Experiment: R2A with 6-Pentyl-2H-pyran-2-one 1.00268716666667 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE40_RS24285 and ABIE40_RS24290 are separated by 34 nucleotides ABIE40_RS24290 and ABIE40_RS24295 are separated by 42 nucleotides ABIE40_RS24295 and ABIE40_RS24300 are separated by 258 nucleotides
ABIE40_RS24285: ABIE40_RS24285 - NAD(P)/FAD-dependent oxidoreductase, at 1,002,603 to 1,003,841
_RS24285
ABIE40_RS24290: ABIE40_RS24290 - alpha/beta hydrolase, at 1,003,876 to 1,004,634
_RS24290
ABIE40_RS24295: ABIE40_RS24295 - cytochrome c biogenesis protein DipZ, at 1,004,677 to 1,006,446
_RS24295
ABIE40_RS24300: ABIE40_RS24300 - alpha/beta hydrolase, at 1,006,705 to 1,007,748
_RS24300
Position (kb)
1004
1005
1006
1007 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 1003.816 kb on + strand at 1003.816 kb on + strand at 1003.817 kb on - strand at 1003.817 kb on - strand at 1003.877 kb on + strand at 1003.878 kb on - strand at 1003.878 kb on - strand at 1003.878 kb on - strand at 1003.878 kb on - strand at 1004.092 kb on - strand, within ABIE40_RS24290 at 1004.195 kb on + strand, within ABIE40_RS24290 at 1004.196 kb on - strand, within ABIE40_RS24290 at 1004.201 kb on + strand, within ABIE40_RS24290 at 1004.201 kb on + strand, within ABIE40_RS24290 at 1004.201 kb on + strand, within ABIE40_RS24290 at 1004.201 kb on + strand, within ABIE40_RS24290 at 1004.201 kb on + strand, within ABIE40_RS24290 at 1004.201 kb on + strand, within ABIE40_RS24290 at 1004.201 kb on + strand, within ABIE40_RS24290 at 1004.202 kb on - strand, within ABIE40_RS24290 at 1004.202 kb on - strand, within ABIE40_RS24290 at 1004.202 kb on - strand, within ABIE40_RS24290 at 1004.202 kb on - strand, within ABIE40_RS24290 at 1004.202 kb on - strand, within ABIE40_RS24290 at 1004.304 kb on - strand, within ABIE40_RS24290 at 1004.360 kb on + strand, within ABIE40_RS24290 at 1004.360 kb on + strand, within ABIE40_RS24290 at 1004.360 kb on + strand, within ABIE40_RS24290 at 1004.360 kb on + strand, within ABIE40_RS24290 at 1004.360 kb on + strand, within ABIE40_RS24290 at 1004.360 kb on + strand, within ABIE40_RS24290 at 1004.360 kb on + strand, within ABIE40_RS24290 at 1004.361 kb on - strand, within ABIE40_RS24290 at 1004.361 kb on - strand, within ABIE40_RS24290 at 1004.361 kb on - strand, within ABIE40_RS24290 at 1004.361 kb on - strand, within ABIE40_RS24290 at 1004.443 kb on + strand, within ABIE40_RS24290 at 1004.548 kb on - strand, within ABIE40_RS24290 at 1004.696 kb on + strand at 1004.697 kb on - strand at 1004.774 kb on + strand at 1004.774 kb on + strand at 1004.933 kb on + strand, within ABIE40_RS24295 at 1004.934 kb on - strand, within ABIE40_RS24295 at 1005.035 kb on + strand, within ABIE40_RS24295 at 1005.102 kb on + strand, within ABIE40_RS24295 at 1005.104 kb on + strand, within ABIE40_RS24295 at 1005.104 kb on + strand, within ABIE40_RS24295 at 1005.104 kb on + strand, within ABIE40_RS24295 at 1005.104 kb on + strand, within ABIE40_RS24295 at 1005.104 kb on + strand, within ABIE40_RS24295 at 1005.104 kb on + strand, within ABIE40_RS24295 at 1005.104 kb on + strand, within ABIE40_RS24295 at 1005.105 kb on - strand, within ABIE40_RS24295 at 1005.105 kb on - strand, within ABIE40_RS24295 at 1005.105 kb on - strand, within ABIE40_RS24295 at 1005.223 kb on - strand, within ABIE40_RS24295 at 1005.266 kb on + strand, within ABIE40_RS24295 at 1005.267 kb on - strand, within ABIE40_RS24295 at 1005.311 kb on + strand, within ABIE40_RS24295 at 1005.311 kb on + strand, within ABIE40_RS24295 at 1005.311 kb on + strand, within ABIE40_RS24295 at 1005.312 kb on - strand, within ABIE40_RS24295 at 1005.312 kb on - strand, within ABIE40_RS24295 at 1005.312 kb on - strand, within ABIE40_RS24295 at 1005.341 kb on + strand, within ABIE40_RS24295 at 1005.342 kb on - strand, within ABIE40_RS24295 at 1005.342 kb on - strand, within ABIE40_RS24295 at 1005.470 kb on + strand, within ABIE40_RS24295 at 1005.471 kb on - strand, within ABIE40_RS24295 at 1005.494 kb on + strand, within ABIE40_RS24295 at 1005.495 kb on - strand, within ABIE40_RS24295 at 1005.495 kb on - strand, within ABIE40_RS24295 at 1005.495 kb on - strand, within ABIE40_RS24295 at 1005.510 kb on + strand, within ABIE40_RS24295 at 1005.527 kb on + strand, within ABIE40_RS24295 at 1005.528 kb on - strand, within ABIE40_RS24295 at 1005.528 kb on - strand, within ABIE40_RS24295 at 1005.776 kb on + strand, within ABIE40_RS24295 at 1005.776 kb on + strand, within ABIE40_RS24295 at 1005.776 kb on + strand, within ABIE40_RS24295 at 1005.777 kb on - strand, within ABIE40_RS24295 at 1005.802 kb on - strand, within ABIE40_RS24295 at 1006.239 kb on - strand, within ABIE40_RS24295 at 1006.424 kb on + strand at 1006.424 kb on + strand at 1006.424 kb on + strand at 1006.425 kb on - strand at 1006.425 kb on - strand at 1006.514 kb on + strand at 1006.515 kb on - strand at 1006.547 kb on + strand at 1006.557 kb on + strand at 1006.577 kb on + strand at 1006.578 kb on - strand at 1006.587 kb on + strand at 1006.588 kb on - strand at 1006.588 kb on - strand at 1006.620 kb on + strand at 1006.620 kb on + strand at 1006.620 kb on + strand at 1006.620 kb on + strand at 1006.620 kb on + strand at 1006.620 kb on + strand at 1006.621 kb on - strand at 1006.621 kb on - strand at 1006.621 kb on - strand at 1006.621 kb on - strand at 1006.621 kb on - strand at 1006.649 kb on + strand at 1006.650 kb on - strand at 1006.650 kb on - strand at 1006.749 kb on - strand at 1006.958 kb on - strand, within ABIE40_RS24300 at 1006.966 kb on + strand, within ABIE40_RS24300 at 1006.966 kb on + strand, within ABIE40_RS24300 at 1006.966 kb on + strand, within ABIE40_RS24300 at 1006.967 kb on - strand, within ABIE40_RS24300 at 1006.967 kb on - strand, within ABIE40_RS24300 at 1006.967 kb on - strand, within ABIE40_RS24300 at 1007.158 kb on + strand, within ABIE40_RS24300 at 1007.184 kb on + strand, within ABIE40_RS24300 at 1007.184 kb on + strand, within ABIE40_RS24300 at 1007.184 kb on + strand, within ABIE40_RS24300 at 1007.185 kb on - strand, within ABIE40_RS24300 at 1007.239 kb on + strand, within ABIE40_RS24300 at 1007.305 kb on + strand, within ABIE40_RS24300 at 1007.306 kb on - strand, within ABIE40_RS24300 at 1007.315 kb on - strand, within ABIE40_RS24300 at 1007.315 kb on - strand, within ABIE40_RS24300 at 1007.315 kb on - strand, within ABIE40_RS24300 at 1007.315 kb on - strand, within ABIE40_RS24300
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with 6-Pentyl-2H-pyran-2-one 1.00268716666667 mM remove 1,003,816 + -0.3 1,003,816 + +1.1 1,003,817 - -0.8 1,003,817 - -1.6 1,003,877 + +2.0 1,003,878 - +1.2 1,003,878 - +0.3 1,003,878 - -1.0 1,003,878 - +2.2 1,004,092 - ABIE40_RS24290 0.28 -0.6 1,004,195 + ABIE40_RS24290 0.42 -1.8 1,004,196 - ABIE40_RS24290 0.42 -0.2 1,004,201 + ABIE40_RS24290 0.43 -0.4 1,004,201 + ABIE40_RS24290 0.43 +0.9 1,004,201 + ABIE40_RS24290 0.43 -0.6 1,004,201 + ABIE40_RS24290 0.43 +2.6 1,004,201 + ABIE40_RS24290 0.43 +0.6 1,004,201 + ABIE40_RS24290 0.43 -0.7 1,004,201 + ABIE40_RS24290 0.43 -0.4 1,004,202 - ABIE40_RS24290 0.43 +0.8 1,004,202 - ABIE40_RS24290 0.43 -0.5 1,004,202 - ABIE40_RS24290 0.43 +1.0 1,004,202 - ABIE40_RS24290 0.43 -1.4 1,004,202 - ABIE40_RS24290 0.43 -1.2 1,004,304 - ABIE40_RS24290 0.56 +0.2 1,004,360 + ABIE40_RS24290 0.64 +0.7 1,004,360 + ABIE40_RS24290 0.64 -1.0 1,004,360 + ABIE40_RS24290 0.64 +0.1 1,004,360 + ABIE40_RS24290 0.64 -0.6 1,004,360 + ABIE40_RS24290 0.64 +0.1 1,004,360 + ABIE40_RS24290 0.64 +1.0 1,004,360 + ABIE40_RS24290 0.64 -0.0 1,004,361 - ABIE40_RS24290 0.64 +0.3 1,004,361 - ABIE40_RS24290 0.64 -1.0 1,004,361 - ABIE40_RS24290 0.64 +0.4 1,004,361 - ABIE40_RS24290 0.64 +0.1 1,004,443 + ABIE40_RS24290 0.75 -1.7 1,004,548 - ABIE40_RS24290 0.89 -0.9 1,004,696 + +0.1 1,004,697 - +1.1 1,004,774 + +1.2 1,004,774 + -0.6 1,004,933 + ABIE40_RS24295 0.14 +0.4 1,004,934 - ABIE40_RS24295 0.15 -0.1 1,005,035 + ABIE40_RS24295 0.20 -0.0 1,005,102 + ABIE40_RS24295 0.24 -0.2 1,005,104 + ABIE40_RS24295 0.24 -1.2 1,005,104 + ABIE40_RS24295 0.24 -0.6 1,005,104 + ABIE40_RS24295 0.24 -2.7 1,005,104 + ABIE40_RS24295 0.24 +0.7 1,005,104 + ABIE40_RS24295 0.24 -1.1 1,005,104 + ABIE40_RS24295 0.24 -0.7 1,005,104 + ABIE40_RS24295 0.24 +0.7 1,005,105 - ABIE40_RS24295 0.24 +2.1 1,005,105 - ABIE40_RS24295 0.24 +1.0 1,005,105 - ABIE40_RS24295 0.24 -0.1 1,005,223 - ABIE40_RS24295 0.31 +1.2 1,005,266 + ABIE40_RS24295 0.33 -0.3 1,005,267 - ABIE40_RS24295 0.33 +0.0 1,005,311 + ABIE40_RS24295 0.36 +1.0 1,005,311 + ABIE40_RS24295 0.36 -0.1 1,005,311 + ABIE40_RS24295 0.36 +0.3 1,005,312 - ABIE40_RS24295 0.36 -0.5 1,005,312 - ABIE40_RS24295 0.36 +0.8 1,005,312 - ABIE40_RS24295 0.36 +4.6 1,005,341 + ABIE40_RS24295 0.38 -2.1 1,005,342 - ABIE40_RS24295 0.38 +1.3 1,005,342 - ABIE40_RS24295 0.38 -0.0 1,005,470 + ABIE40_RS24295 0.45 -2.8 1,005,471 - ABIE40_RS24295 0.45 +0.7 1,005,494 + ABIE40_RS24295 0.46 +0.1 1,005,495 - ABIE40_RS24295 0.46 -2.2 1,005,495 - ABIE40_RS24295 0.46 -0.5 1,005,495 - ABIE40_RS24295 0.46 -1.1 1,005,510 + ABIE40_RS24295 0.47 +1.0 1,005,527 + ABIE40_RS24295 0.48 -0.2 1,005,528 - ABIE40_RS24295 0.48 +2.0 1,005,528 - ABIE40_RS24295 0.48 -0.0 1,005,776 + ABIE40_RS24295 0.62 +0.0 1,005,776 + ABIE40_RS24295 0.62 +0.1 1,005,776 + ABIE40_RS24295 0.62 -1.2 1,005,777 - ABIE40_RS24295 0.62 +0.5 1,005,802 - ABIE40_RS24295 0.64 -1.5 1,006,239 - ABIE40_RS24295 0.88 -1.7 1,006,424 + -2.0 1,006,424 + +0.5 1,006,424 + -1.2 1,006,425 - +0.8 1,006,425 - -0.4 1,006,514 + +2.0 1,006,515 - -0.4 1,006,547 + -1.0 1,006,557 + -0.7 1,006,577 + -0.0 1,006,578 - -0.4 1,006,587 + -0.6 1,006,588 - +2.0 1,006,588 - -0.8 1,006,620 + +0.6 1,006,620 + -0.4 1,006,620 + -1.7 1,006,620 + -1.4 1,006,620 + -0.2 1,006,620 + -0.4 1,006,621 - -0.7 1,006,621 - +1.2 1,006,621 - -1.5 1,006,621 - -1.8 1,006,621 - -1.6 1,006,649 + +0.4 1,006,650 - -2.1 1,006,650 - +0.2 1,006,749 - -3.0 1,006,958 - ABIE40_RS24300 0.24 -2.0 1,006,966 + ABIE40_RS24300 0.25 +1.9 1,006,966 + ABIE40_RS24300 0.25 -0.5 1,006,966 + ABIE40_RS24300 0.25 +0.1 1,006,967 - ABIE40_RS24300 0.25 -3.1 1,006,967 - ABIE40_RS24300 0.25 +0.0 1,006,967 - ABIE40_RS24300 0.25 -1.4 1,007,158 + ABIE40_RS24300 0.43 +0.5 1,007,184 + ABIE40_RS24300 0.46 -0.9 1,007,184 + ABIE40_RS24300 0.46 +3.0 1,007,184 + ABIE40_RS24300 0.46 +0.8 1,007,185 - ABIE40_RS24300 0.46 +2.0 1,007,239 + ABIE40_RS24300 0.51 -0.0 1,007,305 + ABIE40_RS24300 0.57 +0.2 1,007,306 - ABIE40_RS24300 0.58 -0.8 1,007,315 - ABIE40_RS24300 0.58 +1.1 1,007,315 - ABIE40_RS24300 0.58 -0.4 1,007,315 - ABIE40_RS24300 0.58 -0.6 1,007,315 - ABIE40_RS24300 0.58 +0.4
Or see this region's nucleotide sequence