Experiment: LB_plus_SM_buffer with Ffm_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF823 and GFF824 are separated by 37 nucleotides GFF824 and GFF825 are separated by 82 nucleotides GFF825 and GFF826 are separated by 71 nucleotides
GFF823 - DNA mismatch repair protein MutL, at 5,043 to 6,899
GFF823
GFF824 - tRNA dimethylallyltransferase (EC 2.5.1.75), at 6,937 to 7,842
GFF824
GFF825 - RNA-binding protein Hfq, at 7,925 to 8,233
GFF825
GFF826 - GTP-binding protein HflX, at 8,305 to 9,585
GFF826
Position (kb)
6
7
8 Strain fitness (log2 ratio)
-2
-1
0
1 at 6.009 kb on + strand, within GFF823 at 6.009 kb on + strand, within GFF823 at 6.010 kb on - strand, within GFF823 at 6.010 kb on - strand, within GFF823 at 6.286 kb on + strand, within GFF823 at 6.344 kb on + strand, within GFF823 at 6.345 kb on - strand, within GFF823 at 6.468 kb on - strand, within GFF823 at 6.468 kb on - strand, within GFF823 at 6.634 kb on + strand, within GFF823 at 6.679 kb on + strand, within GFF823 at 6.684 kb on + strand, within GFF823 at 6.827 kb on - strand at 6.851 kb on - strand at 6.902 kb on + strand at 6.903 kb on - strand at 6.921 kb on + strand at 6.975 kb on - strand at 6.998 kb on + strand at 7.007 kb on + strand at 7.107 kb on - strand, within GFF824 at 7.212 kb on + strand, within GFF824 at 7.347 kb on - strand, within GFF824 at 7.660 kb on + strand, within GFF824 at 7.660 kb on + strand, within GFF824 at 7.681 kb on + strand, within GFF824 at 7.682 kb on - strand, within GFF824 at 7.686 kb on + strand, within GFF824 at 7.811 kb on + strand at 7.814 kb on + strand at 7.849 kb on - strand at 7.912 kb on + strand at 8.015 kb on - strand, within GFF825 at 8.089 kb on + strand, within GFF825 at 8.167 kb on + strand, within GFF825 at 8.168 kb on - strand, within GFF825 at 8.171 kb on + strand, within GFF825 at 8.232 kb on - strand at 8.290 kb on + strand at 8.290 kb on + strand at 8.291 kb on - strand at 8.291 kb on - strand at 8.354 kb on - strand at 8.487 kb on + strand, within GFF826
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 15 MOI remove 6,009 + GFF823 0.52 +0.2 6,009 + GFF823 0.52 +0.1 6,010 - GFF823 0.52 -0.1 6,010 - GFF823 0.52 -0.2 6,286 + GFF823 0.67 -0.8 6,344 + GFF823 0.70 -0.3 6,345 - GFF823 0.70 -0.2 6,468 - GFF823 0.77 -0.7 6,468 - GFF823 0.77 -0.0 6,634 + GFF823 0.86 -0.3 6,679 + GFF823 0.88 +0.6 6,684 + GFF823 0.88 +1.1 6,827 - +0.1 6,851 - -0.8 6,902 + +0.5 6,903 - -1.3 6,921 + -0.4 6,975 - +0.2 6,998 + -0.8 7,007 + -1.4 7,107 - GFF824 0.19 -2.3 7,212 + GFF824 0.30 -2.1 7,347 - GFF824 0.45 -1.8 7,660 + GFF824 0.80 -0.1 7,660 + GFF824 0.80 -0.4 7,681 + GFF824 0.82 -1.9 7,682 - GFF824 0.82 -0.4 7,686 + GFF824 0.83 -1.0 7,811 + +0.1 7,814 + +0.9 7,849 - -1.8 7,912 + -0.9 8,015 - GFF825 0.29 -0.4 8,089 + GFF825 0.53 +0.3 8,167 + GFF825 0.78 +0.2 8,168 - GFF825 0.79 +0.4 8,171 + GFF825 0.80 -1.8 8,232 - -0.8 8,290 + +0.5 8,290 + -1.8 8,291 - +0.2 8,291 - -0.1 8,354 - -0.8 8,487 + GFF826 0.14 +0.4
Or see this region's nucleotide sequence