Experiment: R2A with 6-Pentyl-2H-pyran-2-one 1.00268716666667 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE40_RS26880 and ABIE40_RS26885 are separated by 60 nucleotides ABIE40_RS26885 and ABIE40_RS26890 are separated by 295 nucleotides ABIE40_RS26890 and ABIE40_RS26895 are separated by 77 nucleotides
ABIE40_RS26880: ABIE40_RS26880 - hypothetical protein, at 97,111 to 97,518
_RS26880
ABIE40_RS26885: ABIE40_RS26885 - hypothetical protein, at 97,579 to 98,232
_RS26885
ABIE40_RS26890: ABIE40_RS26890 - DUF983 domain-containing protein, at 98,528 to 98,905
_RS26890
ABIE40_RS26895: ABIE40_RS26895 - hypothetical protein, at 98,983 to 99,216
_RS26895
Position (kb)
97
98
99 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 96.665 kb on + strand at 96.666 kb on - strand at 96.693 kb on + strand at 96.694 kb on - strand at 96.694 kb on - strand at 96.694 kb on - strand at 96.694 kb on - strand at 96.743 kb on + strand at 96.743 kb on + strand at 96.883 kb on + strand at 97.042 kb on + strand at 97.042 kb on + strand at 97.043 kb on - strand at 97.043 kb on - strand at 97.043 kb on - strand at 97.043 kb on - strand at 97.043 kb on - strand at 97.060 kb on + strand at 97.061 kb on - strand at 97.066 kb on + strand at 97.066 kb on + strand at 97.067 kb on - strand at 97.067 kb on - strand at 97.067 kb on - strand at 97.067 kb on - strand at 97.067 kb on - strand at 97.140 kb on + strand at 97.142 kb on + strand at 97.142 kb on + strand at 97.142 kb on + strand at 97.143 kb on - strand at 97.143 kb on - strand at 97.143 kb on - strand at 97.143 kb on - strand at 97.143 kb on - strand at 97.308 kb on + strand, within ABIE40_RS26880 at 97.313 kb on + strand, within ABIE40_RS26880 at 97.313 kb on + strand, within ABIE40_RS26880 at 97.313 kb on + strand, within ABIE40_RS26880 at 97.313 kb on + strand, within ABIE40_RS26880 at 97.313 kb on + strand, within ABIE40_RS26880 at 97.314 kb on - strand, within ABIE40_RS26880 at 97.314 kb on - strand, within ABIE40_RS26880 at 97.358 kb on + strand, within ABIE40_RS26880 at 97.378 kb on - strand, within ABIE40_RS26880 at 97.440 kb on + strand, within ABIE40_RS26880 at 97.440 kb on + strand, within ABIE40_RS26880 at 97.481 kb on + strand at 97.482 kb on - strand at 97.525 kb on + strand at 97.525 kb on + strand at 97.525 kb on + strand at 97.525 kb on + strand at 97.526 kb on - strand at 97.526 kb on - strand at 97.526 kb on - strand at 97.767 kb on + strand, within ABIE40_RS26885 at 97.768 kb on - strand, within ABIE40_RS26885 at 97.833 kb on - strand, within ABIE40_RS26885 at 97.939 kb on - strand, within ABIE40_RS26885 at 97.948 kb on + strand, within ABIE40_RS26885 at 97.948 kb on + strand, within ABIE40_RS26885 at 97.949 kb on - strand, within ABIE40_RS26885 at 97.949 kb on - strand, within ABIE40_RS26885 at 97.949 kb on - strand, within ABIE40_RS26885 at 97.949 kb on - strand, within ABIE40_RS26885 at 97.983 kb on + strand, within ABIE40_RS26885 at 97.984 kb on - strand, within ABIE40_RS26885 at 97.995 kb on - strand, within ABIE40_RS26885 at 97.995 kb on - strand, within ABIE40_RS26885 at 98.094 kb on + strand, within ABIE40_RS26885 at 98.147 kb on - strand, within ABIE40_RS26885 at 98.158 kb on + strand, within ABIE40_RS26885 at 98.158 kb on + strand, within ABIE40_RS26885 at 98.158 kb on + strand, within ABIE40_RS26885 at 98.159 kb on - strand, within ABIE40_RS26885 at 98.159 kb on - strand, within ABIE40_RS26885 at 98.159 kb on - strand, within ABIE40_RS26885 at 98.159 kb on - strand, within ABIE40_RS26885 at 98.159 kb on - strand, within ABIE40_RS26885 at 98.159 kb on - strand, within ABIE40_RS26885 at 98.159 kb on - strand, within ABIE40_RS26885 at 98.159 kb on - strand, within ABIE40_RS26885 at 98.159 kb on - strand, within ABIE40_RS26885 at 98.159 kb on - strand, within ABIE40_RS26885 at 98.241 kb on - strand at 98.241 kb on - strand at 98.245 kb on + strand at 98.275 kb on + strand at 98.355 kb on + strand at 98.568 kb on + strand, within ABIE40_RS26890 at 98.568 kb on + strand, within ABIE40_RS26890 at 98.569 kb on - strand, within ABIE40_RS26890 at 98.677 kb on + strand, within ABIE40_RS26890 at 98.885 kb on + strand at 98.885 kb on + strand at 98.885 kb on + strand at 98.885 kb on + strand at 98.885 kb on + strand at 98.886 kb on - strand at 98.886 kb on - strand at 98.985 kb on + strand at 98.985 kb on - strand at 98.985 kb on - strand at 98.985 kb on - strand at 98.990 kb on - strand at 98.990 kb on - strand at 99.199 kb on - strand at 99.207 kb on - strand at 99.207 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with 6-Pentyl-2H-pyran-2-one 1.00268716666667 mM remove 96,665 + +1.4 96,666 - -0.0 96,693 + -0.8 96,694 - +2.4 96,694 - +0.7 96,694 - +0.8 96,694 - +0.4 96,743 + +1.4 96,743 + +1.4 96,883 + -0.7 97,042 + -1.5 97,042 + -2.0 97,043 - -1.9 97,043 - +0.4 97,043 - -0.2 97,043 - +2.4 97,043 - -0.2 97,060 + +0.4 97,061 - -0.4 97,066 + -0.5 97,066 + -0.3 97,067 - +1.0 97,067 - -0.0 97,067 - +0.3 97,067 - +0.8 97,067 - -0.1 97,140 + -1.4 97,142 + +2.4 97,142 + -2.0 97,142 + -1.0 97,143 - -0.3 97,143 - -0.1 97,143 - +2.4 97,143 - +0.8 97,143 - +0.3 97,308 + ABIE40_RS26880 0.48 +0.3 97,313 + ABIE40_RS26880 0.50 -2.6 97,313 + ABIE40_RS26880 0.50 -0.8 97,313 + ABIE40_RS26880 0.50 +0.8 97,313 + ABIE40_RS26880 0.50 -3.0 97,313 + ABIE40_RS26880 0.50 -1.3 97,314 - ABIE40_RS26880 0.50 +1.8 97,314 - ABIE40_RS26880 0.50 +0.4 97,358 + ABIE40_RS26880 0.61 -1.0 97,378 - ABIE40_RS26880 0.65 +2.4 97,440 + ABIE40_RS26880 0.81 -2.3 97,440 + ABIE40_RS26880 0.81 -2.0 97,481 + -0.4 97,482 - -1.5 97,525 + -0.2 97,525 + +1.5 97,525 + -1.4 97,525 + -0.4 97,526 - -0.5 97,526 - -2.0 97,526 - +1.4 97,767 + ABIE40_RS26885 0.29 +2.4 97,768 - ABIE40_RS26885 0.29 -2.0 97,833 - ABIE40_RS26885 0.39 +2.4 97,939 - ABIE40_RS26885 0.55 -3.1 97,948 + ABIE40_RS26885 0.56 +0.8 97,948 + ABIE40_RS26885 0.56 +0.8 97,949 - ABIE40_RS26885 0.57 +0.6 97,949 - ABIE40_RS26885 0.57 -1.4 97,949 - ABIE40_RS26885 0.57 +0.4 97,949 - ABIE40_RS26885 0.57 +2.0 97,983 + ABIE40_RS26885 0.62 +1.4 97,984 - ABIE40_RS26885 0.62 -1.5 97,995 - ABIE40_RS26885 0.64 +1.1 97,995 - ABIE40_RS26885 0.64 +0.0 98,094 + ABIE40_RS26885 0.79 +0.8 98,147 - ABIE40_RS26885 0.87 -1.0 98,158 + ABIE40_RS26885 0.89 +1.6 98,158 + ABIE40_RS26885 0.89 -0.5 98,158 + ABIE40_RS26885 0.89 -0.2 98,159 - ABIE40_RS26885 0.89 +0.1 98,159 - ABIE40_RS26885 0.89 -0.4 98,159 - ABIE40_RS26885 0.89 -2.6 98,159 - ABIE40_RS26885 0.89 +0.4 98,159 - ABIE40_RS26885 0.89 -1.3 98,159 - ABIE40_RS26885 0.89 -0.4 98,159 - ABIE40_RS26885 0.89 +2.4 98,159 - ABIE40_RS26885 0.89 -2.6 98,159 - ABIE40_RS26885 0.89 -0.5 98,159 - ABIE40_RS26885 0.89 -2.1 98,241 - -0.4 98,241 - -1.4 98,245 + -0.2 98,275 + +0.4 98,355 + -1.2 98,568 + ABIE40_RS26890 0.11 +0.9 98,568 + ABIE40_RS26890 0.11 +2.4 98,569 - ABIE40_RS26890 0.11 -0.6 98,677 + ABIE40_RS26890 0.39 +1.4 98,885 + -1.0 98,885 + -1.8 98,885 + -1.6 98,885 + -2.6 98,885 + -1.2 98,886 - +1.4 98,886 - -0.3 98,985 + -2.4 98,985 - -0.4 98,985 - -3.0 98,985 - -0.2 98,990 - -0.4 98,990 - +1.4 99,199 - +0.3 99,207 - +1.4 99,207 - -0.2
Or see this region's nucleotide sequence