Experiment: LB_plus_SM_buffer with Ffm_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF689 and GFF690 are separated by 132 nucleotides GFF690 and GFF691 overlap by 39 nucleotides GFF691 and GFF692 are separated by 115 nucleotides
GFF689 - Endonuclease/Exonuclease/phosphatase family protein, at 45,414 to 46,172
GFF689
GFF690 - Putative inner membrane protein, at 46,305 to 46,715
GFF690
GFF691 - ABC transport system, permease component YbhR, at 46,677 to 47,783
GFF691
GFF692 - ABC transport system, permease component YbhS, at 47,899 to 49,029
GFF692
Position (kb) 46
47
48 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 45.712 kb on - strand, within GFF689 at 45.712 kb on - strand, within GFF689 at 45.751 kb on - strand, within GFF689 at 45.802 kb on + strand, within GFF689 at 45.907 kb on + strand, within GFF689 at 46.208 kb on - strand at 46.208 kb on - strand at 46.436 kb on - strand, within GFF690 at 46.706 kb on - strand at 46.805 kb on + strand, within GFF691 at 46.911 kb on - strand, within GFF691 at 46.911 kb on - strand, within GFF691 at 46.993 kb on + strand, within GFF691 at 47.290 kb on + strand, within GFF691 at 47.357 kb on - strand, within GFF691 at 47.550 kb on + strand, within GFF691 at 47.550 kb on + strand, within GFF691 at 47.551 kb on - strand, within GFF691 at 47.616 kb on - strand, within GFF691 at 47.626 kb on + strand, within GFF691 at 47.627 kb on - strand, within GFF691 at 47.645 kb on + strand, within GFF691 at 47.733 kb on + strand at 47.769 kb on + strand at 47.808 kb on - strand at 47.891 kb on - strand at 47.896 kb on + strand at 47.897 kb on - strand at 47.983 kb on + strand at 48.038 kb on + strand, within GFF692 at 48.054 kb on + strand, within GFF692 at 48.055 kb on - strand, within GFF692 at 48.056 kb on + strand, within GFF692 at 48.056 kb on + strand, within GFF692 at 48.084 kb on + strand, within GFF692 at 48.297 kb on + strand, within GFF692 at 48.340 kb on - strand, within GFF692 at 48.462 kb on + strand, within GFF692 at 48.611 kb on + strand, within GFF692 at 48.654 kb on + strand, within GFF692
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 15 MOI remove 45,712 - GFF689 0.39 +0.4 45,712 - GFF689 0.39 -0.1 45,751 - GFF689 0.44 +0.2 45,802 + GFF689 0.51 +0.4 45,907 + GFF689 0.65 +0.0 46,208 - -0.5 46,208 - +0.2 46,436 - GFF690 0.32 -0.9 46,706 - +0.3 46,805 + GFF691 0.12 -1.3 46,911 - GFF691 0.21 -3.0 46,911 - GFF691 0.21 -0.3 46,993 + GFF691 0.29 +1.0 47,290 + GFF691 0.55 +0.4 47,357 - GFF691 0.61 -0.5 47,550 + GFF691 0.79 -1.0 47,550 + GFF691 0.79 -1.2 47,551 - GFF691 0.79 +0.7 47,616 - GFF691 0.85 -0.6 47,626 + GFF691 0.86 -0.2 47,627 - GFF691 0.86 +0.1 47,645 + GFF691 0.87 -0.9 47,733 + +0.2 47,769 + -0.2 47,808 - -1.0 47,891 - +0.2 47,896 + +0.4 47,897 - -0.0 47,983 + +1.6 48,038 + GFF692 0.12 +0.2 48,054 + GFF692 0.14 +0.0 48,055 - GFF692 0.14 +0.5 48,056 + GFF692 0.14 -0.2 48,056 + GFF692 0.14 -0.2 48,084 + GFF692 0.16 +0.0 48,297 + GFF692 0.35 -0.3 48,340 - GFF692 0.39 -0.2 48,462 + GFF692 0.50 -0.1 48,611 + GFF692 0.63 -0.4 48,654 + GFF692 0.67 -0.2
Or see this region's nucleotide sequence