Experiment: R2A with 6-Pentyl-2H-pyran-2-one 1.00268716666667 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE40_RS06680 and ABIE40_RS06685 are separated by 167 nucleotides ABIE40_RS06685 and ABIE40_RS06690 are separated by 71 nucleotides ABIE40_RS06690 and ABIE40_RS06695 are separated by 132 nucleotides ABIE40_RS06695 and ABIE40_RS06700 are separated by 130 nucleotides
ABIE40_RS06680: ABIE40_RS06680 - arginyltransferase, at 1,311,164 to 1,311,940
_RS06680
ABIE40_RS06685: ABIE40_RS06685 - RDD family protein, at 1,312,108 to 1,312,566
_RS06685
ABIE40_RS06690: ABIE40_RS06690 - porphobilinogen synthase, at 1,312,638 to 1,313,651
_RS06690
ABIE40_RS06695: ABIE40_RS06695 - DUF6163 family protein, at 1,313,784 to 1,314,221
_RS06695
ABIE40_RS06700: ABIE40_RS06700 - MarR family winged helix-turn-helix transcriptional regulator, at 1,314,352 to 1,314,867
_RS06700
Position (kb)
1312
1313
1314 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1311.668 kb on - strand, within ABIE40_RS06680 at 1311.765 kb on + strand, within ABIE40_RS06680 at 1311.765 kb on + strand, within ABIE40_RS06680 at 1311.766 kb on - strand, within ABIE40_RS06680 at 1311.766 kb on - strand, within ABIE40_RS06680 at 1311.808 kb on + strand, within ABIE40_RS06680 at 1311.879 kb on + strand at 1311.906 kb on + strand at 1311.906 kb on + strand at 1311.934 kb on + strand at 1311.934 kb on + strand at 1311.934 kb on + strand at 1311.935 kb on - strand at 1311.935 kb on - strand at 1311.989 kb on + strand at 1311.989 kb on + strand at 1311.989 kb on + strand at 1312.031 kb on + strand at 1312.045 kb on - strand at 1312.056 kb on - strand at 1312.097 kb on + strand at 1312.098 kb on - strand at 1312.098 kb on - strand at 1312.098 kb on - strand at 1312.098 kb on - strand at 1312.098 kb on - strand at 1312.349 kb on + strand, within ABIE40_RS06685 at 1312.349 kb on + strand, within ABIE40_RS06685 at 1312.350 kb on - strand, within ABIE40_RS06685 at 1312.350 kb on - strand, within ABIE40_RS06685 at 1312.383 kb on - strand, within ABIE40_RS06685 at 1312.469 kb on + strand, within ABIE40_RS06685 at 1312.469 kb on + strand, within ABIE40_RS06685 at 1312.469 kb on + strand, within ABIE40_RS06685 at 1312.469 kb on + strand, within ABIE40_RS06685 at 1312.470 kb on - strand, within ABIE40_RS06685 at 1312.490 kb on - strand, within ABIE40_RS06685 at 1312.515 kb on - strand, within ABIE40_RS06685 at 1312.515 kb on - strand, within ABIE40_RS06685 at 1312.541 kb on + strand at 1312.542 kb on - strand at 1312.568 kb on + strand at 1312.568 kb on + strand at 1312.568 kb on + strand at 1312.568 kb on + strand at 1312.568 kb on + strand at 1312.568 kb on + strand at 1312.569 kb on - strand at 1312.569 kb on - strand at 1312.569 kb on - strand at 1312.569 kb on - strand at 1312.569 kb on - strand at 1312.569 kb on - strand at 1312.599 kb on + strand at 1312.600 kb on - strand at 1312.600 kb on - strand at 1313.691 kb on + strand at 1313.691 kb on + strand at 1313.692 kb on - strand at 1313.692 kb on - strand at 1313.771 kb on + strand at 1313.771 kb on + strand at 1313.772 kb on - strand at 1313.772 kb on - strand at 1313.772 kb on - strand at 1313.816 kb on + strand at 1313.816 kb on + strand at 1313.817 kb on - strand at 1313.889 kb on + strand, within ABIE40_RS06695 at 1313.890 kb on - strand, within ABIE40_RS06695 at 1314.148 kb on - strand, within ABIE40_RS06695 at 1314.148 kb on - strand, within ABIE40_RS06695 at 1314.148 kb on - strand, within ABIE40_RS06695 at 1314.148 kb on - strand, within ABIE40_RS06695 at 1314.202 kb on + strand at 1314.214 kb on + strand at 1314.215 kb on - strand at 1314.222 kb on + strand at 1314.241 kb on + strand at 1314.253 kb on - strand at 1314.269 kb on + strand at 1314.278 kb on - strand at 1314.283 kb on + strand at 1314.424 kb on + strand, within ABIE40_RS06700 at 1314.424 kb on + strand, within ABIE40_RS06700 at 1314.595 kb on + strand, within ABIE40_RS06700 at 1314.595 kb on + strand, within ABIE40_RS06700 at 1314.616 kb on + strand, within ABIE40_RS06700 at 1314.616 kb on + strand, within ABIE40_RS06700
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with 6-Pentyl-2H-pyran-2-one 1.00268716666667 mM remove 1,311,668 - ABIE40_RS06680 0.65 -0.6 1,311,765 + ABIE40_RS06680 0.77 -0.0 1,311,765 + ABIE40_RS06680 0.77 -1.6 1,311,766 - ABIE40_RS06680 0.77 -0.4 1,311,766 - ABIE40_RS06680 0.77 -0.2 1,311,808 + ABIE40_RS06680 0.83 -0.6 1,311,879 + -0.0 1,311,906 + +1.0 1,311,906 + +1.1 1,311,934 + +0.9 1,311,934 + +1.0 1,311,934 + -3.9 1,311,935 - -0.0 1,311,935 - -3.1 1,311,989 + +1.6 1,311,989 + -0.7 1,311,989 + +0.9 1,312,031 + -3.3 1,312,045 - +0.6 1,312,056 - -0.7 1,312,097 + -1.2 1,312,098 - +0.6 1,312,098 - -0.1 1,312,098 - -1.0 1,312,098 - +0.9 1,312,098 - +1.0 1,312,349 + ABIE40_RS06685 0.53 -0.5 1,312,349 + ABIE40_RS06685 0.53 -1.1 1,312,350 - ABIE40_RS06685 0.53 +0.5 1,312,350 - ABIE40_RS06685 0.53 +0.1 1,312,383 - ABIE40_RS06685 0.60 -1.1 1,312,469 + ABIE40_RS06685 0.79 -0.5 1,312,469 + ABIE40_RS06685 0.79 -0.9 1,312,469 + ABIE40_RS06685 0.79 -1.2 1,312,469 + ABIE40_RS06685 0.79 -0.6 1,312,470 - ABIE40_RS06685 0.79 +0.9 1,312,490 - ABIE40_RS06685 0.83 +0.8 1,312,515 - ABIE40_RS06685 0.89 +0.3 1,312,515 - ABIE40_RS06685 0.89 -0.8 1,312,541 + -2.5 1,312,542 - +0.6 1,312,568 + +0.1 1,312,568 + -1.3 1,312,568 + +1.1 1,312,568 + +1.9 1,312,568 + -0.4 1,312,568 + +1.0 1,312,569 - -0.3 1,312,569 - -0.4 1,312,569 - +1.9 1,312,569 - +0.1 1,312,569 - -0.9 1,312,569 - +1.4 1,312,599 + +0.4 1,312,600 - -0.3 1,312,600 - +0.3 1,313,691 + -0.4 1,313,691 + -0.5 1,313,692 - +0.7 1,313,692 - -0.5 1,313,771 + -1.1 1,313,771 + +0.0 1,313,772 - -0.6 1,313,772 - -0.4 1,313,772 - -2.3 1,313,816 + -1.2 1,313,816 + -1.0 1,313,817 - -1.6 1,313,889 + ABIE40_RS06695 0.24 -2.5 1,313,890 - ABIE40_RS06695 0.24 -0.9 1,314,148 - ABIE40_RS06695 0.83 +0.2 1,314,148 - ABIE40_RS06695 0.83 -2.5 1,314,148 - ABIE40_RS06695 0.83 -0.6 1,314,148 - ABIE40_RS06695 0.83 -2.3 1,314,202 + -1.7 1,314,214 + -1.6 1,314,215 - -3.4 1,314,222 + +0.5 1,314,241 + -0.6 1,314,253 - +0.9 1,314,269 + -0.1 1,314,278 - +0.4 1,314,283 + +0.9 1,314,424 + ABIE40_RS06700 0.14 +0.9 1,314,424 + ABIE40_RS06700 0.14 +1.9 1,314,595 + ABIE40_RS06700 0.47 -0.1 1,314,595 + ABIE40_RS06700 0.47 -0.1 1,314,616 + ABIE40_RS06700 0.51 +0.4 1,314,616 + ABIE40_RS06700 0.51 +0.4
Or see this region's nucleotide sequence