Experiment: LB_plus_SM_buffer with Ffm_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF745 and GFF746 are separated by 71 nucleotides GFF746 and GFF747 are separated by 312 nucleotides GFF747 and GFF748 are separated by 53 nucleotides GFF748 and GFF749 overlap by 4 nucleotides
GFF745 - Deoxyribose operon repressor, DeoR family, at 103,808 to 104,566
GFF745
GFF746 - Putative permease, at 104,638 to 105,246
GFF746
GFF747 - Multidrug translocase MdfA, at 105,559 to 106,791
GFF747
GFF748 - Protein ybjI, at 106,845 to 107,660
GFF748
GFF749 - Putative transport protein/putative regulator, at 107,657 to 108,868
GFF749
Position (kb)
105
106
107 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 104.566 kb on + strand at 104.583 kb on + strand at 104.617 kb on + strand at 104.617 kb on + strand at 104.618 kb on - strand at 104.788 kb on - strand, within GFF746 at 104.803 kb on + strand, within GFF746 at 104.804 kb on - strand, within GFF746 at 105.312 kb on + strand at 105.343 kb on - strand at 105.388 kb on + strand at 105.393 kb on + strand at 105.430 kb on + strand at 105.431 kb on - strand at 105.558 kb on + strand at 105.632 kb on - strand at 105.642 kb on + strand at 105.647 kb on - strand at 105.698 kb on - strand, within GFF747 at 105.754 kb on - strand, within GFF747 at 105.761 kb on + strand, within GFF747 at 105.883 kb on - strand, within GFF747 at 105.937 kb on + strand, within GFF747 at 106.328 kb on - strand, within GFF747 at 106.404 kb on + strand, within GFF747 at 106.440 kb on + strand, within GFF747 at 106.495 kb on + strand, within GFF747 at 106.496 kb on - strand, within GFF747 at 106.496 kb on - strand, within GFF747 at 106.496 kb on - strand, within GFF747 at 106.524 kb on - strand, within GFF747 at 106.663 kb on - strand, within GFF747 at 106.873 kb on - strand at 106.876 kb on - strand at 106.890 kb on - strand at 107.106 kb on + strand, within GFF748 at 107.272 kb on + strand, within GFF748 at 107.515 kb on + strand, within GFF748 at 107.573 kb on - strand, within GFF748 at 107.599 kb on + strand at 107.776 kb on - strand at 107.776 kb on - strand at 107.790 kb on + strand, within GFF749
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 15 MOI remove 104,566 + -0.0 104,583 + +0.5 104,617 + -0.1 104,617 + -0.3 104,618 - +0.3 104,788 - GFF746 0.25 +1.5 104,803 + GFF746 0.27 -0.8 104,804 - GFF746 0.27 +0.4 105,312 + +0.5 105,343 - +2.0 105,388 + -0.8 105,393 + -0.6 105,430 + +0.2 105,431 - -0.9 105,558 + -1.0 105,632 - +1.0 105,642 + +0.2 105,647 - +0.1 105,698 - GFF747 0.11 -0.0 105,754 - GFF747 0.16 +0.6 105,761 + GFF747 0.16 -1.2 105,883 - GFF747 0.26 -0.3 105,937 + GFF747 0.31 +0.8 106,328 - GFF747 0.62 -0.7 106,404 + GFF747 0.69 -0.0 106,440 + GFF747 0.71 -2.4 106,495 + GFF747 0.76 -0.7 106,496 - GFF747 0.76 -0.8 106,496 - GFF747 0.76 -1.0 106,496 - GFF747 0.76 +0.4 106,524 - GFF747 0.78 -1.3 106,663 - GFF747 0.90 -0.2 106,873 - +0.0 106,876 - +1.4 106,890 - +0.1 107,106 + GFF748 0.32 -0.2 107,272 + GFF748 0.52 -2.8 107,515 + GFF748 0.82 +0.1 107,573 - GFF748 0.89 +0.3 107,599 + +0.3 107,776 - -0.0 107,776 - +0.3 107,790 + GFF749 0.11 -0.1
Or see this region's nucleotide sequence