Experiment: LB_plus_SM_buffer with Ffm_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF855 and GFF856 are separated by 4 nucleotides GFF856 and GFF857 are separated by 41 nucleotides GFF857 and GFF858 are separated by 147 nucleotides GFF858 and GFF859 are separated by 49 nucleotides GFF859 and GFF860 are separated by 219 nucleotides
GFF855 - Primosomal replication protein N, at 34,412 to 34,579
GFF855
GFF856 - SSU ribosomal protein S18p @ SSU ribosomal protein S18p, zinc-independent, at 34,584 to 34,811
GFF856
GFF857 - LSU ribosomal protein L9p, at 34,853 to 35,302
GFF857
GFF858 - Permease of the drug/metabolite transporter (DMT) superfamily, at 35,450 to 36,376
GFF858
GFF859 - Putative cell envelope opacity-associated protein A, at 36,426 to 37,061
GFF859
GFF860 - FKBP-type peptidyl-prolyl cis-trans isomerase FklB (EC 5.2.1.8), at 37,281 to 37,901
GFF860
Position (kb) 35
36
37 Strain fitness (log2 ratio) -1
0
1
2 at 35.646 kb on + strand, within GFF858 at 35.647 kb on - strand, within GFF858 at 35.748 kb on - strand, within GFF858 at 35.829 kb on + strand, within GFF858 at 35.950 kb on + strand, within GFF858 at 35.950 kb on + strand, within GFF858 at 36.084 kb on + strand, within GFF858 at 36.126 kb on + strand, within GFF858 at 36.126 kb on + strand, within GFF858 at 36.126 kb on + strand, within GFF858 at 36.127 kb on - strand, within GFF858 at 36.314 kb on + strand at 36.315 kb on - strand at 36.428 kb on - strand at 36.496 kb on - strand, within GFF859 at 36.604 kb on + strand, within GFF859 at 36.974 kb on + strand, within GFF859 at 37.066 kb on + strand at 37.066 kb on + strand at 37.066 kb on + strand at 37.067 kb on - strand at 37.099 kb on - strand at 37.103 kb on + strand at 37.120 kb on - strand at 37.125 kb on - strand at 37.131 kb on - strand at 37.158 kb on - strand at 37.158 kb on - strand at 37.169 kb on - strand at 37.280 kb on - strand at 37.323 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 15 MOI remove 35,646 + GFF858 0.21 -0.0 35,647 - GFF858 0.21 -0.6 35,748 - GFF858 0.32 -0.3 35,829 + GFF858 0.41 -0.4 35,950 + GFF858 0.54 +0.1 35,950 + GFF858 0.54 -1.1 36,084 + GFF858 0.68 -0.3 36,126 + GFF858 0.73 -0.3 36,126 + GFF858 0.73 -0.9 36,126 + GFF858 0.73 -0.1 36,127 - GFF858 0.73 -0.2 36,314 + -0.1 36,315 - +0.2 36,428 - -0.0 36,496 - GFF859 0.11 +0.3 36,604 + GFF859 0.28 +0.3 36,974 + GFF859 0.86 +0.9 37,066 + -0.0 37,066 + +0.8 37,066 + +0.7 37,067 - -0.2 37,099 - -0.1 37,103 + +0.1 37,120 - +0.4 37,125 - +0.4 37,131 - +0.4 37,158 - -0.8 37,158 - -0.1 37,169 - +0.5 37,280 - +0.1 37,323 + +1.8
Or see this region's nucleotide sequence