Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF847

Experiment: LB_plus_SM_buffer with Ffm_phage 15 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF846 and GFF847 are separated by 362 nucleotidesGFF847 and GFF848 are separated by 15 nucleotidesGFF848 and GFF849 are separated by 9 nucleotidesGFF849 and GFF850 are separated by 13 nucleotides GFF846 - Probable L-ascorbate-6-phosphate lactonase UlaG (EC 3.1.1.-) (L-ascorbate utilization protein G), at 27,155 to 28,219 GFF846 GFF847 - Ascorbate-specific PTS system, EIIC component, at 28,582 to 29,979 GFF847 GFF848 - Ascorbate-specific PTS system, EIIB component (EC 2.7.1.69), at 29,995 to 30,300 GFF848 GFF849 - Ascorbate-specific PTS system, EIIA component (EC 2.7.1.-), at 30,310 to 30,774 GFF849 GFF850 - 3-keto-L-gulonate-6-phosphate decarboxylase UlaD (EC 4.1.1.85) (L-ascorbate utilization protein D), at 30,788 to 31,438 GFF850 Position (kb) 28 29 30Strain fitness (log2 ratio) -2 -1 0 1 2 3at 27.585 kb on + strand, within GFF846at 27.589 kb on + strand, within GFF846at 27.600 kb on - strand, within GFF846at 27.600 kb on - strand, within GFF846at 27.827 kb on + strand, within GFF846at 27.828 kb on - strand, within GFF846at 28.046 kb on + strand, within GFF846at 28.046 kb on + strand, within GFF846at 28.046 kb on + strand, within GFF846at 28.047 kb on - strand, within GFF846at 28.102 kb on + strand, within GFF846at 28.595 kb on - strandat 28.673 kb on - strandat 28.674 kb on + strandat 28.746 kb on - strand, within GFF847at 28.811 kb on - strand, within GFF847at 28.811 kb on - strand, within GFF847at 28.840 kb on + strand, within GFF847at 28.840 kb on + strand, within GFF847at 28.841 kb on - strand, within GFF847at 28.884 kb on + strand, within GFF847at 28.884 kb on + strand, within GFF847at 28.885 kb on - strand, within GFF847at 28.926 kb on + strand, within GFF847at 28.927 kb on - strand, within GFF847at 29.026 kb on + strand, within GFF847at 29.026 kb on + strand, within GFF847at 29.058 kb on + strand, within GFF847at 29.098 kb on - strand, within GFF847at 29.113 kb on + strand, within GFF847at 29.113 kb on + strand, within GFF847at 29.114 kb on - strand, within GFF847at 29.114 kb on - strand, within GFF847at 29.191 kb on + strand, within GFF847at 29.192 kb on - strand, within GFF847at 29.194 kb on - strand, within GFF847at 29.297 kb on + strand, within GFF847at 29.308 kb on + strand, within GFF847at 29.386 kb on - strand, within GFF847at 29.445 kb on + strand, within GFF847at 29.446 kb on - strand, within GFF847at 29.446 kb on - strand, within GFF847at 29.446 kb on - strand, within GFF847at 29.533 kb on - strand, within GFF847at 29.572 kb on - strand, within GFF847at 29.641 kb on - strand, within GFF847at 29.641 kb on - strand, within GFF847at 29.907 kb on + strandat 29.908 kb on - strandat 29.908 kb on - strandat 29.908 kb on - strandat 30.002 kb on + strandat 30.002 kb on + strandat 30.006 kb on + strandat 30.006 kb on + strandat 30.007 kb on - strandat 30.007 kb on - strandat 30.172 kb on - strand, within GFF848at 30.205 kb on + strand, within GFF848at 30.298 kb on + strandat 30.369 kb on + strand, within GFF849at 30.772 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 15 MOI
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27,585 + GFF846 0.40 +0.2
27,589 + GFF846 0.41 -0.0
27,600 - GFF846 0.42 +0.5
27,600 - GFF846 0.42 +0.0
27,827 + GFF846 0.63 +0.2
27,828 - GFF846 0.63 +0.4
28,046 + GFF846 0.84 +0.0
28,046 + GFF846 0.84 -0.4
28,046 + GFF846 0.84 -0.1
28,047 - GFF846 0.84 -0.1
28,102 + GFF846 0.89 -0.8
28,595 - -1.2
28,673 - -0.1
28,674 + +0.1
28,746 - GFF847 0.12 +0.9
28,811 - GFF847 0.16 -0.6
28,811 - GFF847 0.16 -0.2
28,840 + GFF847 0.18 -0.7
28,840 + GFF847 0.18 -0.9
28,841 - GFF847 0.19 +0.0
28,884 + GFF847 0.22 -0.6
28,884 + GFF847 0.22 -0.3
28,885 - GFF847 0.22 -0.3
28,926 + GFF847 0.25 +0.5
28,927 - GFF847 0.25 -2.4
29,026 + GFF847 0.32 +2.9
29,026 + GFF847 0.32 -0.6
29,058 + GFF847 0.34 -0.3
29,098 - GFF847 0.37 -0.1
29,113 + GFF847 0.38 +0.1
29,113 + GFF847 0.38 -0.6
29,114 - GFF847 0.38 -0.4
29,114 - GFF847 0.38 -0.6
29,191 + GFF847 0.44 -0.4
29,192 - GFF847 0.44 -0.1
29,194 - GFF847 0.44 -0.3
29,297 + GFF847 0.51 +0.1
29,308 + GFF847 0.52 +1.6
29,386 - GFF847 0.58 +0.0
29,445 + GFF847 0.62 +0.5
29,446 - GFF847 0.62 +0.3
29,446 - GFF847 0.62 +0.7
29,446 - GFF847 0.62 -0.1
29,533 - GFF847 0.68 +0.5
29,572 - GFF847 0.71 -0.5
29,641 - GFF847 0.76 +0.4
29,641 - GFF847 0.76 -0.3
29,907 + +0.3
29,908 - -0.2
29,908 - -1.1
29,908 - +0.3
30,002 + +0.4
30,002 + -0.3
30,006 + -0.2
30,006 + +1.0
30,007 - -1.3
30,007 - -0.0
30,172 - GFF848 0.58 -0.3
30,205 + GFF848 0.69 +0.0
30,298 + -0.3
30,369 + GFF849 0.13 +1.1
30,772 + -0.4

Or see this region's nucleotide sequence