Experiment: R2A with 6-Pentyl-2H-pyran-2-one 1.00268716666667 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE40_RS26020 and ABIE40_RS26025 are separated by 232 nucleotides ABIE40_RS26025 and ABIE40_RS26030 are separated by 61 nucleotides ABIE40_RS26030 and ABIE40_RS26035 overlap by 4 nucleotides ABIE40_RS26035 and ABIE40_RS26040 are separated by 75 nucleotides
ABIE40_RS26020: ABIE40_RS26020 - amidase, at 1,386,472 to 1,387,839
_RS26020
ABIE40_RS26025: ABIE40_RS26025 - hypothetical protein, at 1,388,072 to 1,388,347
_RS26025
ABIE40_RS26030: ABIE40_RS26030 - DUF1810 domain-containing protein, at 1,388,409 to 1,388,846
_RS26030
ABIE40_RS26035: ABIE40_RS26035 - hypothetical protein, at 1,388,843 to 1,389,031
_RS26035
ABIE40_RS26040: ABIE40_RS26040 - TetR/AcrR family transcriptional regulator, at 1,389,107 to 1,389,730
_RS26040
Position (kb)
1388
1389 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1387.650 kb on + strand, within ABIE40_RS26020 at 1387.651 kb on - strand, within ABIE40_RS26020 at 1387.651 kb on - strand, within ABIE40_RS26020 at 1387.792 kb on - strand at 1387.826 kb on + strand at 1387.827 kb on - strand at 1387.827 kb on - strand at 1387.827 kb on - strand at 1387.876 kb on - strand at 1387.876 kb on - strand at 1387.902 kb on - strand at 1387.917 kb on - strand at 1387.923 kb on + strand at 1387.967 kb on + strand at 1387.968 kb on - strand at 1387.989 kb on + strand at 1387.990 kb on - strand at 1387.990 kb on - strand at 1387.990 kb on - strand at 1388.037 kb on + strand at 1388.037 kb on + strand at 1388.037 kb on + strand at 1388.037 kb on + strand at 1388.037 kb on + strand at 1388.038 kb on - strand at 1388.154 kb on + strand, within ABIE40_RS26025 at 1388.318 kb on + strand, within ABIE40_RS26025 at 1388.381 kb on + strand at 1388.381 kb on + strand at 1388.466 kb on + strand, within ABIE40_RS26030 at 1388.466 kb on + strand, within ABIE40_RS26030 at 1388.575 kb on - strand, within ABIE40_RS26030 at 1388.790 kb on + strand, within ABIE40_RS26030 at 1388.790 kb on + strand, within ABIE40_RS26030 at 1388.790 kb on + strand, within ABIE40_RS26030 at 1388.790 kb on + strand, within ABIE40_RS26030 at 1388.790 kb on + strand, within ABIE40_RS26030 at 1388.791 kb on - strand, within ABIE40_RS26030 at 1388.791 kb on - strand, within ABIE40_RS26030 at 1388.791 kb on - strand, within ABIE40_RS26030 at 1388.791 kb on - strand, within ABIE40_RS26030 at 1388.791 kb on - strand, within ABIE40_RS26030 at 1388.791 kb on - strand, within ABIE40_RS26030 at 1388.791 kb on - strand, within ABIE40_RS26030 at 1388.844 kb on + strand at 1388.844 kb on + strand at 1388.844 kb on + strand at 1389.003 kb on + strand, within ABIE40_RS26035 at 1389.003 kb on + strand, within ABIE40_RS26035 at 1389.003 kb on + strand, within ABIE40_RS26035 at 1389.004 kb on - strand, within ABIE40_RS26035 at 1389.018 kb on + strand at 1389.018 kb on + strand at 1389.019 kb on - strand at 1389.019 kb on - strand at 1389.019 kb on - strand at 1389.019 kb on - strand at 1389.024 kb on + strand at 1389.024 kb on + strand at 1389.024 kb on + strand at 1389.024 kb on + strand at 1389.024 kb on + strand at 1389.024 kb on + strand at 1389.024 kb on + strand at 1389.024 kb on + strand at 1389.024 kb on + strand at 1389.025 kb on - strand at 1389.025 kb on - strand at 1389.025 kb on - strand at 1389.025 kb on - strand at 1389.025 kb on - strand at 1389.108 kb on + strand at 1389.109 kb on - strand at 1389.109 kb on - strand at 1389.114 kb on + strand at 1389.221 kb on - strand, within ABIE40_RS26040 at 1389.316 kb on - strand, within ABIE40_RS26040 at 1389.540 kb on + strand, within ABIE40_RS26040 at 1389.640 kb on + strand, within ABIE40_RS26040 at 1389.641 kb on - strand, within ABIE40_RS26040 at 1389.641 kb on - strand, within ABIE40_RS26040
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with 6-Pentyl-2H-pyran-2-one 1.00268716666667 mM remove 1,387,650 + ABIE40_RS26020 0.86 +0.8 1,387,651 - ABIE40_RS26020 0.86 +0.0 1,387,651 - ABIE40_RS26020 0.86 -0.5 1,387,792 - +0.5 1,387,826 + +0.8 1,387,827 - +0.2 1,387,827 - +0.6 1,387,827 - -3.1 1,387,876 - +0.9 1,387,876 - -1.0 1,387,902 - +0.4 1,387,917 - -1.7 1,387,923 + -2.6 1,387,967 + -0.9 1,387,968 - -2.6 1,387,989 + +0.5 1,387,990 - -2.4 1,387,990 - -0.7 1,387,990 - -0.5 1,388,037 + -1.3 1,388,037 + -1.2 1,388,037 + -0.8 1,388,037 + +0.0 1,388,037 + +0.2 1,388,038 - +1.4 1,388,154 + ABIE40_RS26025 0.30 -1.3 1,388,318 + ABIE40_RS26025 0.89 +0.5 1,388,381 + -2.4 1,388,381 + -2.2 1,388,466 + ABIE40_RS26030 0.13 +0.1 1,388,466 + ABIE40_RS26030 0.13 +1.6 1,388,575 - ABIE40_RS26030 0.38 -0.6 1,388,790 + ABIE40_RS26030 0.87 -0.3 1,388,790 + ABIE40_RS26030 0.87 +2.6 1,388,790 + ABIE40_RS26030 0.87 -0.2 1,388,790 + ABIE40_RS26030 0.87 -0.6 1,388,790 + ABIE40_RS26030 0.87 +0.5 1,388,791 - ABIE40_RS26030 0.87 -1.0 1,388,791 - ABIE40_RS26030 0.87 +0.5 1,388,791 - ABIE40_RS26030 0.87 -0.8 1,388,791 - ABIE40_RS26030 0.87 +0.5 1,388,791 - ABIE40_RS26030 0.87 -1.9 1,388,791 - ABIE40_RS26030 0.87 -1.1 1,388,791 - ABIE40_RS26030 0.87 +2.2 1,388,844 + -1.2 1,388,844 + +0.4 1,388,844 + -1.8 1,389,003 + ABIE40_RS26035 0.85 +0.8 1,389,003 + ABIE40_RS26035 0.85 +1.8 1,389,003 + ABIE40_RS26035 0.85 -0.3 1,389,004 - ABIE40_RS26035 0.85 -0.6 1,389,018 + -0.3 1,389,018 + +0.9 1,389,019 - +0.3 1,389,019 - +1.7 1,389,019 - -0.8 1,389,019 - +0.8 1,389,024 + +0.5 1,389,024 + -2.2 1,389,024 + +1.1 1,389,024 + -0.4 1,389,024 + +0.0 1,389,024 + +0.3 1,389,024 + +0.7 1,389,024 + -1.3 1,389,024 + +0.2 1,389,025 - -2.5 1,389,025 - +1.8 1,389,025 - +0.8 1,389,025 - -2.5 1,389,025 - -0.2 1,389,108 + -0.7 1,389,109 - +0.7 1,389,109 - +2.5 1,389,114 + +0.2 1,389,221 - ABIE40_RS26040 0.18 -0.3 1,389,316 - ABIE40_RS26040 0.33 -3.5 1,389,540 + ABIE40_RS26040 0.69 -0.2 1,389,640 + ABIE40_RS26040 0.85 +0.8 1,389,641 - ABIE40_RS26040 0.86 -2.0 1,389,641 - ABIE40_RS26040 0.86 -0.2
Or see this region's nucleotide sequence