Experiment: EV219
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ycgM and ycgN are separated by 91 nucleotides ycgN and hlyE are separated by 206 nucleotides hlyE and umuD are separated by 372 nucleotides umuD and umuC overlap by 1 nucleotides
OKFHMN_28240: ycgM - fumarylacetoacetate hydrolase family protein, at 5,416,866 to 5,417,525
ycgM
OKFHMN_28245: ycgN - YcgN family cysteine cluster protein, at 5,417,617 to 5,418,063
ycgN
OKFHMN_28250: hlyE - hemolysin HlyE, at 5,418,270 to 5,419,181
hlyE
OKFHMN_28260: umuD - Protein UmuD, at 5,419,554 to 5,419,973
umuD
OKFHMN_28265: umuC - DNA polymerase V catalytic protein, at 5,419,973 to 5,421,241
umuC
Position (kb) 5418
5419
5420 Strain fitness (log2 ratio) -2
-1
0
1 at 5417.320 kb on + strand, within ycgM at 5417.391 kb on - strand, within ycgM at 5417.582 kb on - strand at 5417.627 kb on + strand at 5418.100 kb on + strand at 5418.174 kb on + strand at 5419.490 kb on + strand at 5419.520 kb on + strand at 5419.707 kb on + strand, within umuD at 5419.708 kb on - strand, within umuD at 5419.805 kb on + strand, within umuD
Per-strain Table
Position Strand Gene LocusTag Fraction EV219 remove 5,417,320 + ycgM OKFHMN_28240 0.69 -2.6 5,417,391 - ycgM OKFHMN_28240 0.80 +0.1 5,417,582 - +1.1 5,417,627 + -1.7 5,418,100 + +0.7 5,418,174 + +0.8 5,419,490 + -0.4 5,419,520 + +0.5 5,419,707 + umuD OKFHMN_28260 0.36 -1.6 5,419,708 - umuD OKFHMN_28260 0.37 -0.0 5,419,805 + umuD OKFHMN_28260 0.60 +0.9
Or see this region's nucleotide sequence