Experiment: EV240
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt OKFHMN_03425 and potC are separated by 166 nucleotides potC and potD overlap by 4 nucleotides potD and cobB are separated by 155 nucleotides cobB and nagK are separated by 15 nucleotides
OKFHMN_03425: OKFHMN_03425 - integrase, at 533,369 to 534,487
_03425
OKFHMN_03430: potC - spermidine/putrescine ABC transporter permease PotC, at 534,654 to 535,448
potC
OKFHMN_03435: potD - spermidine/putrescine ABC transporter substrate-binding protein PotD, at 535,445 to 536,491
potD
OKFHMN_03440: cobB - NAD-dependent protein deacylase, at 536,647 to 537,468
cobB
OKFHMN_03445: nagK - N-acetylglucosamine kinase, at 537,484 to 538,395
nagK
Position (kb)
535
536
537 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 534.481 kb on + strand at 534.482 kb on - strand at 534.497 kb on + strand at 535.010 kb on + strand, within potC at 535.010 kb on + strand, within potC at 535.360 kb on + strand, within potC at 535.532 kb on + strand at 535.600 kb on + strand, within potD at 535.673 kb on + strand, within potD at 535.697 kb on + strand, within potD at 535.697 kb on + strand, within potD at 535.700 kb on + strand, within potD at 536.071 kb on + strand, within potD at 536.908 kb on + strand, within cobB at 536.909 kb on - strand, within cobB at 536.952 kb on + strand, within cobB at 536.953 kb on - strand, within cobB at 537.049 kb on - strand, within cobB at 537.076 kb on + strand, within cobB at 537.376 kb on - strand, within cobB
Per-strain Table
Position Strand Gene LocusTag Fraction EV240 remove 534,481 + +0.5 534,482 - -0.8 534,497 + +0.9 535,010 + potC OKFHMN_03430 0.45 +1.5 535,010 + potC OKFHMN_03430 0.45 +0.3 535,360 + potC OKFHMN_03430 0.89 -3.2 535,532 + -1.0 535,600 + potD OKFHMN_03435 0.15 +2.2 535,673 + potD OKFHMN_03435 0.22 -0.1 535,697 + potD OKFHMN_03435 0.24 -0.9 535,697 + potD OKFHMN_03435 0.24 +0.1 535,700 + potD OKFHMN_03435 0.24 +0.4 536,071 + potD OKFHMN_03435 0.60 -0.4 536,908 + cobB OKFHMN_03440 0.32 -0.1 536,909 - cobB OKFHMN_03440 0.32 -1.3 536,952 + cobB OKFHMN_03440 0.37 -2.4 536,953 - cobB OKFHMN_03440 0.37 -2.8 537,049 - cobB OKFHMN_03440 0.49 -1.0 537,076 + cobB OKFHMN_03440 0.52 +0.4 537,376 - cobB OKFHMN_03440 0.89 -0.6
Or see this region's nucleotide sequence