kb on - strand at 680.856 kb on - strand at 680.856 kb on - strand at 680.856 kb on - strand at 680.856 kb on - strand at 680.856 kb on - strand at 680.856 kb on - strand at 680.856 kb on - strand at 680.856 kb on - strand at 680.856 kb on - strand at 680.958 kb on - strand, within ABIE40_RS03425 at 680.958 kb on - strand, within ABIE40_RS03425 at 680.958 kb on - strand, within ABIE40_RS03425 at 680.958 kb on - strand, within ABIE40_RS03425 at 680.982 kb on + strand, within ABIE40_RS03425 at 680.982 kb on + strand, within ABIE40_RS03425 at 680.982 kb on + strand, within ABIE40_RS03425 at 680.982 kb on + strand, within ABIE40_RS03425 at 680.983 kb on - strand, within ABIE40_RS03425 at 680.983 kb on - strand, within ABIE40_RS03425 at 680.983 kb on - strand, within ABIE40_RS03425 at 680.983 kb on - strand, within ABIE40_RS03425 at 680.983 kb on - strand, within ABIE40_RS03425 at 680.983 kb on - strand, within ABIE40_RS03425 at 681.026 kb on + strand, within ABIE40_RS03425 at 681.027 kb on - strand, within ABIE40_RS03425 at 681.027 kb on - strand, within ABIE40_RS03425 at 681.039 kb on - strand, within ABIE40_RS03425 at 681.046 kb on + strand, within ABIE40_RS03425 at 681.046 kb on + strand, within ABIE40_RS03425 at 681.046 kb on + strand, within ABIE40_RS03425 at 681.046 kb on + strand, within ABIE40_RS03425 at 681.046 kb on + strand, within ABIE40_RS03425 at 681.047 kb on - strand, within ABIE40_RS03425 at 681.047 kb on - strand, within ABIE40_RS03425 at 681.063 kb on - strand, within ABIE40_RS03425 at 681.100 kb on + strand, within ABIE40_RS03425 at 681.100 kb on + strand, within ABIE40_RS03425 at 681.150 kb on + strand, within ABIE40_RS03425 at 681.151 kb on - strand, within ABIE40_RS03425 at 681.151 kb on - strand, within ABIE40_RS03425 at 681.151 kb on - strand, within ABIE40_RS03425 at 681.196 kb on + strand, within ABIE40_RS03425 at 681.197 kb on - strand, within ABIE40_RS03425 at 681.197 kb on - strand, within ABIE40_RS03425 at 681.197 kb on - strand, within ABIE40_RS03425 at 681.197 kb on - strand, within ABIE40_RS03425 at 681.216 kb on - strand, within ABIE40_RS03425 at 681.216 kb on - strand, within ABIE40_RS03425 at 681.254 kb on + strand, within ABIE40_RS03425 at 681.254 kb on + strand, within ABIE40_RS03425 at 681.255 kb on - strand, within ABIE40_RS03425 at 681.268 kb on - strand, within ABIE40_RS03425 at 681.268 kb on - strand, within ABIE40_RS03425 at 681.268 kb on - strand, within ABIE40_RS03425 at 681.289 kb on + strand, within ABIE40_RS03425 at 681.290 kb on - strand, within ABIE40_RS03425 at 681.312 kb on - strand, within ABIE40_RS03425 at 681.336 kb on - strand, within ABIE40_RS03425 at 681.336 kb on - strand, within ABIE40_RS03425 at 681.375 kb on + strand, within ABIE40_RS03425 at 681.375 kb on + strand, within ABIE40_RS03425 at 681.375 kb on + strand, within ABIE40_RS03425 at 681.375 kb on + strand, within ABIE40_RS03425 at 681.375 kb on + strand, within ABIE40_RS03425 at 681.376 kb on - strand, within ABIE40_RS03425 at 681.402 kb on + strand, within ABIE40_RS03425 at 681.427 kb on + strand, within ABIE40_RS03425 at 681.427 kb on + strand, within ABIE40_RS03425 at 681.427 kb on + strand, within ABIE40_RS03425 at 681.427 kb on + strand, within ABIE40_RS03425 at 681.428 kb on - strand, within ABIE40_RS03425 at 681.428 kb on - strand, within ABIE40_RS03425 at 681.428 kb on - strand, within ABIE40_RS03425 at 681.428 kb on - strand, within ABIE40_RS03425 at 681.428 kb on - strand, within ABIE40_RS03425 at 681.428 kb on - strand, within ABIE40_RS03425 at 681.428 kb on - strand, within ABIE40_RS03425 at 681.428 kb on - strand, within ABIE40_RS03425 at 681.428 kb on - strand, within ABIE40_RS03425 at 681.428 kb on - strand, within ABIE40_RS03425 at 681.440 kb on - strand, within ABIE40_RS03425 at 681.484 kb on + strand, within ABIE40_RS03425 at 681.485 kb on - strand, within ABIE40_RS03425 at 681.485 kb on - strand, within ABIE40_RS03425 at 681.524 kb on + strand, within ABIE40_RS03425 at 681.525 kb on - strand, within ABIE40_RS03425 at 681.527 kb on + strand, within ABIE40_RS03425 at 681.527 kb on + strand, within ABIE40_RS03425 at 681.528 kb on - strand, within ABIE40_RS03425 at 681.614 kb on + strand, within ABIE40_RS03425 at 681.614 kb on + strand, within ABIE40_RS03425 at 681.614 kb on + strand, within ABIE40_RS03425 at 681.614 kb on + strand, within ABIE40_RS03425 at 681.615 kb on - strand, within ABIE40_RS03425 at 681.615 kb on - strand, within ABIE40_RS03425 at 681.615 kb on - strand, within ABIE40_RS03425 at 681.617 kb on + strand, within ABIE40_RS03425 at 681.617 kb on + strand, within ABIE40_RS03425 at 681.617 kb on + strand, within ABIE40_RS03425 at 681.618 kb on - strand, within ABIE40_RS03425 at 681.646 kb on - strand, within ABIE40_RS03425 at 681.648 kb on + strand, within ABIE40_RS03425 at 681.658 kb on + strand, within ABIE40_RS03425 at 681.659 kb on - strand, within ABIE40_RS03425 at 681.659 kb on - strand, within ABIE40_RS03425 at 681.673 kb on + strand, within ABIE40_RS03425 at 681.673 kb on + strand, within ABIE40_RS03425 at 681.673 kb on + strand, within ABIE40_RS03425 at 681.673 kb on + strand, within ABIE40_RS03425 at 681.674 kb on - strand, within ABIE40_RS03425 at 681.720 kb on - strand at 681.720 kb on - strand at 681.720 kb on - strand at 681.731 kb on + strand at 681.732 kb on - strand at 681.785 kb on + strand at 681.785 kb on + strand at 681.807 kb on + strand at 681.807 kb on + strand at 681.807 kb on + strand at 681.808 kb on - strand at 681.816 kb on + strand at 681.816 kb on + strand at 681.817 kb on - strand at 681.817 kb on - strand at 681.817 kb on - strand at 681.918 kb on - strand at 681.918 kb on - strand at 682.051 kb on - strand at 682.051 kb on - strand at 682.131 kb on + strand at 682.132 kb on - strand at 682.132 kb on - strand at 682.200 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with 6-Pentyl-2H-pyran-2-one 1.00268716666667 mM remove 679,879 + -0.8 679,880 - +0.9 680,160 + ABIE40_RS03420 0.32 -1.5 680,160 + ABIE40_RS03420 0.32 -0.2 680,160 + ABIE40_RS03420 0.32 -0.4 680,160 + ABIE40_RS03420 0.32 -1.1 680,160 + ABIE40_RS03420 0.32 -1.1 680,160 + ABIE40_RS03420 0.32 +0.5 680,160 + ABIE40_RS03420 0.32 -0.1 680,161 - ABIE40_RS03420 0.32 -0.4 680,161 - ABIE40_RS03420 0.32 +0.3 680,161 - ABIE40_RS03420 0.32 -1.6 680,161 - ABIE40_RS03420 0.32 -0.1 680,161 - ABIE40_RS03420 0.32 +0.2 680,161 - ABIE40_RS03420 0.32 -2.4 680,161 - ABIE40_RS03420 0.32 -0.2 680,161 - ABIE40_RS03420 0.32 -1.2 680,304 + ABIE40_RS03420 0.46 +0.5 680,304 + ABIE40_RS03420 0.46 -0.4 680,304 + ABIE40_RS03420 0.46 +0.1 680,305 - ABIE40_RS03420 0.46 -2.5 680,305 - ABIE40_RS03420 0.46 -0.7 680,305 - ABIE40_RS03420 0.46 -1.1 680,305 - ABIE40_RS03420 0.46 +0.6 680,306 + ABIE40_RS03420 0.46 +0.1 680,306 + ABIE40_RS03420 0.46 -1.7 680,306 + ABIE40_RS03420 0.46 -0.9 680,306 + ABIE40_RS03420 0.46 +1.2 680,306 + ABIE40_RS03420 0.46 +1.1 680,306 + ABIE40_RS03420 0.46 -3.3 680,306 + ABIE40_RS03420 0.46 -1.5 680,307 - ABIE40_RS03420 0.46 +0.6 680,307 - ABIE40_RS03420 0.46 +1.7 680,307 - ABIE40_RS03420 0.46 +0.3 680,398 - ABIE40_RS03420 0.55 -0.7 680,398 - ABIE40_RS03420 0.55 -0.7 680,409 + ABIE40_RS03420 0.57 -2.2 680,409 + ABIE40_RS03420 0.57 -2.5 680,410 - ABIE40_RS03420 0.57 -2.1 680,410 - ABIE40_RS03420 0.57 -1.1 680,613 + ABIE40_RS03420 0.77 +0.3 680,613 + ABIE40_RS03420 0.77 +0.3 680,614 - ABIE40_RS03420 0.77 +0.6 680,614 - ABIE40_RS03420 0.77 -0.5 680,614 - ABIE40_RS03420 0.77 -0.2 680,614 - ABIE40_RS03420 0.77 -1.0 680,632 - ABIE40_RS03420 0.79 -1.1 680,709 + ABIE40_RS03420 0.86 +0.0 680,853 + -1.5 680,853 + -3.0 680,853 + +1.7 680,854 - -0.1 680,854 - -0.2 680,855 + -2.0 680,855 + -0.9 680,855 + -2.1 680,855 + -0.5 680,855 + -1.4 680,855 + +0.3 680,855 + -0.4 680,855 + -0.1 680,855 + -1.0 680,855 + -0.1 680,855 + -0.4 680,855 + -1.5 680,855 + -0.9 680,855 + +0.9 680,856 - -0.5 680,856 - -1.1 680,856 - +0.3 680,856 - +1.5 680,856 - +1.5 680,856 - +0.4 680,856 - -0.1 680,856 - -0.8 680,856 - -1.8 680,856 - -1.4 680,958 - ABIE40_RS03425 0.12 -1.7 680,958 - ABIE40_RS03425 0.12 -0.2 680,958 - ABIE40_RS03425 0.12 -0.8 680,958 - ABIE40_RS03425 0.12 -0.3 680,982 + ABIE40_RS03425 0.14 +0.7 680,982 + ABIE40_RS03425 0.14 -0.2 680,982 + ABIE40_RS03425 0.14 -2.1 680,982 + ABIE40_RS03425 0.14 -2.7 680,983 - ABIE40_RS03425 0.14 -1.0 680,983 - ABIE40_RS03425 0.14 -1.0 680,983 - ABIE40_RS03425 0.14 -2.3 680,983 - ABIE40_RS03425 0.14 -0.5 680,983 - ABIE40_RS03425 0.14 -3.2 680,983 - ABIE40_RS03425 0.14 -0.6 681,026 + ABIE40_RS03425 0.19 -0.5 681,027 - ABIE40_RS03425 0.19 -2.0 681,027 - ABIE40_RS03425 0.19 -0.2 681,039 - ABIE40_RS03425 0.20 -1.1 681,046 + ABIE40_RS03425 0.21 -2.7 681,046 + ABIE40_RS03425 0.21 -2.4 681,046 + ABIE40_RS03425 0.21 -1.2 681,046 + ABIE40_RS03425 0.21 -3.0 681,046 + ABIE40_RS03425 0.21 +0.5 681,047 - ABIE40_RS03425 0.21 +0.5 681,047 - ABIE40_RS03425 0.21 +0.2 681,063 - ABIE40_RS03425 0.23 -0.1 681,100 + ABIE40_RS03425 0.27 -0.7 681,100 + ABIE40_RS03425 0.27 +0.9 681,150 + ABIE40_RS03425 0.32 -1.4 681,151 - ABIE40_RS03425 0.33 +1.6 681,151 - ABIE40_RS03425 0.33 -1.7 681,151 - ABIE40_RS03425 0.33 -0.2 681,196 + ABIE40_RS03425 0.37 +0.5 681,197 - ABIE40_RS03425 0.38 -0.3 681,197 - ABIE40_RS03425 0.38 +0.4 681,197 - ABIE40_RS03425 0.38 -2.6 681,197 - ABIE40_RS03425 0.38 -0.3 681,216 - ABIE40_RS03425 0.40 -1.8 681,216 - ABIE40_RS03425 0.40 +0.3 681,254 + ABIE40_RS03425 0.44 -1.0 681,254 + ABIE40_RS03425 0.44 -1.5 681,255 - ABIE40_RS03425 0.44 -0.4 681,268 - ABIE40_RS03425 0.45 -0.9 681,268 - ABIE40_RS03425 0.45 +0.7 681,268 - ABIE40_RS03425 0.45 -2.8 681,289 + ABIE40_RS03425 0.48 -0.1 681,290 - ABIE40_RS03425 0.48 -0.7 681,312 - ABIE40_RS03425 0.50 -1.4 681,336 - ABIE40_RS03425 0.53 -1.9 681,336 - ABIE40_RS03425 0.53 -1.1 681,375 + ABIE40_RS03425 0.57 -1.0 681,375 + ABIE40_RS03425 0.57 +0.3 681,375 + ABIE40_RS03425 0.57 -1.2 681,375 + ABIE40_RS03425 0.57 -0.3 681,375 + ABIE40_RS03425 0.57 +0.7 681,376 - ABIE40_RS03425 0.57 +4.4 681,402 + ABIE40_RS03425 0.60 +1.1 681,427 + ABIE40_RS03425 0.63 -2.6 681,427 + ABIE40_RS03425 0.63 -0.4 681,427 + ABIE40_RS03425 0.63 -0.5 681,427 + ABIE40_RS03425 0.63 -1.4 681,428 - ABIE40_RS03425 0.63 -0.1 681,428 - ABIE40_RS03425 0.63 +0.8 681,428 - ABIE40_RS03425 0.63 +0.6 681,428 - ABIE40_RS03425 0.63 +0.7 681,428 - ABIE40_RS03425 0.63 -0.3 681,428 - ABIE40_RS03425 0.63 +2.8 681,428 - ABIE40_RS03425 0.63 -0.7 681,428 - ABIE40_RS03425 0.63 +0.9 681,428 - ABIE40_RS03425 0.63 -1.7 681,428 - ABIE40_RS03425 0.63 -0.8 681,440 - ABIE40_RS03425 0.64 -0.7 681,484 + ABIE40_RS03425 0.69 -4.4 681,485 - ABIE40_RS03425 0.69 +0.2 681,485 - ABIE40_RS03425 0.69 +0.8 681,524 + ABIE40_RS03425 0.73 -0.5 681,525 - ABIE40_RS03425 0.73 -1.2 681,527 + ABIE40_RS03425 0.74 -0.7 681,527 + ABIE40_RS03425 0.74 -2.6 681,528 - ABIE40_RS03425 0.74 -0.5 681,614 + ABIE40_RS03425 0.83 -0.0 681,614 + ABIE40_RS03425 0.83 -2.0 681,614 + ABIE40_RS03425 0.83 +0.8 681,614 + ABIE40_RS03425 0.83 +0.5 681,615 - ABIE40_RS03425 0.83 -2.7 681,615 - ABIE40_RS03425 0.83 -1.9 681,615 - ABIE40_RS03425 0.83 -1.2 681,617 + ABIE40_RS03425 0.83 +0.9 681,617 + ABIE40_RS03425 0.83 -1.9 681,617 + ABIE40_RS03425 0.83 -0.4 681,618 - ABIE40_RS03425 0.83 -2.2 681,646 - ABIE40_RS03425 0.86 -1.2 681,648 + ABIE40_RS03425 0.87 -1.7 681,658 + ABIE40_RS03425 0.88 +1.7 681,659 - ABIE40_RS03425 0.88 -0.9 681,659 - ABIE40_RS03425 0.88 +0.1 681,673 + ABIE40_RS03425 0.89 -1.4 681,673 + ABIE40_RS03425 0.89 -2.4 681,673 + ABIE40_RS03425 0.89 +1.3 681,673 + ABIE40_RS03425 0.89 +1.9 681,674 - ABIE40_RS03425 0.90 -0.8 681,720 - -1.0 681,720 - -0.4 681,720 - +1.9 681,731 + -0.3 681,732 - +0.1 681,785 + -2.2 681,785 + +0.3 681,807 + +1.3 681,807 + -0.9 681,807 + -1.2 681,808 - -1.8 681,816 + -0.4 681,816 + -3.0 681,817 - -0.9 681,817 - -1.6 681,817 - -2.9 681,918 - -0.3 681,918 - +1.1 682,051 - -0.9 682,051 - +0.9 682,131 + -0.9 682,132 - +0.9 682,132 - -0.2 682,200 + -0.4
Or see this region's nucleotide sequence